Immuno-Oncology Characterization and Verification Tools

Detailed analysis, characterization, and verification is critical to on-going success with any experiment. Learn more about workflow solutions for immuno-oncology research, from targeted genetic analysis through protein analysis.

Explore our characterization and verification solutions

Targeted genetic analysis

Confirm and quantify genetic changes for a wide range of selected DNA targets that are key to your research.

Genomic or viral DNA isolation

Isolate the highest-quality genomic or viral DNA from a range of sample types for use in all common molecular biology applications.

Fluorometric DNA quantification

Qubit 4 Fluorometer—single sample per run
Varioskan LUX Multimode Microplate Reader—up 1536 samples per run
Varioskan ALF Multimode Microplate Reader—up to 384 samples per run
Quant-iT DNA Quantification kits—multiple samples

UV-Vis Spectrophotometric DNA quantification

NanoDrop One/OneC Microvolume UV-Vis Spectrophotometer with Wi-Fi—single sample per run
Multiskan SkyHigh Microplate Spectrophotometer with µDrop plate for microvolume analysis—up to 16 microvolume samples or up to 384 samples per run
Varioskan ALF Multimode Microplate Reader with µDrop plate for microvolume analysis— up to 16 microvolume samples or up to 384 samples per run
Varioskan LUX Multimode Microplate Reader with µDrop plate for microvolume analysis—up to 16 microvolume samples or up to 384 samples per run

Endpoint PCR

Amplify DNA sequences for subsequent molecular biology applications.

Step 1: Amplify target DNA	Step 2: Analyze
SimpliAmp, Veriti, and ProFlex thermal cyclers MicroAmp plastic consumables Invitrogen custom DNA oligos Platinum Taq DNA Polymerase and Platinum SuperFi DNA Polymerase	E-Gel Power Snap Electrophoresis System E-Gel CloneWell II, SizeSelect II, and EX agarose Gels TrackIt ladders UltraPure Agarose SYBR Safe dyes

Real-time PCR

Accurately analyze specific DNA targets.

Step 1: Prepare samples
We provide multiple solutions to isolate and purify DNA. Kits for genomic DNA extraction—Explore our broad range of kits for purifying genomic DNA from a variety of samples including tissue, cells, blood, serum, plants, forensic samples, and more. DNA Extract All Reagents Kit—Obtain PCR-ready DNA from a wide variety of sample types, ranging from blood to buccal swabs to plant tissues in five minutes.
Step 2: Select assays
We offer a variety of TaqMan Genotyping Assays for a number of real-time PCR analysis applications. TaqMan SNP Genotyping Assays—The Predesigned TaqMan SNP Genotyping Assays collection includes millions of genome‑wide human assays, including common 1,000 Genome SNPs, HapMap SNPs, and coding SNP assay. Find assay › Custom TaqMan SNP Genotyping Assays—Create custom assays by submitting confidential target sequences to our secure assay design pipeline. Design assay › TaqMan Drug Metabolism Assays—A comprehensive collection of inventoried assays optimized for genotyping SNPs, insertions and deletions (indels), and multi‑nucleotide polymorphisms (MNPs) in drug metabolism-related genes.
Step 3: Set up reactions
Prepare a reaction plate using TaqMan Assays and TaqMan master mix, along with your DNA samples. No additional reagents are needed. TaqPath ProAmp Master Mix—Delivers accurate genotyping results, even from samples containing PCR inhibitors. Includes ROX passive reference dye.
Step 4: Run PCR
Amplify DNA and achieve allelic discrimination by running the PCR reaction on an Applied Biosystems real-time PCR system and/or thermal cycler. To better evaluate the accuracy of genotype calls, perform real-time time PCR and obtain genotyping data for every cycle. Or for higher throughput, run PCR on a thermal cycler and then perform an endpoint read on a real-time instrument. Thermocycler instruments ProFlex PCR System—The ProFlex PCR System’s interchangeable block formats allow you to maximize your throughput or run independent experiments concurrently. SimpliAmp Thermal Cycler—The SimpliAmp Thermal Cycler is an easy-to use, compact, and accurate thermal cycler designed to fit every lab’s essential PCR workflow. Real-time PCR instruments Compare instruments › QuantStudio 3 Real-Time PCR System—Simple to use for all levels of experience QuantStudio 5 Real-Time PCR System—Easy controls for optimized experiments QuantStudio 6 Flex Real-Time PCR System—High flexibility and intuitive design QuantStudio 7 Flex Real-Time PCR System—High versatility and intuitive design QuantStudio 12k Flex Real-Time PCR System—Maximum throughput, flexibility, and scalability
Step 5: Analyze data
Process and interpret qPCR data with intuitive software data analysis tools for a range of applications. Applied Biosystems qPCR Analysis Modules—Our cloud-based applications can bring together multiple data sets, with analysis speeds up to 10 times faster than our desktop software. The Genotyping app includes improved visuals and integrated traces of allelic discrimination plots to allow thorough QC of SNP assays to accurately reflect the true signals versus background noise. TaqMan Genotyper Software—TaqMan Genotyper Software is for use with TaqMan SNP Genotyping Assays (Predesigned, Custom & DME) in combination with 48-, 96-, and 384-well microplates or OpenArray Genotyping Plates, and offers a state-of-the-art genotype-calling algorithm, an intuitive user interface, and enhanced multi-plate analysis features.

Sanger sequencing

Verify DNA sequence rapidly using proven DNA sequencing technology.

Step 1: PCR amplification	Step 2: PCR clean-up
AmpliTaq DNA Polymerase Primer Designer Tool for PCR and Sanger sequencing	ExoSAP-IT Express reagent
Step 3: Cycle sequencing	Step 4: Sequencing clean-up
BigDye Terminator cycle and direct cycle sequencing kits	BigDye XTerminator Purification Kit
Step 5: Sequence	Step 6: Analyze data
SeqStudio Genetic Analyzer Applied Biosystems 3500 Genetic Analyzer	Applied Biosystems Sanger Analysis modules Minor Variant Finder Software

Next-generation Sequencing (NGS)

Sequence hundreds to thousands of targets at high depth.

Need help with your project? Request a quote ›

Step 1: Select targets	Step 2: Construct library and/or prepare template
Oncomine TCR Beta-LR Assay Oncomine TCR Beta-SR Assay, DNA Oncomine TCR Beta-SR Assay, RNA Oncomine Immune Response Research Assay Oncomine Tumor Mutation Load Assay Oncomine assays for immuno-oncology and liquid biopsy research Ion AmpliSeq Designer tool for custom designs Learn more about Ion AmpliSeq panels ›	Ion Chef System Ion AmpliSeq kit for Chef DL8
Step 3: Sequence	Step 4: Analyze data
Ion GeneStudio S5 systems	Torrent Suite Software Ion Reporter Software Oncomine Reporter

Step 1: Select targets

Step 2: Construct library and/or prepare template

Learn more about Ion AmpliSeq panels ›

Step 3: Sequence

Step 4: Analyze data

Ion GeneStudio S5 systems

Targeted gene expression analysis

Confirm and quantify RNA targets at the gene, exon, or noncoding RNA level.

RNA Isolation

Isolate the highest-quality cellular RNA, viral RNA, or miRNA from a range of sample types for direct use in all common molecular biology applications

Fluorometric DNA quantification

Qubit 4 Fluorometer—single sample per run
Varioskan LUX Multimode Microplate Reader—up 1536 samples per run
Varioskan ALF Multimode Microplate Reader—up to 384 samples per run
Quant-iT DNA Quantification kits—multiple samples

UV-Vis Spectrophotometric DNA quantification

NanoDrop One/OneC Microvolume UV-Vis Spectrophotometer with Wi-Fi—single sample per run
Multiskan SkyHigh Microplate Spectrophotometer with µDrop plate for microvolume analysis—up to 16 microvolume samples or up to 384 samples per run
Varioskan ALF Multimode Microplate Reader with µDrop plate for microvolume analysis— up to 16 microvolume samples or up to 384 samples per run
Varioskan LUX Multimode Microplate Reader with µDrop plate for microvolume analysis—up to 16 microvolume samples or up to 384 samples per run

RT- PCR

Confirm and compare gene expression for small sample numbers by RT-PCR

Step 1: Synthesize and amplify cDNA	Step 2: Analyze data
Invitrogen custom DNA oligos Invitrogen Value oligos SuperScript IV One-Step RT-PCR System SuperScript IV One-Step RT-PCR System with ezDNase Enzyme MicroAmp plastic consumables SimpliAmp, Veriti, and ProFlex thermal cyclers	E-Gel Power Snap Electrophoresis System E-Gel CloneWell II, SizeSelect II, and EX agarose gels TrackIt ladders UltraPure Agarose SYBR Safe dyes SYBR Gold dyes

Real-time RT-PCR

Quantify gene expression with speed and accuracy.

Step 1: Isolate RNA
Isolate RNA using methods that preserve RNA integrity and expression profiles. RNA isolation kits Single Cell-to-CT Kit—For single cell analysis. Cells-to-CT kits—Rapid, simple protocol for RT-PCR sample prep without RNA extraction. TRIzol Reagent—Prepare high-quality, intact RNA from any biological material. mirVana miRNA isolation kits—Rapid isolation of small RNAs.
Step 2: Design and optimize primers
Not required for Applied Biosystems TaqMan Assays—These assays have passed our industry leading bioinformatics design criteria. Primer Express—For SYBR primer design
Step 3: Reverse transcription
Convert your RNA into cDNA, over a wide range of RNA concentrations. SuperScript IV VILO Master Mix—Two-step master mix for RT-qPCR applications - enabling earlier Ct values and improved data reproducibility, even with inhibitor-containing RNA samples. Other SuperScript reverse transcriptase formats
Step 4: Amplify cDNA
TaqMan real-time assays—Industry-leading, designed using our validated bioinformatics pipeline, and run with the same PCR protocol, eliminating the need for primer design or PCR optimization. Find the right assay for your research. Find assay now › Single Tube TaqMan Gene Expression Assays Single Tube Custom TaqMan Gene Expression Assays TaqMan Gene Expression Array Cards and Plates TaqMan OpenArray Plates TaqMan Real-Time PCR Master Mixes SYBR Green Master Mixes—Amplify your cDNA using Applied Biosystems SYBR Green master mix & custom primers. Custom Primers and Primer Express Software—Design and order custom primers for SYBR Green detection.
Step 5: Run qPCR
Instruments for real time PCR. Compare instruments › QuantStudio 3 Real-Time PCR System—Simple to use for all levels of experience QuantStudio 5 Real-Time PCR System—Easy controls for optimized experiments QuantStudio 6 Flex Real-Time PCR System—High flexibility and intuitive design QuantStudio 7 Flex Real-Time PCR System—High versatility and intuitive design QuantStudio 12k Flex Real-Time PCR System—Maximum throughput, flexibility, and scalability
Step 6: Analyze
Process and interpret qPCR data with intuitive software data analysis tools for a range of applications. Applied Biosystems qPCR analysis modules ExpressionSuite software

Next-generation Sequencing (NGS)

Confirm thousands of biomarkers quickly and reproducibly.

Need help with your project? Request a quote ›

Step 1: Select targets	Step 2: Construct library and/or prepare template
Oncomine TCR Beta-LR Assay Oncomine TCR Beta-SR Assay, DNA Oncomine TCR Beta-SR Assay, RNA Oncomine Immune Response Research Assay Oncomine Tumor Mutation Load Assay Oncomine assays for immuno-oncology and liquid biopsy research Ion AmpliSeq Designer tool for custom designs Learn more about Ion AmpliSeq panels ›	Ion Chef System Ion AmpliSeq kit for Chef DL8
Step 3: Sequence	Step 4: Analyze data
Ion GeneStudio S5 systems	Torrent Suite Software Ion Reporter Software Oncomine Reporter

Step 1: Select targets

Step 2: Construct library and/or prepare template

Learn more about Ion AmpliSeq panels ›

Step 3: Sequence

Step 4: Analyze data

Ion GeneStudio S5 systems

Cellular analysis

Study the impact of external and internal perturbations on cellular phenotypes and behavior.

Step 1: Observe	Step 2: Count
Click-iT colorimetric IHC detection kits Invitrogen primary and secondary antibodies for IHC Nunc cell culture plastics Gibco cell culture media	Countess II Automated Cell Counter Countess II FL Automated Cell Counter Countess II FL reusable slides Countess II disposable slides EVOS LED light cubes Invitrogen LIVE/DEAD reagents
Step 3: Visualize	Step 4: Analyze
EVOS M5000 Imaging System EVOS M7000 Imaging System with optional Celleste Image Analysis Software EVOS Onstage Incubator Invitrogen primary and secondary antibodies for IHC Alexa Fluor and Alexa Fluor Plus antibodies Invitrogen fluorescent reagents Nunc microplates for cell-based assays	Microplate reading: Varioskan LUX Multimode Microplate Reader Thermo Scientific microplate readers Invitrogen microplate assays Flow cytometry: Attune NxT Flow Cytometer Click-iT EdU assays CellTrace cell proliferation kits Vybrant DyeCycle stains FIX & PERM Cell Permeabilization Kit Alexa Fluor, and Qdot antibodies PrimeFlow RNA assays Quantitative imaging: CellInsight CX5, CX7, and CX7 LZR HCS/HCA platforms HCS CellMask, NuclearMask, and CellTrace stains Alexa Fluor Plus secondary antibodies ViewRNA Cell Plus Assay

Step 1: Observe

Step 2: Count

Step 3: Visualize

Step 4: Analyze

Microplate reading:

Flow cytometry:

Quantitative imaging:

Protein analysis

Analyze the identity, function, and level of expression of key proteins.

Step 1: Isolation and cleanup	Step 2: Separate
Pierce cell lysis solutions Pierce reagents, LC-MS grade Pierce protein concentrators POROS chromatography and purification resins Slide-A-Lyzer dialysis products Zeba desalting columns KingFisher purification systems for DNA, RNA, and protein purification Dynabeads magnetic beads for immunoprecipitation	Novex, WedgeWell, Bolt, and NuPAGE precast gels SureCast Gel Handcast System PageRuler and Spectra protein ladders Invitrogen Mini Gel Tank
Step 3: Transfer	Step 4: Detect
Invitrogen and Thermo Scientific Pierce transfer buffers Invitrogen nitrocellulose membranes Invitrogen PVDF membranes iBlot 2 Blotting System Power Blotter	iBind western devices Invitrogen primary antibodies and HRP secondary antibodies Alexa Fluor and Alexa Fluor Plus secondary antibodies SuperSignal substrates iBright Imaging Systems Invitrogen ProcartaPlex multiplex immunoassay kits
Step 5: Quantify	Step 6: Modify
Invitrogen ProQuantum high-sensitivity immunoassay kits Invitrogen ready-to-use ELISA kits Thermo Scientific microplate readers Invitrogen ProcartaPlex multiplex immunoassay kits Pierce Rapid Gold BCA and Coomassie protein assays Qubit fluorometers Thermo Scientific TMT isobaric labeling, proteases, peptide enrichment, calibration solutions, and standards Thermo Scientific SILAC reagents	Pierce crosslinking, labeling, and bioconjugation reagents

Targeted genetic analysis

Targeted genetic analysis

Confirm and quantify genetic changes for a wide range of selected DNA targets that are key to your research.

Genomic or viral DNA isolation

Isolate the highest-quality genomic or viral DNA from a range of sample types for use in all common molecular biology applications.

Fluorometric DNA quantification

Qubit 4 Fluorometer—single sample per run
Varioskan LUX Multimode Microplate Reader—up 1536 samples per run
Varioskan ALF Multimode Microplate Reader—up to 384 samples per run
Quant-iT DNA Quantification kits—multiple samples

UV-Vis Spectrophotometric DNA quantification

NanoDrop One/OneC Microvolume UV-Vis Spectrophotometer with Wi-Fi—single sample per run
Multiskan SkyHigh Microplate Spectrophotometer with µDrop plate for microvolume analysis—up to 16 microvolume samples or up to 384 samples per run
Varioskan ALF Multimode Microplate Reader with µDrop plate for microvolume analysis— up to 16 microvolume samples or up to 384 samples per run
Varioskan LUX Multimode Microplate Reader with µDrop plate for microvolume analysis—up to 16 microvolume samples or up to 384 samples per run

Endpoint PCR

Amplify DNA sequences for subsequent molecular biology applications.

Step 1: Amplify target DNA	Step 2: Analyze
SimpliAmp, Veriti, and ProFlex thermal cyclers MicroAmp plastic consumables Invitrogen custom DNA oligos Platinum Taq DNA Polymerase and Platinum SuperFi DNA Polymerase	E-Gel Power Snap Electrophoresis System E-Gel CloneWell II, SizeSelect II, and EX agarose Gels TrackIt ladders UltraPure Agarose SYBR Safe dyes

Real-time PCR

Accurately analyze specific DNA targets.

Step 1: Prepare samples
We provide multiple solutions to isolate and purify DNA. Kits for genomic DNA extraction—Explore our broad range of kits for purifying genomic DNA from a variety of samples including tissue, cells, blood, serum, plants, forensic samples, and more. DNA Extract All Reagents Kit—Obtain PCR-ready DNA from a wide variety of sample types, ranging from blood to buccal swabs to plant tissues in five minutes.
Step 2: Select assays
We offer a variety of TaqMan Genotyping Assays for a number of real-time PCR analysis applications. TaqMan SNP Genotyping Assays—The Predesigned TaqMan SNP Genotyping Assays collection includes millions of genome‑wide human assays, including common 1,000 Genome SNPs, HapMap SNPs, and coding SNP assay. Find assay › Custom TaqMan SNP Genotyping Assays—Create custom assays by submitting confidential target sequences to our secure assay design pipeline. Design assay › TaqMan Drug Metabolism Assays—A comprehensive collection of inventoried assays optimized for genotyping SNPs, insertions and deletions (indels), and multi‑nucleotide polymorphisms (MNPs) in drug metabolism-related genes.
Step 3: Set up reactions
Prepare a reaction plate using TaqMan Assays and TaqMan master mix, along with your DNA samples. No additional reagents are needed. TaqPath ProAmp Master Mix—Delivers accurate genotyping results, even from samples containing PCR inhibitors. Includes ROX passive reference dye.
Step 4: Run PCR
Amplify DNA and achieve allelic discrimination by running the PCR reaction on an Applied Biosystems real-time PCR system and/or thermal cycler. To better evaluate the accuracy of genotype calls, perform real-time time PCR and obtain genotyping data for every cycle. Or for higher throughput, run PCR on a thermal cycler and then perform an endpoint read on a real-time instrument. Thermocycler instruments ProFlex PCR System—The ProFlex PCR System’s interchangeable block formats allow you to maximize your throughput or run independent experiments concurrently. SimpliAmp Thermal Cycler—The SimpliAmp Thermal Cycler is an easy-to use, compact, and accurate thermal cycler designed to fit every lab’s essential PCR workflow. Real-time PCR instruments Compare instruments › QuantStudio 3 Real-Time PCR System—Simple to use for all levels of experience QuantStudio 5 Real-Time PCR System—Easy controls for optimized experiments QuantStudio 6 Flex Real-Time PCR System—High flexibility and intuitive design QuantStudio 7 Flex Real-Time PCR System—High versatility and intuitive design QuantStudio 12k Flex Real-Time PCR System—Maximum throughput, flexibility, and scalability
Step 5: Analyze data
Process and interpret qPCR data with intuitive software data analysis tools for a range of applications. Applied Biosystems qPCR Analysis Modules—Our cloud-based applications can bring together multiple data sets, with analysis speeds up to 10 times faster than our desktop software. The Genotyping app includes improved visuals and integrated traces of allelic discrimination plots to allow thorough QC of SNP assays to accurately reflect the true signals versus background noise. TaqMan Genotyper Software—TaqMan Genotyper Software is for use with TaqMan SNP Genotyping Assays (Predesigned, Custom & DME) in combination with 48-, 96-, and 384-well microplates or OpenArray Genotyping Plates, and offers a state-of-the-art genotype-calling algorithm, an intuitive user interface, and enhanced multi-plate analysis features.

Sanger sequencing

Verify DNA sequence rapidly using proven DNA sequencing technology.

Step 1: PCR amplification	Step 2: PCR clean-up
AmpliTaq DNA Polymerase Primer Designer Tool for PCR and Sanger sequencing	ExoSAP-IT Express reagent
Step 3: Cycle sequencing	Step 4: Sequencing clean-up
BigDye Terminator cycle and direct cycle sequencing kits	BigDye XTerminator Purification Kit
Step 5: Sequence	Step 6: Analyze data
SeqStudio Genetic Analyzer Applied Biosystems 3500 Genetic Analyzer	Applied Biosystems Sanger Analysis modules Minor Variant Finder Software

Next-generation Sequencing (NGS)

Sequence hundreds to thousands of targets at high depth.

Need help with your project? Request a quote ›

Step 1: Select targets	Step 2: Construct library and/or prepare template
Oncomine TCR Beta-LR Assay Oncomine TCR Beta-SR Assay, DNA Oncomine TCR Beta-SR Assay, RNA Oncomine Immune Response Research Assay Oncomine Tumor Mutation Load Assay Oncomine assays for immuno-oncology and liquid biopsy research Ion AmpliSeq Designer tool for custom designs Learn more about Ion AmpliSeq panels ›	Ion Chef System Ion AmpliSeq kit for Chef DL8
Step 3: Sequence	Step 4: Analyze data
Ion GeneStudio S5 systems	Torrent Suite Software Ion Reporter Software Oncomine Reporter

Step 1: Select targets

Step 2: Construct library and/or prepare template

Learn more about Ion AmpliSeq panels ›

Step 3: Sequence

Step 4: Analyze data

Ion GeneStudio S5 systems

Targeted gene expression analysis

Targeted gene expression analysis

Confirm and quantify RNA targets at the gene, exon, or noncoding RNA level.

RNA Isolation

Isolate the highest-quality cellular RNA, viral RNA, or miRNA from a range of sample types for direct use in all common molecular biology applications

Fluorometric DNA quantification

Qubit 4 Fluorometer—single sample per run
Varioskan LUX Multimode Microplate Reader—up 1536 samples per run
Varioskan ALF Multimode Microplate Reader—up to 384 samples per run
Quant-iT DNA Quantification kits—multiple samples

UV-Vis Spectrophotometric DNA quantification

NanoDrop One/OneC Microvolume UV-Vis Spectrophotometer with Wi-Fi—single sample per run
Multiskan SkyHigh Microplate Spectrophotometer with µDrop plate for microvolume analysis—up to 16 microvolume samples or up to 384 samples per run
Varioskan ALF Multimode Microplate Reader with µDrop plate for microvolume analysis— up to 16 microvolume samples or up to 384 samples per run
Varioskan LUX Multimode Microplate Reader with µDrop plate for microvolume analysis—up to 16 microvolume samples or up to 384 samples per run

RT- PCR

Confirm and compare gene expression for small sample numbers by RT-PCR

Step 1: Synthesize and amplify cDNA	Step 2: Analyze data
Invitrogen custom DNA oligos Invitrogen Value oligos SuperScript IV One-Step RT-PCR System SuperScript IV One-Step RT-PCR System with ezDNase Enzyme MicroAmp plastic consumables SimpliAmp, Veriti, and ProFlex thermal cyclers	E-Gel Power Snap Electrophoresis System E-Gel CloneWell II, SizeSelect II, and EX agarose gels TrackIt ladders UltraPure Agarose SYBR Safe dyes SYBR Gold dyes

Real-time RT-PCR

Quantify gene expression with speed and accuracy.

Step 1: Isolate RNA
Isolate RNA using methods that preserve RNA integrity and expression profiles. RNA isolation kits Single Cell-to-CT Kit—For single cell analysis. Cells-to-CT kits—Rapid, simple protocol for RT-PCR sample prep without RNA extraction. TRIzol Reagent—Prepare high-quality, intact RNA from any biological material. mirVana miRNA isolation kits—Rapid isolation of small RNAs.
Step 2: Design and optimize primers
Not required for Applied Biosystems TaqMan Assays—These assays have passed our industry leading bioinformatics design criteria. Primer Express—For SYBR primer design
Step 3: Reverse transcription
Convert your RNA into cDNA, over a wide range of RNA concentrations. SuperScript IV VILO Master Mix—Two-step master mix for RT-qPCR applications - enabling earlier Ct values and improved data reproducibility, even with inhibitor-containing RNA samples. Other SuperScript reverse transcriptase formats
Step 4: Amplify cDNA
TaqMan real-time assays—Industry-leading, designed using our validated bioinformatics pipeline, and run with the same PCR protocol, eliminating the need for primer design or PCR optimization. Find the right assay for your research. Find assay now › Single Tube TaqMan Gene Expression Assays Single Tube Custom TaqMan Gene Expression Assays TaqMan Gene Expression Array Cards and Plates TaqMan OpenArray Plates TaqMan Real-Time PCR Master Mixes SYBR Green Master Mixes—Amplify your cDNA using Applied Biosystems SYBR Green master mix & custom primers. Custom Primers and Primer Express Software—Design and order custom primers for SYBR Green detection.
Step 5: Run qPCR
Instruments for real time PCR. Compare instruments › QuantStudio 3 Real-Time PCR System—Simple to use for all levels of experience QuantStudio 5 Real-Time PCR System—Easy controls for optimized experiments QuantStudio 6 Flex Real-Time PCR System—High flexibility and intuitive design QuantStudio 7 Flex Real-Time PCR System—High versatility and intuitive design QuantStudio 12k Flex Real-Time PCR System—Maximum throughput, flexibility, and scalability
Step 6: Analyze
Process and interpret qPCR data with intuitive software data analysis tools for a range of applications. Applied Biosystems qPCR analysis modules ExpressionSuite software

Next-generation Sequencing (NGS)

Confirm thousands of biomarkers quickly and reproducibly.

Need help with your project? Request a quote ›

Step 1: Select targets	Step 2: Construct library and/or prepare template
Oncomine TCR Beta-LR Assay Oncomine TCR Beta-SR Assay, DNA Oncomine TCR Beta-SR Assay, RNA Oncomine Immune Response Research Assay Oncomine Tumor Mutation Load Assay Oncomine assays for immuno-oncology and liquid biopsy research Ion AmpliSeq Designer tool for custom designs Learn more about Ion AmpliSeq panels ›	Ion Chef System Ion AmpliSeq kit for Chef DL8
Step 3: Sequence	Step 4: Analyze data
Ion GeneStudio S5 systems	Torrent Suite Software Ion Reporter Software Oncomine Reporter

Step 1: Select targets

Step 2: Construct library and/or prepare template

Learn more about Ion AmpliSeq panels ›

Step 3: Sequence

Step 4: Analyze data

Ion GeneStudio S5 systems

Cellular analysis

Cellular analysis

Study the impact of external and internal perturbations on cellular phenotypes and behavior.

Step 1: Observe	Step 2: Count
Click-iT colorimetric IHC detection kits Invitrogen primary and secondary antibodies for IHC Nunc cell culture plastics Gibco cell culture media	Countess II Automated Cell Counter Countess II FL Automated Cell Counter Countess II FL reusable slides Countess II disposable slides EVOS LED light cubes Invitrogen LIVE/DEAD reagents
Step 3: Visualize	Step 4: Analyze
EVOS M5000 Imaging System EVOS M7000 Imaging System with optional Celleste Image Analysis Software EVOS Onstage Incubator Invitrogen primary and secondary antibodies for IHC Alexa Fluor and Alexa Fluor Plus antibodies Invitrogen fluorescent reagents Nunc microplates for cell-based assays	Microplate reading: Varioskan LUX Multimode Microplate Reader Thermo Scientific microplate readers Invitrogen microplate assays Flow cytometry: Attune NxT Flow Cytometer Click-iT EdU assays CellTrace cell proliferation kits Vybrant DyeCycle stains FIX & PERM Cell Permeabilization Kit Alexa Fluor, and Qdot antibodies PrimeFlow RNA assays Quantitative imaging: CellInsight CX5, CX7, and CX7 LZR HCS/HCA platforms HCS CellMask, NuclearMask, and CellTrace stains Alexa Fluor Plus secondary antibodies ViewRNA Cell Plus Assay

Step 1: Observe

Step 2: Count

Step 3: Visualize

Step 4: Analyze

Microplate reading:

Flow cytometry:

Quantitative imaging:

Protein analysis

Protein analysis

Analyze the identity, function, and level of expression of key proteins.

Step 1: Isolation and cleanup	Step 2: Separate
Pierce cell lysis solutions Pierce reagents, LC-MS grade Pierce protein concentrators POROS chromatography and purification resins Slide-A-Lyzer dialysis products Zeba desalting columns KingFisher purification systems for DNA, RNA, and protein purification Dynabeads magnetic beads for immunoprecipitation	Novex, WedgeWell, Bolt, and NuPAGE precast gels SureCast Gel Handcast System PageRuler and Spectra protein ladders Invitrogen Mini Gel Tank
Step 3: Transfer	Step 4: Detect
Invitrogen and Thermo Scientific Pierce transfer buffers Invitrogen nitrocellulose membranes Invitrogen PVDF membranes iBlot 2 Blotting System Power Blotter	iBind western devices Invitrogen primary antibodies and HRP secondary antibodies Alexa Fluor and Alexa Fluor Plus secondary antibodies SuperSignal substrates iBright Imaging Systems Invitrogen ProcartaPlex multiplex immunoassay kits
Step 5: Quantify	Step 6: Modify
Invitrogen ProQuantum high-sensitivity immunoassay kits Invitrogen ready-to-use ELISA kits Thermo Scientific microplate readers Invitrogen ProcartaPlex multiplex immunoassay kits Pierce Rapid Gold BCA and Coomassie protein assays Qubit fluorometers Thermo Scientific TMT isobaric labeling, proteases, peptide enrichment, calibration solutions, and standards Thermo Scientific SILAC reagents	Pierce crosslinking, labeling, and bioconjugation reagents

Resources

Support

Services and Support

For Research Use Only. Not for use in diagnostic procedures.

Immuno-Oncology Characterization and Verification Tools

Explore our characterization and verification solutions

Targeted genetic analysis

Genomic or viral DNA isolation

Fluorometric DNA quantification

UV-Vis Spectrophotometric DNA quantification

Thermocycler instruments

Real-time PCR instruments Compare instruments ›

Targeted gene expression analysis

RNA Isolation

Fluorometric DNA quantification

UV-Vis Spectrophotometric DNA quantification

Instruments for real time PCR. Compare instruments ›

Cellular analysis

Microplate reading:

Flow cytometry:

Quantitative imaging:

Protein analysis

Targeted genetic analysis

Genomic or viral DNA isolation

Fluorometric DNA quantification

UV-Vis Spectrophotometric DNA quantification

Thermocycler instruments

Real-time PCR instruments Compare instruments ›

Targeted gene expression analysis

RNA Isolation

Fluorometric DNA quantification

UV-Vis Spectrophotometric DNA quantification

Instruments for real time PCR. Compare instruments ›

Cellular analysis

Microplate reading:

Flow cytometry:

Quantitative imaging:

Protein analysis

Resources

Support