Protein Isolation and Purification Information | Thermo Fisher Scientific

Learn about methods and technologies for efficient cell lysis, protein extraction and fractionation, targeted inhibition of unwanted protease and phosphatase activity, and convenient devices and high performance resins for the purification and clean-up of proteins and antibodies for downstream applications.

Cell lysis
Protein extraction
Protease & phosphatase inhibitors
Desalting & Dialysis
Sample clean-up
Affinity purification
Resins, beads, supports
Ligand immobilization
Immunoprecipitation (IP)

Technical handbooks
Protein methods articles
Product application notes
Support center resources
Protocols
Product selection guides

Protein isolation and purification features

Methods article

An Overview of Affinity Purification

Affinity chromatography or purification is a powerful method whereby a protein of interest is purified from other components in a crude cell lysate or other sample by virtue of its specific binding properties to an immobilized ligand.

Methods article

Overview of Immunoprecipitation (IP) Technique

What is immunoprecipitation? IP is the small-scale affinity purification of antigens using a specific antibody that is immobilized to a solid support such as magnetic particles or agarose resin.

Protein isolation and purification learning resources

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Type	Title
Handbook	Protein Research Handbook (2016)
Methods article	The Pierce Protein Methods Library
Methods article	An Overview of Cell Lysis and Protein Extraction
Methods article	Traditional Methods of Cell Lysis
Methods article	Detergents for Cell Lysis and Protein Extraction
Methods article	Cell Lysis Solutions
Methods article	Cell Fractionation and Organelle Isolation
Methods article	Protease and Phosphatase Inhibitors
Methods article	An Overview of Affinity Purification
Methods article	Covalent Immobilization of Affinity Ligands
Methods article	GST-tagged Proteins–Production and Purification
Methods article	His-tagged Proteins–Production and Purification
Methods article	An Overview of the Immunoprecipitation (IP) Technique
Methods article	An Overview of Buffer Exchange
Methods article	Dialysis Methods for Protein Research
Methods article	Desalting and Gel Filtration Chromatography
Selection guide	Cell Lysis and Fractionation (Selection Guides)
Selection guide	Dialysis Products Selection Guide
Selection guide	Desalting Products Selection Guide
Selection guide	Protein Purification (Selection Guides)
Selection guide	Immunoprecipitation (Selection Guide)
Selection guide	Protein Purification and Analytics Interactive Decision Tool
Selection guide	Bioprocessing and Manufacturing Protein Analytics and Production Tool
Application note	Product Application Notes
Application note	An improved method for highly efficient isolation of primary mouse embryonic fibroblasts. Easily isolate and culture primary mouse embryonic fibroblasts (MEF).
Application note	One-hour procedure to isolate primary cardiomyocytes from neonatal mouse and rat hearts. Easily isolate and culture highly viable, functional primary cardiomyocytes.
Application note	Benchmarking experiments with Zeba Spin Desalting Columns. Protein recovery performance comparisons of Thermo Scientific Zeba Spin Desalting Columns to other commercially available desalting resins and columns.
Application note	Calculate the number of immobilized proteins per bead of agarose affinity supports. A consideration of relative sizes and dimensions of agarose resin beads, antibodies, proteins, chemical modification groups, and affinity ligands.
Application note	Improved, all-in-one B-PER Reagent for bacterial protein extraction. B-PER Complete Reagent provides gentle, efficient, and cost effective protein extraction from E. coli and other bacterial cells.
Application note	An efficient method for the isolation of highly functional primary neurons. Easily and reproducibly isolate and culture primary neurons.
Application note	Efficient mammalian membrane protein extraction. Fast and simple enrichment of integral membrane proteins and membrane-associated proteins.
Application note	Performance characterization of Pierce Glutathione Superflow Agarose. Purity, dynamic binding capacity, reusability and chemical compatibility properties of a glutathione resin for FPLC purification of GST tagged fusion proteins.
Application note	Performance characterization of HisPur Ni-NTA Superflow Agarose. Purity, dynamic binding capacity, reusability and chemical compatibility properties of a nickel-charged IMAC resin for FPLC purification of His-tagged proteins.
Application note	Performance characterization of HisPur Cobalt Superflow Agarose. Purity, dynamic binding capacity, reusability and chemical compatibility properties of a cobalt-charged IMAC resin.
Application note	Separation characteristics of dialysis membranes. Molecular weight cut-off (MWCO) specifications and rates of buffer exchange with Slide-A-Lyzer Dialysis Devices and Snakeskin Dialysis Tubing.
Application note	Chromatography cartridges for desalting and affinity purification. Data using the cartridge format for automated liquid chromatography (LC) systems.
Application note	Phosphoprotein enrichment from cell and tissue samples. Isolate phosphorylated proteins with an easy-to-use spin format
Application note	Eliminate endotoxins from protein and antibody samples. A highly specific affinity resin enables endotoxin removal from various protein sources, sizes and charges.
Application note	Removing endotoxins using a spin-column format. Fast and effective removal of endotoxins from protein samples.
Application note	Fractionate subcellular proteins from tissue samples. Obtain proteins from five cellular components using a tissue-optimized kit.
Application note	Method to isolate functional synaptosomes. Enrich neuronal synaptic proteins while maintaining phosphoprotein integrity.
Application note	Complete protein protection from proteases and phosphatases. Protease, phosphatase and combination inhibitor tablets provide a convenient format to protect proteins from degradation and dephosphorylation.
Application note	Optimized protein extraction reagent for neuronal tissue and primary cells. Obtain high yields of functional neuronal protein from all neuronal cell compartments.
Application note	Using antibody-binding proteins for antibody purification. Choose a format based on the specific application and scale.
Application note	New dimensions for low-volume sample dialysis. Self-contained devices give you the solution for high-protein recovery.
Application note	Perform IP and co-IP experiments with high yield and low background. New Protein A/G magnetic beads enable manual and automated applications.
Protocol	Protein Isolation Protocols (for Dynabeads Magnetic Beads)

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For Research Use Only. Not for use in diagnostic procedures.