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Insect Cell Culture Support
Explore our “Getting Started” and “Troubleshooting” sections for solutions to top inquiries and common problems. Browse through our "Guides and Tools" section to access a comprehensive portfolio of product-related support resources.
Getting Started
- What are the important parameters for routine culture and maintenance of insect cells?
- What is the best way to plate insect cells?
- How many cells do you recommend to use for “seeding” a flask at 50% confluence for insect cells?
- Do you offer serum free insect media?
- What antimicrobials can be used in insect culture and at what concentration?
Troubleshooting
- I want to split my Sf9 cells but don’t know how to do this. What do you recommend?
- I have Sf9 cells in suspension culture. The cells look really bad: 50% dead three days after reviving. Are they contaminated?
- What are small particles that appear after a few days when growing Sf9 cells? I am not using Gibco™ Fungizone™ reagent, but I am using gentamicin. Are they yeast?
- My High Five™ cells are in log-phase growth in Grace’s Insect Medium plus serum. Why are the High Five™ cells forming large, free-floating clumps with very few cells attached?
- Are spindle shaped Sf9 cells bad? How do I get rid of them?
Guides and Tools
Brochure: Enhance Serum-free Insect Cell Culture with Gibco™ Media
Brochure: Insect Cell Culture
Manual: Growth and Maintenance of Insect Cell Lines
Search our database for Product Documents: Manuals, Media Formulations, Citations and References
Search our database for Quality and Safety Documents: MSDS, COA
- Overview: Serum-Free Insect Media
- Overview: Sf-900™ III SFM Media
- Learning Center: Cell Culture & Transfection
- Cell Culture & Transfection Resource Library (useful technical, application, and other learning resources including application notes and methods)
- Guide: Gibco™ Cell Culture Basics Handbook
- Selection: Which Insect Media is Right for You
- Videos: Gibco Cell Culture Basics Video Collection
- Browse our site for: Training, Events and Webinars
Gibco™ Custom Media Configurator: Adjust many of our Gibco™ media formulations to create a custom cell culture medium that's just right for you.
- Cell Imaging Guide with Protocols (iPad™ device version): Find fluorescent dyes, reagents, and protocols for cell biology related fluorescence microscopy applications.
- Fluorescence SpectraViewer: Plot and compare spectra, check the spectral compatibility for fluorophores and email the configuration to yourself in a clear printable format.
- Gibco™ Cell Culture Companion: Capture and store vital experiment information on your iPhone™ or iPad™ device in the lab as you count cells, perform cell culture calculations, and generate growth curves. And when you're finished, you can export all data files to your Mac™ or PC.
- 3D Cell (iPad™device version): Learn about the cell and all its structures, rotate the cell 360 degrees, watch live-cell videos, and virtually stain your own cell with Invitrogen™ Molecular Probes™ dyes.
Custom Services
Custom Cell Culture Media Manufacturing Services
Every cell culture project is unique. We are committed to providing you with products and services customized to your research and production needs. Presenting the Gibco™ quality media you know and trust; customized to your specifications.
Gibco™ Bioprocess Services Project Solutions
The Bioproduction Process Development Team provides in-the-field scientific and business support for Gibco™ cell line and media products as well as Gibco™ PD-Direct™ Services. The unique combination of process science consultants and business development managers work globally to provide product strategies and customized solutions.
Gibco™ Custom Media Configurator
Adjust many of our Gibco™ media formulations to create a custom cell culture medium that's just right for you. Add and remove components, customize packaging options and choose QC tests. Customize Gibco™ cell culture media products from 1 L to 10,000 L.
For Research Use Only. Not for use in diagnostic procedures.