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Drug Discovery Assays Support
Explore our “Getting Started” and “Troubleshooting” sections for solutions to top inquiries and common problems related to your drug discovery and development experiments. Browse through our “Guides and Tools” section to access a comprehensive portfolio of product-related support resources.
Getting started
- What products and services do you offer for drug discovery research?
- What methods of detection do you offer for the various druggable target classes?
- What is the first consideration for getting started with a ratiometric fluorescent assay?
- I think that many of my test compounds may not be active, what is the best approach to screening?
- What sort of dilution series is recommended to determine an EC50 or an IC50?
Troubleshooting
- I have no assay window in my drug discovery assay. Why is this?
- I have no assay window in my TR-FRET Assay. What is the reason?
- My EC50 (or IC50) results are different from a colleague’s in another lab. Why?
- Is a 30-fold window better than a 10-fold window in a drug discovery assay?
- My cells were not viable upon thaw. What happened?
Guides and Tools
Kinase activity assays
- Application Note: Method to Test Microplate Readers for LanthaScreen Tb Assays
- Application Note: Method to Test Microplate Reader for LanthaScreen Eu Assays
- User Guide: SelectScreen Biochemical Kinase Profiling Service - Adapta Assay Screening Protocol and Assay Conditions
- User Guide: SelectScreen Biochemical Kinase Profiling Service - Z’-LYTE Assay Screening Protocol and Assay Conditions
Kinase binding assays
- Application Note: Measure Inhibitor Binding to Non-Activated Kinases - LanthaScreen Eu Kinase Binding Assay
- Application Note: Simple Off-Rate Measurements - LanthaScreen Eu Kinase Binding Assay
- Application Note: Method to Test Microplate Readers for LanthaScreen Eu Assays
- Technical Note: LanthaScreen Eu Kinase Binding Assay for Type III Kinase inhibitors
- User Guide: SelectScreen Biochemical Kinase Profiling Service - LanthaScreen Eu Kinase Binding Assay Screening Protocol and Assay Conditions
Nuclear receptor assays
- Poster: Tools for the Discovery and Characterization of Glucocorticoid Receptor (GR) Modulators - A Comparison of Binding, Coregulator Interaction, and Transactivation Assays
- Poster: Tools for the Discovery and Characterization of Glucocorticoid Receptor (GR) Modulators
- Poster: Fluorescence-Based Biochemical Assays for the Study of Pregnane X Receptor and Constitutive Androstane Receptor
- User Guide: SelectScreen Biochemical Nuclear Receptor Profiling Service - LanthaScreen TR-FRET Competitive Binding Assay Screening Protocol and Assay Conditions
- User Guide: SelectScreen Biochemical Nuclear Receptor Profiling Service - LanthaScreen TR-FRET Coregulator Protocol and Assay Conditions
- User Guide: SelectScreen Cell-Based Nuclear Receptor Profiling Service - SelectScreen GeneBLAzer Cellular Assay Screening Protocol and Assay Conditions
GPCR assays
- Brochure: SelectScreen Cell-Based GPCR Profiling Service - Couple your GPCR Profiling Needs with Our Expertise
- Poster: SelectScreen Cell-Based GPCR Profiling Service - Rapid Profiling of Compounds with Functional Assays in Agonist and Antagonist Mode
- Poster: SelectScreen Cell-Based GPCR Profiling Service - Compound Screening with the k Opioid Receptor Utilizing Different Cell-Based Assay Platforms
- User Guide: SelectScreen Cell-Based GPCR Profiling Service - Screening Protocol and Assay Conditions
Pathway analysis assays
- Poster: LanthaScreen Cellular STAT 1/3/5 Assays for High-Throughput Screening of JAK Inhibitors
- Poster: Cellular Assays for Interrogating the PI3K/AKT/mTOR Pathway
- Poster: High-Throughput CellSensor Reporter Assays for Multi-Pathway Analysis
- Poster: Multi-Pathway Profiling Approach to Compound Validation - Utilizing SelectScreen Cell-Based Pathway Screening to Characterize Pathway Selectivity for Commonly Used Protein Kinase Inhibitors
- Poster: Use of BacMam Technology to Express Full-Length LRRK2 and to Profile Inhibitors Using Cell-Based and Biochemical Assays
- User Guide: SelectScreen Cell-Based Pathway Profiling Service - CellSensor Cell Lines Screening Protocol and Assay Conditions
- User Guide: LanthaScreen Cellular Profiling Service - LanthaScreen Cellular Assay Screening Protocol and Assay Conditions
Ion channel assays
- FAQ: Predictor hERG Fluorescence Polarization Assay
- Poster: Performance of the Predictor Fluorescence Polarization Assay - A Tool for High-Throughput Screening of hERG Channel Affinity
- User Guide: Predictor hERG Fluorescence Polarization Assay
Cytochrome P450 activity, baculosome, and Vivid kits
- Brochure: Highly Miniaturized Formats for In Vitro Drug Metabolism Assays Using Vivid Fluorescent Substrates and Recombinant Human Cytochrome P450 Enzymes
- Poster: Miniaturization and Automation of Cytochrome P450 Inhibition Assays
- User Guide: Vivid CYP450 Screening Kits
- User Guide: SelectScreen Biochemical P450 Profiling Service - Screening Protocol and Assay Conditions
Phosphatase activity assays
- Application Note: Phosphate Sensor for Sensitive Detection of ATPase Activity
- Application Note: Phosphate Sensor for Detection of GTPase Activity
- Application Note: Rapid, Sensitive Detection of Protein Phosphatase Activity
- Application Note: Simple, Sensitive Detection of Protein Phosphodiesterase Activity
Search our database for Product Documents:
Manuals,
Product Literature,
Citations and References
Search our database for Quality and Safety Documents: MSDS, COA
Search our database for Quality and Safety Documents: MSDS, COA
General
- Overview: Drug Discovery & Development
- Overview: Drug Discovery & Development Quick Links
- Overview: Drug Discovery Assays
- Overview: Instrument Compatibility Portal
- Overview: Validated Cell-Based Assays for Discovery and Screening
- Overview: SelectScreen Screening and Profiling Services
Kinase biology
- Overview: Kinase Biology
- Overview: Kinase Proteins
- Overview: Biochemical Kinase Assays
- Overview: Kinases for Disease Research
- Overview: SelectScreen Kinase Profiling Services
- Selection: Kinases
- Selection: Mutant Kinases
- Selection: Kinase Inhibitors
Kinase activity assays
- Overview: Adapta Universal Kinase Assay and Substrates
- Overview: LanthaScreen Kinase Activity Assays
- Overview: Z’-LYTE Kinase Assay Kits
- Overview: Adapta Assay - Compatible Instruments and Setup Guides
- Overview: LanthaScreen Tb Assay - Compatible Instruments and Setup Guides
- Overview: LanthaScreen Eu Assay - Compatible Instruments and Setup Guides
- Overview: Z’-LYTE Assay - Compatible Instruments and Setup Guides
- Selection: Biochemical Kinase Assays
- Selection: Adapta Kinase Activity Assay Reactivity Table and Kinase-Specific Protocols
- Selection: LanthaScreen Kinase Activity Assay Reactivity Table and Kinase-Specific Protocols
- Selection: Z’-LYTE Kinase Activity Assay Reactivity Table and Kinase-Specific Protocols
Kinase binding assays
- Overview: LanthaScreen Eu Kinase Binding Assay
- Overview: LanthaScreen Eu Assay - Compatible Instruments and Setup Guides
- Selection: LanthaScreen Eu Kinase Binding Assay Reactivity Table and Kinase-Specific Protocols
Nuclear receptor assays
- Overview: Nuclear Receptor Biology
- Overview: Nuclear Receptor Biochemical Assays
- Overview: Nuclear Receptor Cell-Based Assays
- Overview: SelectScreen Nuclear Receptor Profiling Services
- Overview: Compatible Instruments and Setup Guides
- Selection: PolarScreen NR Competitive Binding Assays
- Selection: LanthaScreen Fluorescent Coregulator Peptides
- Selection: LanthaScreen TR-FRET Nuclear Receptor Coregulator Interaction Assays
- Selection: GeneBLAzer Cell-Based Assays for Nuclear Receptors
Fluorescence polarization (FP) assays
- Technical Note: Fluorescence Polarization
GPCR assays
- Overview: G-Protein Coupled Receptors (GPCR) Biology
- Overview: Cell-Based GPCR Reporter Assays
- Overview: Cell-Based Calcium Flux Second Messenger Assays
- Overview: SelectScreen Cell-Based GPCR Profiling Services
Pathway analysis assays
- Overview: Pathway Biology
- Overview: Cellular Pathway Analysis Assays
- Overview: The BacMam System
- Overview: GeneBLAzer BLA Reporter Assays - Compatible Instruments and Setup Guides
- Overview: Cellular LanthaScreen Assays - Compatible Instruments and Setup Guides
- Overview: SelectScreen Cell-Based Pathway Profiling Services
- Overview: Custom BacMam Production
- Publication: Cellular LanthaScreen and Beta-Lactamase Reporter Assays for High-Throughput Screening of JAK2 Inhibitors
- Publication: Multi-Pathway Cellular Analysis of Compound Selectivity
- Publication: High-Throughput Cellular Assays for Regulated Posttranslational Modifications
- Selection: LanthaScreen Cellular Assays
- Selection: LanthaScreen Cellular Assays Validation Table
- Selection: CellSensor Cell Lines and GeneBLAzer Beta-Lactamase Reporter Assays
- Selection: CellSensor Vectors
- Selection: CellSensor Cell Lines Validation Table
- Selection: BacMam-Enabled Cellular Assays
Ion channel assays
- Overview: Ion Channel Biology
- Overview: Cell-Based Ion Channel Assays
- Overview: Predictor Fluorescence Polarization Assay - Compatible Instruments and Setup Guides
- Overview: SelectScreen hERG Screening Service
- Publication: Development of the Predictor hERG Fluorescence Polarization Assay Using a Membrane Protein Enrichment Approach
Cytochrome P450 activity, baculosomes, and Vivid kits
- Overview: Cytochrome P450 Assays
- Overview: Baculosomes Plus P450 Proteins
- Overview: Vivid P450 Assay Reagents
- Overview: SelectScreen P450 Profiling Service
Phosphatase activity assays
- Selection: Phosphatase Assay Kits
- LanthaScreen Eu Kinase Binding Assay Calculator: Use this calculator to help determine the reagent amounts needed for a specific time.
- Drug Discovery Assay Maker: Make a high-throughput screening assay that fits your unique research needs.
- Fluorescence SpectraViewer: Plot and compare spectra, check spectral compatibility, and email the configuration to yourself or others in the lab.
- Fluorescence SpectraViewer Mobile App: An app to plot and compare spectra, check spectral compatibility, and email the configuration to yourself or others in the lab. This mobile version of SpectraViewer acts as an extension of our online Fluorescence SpectraViewer.
- DailyCalcs Science Calculator: An app designed to calculate molarity, dilution, molecular weight, and more. 8 Calculators, including a new Specific Productivity Calculator for use with fed-batch cell culture processes and a Transfection calculator you can use to simplify transfection experimental setup.
Custom Services
With dedicated specialists who listen to your needs, and a team of experts in screening, profiling and custom assay development, we can extend your research team by providing support before, during, and after your experiments.
Choose from the broadest collection of off-the-shelf target-based assays; or let us lend a hand by outsourcing your screening, profiling or custom assay projects to our team of Custom Biology scientists.
We offer stem cell researchers choices at every stage of their research—innovative tools that make it easier for you to “do it yourself” and a custom services offering that utilizes our team of stem cell experts to deliver your desired results. Choose the combination of tools and services that best fits your lab resources and expertise.
For Research Use Only. Not for use in diagnostic procedures.