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The Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance.
- View the Molecular Devices website
Before using LanthaScreen® Terbium and Europium assays
Test your plate reader set-up
These technical notes provide a method for verifying that a fluorescent plate reader is able to detect a change in time-resolved fluorescence energy transfer (TR-FRET) signal, confirming proper instrument set-up and a suitable response. The method is independent of any biological reaction or equilibrium and uses reagents that are on-hand for the LanthaScreen® assay.
SpectraMax® Paradigm® Microplate Detection Platform
|
Instrument Configuration:
| Assay Technology | Adapta® & LanthaScreen® Europium | GeneBLAzer® | LanthaScreen® Terbium | Predictor™ | Z'-LYTE® |
|---|---|---|---|---|---|
| Modality | TR-FRET | FRET (bottom) | TR-FRET | FP | FRET |
| Setup Guide |  | | | | |
| Application Note | - | - | - | - | - |
| Poster | - | - | - | - | - |
| Excitation Filter | 406/15 | 532/25 | |||
| Emission 1 Filter | 465/35 | 595/35 | |||
| Emission 2 Filter | 535/25 | 595/35 | |||
| Dichroic Mirror | 508 | ||||
| Optic Module | GeneBLAzer® | FP rhodamine |
SpectraMax® M5/M5e Microplate Reader
& FlexStation® 3 Benchtop Multi-Mode Microplate Reader
 |
|
Instrument Configuration:
| Assay Technology | Adapta® & LanthaScreen® Europium | GeneBLAzer® | LanthaScreen® Terbium | Predictor™ | Z'-LYTE® |
|---|---|---|---|---|---|
| Modality | TR-FRET | FRET (bottom) | TR-FRET | FP | FRET |
| Setup Guide |
|
|
| Reader not |
|
| Application Note | - | - | - | - | - |
| Poster | - | - | - | - | - |
| Excitation | 332/12 | 410/12 | 332/12 | 410/12 | |
| Emission 1 | 620/12 | 458/12 | 488/12 | 458/12 | |
| Emission 2 | 666/12 | 522/12 | 518/12 | 522/12 | |
| Dichroic Mirror | 590/630 | 435/495 | 420 | 435/495 |
FilterMax F5 Multi-Mode Microplate Reader
|
Instrument Configuration:
| Assay Technology | Adapta® & LanthaScreen® Europium | GeneBLAzer® | LanthaScreen® Terbium | Predictor™ | Z'-LYTE® |
|---|---|---|---|---|---|
| Modality | TR-FRET | FRET (bottom) | TR-FRET | FP | FRET |
| Setup Guide | Reader not compatible | | Reader not compatible | Reader not compatible | |
| Application Note | - | - | - | - | - |
| Poster | - | - | - | - | - |
| Excitation Filter | 405/30 | 405/30 | |||
| Emission 1 Filter | 465/35 | 465/35 | |||
| Emission 2 Filter | 535/25 | 535/25 | |||
| Dichroic Mirror |
Analyst® Multimode Reader
|
Instrument Configuration:
| Assay Technology | Adapta® & LanthaScreen® Europium | GeneBLAzer® | LanthaScreen® Terbium | Predictor™ | Z'-LYTE® |
|---|---|---|---|---|---|
| Modality | TR-FRET | FRET (bottom) | TR-FRET | FP | FRET |
| Setup Guide | - | - | | - | - |
| Application Note | - | - | - | - | - |
| Poster | - | - | - | - | - |
| Excitation Filter (25 mm) | 340/30 (PV00225) | 405/20 | 340/30 (PV00225) | 530/25 | 405/20 |
| Emission 1 Filter (25 mm) | 620/7.5 | 460/40 | 495/10 (PV00325) | 580/10 | 460/40 |
| Emission 2 Filter (25 mm) | 665/7.5 | 530/25 | 520/25 (PV00325) | 580/10 | 530/25 |
| Dichroic Mirror | 380 or 400 | 425 or 50/50 | 380 or 400 | 561 | 425 or 50/50 |
* Filters (diameter) or optics shown as center wavelength/bandwidth (nm) & dichroic mirrors shown as cutoff wavelength (nm); Contact Molecular Devices for information on ordering filters & dichroic mirrors suitable with the microplate readers listed above.
** Some compatible filter sets are available for purchase from Life Technologies. Click on selected filter sets for more information.
Analyst®, SpectraMax® and FlexStation® are registered trademarks of Molecular Devices.
For Research Use Only. Not for use in diagnostic procedures.