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NovaFluor Red 660 Dye
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| 4 | 640 | 660/20 | 637 | 658 | (in buffer) 5 | flow cytometry |
Invitrogen NovaFluor Red 660 dye has excitation and emission spectra similar to those of allophycocyanin (APC) and Alexa Fluor 647 dye. When excited by the red 640 nm laser line, the spectral spread of NovaFluor Red 660 dye is about half that of APC while maintaining similar brightness.
The macromolecule-based NovaFluor Red 660 dye produces highly stable fluorescence, and stained samples retain their fluorescence intensity and spectral signature when stored at 4°C. Use NovaFluor dyes with CellBlox Plus Blocking Buffer to reduce background and to block non-specific binding of NovaFluor labels, PE and APC tandems observed with macrophages and monocytes.
We offer NovaFluor dyes conjugated to primary antibodies for use in flow cytometry, as well as NovaFluor Antibody Conjugation Kits, NovaFluor CD4 Label Characterization Kits, and custom conjugation services.
- Narrow emission spectrum and minimal cross-laser excitation for better resolution
- Bright fluorescence allows it to be paired with medium-to-low abundance antigens
- Can serve as a replacement for APC with a cleaner emission spectrum, reducing spectral spillover for better marker resolution
- Can be used in a flow cytometry panel with NovaFluor Red 685 or NovaFluor Red 700 dye
- Produces highly stable fluorescence and is compatible with all buffers tested
Click image to enlarge
Spectral signature of NovaFluor Red 660 dye. Normal human peripheral blood cells stained with CD4 Monoclonal Antibody (SK3 (SK-3)), NovaFluor Red 660 were used for analysis. Data was acquired on a 5-laser Cytek Aurora system.
Staining of human peripheral blood cells with NovaFluor Red 660 conjugated antibody. Normal human peripheral blood cells (PBMCs) were unstained (left) or stained with CD127 Monoclonal Antibody, NovaFluor Red 660 (right). All cells were co-stained with CD3e Monoclonal Antibody, eFluor 450. Total viable cells in the lymphocyte gate were used for analysis, as determined by LIVE/DEAD Fixable Blue Dead Cell Stain Kit, for UV excitation. Data was acquired on a 5-laser Cytek Aurora and unmixed with autofluorescence extraction.
Additional resources
Flow cytometry protocols handbook
Protocols that fit your needs in flow cytometry ranging from sample preparation to numerous cell stimulation conditions, staining, immunophenotyping, and data analysis strategies.
Fluorophore and reagent selection guide for flow cytometry
A handy reference poster featuring the broad range of our available dyes and labeling reagents.
Imaging protocol handbook
Protocols that fit your needs in imaging ranging from sample and assay preparation to staining, labeling, and data analysis strategies.
Fluorophore and reagent selection guide for cell imaging
A tool for selecting the optimal fluorescent dyes for your Invitrogen EVOS cell imaging systems.
Cy™ is a trademark or registered trademark of GE Healthcare.
Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Brilliant Ultra Violet™ and Brilliant Violet™ are trademarks or registered trademarks of Becton, Dickinson and Company or its affiliates, and are used under license.
For Research Use Only. Not for use in diagnostic procedures.