基因體DNA萃取

為什麼推薦使用Thermo Fisher Scientific gDNA萃取試劑盒?

Thermo Fisher Scientific Invitrogen基因體DNA萃取試劑盒系列產品，提供高靈敏且可擴增的純化流程。我們的gDNA萃取試劑盒適用於多種樣品類型，可最大幅度提升製備效率與下游實驗結果。DNA萃取產品有多種類別的試劑盒，可從組織、細胞、血液、血清、植物、法醫鑑識樣本等各類型樣品中純化出基因體DNA。現在就為您的研究需求尋找適合的基因體DNA萃取試劑盒。

Three general methods for genomic DNA isolation are common, and each is based on a different biochemical principle. Selection of a method is based on the throughput required, equipment available in the lab, and how the purified genomic DNA will be used.
 

Genomic DNA isolation by organic extraction involves the addition of phenol and quanidine isothiocyanate to separate the DNA and proteins into different organic phases. Organic extraction is a low-cost method and, with advanced reagents such as DNAzol, is a straightforward method requiring very little equipment.
 

DNA binds to silica (aka glass fibers) under high-salt conditions and can be released under low-salt conditions. Silica-containing columns provide an easy way to bind, wash, and elute purified genomic DNA from multiple clarified cell lysates in parallel.

Columns are designed to flow buffers through centrifugation, vacuum, or gravity. PureLink Genomic DNA Mini Kits use spin column technology and work in a microcentrifuge for easy prep of up to 18 samples at a time. Spin plates provide the same isolation technology in a high-throughput, automation-friendly format.
 

In this method, paramagnetic (attracted to magnet) beads are added to the sample, and genomic DNA binds to the beads. Using a strong magnet, the beads are held in place while removing unwanted material. After washing, the genomic DNA is eluted from the beads in water or a low-salt buffer. The bead-based method, used in MagMAX multi-sample DNA isolation and related kits, is scalable and automation compatible.