Biotin Affinity Purification | Thermo Fisher Scientific

Biotin-Binding Affinity Resins, 87739 High Capacity Streptavidin Agarose

We offer a variety of resins for the purification of biotinylated or desthiobiotinylated proteins, peptides, and other molecules. These resins are available in multiple pack sizes, as well as in spin columns, kits, and coated plates for some ligands. Select products based on avidin, streptavidin, NeutrAvidin™, or CaptAvidin™ proteins.

Product choice—a variety of biotin binding choices to meet your purification needs
High performance—resins that maximize binding capacity and elution conditions
Flexible—available in multiple pack sizes

Choose the right biotin-binding resin for your experiment


	Avidin Agarose	Streptavidin Agarose	Streptavidin Agarose HC	NeutrAvidin Agarose	NeutrAvidin Agarose HC	Monomeric Avidin Agarose
Biotin binding sites	4	4	4	4	4	1
Binding capacity (biotinylated BSA)	≥20 µg/mL biotin	1–3 mg/mL	>10 mg/mL	1–2 mg/mL	>8 mg/mL	>1.2 mg/mL
Specificity	Low	Higher	Higher	Highest	Highest	High
Nonspecific binding	High	Lower	Lower	Lowest	Lowest	Low
Elution conditions	Harsh	Harsh	Harsh	Harsh	Harsh	Mild
	20219 (5 mL), 20225 (5 x 5 mL)	20347 (2 mL), 20349 (5 mL), 20353 (10 mL)	20357 (2 mL), 20359 (5 mL), 20361 (10 mL)	29200 (5 mL), 29201 (10 mL)	29202 (5 mL), 29204 (10 mL)	20228 (5 mL), 20267 (10 mL)

Need a coated microplate to capture biotinylated molecules for ELISA development and other plate-based assays?

We also offer a wide range of biotinylation reagents for proteins, peptides, antibodies, and other molecules.

Resources

For Research Use Only. Not for use in diagnostic procedures.