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Neurobiology Research Made Simple
Step 1: Isolate and grow
Media | Supplements | Substrates | Cell lines
Our broad neural cell offering includes mouse, rat, and human neural primary and stem cells, as well as accompanying Gibco media and supplements. All of our products have been developed to work together for optimal performance and to create complete neuronal, glial, and neural stem cell culture systems.
Gibco B-27 Plus Neuronal Culture System
The Gibco B-27 Plus Neuronal Culture System is a next-generation media system consisting of B27Plus Supplement and Neurobasal Plus increasing the survival of neurons by more than 50%.
The system offers:
- Increases survival of neurons in culture by more than 50%—the highest in the industry
- Accelerates neurite outgrowth and increases the number of synaptic-positive puncta
- Improves neuronal maturation and functionality
Superior Neuronal Cell Cultures
Gibco CultureOne Supplement is easily added to any conventional neuronal differentiation medium, such as the starter kit, to eliminate more than 75% of contaminating neural progenitor cells without any downstream impact.
CellCite Selection Tool
Match publications to products for your neuronal cells
The Gibco CellCite tool provides you with the most published cell culture and transfection products for your neuronal cell type. Find the products your peers used in their published research, along with the references, today.
Pluripotent Stem Cell Protocol Handbook
Begin with the fundamentals
At the onset of your research, it can be daunting to know where to begin. This handbook provides the protocols and solutions to get you started and guides you through reprogramming, culture, characterization, and more.
Gibco Neurobiology Protocol Handbook
Successful neurobiology experiments start here
Over 20 protocols from our experienced scientists are available in our updated Gibco Neurobiology Protocol Handbook.
Step 2: Modify
Transfection | Reprogramming
Transfecting primary neurons or reprogramming stem cells can be challenging due to low efficiencies. Low efficiencies result in additional work downstream. Improve your outcomes with the right reagents and protocols for your cells.
Common mRNA transfection myths debunked
Myth 1: mRNA is unstable and degrades quickly, requiring special handling
- A few simple precautions can help ensure mRNA stability: use RNase-free reagents and tips, aliquot and store mRNA at –80°C, and keep mRNA on ice when in use
Myth 2: mRNA is difficult to prepare
- The Invitrogen mMESSAGE mMACHINE T7 Ultra Transcription Kit provides 20–40 μg
of ready-to-use mRNA - Once prepared, the mRNA can be safely stored for later use in multiple transfection experiments
Myth 3: Transfection is much easier to perform with DNA than mRNA
- The protocol for Lipofectamine MessengerMAX reagent is straightforward, requiring the same
basic steps as protocols for DNA transfection reagents, and we offer a positive control GFP mRNA that can be used to evaluate the system - For difficult-to-transfect nondividing or primary cells, mRNA transfection obviates the need
for nuclear entry and improves protein expression and homogeneity
Myth 4: Exogenous mRNA elicits an immune response
- Chemically modified mRNAs, with 5-methylcytidine and pseudouridine modifications,
dramatically reduce innate immune response and improve mRNA translation
Transfection
Invitrogen Lipofectamine MessengerMAX Transfection Reagent
- Fast protein expression without the risk of genomic integration
- Optimized for transfection of neurons and primary cells
- Impressive performance: 60%+ transfection efficiency in neurons and primary cells
- Up to 10x higher cleavage efficiency using mRNA CRISPRs
Reprogramming and differentiation
Human neural cells can be accessed through the reprogramming of easily obtained fibroblasts into stem cells. These can then be differentiated into neural cells for further analysis. Increase your cell yield by at least 2-fold by combining high-efficiency reprogramming kits with optimized differentiation reagents.
Neurobiology Transfection Guide
The field of neurobiology is rapidly growing, with researchers providing new insights into a variety of areas essential for human health, including memory, mood disorders, aging, and disease. As the field moves toward more physiologically relevant models such as primary neurons and small animal models, the need for new nucleic acid delivery tools is rapidly increasing. Use our helpful Neurobiology Transfection Guide to assist you in this effort.
Step 3: Analyze
Imaging | Western blotting | ELISA
We offer a diverse collection of reagents and kits for neuroscience research, including fluorescent probes for dissecting neural anatomy and function, neuronal tracers, and a vast selection of neurobiology-related primary antibodies and kits.
Thermo Scientific CellInsight CX7 High-Content Analysis (HCA) Platform
Get the most from your precious samples
Acquire and analyze images simultaneously with this instrument’s widefield, brightfield, or confocal capabilities.
Invitrogen Countess II FL Automated Cell Counter
Ditch the clicker
Count cells, monitor fluorescent protein expression, and measure cell viability.
Additional products and resources
Antibody-based tools for neurodegenerative disease research handbook
Get access to detailed protocols for a wide range of experimental techniques, including western blotting, immunofluorescence, flow cytometry, ELISA, and more.
Western blotting systems
Complete solutions for the western blotting workflow.
Antibodies for all applications
Over 74,000 primary and secondary antibodies ready to pair up.
Invitrogen fluorescent reagents
Trusted products with a legacy bibliography.
Step 1: Isolate and grow
Media | Supplements | Substrates | Cell lines
Our broad neural cell offering includes mouse, rat, and human neural primary and stem cells, as well as accompanying Gibco media and supplements. All of our products have been developed to work together for optimal performance and to create complete neuronal, glial, and neural stem cell culture systems.
Gibco B-27 Plus Neuronal Culture System
The Gibco B-27 Plus Neuronal Culture System is a next-generation media system consisting of B27Plus Supplement and Neurobasal Plus increasing the survival of neurons by more than 50%.
The system offers:
- Increases survival of neurons in culture by more than 50%—the highest in the industry
- Accelerates neurite outgrowth and increases the number of synaptic-positive puncta
- Improves neuronal maturation and functionality
Superior Neuronal Cell Cultures
Gibco CultureOne Supplement is easily added to any conventional neuronal differentiation medium, such as the starter kit, to eliminate more than 75% of contaminating neural progenitor cells without any downstream impact.
CellCite Selection Tool
Match publications to products for your neuronal cells
The Gibco CellCite tool provides you with the most published cell culture and transfection products for your neuronal cell type. Find the products your peers used in their published research, along with the references, today.
Pluripotent Stem Cell Protocol Handbook
Begin with the fundamentals
At the onset of your research, it can be daunting to know where to begin. This handbook provides the protocols and solutions to get you started and guides you through reprogramming, culture, characterization, and more.
Gibco Neurobiology Protocol Handbook
Successful neurobiology experiments start here
Over 20 protocols from our experienced scientists are available in our updated Gibco Neurobiology Protocol Handbook.
Step 2: Modify
Transfection | Reprogramming
Transfecting primary neurons or reprogramming stem cells can be challenging due to low efficiencies. Low efficiencies result in additional work downstream. Improve your outcomes with the right reagents and protocols for your cells.
Common mRNA transfection myths debunked
Myth 1: mRNA is unstable and degrades quickly, requiring special handling
- A few simple precautions can help ensure mRNA stability: use RNase-free reagents and tips, aliquot and store mRNA at –80°C, and keep mRNA on ice when in use
Myth 2: mRNA is difficult to prepare
- The Invitrogen mMESSAGE mMACHINE T7 Ultra Transcription Kit provides 20–40 μg
of ready-to-use mRNA - Once prepared, the mRNA can be safely stored for later use in multiple transfection experiments
Myth 3: Transfection is much easier to perform with DNA than mRNA
- The protocol for Lipofectamine MessengerMAX reagent is straightforward, requiring the same
basic steps as protocols for DNA transfection reagents, and we offer a positive control GFP mRNA that can be used to evaluate the system - For difficult-to-transfect nondividing or primary cells, mRNA transfection obviates the need
for nuclear entry and improves protein expression and homogeneity
Myth 4: Exogenous mRNA elicits an immune response
- Chemically modified mRNAs, with 5-methylcytidine and pseudouridine modifications,
dramatically reduce innate immune response and improve mRNA translation
Transfection
Invitrogen Lipofectamine MessengerMAX Transfection Reagent
- Fast protein expression without the risk of genomic integration
- Optimized for transfection of neurons and primary cells
- Impressive performance: 60%+ transfection efficiency in neurons and primary cells
- Up to 10x higher cleavage efficiency using mRNA CRISPRs
Reprogramming and differentiation
Human neural cells can be accessed through the reprogramming of easily obtained fibroblasts into stem cells. These can then be differentiated into neural cells for further analysis. Increase your cell yield by at least 2-fold by combining high-efficiency reprogramming kits with optimized differentiation reagents.
Neurobiology Transfection Guide
The field of neurobiology is rapidly growing, with researchers providing new insights into a variety of areas essential for human health, including memory, mood disorders, aging, and disease. As the field moves toward more physiologically relevant models such as primary neurons and small animal models, the need for new nucleic acid delivery tools is rapidly increasing. Use our helpful Neurobiology Transfection Guide to assist you in this effort.
Step 3: Analyze
Imaging | Western blotting | ELISA
We offer a diverse collection of reagents and kits for neuroscience research, including fluorescent probes for dissecting neural anatomy and function, neuronal tracers, and a vast selection of neurobiology-related primary antibodies and kits.
Thermo Scientific CellInsight CX7 High-Content Analysis (HCA) Platform
Get the most from your precious samples
Acquire and analyze images simultaneously with this instrument’s widefield, brightfield, or confocal capabilities.
Invitrogen Countess II FL Automated Cell Counter
Ditch the clicker
Count cells, monitor fluorescent protein expression, and measure cell viability.
Additional products and resources
Antibody-based tools for neurodegenerative disease research handbook
Get access to detailed protocols for a wide range of experimental techniques, including western blotting, immunofluorescence, flow cytometry, ELISA, and more.
Western blotting systems
Complete solutions for the western blotting workflow.
Antibodies for all applications
Over 74,000 primary and secondary antibodies ready to pair up.
Invitrogen fluorescent reagents
Trusted products with a legacy bibliography.
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