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NucRed Live 647 ReadyProbes Reagent Protocol
Nuclear stain for live cells
NucRed Live 647 ReadyProbes Reagent is a bright far-red cell-permeant nuclear stain for live or fixed cells.
This protocol can be used for:
- Nuclear staining to identify nuclear condensation in apoptotic cells using fluorescence microscopy
This protocol should not be used for:
- Flow cytometry
You will need the following for this protocol:
- Cells growing in culture
- NucRed Live 647 ReadyProbes Reagent (Cat. No. R37106)
- Fluorescence microscope with far-red filter
Protocol
 1. Culture cells in an appropriate medium and vessel for fluorescence microscopy. |
 2. Add two drops of NucRed Live 647 Reagent per milliliter of medium. |
 3. Incubate for 15–30 minutes. |
 4. Image the cells. |
Spectral information and storage
| NucRed | |
|---|---|
| Excitation/Emission (nm) | 642/661 |
| Standard filter set | Cy®5 |
| EVOS Light Cube | Cy®5 |
| Storage conditions | Room temperature |
Protocol tips
- In some cases, more or fewer drops may be needed to achieve optimal staining intensity.
- Image quality may be improved by replacing the culture medium with Live Cell Imaging solution.
Nuclear staining in live hUVECs. hUVECs were grown overnight, stained with NucRed Live 647 ReadyProbes Reagent for 20 minutes at 37°C, and then imaged.