Phosphospecific Antibodies

Phosphorylation site-specific antibodies enable the analysis of key targets in normal and disease states, including cardiovascular disease, cancer, inflammation, neurodegenerative diseases, and diabetes. They are affinity-purified rabbit polyclonal and monoclonal antibodies that are monospecific for a target protein that is phosphorylated on a specific tyrosine, threonine, or serine residue. These tools are providing critical insight into the investigation of complex signal transduction events, including unraveling the intricacies of drug intervention in disease. Invitrogen phosphospecific antibodies are designed to dependably detect the key phosphospecific targets. Each antibody is validated for use in various applications.

• SRC
• FAK

• TAU
• EIF2

• AKT1
• ERK1/2

• JAK1
• STAT6

Immunofluorescent analysis of of Phospho-FAK (Tyr397). Visualizing focal adhesion kinase with Phospho-FAK (Tyr397) Polyclonal Antibody (Cat. No. 44-624G). HeLa cells were fixed and permeabilized using ice-cold 95% methanol. FAK (pY397) PSSA was labeled with Alexa Fluor 488 dye (green fluorescence, Zenon Rabbit IgG Labeling Kit, Cat. No. Z-25302), and nuclei were counterstained with DAPI (blue fluorescence, Cat. No. D21490). The image was captured using a Zeiss Axioplan 2 fluorescence microscope fitted with a 100× oil immersion lens.

ChIP-qPCR analysis of STAT1 (pTyr701) was performed with 5 µg of the Phospho-STAT1 (Tyr701) Monoclonal Antibody (ST1P-11A5) (Cat. No. 33-3400) on sheared chromatin from 2 million HeLa cells treated with 50 ng/mL of IFN Gamma for one hour using the MAGnify Chromatin Immunoprecipitation System (Cat. No. 49-2024). Normal Mouse IgG was used as a negative IP control. The purified DNA from each ChIP sample was analyzed by StepOnePlus Real-Time PCR System (Cat. No. 4376600) with primers for the promoter of active GLS1 gene, used as positive control target, and the inactive SAT2, used as negative control target. Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method.