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Alexa Fluor Secondary Antibodies |
Invitrogen Alexa Fluor protein and antibody conjugates produce fluorescence outputs that exceed those of other spectrally similar fluorophore conjugates and do not photobleach as easily as classic fluorescent secondary antibodies. With more than 80,000 publications, our Alexa Fluor secondary antibodies are reliable research tools. Browse our comprehensive portfolio of Alexa Fluor and Alexa Fluor Plus secondary antibodies for fluorescent detection of primary antibodies in a wide range of applications, such as cell imaging, flow cytometry, and western blotting.
Alexa Fluor secondary antibodies have been tested extensively to help ensure optimal performance. Alexa Fluor Plus secondary antibodies have been optimized further to enable better detection of low-abundance targets and are excellent for use in rare or precious samples.
Alexa Fluor Secondary Antibodies | Alexa Fluor Plus Secondary Antibodies | |
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Use | Designed for use in a variety of cell analysis and protein analysis applications to detect targets at varying expression levels |
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Key product attributes |
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Targets species available |
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Fluorophores available | 18 Alexa Fluor dyes available | 7 Alexa Fluor Plus dyes available |
Antibody formats available |
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Mountant compatibility | Most standard fixatives. See the selection guide: Mounting Media and Antifades Recommended: |
Our Alexa Fluor and Alexa Fluor Plus conjugated secondary antibodies are now available in a variety of formats, each offering unique benefits.
We offer F(ab) and F(ab’)2 fragment secondary antibodies with various Alexa Fluor conjugate options. These secondary antibody fragments are cross-adsorbed to increase sensitivity, reduce background staining, and offer several benefits over whole IgG antibodies. Because of their smaller size they can more easily penetrate complex tissue models compared to whole IgG molecules, potentially leading to more antigen binding capability. This property, combined with Alexa Fluor and Alexa Fluor Plus conjugation, makes these molecules valuable staining tools in immunohistochemistry and immunofluorescent experiments.
Superclonal recombinant secondary antibodies provide polyclonal sensitivity with monoclonal specificity in a recombinant format. Now available with a variety of Alexa Fluor and Alexa Fluor Plus conjugates, our Superclonal recombinant secondary antibodies help ensure reliable results with their lot-to-lot consistency.
ReadyProbes secondary antibodies are conjugated with bright and photostable Alexa Fluor 488 and Alexa Fluor 594 dyes to offer convenient and robust labeling. These Alexa Fluor secondary antibodies recognize IgG heavy chains and all classes of immunoglobulin light chains from either mouse or rabbit. Like other ReadyProbes reagents, these secondary antibodies are provided in a convenient dropper bottle format. No pipetting or dilutions are required.
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For Research Use Only. Not for use in diagnostic procedures.