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Superclonal Recombinant Secondary Antibodies |
What are Superclonal recombinant secondary antibodies?
Thermo Fisher Scientific Invitrogen Superclonal secondary antibodies represent a recombinant antibody technology designed to provide precise and accurate detection of mouse, rabbit and goat primary antibodies in a variety of applications.
Our proprietary screening and production process yields specific mixtures of recombinant goat or rabbit secondary antibodies that bind with the epitope-precision of monoclonal antibodies, while also achieving the multi-epitope coverage (e.g., H+L) and sensitivity of polyclonal antibodies. Superclonal secondary antibodies are in vitro manufactured using synthetic genes after the first immunization. Each Superclonal secondary antibody is formulated and optimized to help achieve excellent results in ELISA, cell and tissue imaging (ICC/IF and IHC) and flow cytometry applications.
Features of Superclonal recombinant secondary antibodies
- Developed from recombinant monoclonal antibodies to enable precise and accurate detection
- Formulated to recognize multiple epitopes of target protein
- Optimized for use with western blotting, ELISA, cell and tissue imaging, and flow cytometry applications
- Lot-to-lot consistency due to recombinant technology
- Available unconjugated and conjugated with biotin, horseradish peroxidase (HRP), alkaline phosphorate (AP), and selected Alexa Fluor and Alexa Fluor Plus dyes
Search Superclonal recombinant secondary antibodies by application
Product spotlight: Goat-anti-Human Superclonal recombinant secondary antibodies now available
Search our expanded menu of goat-anti-human Superclonal secondary antibodies, conjugated to Alexa Fluor and Alexa Fluor Plus dyes.
Compare Superclonal recombinant antibodies to conventional polyclonal antibodies
| Conventional polyclonal antibodies (pAb) | Superclonal recombinant antibodies | |
|---|---|---|
| How they are made | Affinity purification (by positive and/or negative selection) of antibodies from the serum of immunized animals. | Developed in vitro by considered characterization and selection methods. Superclonal secondary antibodies contain a pool of numerous individual recombinant antibody clones. |
| What they are | A large, undefined pool of antibodies from the host serum, selected by affinity purification. Contain multiple epitopes, the exact numbers of which are generally undetermined. | Precisely characterized sets of specific recombinant antibodies having complementary sets of epitopes and affinity binding features. |
| Epitope coverage and signal amplification | Broad epitope coverage for the target antibody helps ensure good sensitivity and signal. | Pooled recombinant antibodies profiled to provide specific and sensitive binding and improved product quality that helps maximize performance across applications. |
| Specificity | Based on the individual host animal used for immunization, specificity of pAb can differ vastly. Affinity purification of pAb from the source serum provides some additional improvement of specificity. | Individual clones and the final pool of clones are screened (using positive and/or negative selection) to eliminate cross-reactivity and help ensure high specificity in key applications. |
| Lot-to-lot consistency | Animal variability and purification process can result in variability between lots. | Recombinant antibody technology enables high lot-to-lot consistency. |
Image gallery of Superclonal recombinant secondary antibody data
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Superclonal secondary antibodies are designed to provide lot-to-lot consistency. Endogenous TOMM20 in HeLa cells were labeled with rabbit TOMM20 monoclonal primary antibody, which was then detected with four different lots of Goat anti-Rabbit IgG (Heavy Chain), Superclonal Recombinant Secondary Antibody, Alexa Fluor Plus 488 (Cat. No. A55053) (green). Comparable signal and noise fluorescence intensities from these four lots demonstrate lot-to-lot consistency of Superclonal secondary antibodies. Inset represents control cells with no primary (NP) antibody to assess noise.
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Superclonal secondary antibodies are designed to provide enhanced specificity. Endogenous HDAC2 in HeLa cells were labeled with mouse HDAC2 monoclonal primary antibody, which was then detected with Goat anti-Mouse IgG (H+L), Superclonal Recombinant Secondary Antibody, Alexa Fluor Plus 647 (Cat. No. A55060) (red). Simultaneously, LRP130 was labeled with rabbit LRP130 antibody followed by anti-rabbit secondary antibody conjugated to Alexa Fluor Plus 488 (green). Goat anti-Mouse IgG (H+L), Superclonal Recombinant Secondary Antibody, Alexa Fluor Plus 647 (Cat. No. A55060) specifically detects mouse primary antibody and does not cross-react with rabbit primary antibody.
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Immunohistochemical analysis of Goat-anti-Human IgG (H+L), Superclonal Recombinant Secondary Antibody, Alexa Fluor 488 (Cat. No. A66724). Analysis was performed on rat testis FFPE sections. De-paraffinized sections were subjected to heat induced epitope retrieval using eBioscience IHC Antigen Retrieval Solution - Low pH (10X) (Cat. No. 00-4955-58) and blocked with 2% normal goat serum for 1 hour at room temperature. Sections were then probed for HSPA-4 overnight at 4oC. This was detected by incubating with Goat-anti-Human IgG (H+L), Superclonal Recombinant Secondary, Antibody Alexa Fluor 488 (Cat. No. A66724, used at 2 µg/mL) in 0.1% normal goat serum for 1 hour at room temperature. The images were captured on EVOS M7000 Imaging System (Cat. No. AMF7000) at 20X magnification. Panel a) represents the composite image of HSPA-4 (green) and the nuclear stain (blue). Panel b) represents the composite image of no primary antibody control and the nuclear stain (blue).
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Immunohistochemical analysis of Goat anti-Human IgG (H+L), Superclonal Recombinant Secondary Antibody, Alexa Fluor 647 (Cat. No. A66725). Analysis was performed on rat testis FFPE sections. De-paraffinized sections were subjected to heat induced epitope retrieval using eBioscience IHC Antigen Retrieval Solution - Low pH (10X) (Cat. No. 00-4955-58) and blocked with 2% normal goat serum for 1 hour at room temperature. Sections were then probed for HSPA-4 overnight at 4oC. This was detected by incubating with Goat anti-Human IgG (H+L), Superclonal Recombinant Secondary Antibody, Alexa Fluor 647 (Cat. No. A66725, used at 2 µg/mL) in 0.1% normal goat serum for 45 minutes at room temperature. The images were captured on EVOS M7000 Imaging System (Cat. No. AMF7000) at 20X magnification. Panel a) represents the composite image of HSPA-4 (red) and the nuclear stain (blue). Panel b) represents the composite image of no primary antibody control and the nuclear stain (blue).
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Immunohistochemical analysis of Goat anti-Human IgG Fc, Recombinant Secondary Antibody, Alexa Fluor Plus 488 (Cat. No. A66726). Analysis was performed on rat testis FFPE sections. De-paraffinized sections were subjected to heat induced epitope retrieval using eBioscience IHC Antigen Retrieval Solution - Low pH (10X) (Cat. No. 00-4955-58) and blocked with 2% normal goat serum for 1 hour at room temperature. Sections were then probed for HSPA-4 overnight at 4oC. This was detected by incubating with Goat anti-Human IgG Fc, Recombinant Secondary Antibody, Alexa Fluor Plus 488 (Cat. No. A66726, used at 2 µg/mL) in 0.1% normal goat serum for 45 minutes at room temperature. The images were captured on EVOS M7000 Imaging System (Cat. No. AMF7000) at 20X magnification. Panel a) shows the specific detection of HSPA-4 (green) in the nucleus and cytoplasm of Leydig cells and seminiferous duct cells in testis. Panel b) shows absence of non-specific staining in the presence of secondary antibody alone. Panel c) represents the composite image of HSPA-4 (green) and the nuclear stain (blue). Panel d) represents the composite image of no primary antibody control and the nuclear stain (blue).
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Immunofluorescence analysis comparing the Alexa Fluor 488 and Alexa Fluor Plus 488 conjugates of Goat-anti-Human IgG (H+L), Superclonal Recombinant Secondary Antibody. Analysis was performed on fixed and permeabilized T-47D cells blocked with 2% BSA at room temperature and stained for MUC-1 protein using Gatipotuzumab Recombinant Human Monoclonal Antibody (Cat. No. MA5-42157, used at 1:500 dilution) in 0.1% BSA overnight at 4-degree Celsius. This was detected by incubating with Goat-anti-Human IgG (H+L), Superclonal Recombinant Secondary Antibody, Alexa Fluor 488 (Cat. No. A66724) or Goat anti-Human IgG (H+L), Superclonal Recombinant Secondary Antibody, Alexa Fluor Plus 488 (Cat. No. A56021) at 1:1,000 dilution. Panel a) represents the composite images of cells that were stained for detection and localization of MUC-1 protein using Goat-anti-Human IgG (H+L), Superclonal Recombinant Secondary Antibody, Alexa Fluor 488 (green) and the nuclear counterstain (red). Panel b) represents the composite images of cells that were stained for detection and localization of MUC-1 protein using Goat anti-Human IgG (H+L), Superclonal Recombinant Secondary Antibody, Alexa Fluor Plus 488 (green) and the nuclear counterstain (red). The images were captured at 20X magnification on CellInsight CX7 LZR High Content Analysis Platform (Cat. No. CX7A1110LZR).
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