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EVOS FLoid Imaging System
Catalog number: 4471136
Designed to be one of the simplest, fastest, and easiest cell imaging systems in the world, the EVOS FLoid captures high-quality, 3-color fluorescent cell images right at your benchtop within a minute. Usage is so intuitive, even novice users can collect data in just a few mouse clicks. The EVOS FLoid is ideal for cell culture applications requiring fluorescence, or for just giving you a quick view of your cells.
Features
The EVOS FLoid system shares common features with other EVOS microscopes while simplifying the choice of options. Instead of a choice of LED light cubes, it comes with fixed blue, green, and red LED light sources and filters compatible with the most common reagents and fluorochromes used in fluorescence microscopy. Instead of a choice of objectives, it comes with a fixed 20x objective that supports 460x optical magnification and up to 1,840x digital zoom.
Easy self-installation
Self-installation in as little as 10 minutes with no maintenance, assembly, alignment, or calibration
Basic color options
3-color fluorescence with focus assist and adjustable focus, brightness, and contrast
Multilingual
Your choice of seven interface languages
Speed
Get images within a minute, without warmup, cool-down, or filter changes
Benchtop
Instrument design blocks ambient light, taking fluorescent cell imaging out of the darkroom and onto your benchtop
Self-contained
No moving parts, long-life LEDs for long-term reliability through day-to-day use
Integrated reagent selection guide
With protocols
Attached printer
Print high-quality color images and paste them right in your lab notebook
Enhanced safety
Many operational functions can be controlled through software, reducing the need to touch the instrument
Videos & demos
View more EVOS systems videos and demos under Resources.
Featured Images
Reagent compatibility
Designed to be one of the simplest, fastest, and easiest cell imaging systems in the world, the EVOS FLoid system captures high-quality, 3-color fluorescent cell images right at your benchtop within a minute. Usage is so intuitive, even novice users can collect data in just a few mouse clicks. The EVOS FLoid system is ideal for cell culture applications requiring fluorescence, or for just giving you a quick view of your cells.
EVOS FLoid system light sources and common compatible dyes
| Light source | Excitation (nm) | Emission (nm) | Compatible dyes |
| Blue | 390/40 | 446/33 | DAPI, Hoechst stain, NucBlue, Alexa Fluor 350, BFP |
| Green | 482/18 | 532/59 | GFP, Alexa Fluor 488, Calcein, SYBR Green, FITC, Fluo-4, pHrodo Green |
| Red | 586/15 | 646/68 | RFP, Alexa Fluor 546/555/568, PE, Cy3, MitoTracker Orange, pHrodo Red, Rhodamine Red, DsRed |
For a more complete list of compatible reagents and applications, see EVOS FLoid reagent compatibility under Resources.
Specifications
| Category | Attribute | Description |
| Optics | Objective | 20X plan fluorite; |
| Numerical aperture (N/A) | 0.45 | |
| Working distance (WD) | 5.9 mm (defined as the tip of the objective to the bottom of a 1.2-mm thick slide) | |
| Overall magnification | 460X (optical)-1840X (with digital zoom) | |
| Display resolution | 1366 x 768 pixels | |
| Image resolution | 1296 x 964 pixels |
|
| Contrast methods | Fluorescence and transmitted light (relief phase) | |
| Color channels | 4 channels (relief phase, blue fluorescence, green fluorescence, red fluorescence) | |
| Illumination | LED (50,000 hour life), adjustable intensity | |
| Excitation | Blue channel: 390/40 nm; Green channel: 482/18 nm; Red channel: 586/15 nm | |
| Emission | Blue channel: 446/33 nm; Green channel: 532/59 nm; Red channel: 646/68 nm | |
| Stage to condenser | 60 nm | |
| Camera | Sony 1.3MP 1/3” ICX445 EXview HAD CCD | |
| Physical characteristics | Instrument type | Benchtop device for imaging cells |
Instrument dimensions |
15.9” (W) x 21.1” (H) x 13.9” (D) 40.4 cm (W) x 53.6 cm (H) x 35.3 cm (D) |
|
| Weight | 26 lbs (11.8 kg) | |
| Operating power | 100-240 VAC |
|
| Frequency | 50-60 Hz | |
| Electrical input | 5 VDC, 4.15 A | |
| Operating temperature | 4-32ºC | |
| Operating humidity | <90% (non-condensing) | |
| Output ports | 4 USB | |
| Hardware/Software | Stage | Mechanical XY glide stage that can move within a 5 mm diameter circle |
| LCD Monitor | 15-inch color; display resolution 1366 x 768 pixels; Image resolution 1296 x 964 pixels; adjustable tilt | |
| Software | FLoid Cell Imaging Station Software |
|
| Captured images | 16-bit monochrome TIFF, PNG, BMP and JPG; 1296 x 964 pixels | |
| Supplied USB drive | 2 Gigabyte |
For Research Use Only. Not for use in diagnostic procedures.