Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
Neuronal culture supplements are mixtures of nutrients and vitamins that, when added to a basal medium, create a complete neural cell culture system. Gibco neuronal culture supplements provide high quality and consistency to support your neural cell culture and experiments.
N-2 supplement was originally designed and demonstrated to support neuroblastoma (nerve cell cancer) cell lines in serum-free culture whereas B-27 supplement was developed specifically for culture of primary embryonic neurons.
The N-2 supplement formulation includes insulin, transferrin, progesterone, putrescine, and selenium. The B-27 supplement formulation includes many of the same components as N-2 supplement, but also includes the thyroid hormone T3, fatty acids, and antioxidants, such as vitamin E and glutathione.
Thermo Fisher Scientific recommends using the specified Gibco cell culture supplements for the following applications:
Cell type | Specific cell type/application | Supplement |
---|---|---|
Astrocytes and glia | Culture of primary astrocytes | G-5 Supplement |
Culture of glial cell lines of astrocytic phenotype | G-5 Supplement | |
Neurons | Growth of pre-natal/fetal primary neurons | B-27 Plus Supplement |
Maintenance/maturation of post-natal and adult brain neurons | B-27 Plus Supplement | |
Selection of neurons from mixed neural progenitor populations; control of glial cell outgrowth | CultureOne Supplement | |
Maintenance/maturation of stem cell-derived neurons | B-27 Plus Supplement and CultureOne Supplement | |
Differentiation and maturation of stem cell-derived neurons | B-27 Supplement | |
Studies of oxidative stress/damage, apoptosis, or where free radical damage to neurons occurs | B-27 Supplement without AO (antioxidants) | |
Electrophysiology studies | B-27 Plus Supplement | |
Neural cells | Growth and expansion of retinal ganglion cells | B-27 Supplement, Custom |
Studies of insulin secretion or insulin receptors | B-27 Supplement without insulin | |
Proliferation of neural stem cells | B-27 Supplement without vitamin A | |
Studies where xenobiotic-free media components are required | B-27 Supplement, XenoFree | |
Neuroblastoma and other tumor cells of neuronal phenotype | N-2 Supplement |
For Research Use Only. Not for use in diagnostic procedures.