Invitrogen

CD3 Monoclonal Antibody (UCHT1), PE-Cyanine5, eBioscience™

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This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

34 Published Figures

34 References

View all (504) CD3 antibodies

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CD3 Antibody (15-0038-42) in Flow

Staining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control PE-Cyanine5 (Product # 15-4714-81) (open histogram) or Anti-Human CD3 PE-Cyanine5 (filled histogram).Cells in the lymphocyte gate were used for analysis.

Product Image Gallery

CD3 Antibody — Staining of human peripheral blood cells. As expected based on known relative expression patterns, CD3 clone UCHT1 stains a subset of lymphocytes (T cells) and does not stain monocytes and granulocytes. Details: Normal human whole blood was surface stained with CD3 (clone UCHT1). After staining, red blood cells were lysed using 1-step Fix/Lyse Buffer. Cells in the lymphocyte (purple histogram), monocyte (orange histogram), or granulocyte (blue histogram) gates were used for analysis. {RE}

Product Details

15-0038-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.06 µg)/test

View 34 publications 34 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Rat

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE-Cyanine5, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

UCHT1

Conjugate

PE-Cyanine5 PE-Cyanine5 PE-Cyanine5

Excitation/Emission Max

568/666 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10598354

Product Specific Information

Description: The UCHT1 monoclonal antibody reacts with human CD3e, a 20 kDa subunit of the TCR complex. Along with the other CD3 subunits gamma and delta, the epsilon chain is required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Crosslinking of TCR via immobilized UCHT1 initiates an intracellular biochemical pathway resulting in cellular activation and proliferation.

Applications Reported: The UCHT1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This UCHT1 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 667 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The CD3 subunit complex which is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules and this association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. The genes encoding the CD3 epsilon, gamma and delta polypeptides are located on chromosome 11. Defects in the CD3 gene are associated with CD3 immunodeficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Aberrant gut-microbiota-immune-brain axis development in premature neonates with brain damage.

Cell host & microbe

Seki D,Mayer M,Hausmann B,Pjevac P,Giordano V,Goeral K,Unterasinger L,Klebermaß-Schrehof K,De Paepe K,Van de Wiele T,Spittler A,Kasprian G,Warth B,Berger A,Berry D,Wisgrill L

Published figure using CD3 monoclonal antibody (Product # 15-0038-42) in Flow Cytometry

Wed Oct 13 00:00:00 EDT 2021

Single-cell RNA sequencing reveals ex vivo signatures of SARS-CoV-2-reactive T cells through 'reverse phenotyping'.

Nature communications

Fischer DS,Ansari M,Wagner KI,Jarosch S,Huang Y,Mayr CH,Strunz M,Lang NJ,D'Ippolito E,Hammel M,Mateyka L,Weber S,Wolff LS,Witter K,Fernandez IE,Leuschner G,Milger K,Frankenberger M,Nowak L,Heinig-Menhard K,Koch I,Stoleriu MG,Hilgendorff A,Behr J,Pichlmair A,Schubert B,Theis FJ,Busch DH,Schiller HB,Schober K

Published figure using CD3 monoclonal antibody (Product # 15-0038-42) in Flow Cytometry

Mon Jul 26 00:00:00 EDT 2021

Single-cell transcriptomics reveals regulators underlying immune cell diversity and immune subtypes associated with prognosis in nasopharyngeal carcinoma.

Cell research

Chen YP,Yin JH,Li WF,Li HJ,Chen DP,Zhang CJ,Lv JW,Wang YQ,Li XM,Li JY,Zhang PP,Li YQ,He QM,Yang XJ,Lei Y,Tang LL,Zhou GQ,Mao YP,Wei C,Xiong KX,Zhang HB,Zhu SD,Hou Y,Sun Y,Dean M,Amit I,Wu K,Kuang DM,Li GB,Liu N,Ma J

Published figure using CD3 monoclonal antibody (Product # 15-0038-42) in Flow Cytometry

Sun Nov 01 00:00:00 EDT 2020

Aging affects responsiveness of peripheral blood mononuclear cells to immunosuppression of periodontal ligament stem cells.

The Journal of international medical research

Li X,Zhang B,Wang H,Zhao X,Zhang Z,Ding G,Wei F

Published figure using CD3 monoclonal antibody (Product # 15-0038-42) in Flow Cytometry

Wed Jul 01 00:00:00 EDT 2020

Involvement of natural killer cells in the pathogenesis of endometriosis in patients with pelvic pain.

The Journal of international medical research

He J,Xu Y,Yi M,Gu C,Zhu Y,Hu G

Published figure using CD3 monoclonal antibody (Product # 15-0038-42) in Flow Cytometry

Wed Jul 01 00:00:00 EDT 2020

Dendritic Cell Maturation Regulates TSPAN7 Function in HIV-1 Transfer to CD4⁺ T Lymphocytes.

Frontiers in cellular and infection microbiology

Perot BP,García-Paredes V,Luka M,Ménager MM

Published figure using CD3 monoclonal antibody (Product # 15-0038-42) in Flow Cytometry

Wed Mar 17 00:00:00 EDT 2021

Th2-like T Follicular Helper Cells Promote Functional Antibody Production during Plasmodium falciparum Infection.

Cell reports. Medicine

Chan JA,Loughland JR,de Labastida Rivera F,SheelaNair A,Andrew DW,Dooley NL,Wines BD,Amante FH,Webb L,Hogarth PM,McCarthy JS,Beeson JG,Engwerda CR,Boyle MJ

Published figure using CD3 monoclonal antibody (Product # 15-0038-42) in Flow Cytometry

Tue Dec 22 00:00:00 EST 2020

Development of CAR-T cell therapy for B-ALL using a point-of-care approach.

Oncoimmunology

de Macedo Abdo L,Barros LRC,Saldanha Viegas M,Vieira Codeço Marques L,de Sousa Ferreira P,Chicaybam L,Bonamino MH

15-0038 was used in Flow cytometry/Cell sorting to demonstrate that the POC approach is a viable alternative for the generation and use of CAR-T cells, overcoming the limitations of current manufacturing protocols.

Wed Jul 14 00:00:00 EDT 2021

Loss of ARPC1B impairs cytotoxic T lymphocyte maintenance and cytolytic activity.

The Journal of clinical investigation

Randzavola LO,Strege K,Juzans M,Asano Y,Stinchcombe JC,Gawden-Bone CM,Seaman MN,Kuijpers TW,Griffiths GM

Published figure using CD3 monoclonal antibody (Product # 15-0038-42) in Flow Cytometry

Mon Dec 02 00:00:00 EST 2019

Skewed T cell responses to Epstein-Barr virus in long-term asymptomatic kidney transplant recipients.

PloS one

Nakid-Cordero C,Arzouk N,Gauthier N,Tarantino N,Larsen M,Choquet S,Burrel S,Autran B,Vieillard V,Guihot A

Published figure using CD3 monoclonal antibody (Product # 15-0038-42) in Flow Cytometry

Mon Mar 30 00:00:00 EDT 2020

Inflammation induced by incomplete radiofrequency ablation accelerates tumor progression and hinders PD-1 immunotherapy.

Nature communications

Shi L,Wang J,Ding N,Zhang Y,Zhu Y,Dong S,Wang X,Peng C,Zhou C,Zhou L,Li X,Shi H,Wu W,Long X,Wu C,Liao W

Published figure using CD3 monoclonal antibody (Product # 15-0038-42) in Flow Cytometry

Thu Nov 28 00:00:00 EST 2019

Ultra-High-Frequency Reprogramming of Individual Long-Term Hematopoietic Stem Cells Yields Low Somatic Variant Induced Pluripotent Stem Cells.

Cell reports

Wang K,Guzman AK,Yan Z,Zhang S,Hu MY,Hamaneh MB,Yu YK,Tolu S,Zhang J,Kanavy HE,Ye K,Bartholdy B,Bouhassira EE

15-0038-42 was used in Flow Cytometry to suggest LT-HSCs may become the preferred cell source for the production of clinical-grade iPSCs.

Tue Mar 05 00:00:00 EST 2019

Biallelic mutations in DNA ligase 1 underlie a spectrum of immune deficiencies.

The Journal of clinical investigation

Maffucci P,Chavez J,Jurkiw TJ,O'Brien PJ,Abbott JK,Reynolds PR,Worth A,Notarangelo LD,Felgentreff K,Cortes P,Boisson B,Radigan L,Cobat A,Dinakar C,Ehlayel M,Ben-Omran T,Gelfand EW,Casanova JL,Cunningham-Rundles C

Published figure using CD3 monoclonal antibody (Product # 15-0038-42) in Flow Cytometry

Mon Dec 03 00:00:00 EST 2018

Abnormal expression of circulating and tumor-infiltrating carcinoembryonic antigen-related cell adhesion molecule 1 in patients with glioma.

Oncology letters

Li J,Liu X,Duan Y,Wang H,Su W,Wang Y,Zhuang G,Fan Y

15-0038-42 was used in Flow cytometry/Cell sorting to analyse associations between CEACAM1 expression and multiple clinicopathological characteristics in patients with gliomas.

Thu Mar 01 00:00:00 EST 2018

Th17 and IL-17 exhibit higher levels in osteonecrosis of the femoral head and have a positive correlation with severity of pain.

Endokrynologia Polska

Zou D,Zhang K,Yang Y,Ren Y,Zhang L,Xiao X,Zhang H,Liu S,Li J

15-0038-42 was used in Flow cytometry/Cell sorting to analyze the proinflammatory mediation expression of IL-17 and Th17, which perform key functions in regulating inflammatory processes in the inflamed synovium and peripheral blood in osteonecrosis of the femoral head.

Wed Oct 31 00:00:00 EDT 2018

Blocking the recruitment of naive CD4⁺ T cells reverses immunosuppression in breast cancer.

Cell research

Su S,Liao J,Liu J,Huang D,He C,Chen F,Yang L,Wu W,Chen J,Lin L,Zeng Y,Ouyang N,Cui X,Yao H,Su F,Huang JD,Lieberman J,Liu Q,Song E

Published figure using CD3 monoclonal antibody (Product # 15-0038-42) in Flow Cytometry

Sat Apr 01 00:00:00 EDT 2017

Ricolinostat, the First Selective Histone Deacetylase 6 Inhibitor, in Combination with Bortezomib and Dexamethasone for Relapsed or Refractory Multiple Myeloma.

Clinical cancer research : an official journal of the American Association for Cancer Research

Vogl DT,Raje N,Jagannath S,Richardson P,Hari P,Orlowski R,Supko JG,Tamang D,Yang M,Jones SS,Wheeler C,Markelewicz RJ,Lonial S

15-0038 was used in Flow cytometry to define the safety, preliminary efficacy, and recommended phase II dose of selective histone deacetylase 6 inhibition in combination with standard proteasome inhibitor therapy.

Sat Jul 01 00:00:00 EDT 2017

Follicular Regulatory T Cells Are Highly Permissive to R5-Tropic HIV-1.

Journal of virology

Miller SM,Miles B,Guo K,Folkvord J,Meditz AL,McCarter MD,Levy DN,MaWhinney S,Santiago ML,Connick E

Published figure using CD3 monoclonal antibody (Product # 15-0038-42) in Flow Cytometry

Fri Sep 01 00:00:00 EDT 2017

Antigen receptor-redirected T cells derived from hematopoietic precursor cells lack expression of the endogenous TCR/CD3 receptor and exhibit specific antitumor capacities.

Oncoimmunology

Van Caeneghem Y,De Munter S,Tieppo P,Goetgeluk G,Weening K,Verstichel G,Bonte S,Taghon T,Leclercq G,Kerre T,Debets R,Vermijlen D,Abken H,Vandekerckhove B

Published figure using CD3 monoclonal antibody (Product # 15-0038-42) in Flow Cytometry

Tue Jan 12 00:00:00 EST 2021

Interaction among activated lymphocytes and mesenchymal cells through podoplanin is critical for a high IL-17 secretion.

Arthritis research & therapy

Noack M,Ndongo-Thiam N,Miossec P

Published figure using CD3 monoclonal antibody (Product # 15-0038-42) in Flow Cytometry

Thu Jun 23 00:00:00 EDT 2016

Increased circulating IL-9-producing CD8⁺ T cells are associated with eosinophilia and high FeNO in allergic asthmatics.

Experimental and therapeutic medicine

Wang W,Cheng ZS,Chen YF,Lin YH

15-0038 was used in Flow cytometry/Cell sorting to investigate the involvement of IL-9-producing CD8+ cytotoxic T (Tc9) cells in allergic asthma immunopathogenesis.

Thu Dec 01 00:00:00 EST 2016

MAIT cells are activated during human viral infections.

Nature communications

van Wilgenburg B,Scherwitzl I,Hutchinson EC,Leng T,Kurioka A,Kulicke C,de Lara C,Cole S,Vasanawathana S,Limpitikul W,Malasit P,Young D,Denney L,Moore MD,Fabris P,Giordani MT,Oo YH,Laidlaw SM,Dustin LB,Ho LP,Thompson FM,Ramamurthy N,Mongkolsapaya J,Willberg CB,Screaton GR,Klenerman P

Published figure using CD3 monoclonal antibody (Product # 15-0038-42) in Flow Cytometry

Thu Jun 23 00:00:00 EDT 2016

Follicular regulatory T cells impair follicular T helper cells in HIV and SIV infection.

Nature communications

Miles B,Miller SM,Folkvord JM,Kimball A,Chamanian M,Meditz AL,Arends T,McCarter MD,Levy DN,Rakasz EG,Skinner PJ,Connick E

Published figure using CD3 monoclonal antibody (Product # 15-0038-42) in Flow Cytometry

Tue Oct 20 00:00:00 EDT 2015

The disturbance of TH17-Treg cell balance in adenomyosis.

Fertility and sterility

Gui T,Chen C,Zhang Z,Tang W,Qian R,Ma X,Cao P,Wan G

15-0038 was used in Flow cytometry/Cell sorting to investigate the balance between Treg and T(H)17 cells in peripheral blood and uteri of women with adenomyosis.

Sat Feb 01 00:00:00 EST 2014

Human mesenchymal stromal cells modulate T-cell responses through TNF-α-mediated activation of NF-κB.

European journal of immunology

Dorronsoro A,Ferrin I,Salcedo JM,Jakobsson E,Fernández-Rueda J,Lang V,Sepulveda P,Fechter K,Pennington D,Trigueros C

Published figure using CD3 monoclonal antibody (Product # 15-0038-42) in Flow Cytometry

Sat Feb 01 00:00:00 EST 2014

A comparison of DNA methylation specific droplet digital PCR (ddPCR) and real time qPCR with flow cytometry in characterizing human T cells in peripheral blood.

Epigenetics

Wiencke JK,Bracci PM,Hsuang G,Zheng S,Hansen H,Wrensch MR,Rice T,Eliot M,Kelsey KT

Published figure using CD3 monoclonal antibody (Product # 15-0038-42) in Flow Cytometry

Wed Oct 01 00:00:00 EDT 2014

Trypan blue exclusion assay by flow cytometry.

Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas

Avelar-Freitas BA,Almeida VG,Pinto MC,Mourão FA,Massensini AR,Martins-Filho OA,Rocha-Vieira E,Brito-Melo GE

Published figure using CD3 monoclonal antibody (Product # 15-0038-42) in Flow Cytometry

Tue Apr 01 00:00:00 EDT 2014

Induction of type I and type III interferons by Borrelia burgdorferi correlates with pathogenesis and requires linear plasmid 36.

PloS one

Krupna-Gaylord MA,Liveris D,Love AC,Wormser GP,Schwartz I,Petzke MM

Published figure using CD3 monoclonal antibody (Product # 15-0038-42) in Flow Cytometry

Thu Mar 12 00:00:00 EDT 2015

Novel lentiviral vectors with mutated reverse transcriptase for mRNA delivery of TALE nucleases.

Scientific reports

Mock U,Riecken K,Berdien B,Qasim W,Chan E,Cathomen T,Fehse B

Published figure using CD3 monoclonal antibody (Product # 15-0038-42) in Flow Cytometry

Thu Sep 18 00:00:00 EDT 2014

Recombinant influenza virus carrying the respiratory syncytial virus (RSV) F85-93 CTL epitope reduces RSV replication in mice.

Journal of virology

De Baets S,Schepens B,Sedeyn K,Schotsaert M,Roose K,Bogaert P,Fiers W,Saelens X

Published figure using CD3 monoclonal antibody (Product # 15-0038-42) in Flow Cytometry

Fri Mar 01 00:00:00 EST 2013

MHC multimer-guided and cell culture-independent isolation of functional T cell receptors from single cells facilitates TCR identification for immunotherapy.

PloS one

Dössinger G,Bunse M,Bet J,Albrecht J,Paszkiewicz PJ,Weißbrich B,Schiedewitz I,Henkel L,Schiemann M,Neuenhahn M,Uckert W,Busch DH

Published figure using CD3 monoclonal antibody (Product # 15-0038-42) in Flow Cytometry

Mon Nov 11 00:00:00 EST 2013

Epigenetic biomarkers of T-cells in human glioma.

Epigenetics

Wiencke JK,Accomando WP,Zheng S,Patoka J,Dou X,Phillips JJ,Hsuang G,Christensen BC,Houseman EA,Koestler DC,Bracci P,Wiemels JL,Wrensch M,Nelson HH,Kelsey KT

Published figure using CD3 monoclonal antibody (Product # 15-0038-42) in Flow Cytometry

Sat Dec 01 00:00:00 EST 2012

Human tonsil B-cell lymphoma 6 (BCL6)-expressing CD4+ T-cell subset specialized for B-cell help outside germinal centers.

Proceedings of the National Academy of Sciences of the United States of America

Bentebibel SE,Schmitt N,Banchereau J,Ueno H

15-0038 was used in Flow cytometry/Cell sorting to identify and investigate the role of a previously undefined BCL6-expressing CD4(+) T-cell subset in human tonsils.

Tue Aug 16 00:00:00 EDT 2011

Frequency of gC1qR+CD4+ T cells increases during acute hepatitis C virus infection and remains elevated in patients with chronic infection.

Clinical immunology (Orlando, Fla.)

Cummings KL,Rosen HR,Hahn YS

15-0038 was used in Flow cytometry/Cell sorting to suggest that HCV infection expands gC1qR+CD4+ T cells, which ultimately facilitates the establishment of HCV persistency.

Tue Sep 01 00:00:00 EDT 2009

Bioinformatics

Protein Aliases: CD247; CD247 antigen, zeta subunit; CD3 antigen, delta subunit; CD3 delta; CD3 TCR complex; CD3-epsilon; CD3d; CD3d antigen, delta polypeptide (TiT3 complex); CD3d molecule, delta (CD3-TCR complex); CD3e; CD3e antigen, epsilon polypeptide (TiT3 complex); CD3e molecule, epsilon (CD3-TCR complex); CD3g; CD3g antigen, gamma polypeptide (TiT3 complex); CD3g molecule, epsilon (CD3-TCR complex); CD3g molecule, gamma (CD3-TCR complex); CD3Z antigen, zeta polypeptide (TiT3 complex); CD3zeta chain; FLJ18683; Leu-4; OKT3, delta chain; T cell antigen receptor complex epsilon subunit of T3; T-cell antigen receptor complex, epsilon subunit of T3; T-cell antigen receptor complex, gamma subunit of T3; T-cell antigen receptor complex, zeta subunit of CD3; T-cell receptor T3 delta chain; T-cell receptor T3 gamma chain; T-cell receptor T3 zeta chain; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 delta chain; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface glycoprotein CD3 gamma chain; T-cell surface glycoprotein CD3 zeta chain; T3/TCR complex; TCR zeta chain

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Gene Aliases: CD247; CD3-DELTA; CD3-GAMMA; CD3-ZETA; CD3D; CD3E; CD3G; CD3H; CD3Q; CD3Z; IMD17; IMD18; IMD19; IMD25; T3D; T3E; T3G; T3Z; TCRE; TCRZ

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UniProt ID: (Human) P20963, (Human) P04234, (Human) P07766, (Human) P09693

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Entrez Gene ID: (Human) 919, (Human) 915, (Human) 916, (Human) 917

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Function(s)

transmembrane signaling receptor activity protein binding identical protein binding protein homodimerization activity transcription coactivator activity protein heterodimerization activity receptor signaling protein activity SH3 domain binding protein kinase binding receptor signaling complex scaffold activity T cell receptor binding defense/immunity protein immunoglobulin receptor superfamily

Process(es)

Performance Guarantee

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CD3 Monoclonal Antibody (UCHT1), PE-Cyanine5, eBioscience™

CD3 Antibody (15-0038-42) in Flow

Product Details

15-0038-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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