Invitrogen

CD3 Monoclonal Antibody (UCHT1), eFluor™ 450, eBioscience™

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This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

30 References

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CD3 Antibody (48-0038-42) in Flow

Staining of human peripheral blood mononuclear cells with CD45 PE, CD19 APC and CD3 eFluor 450. As expected based on known relative expression patterns, CD3 clone UCHT1 stains a subset of lymphocytes (pink), but not monocytes (orange) and granulocytes (blue).

Product Image Gallery

CD3 Antibody — Staining of human peripheral blood cells. As expected based on known relative expression patterns, CD3 clone UCHT1 stains a subset of lymphocytes (T cells) and does not stain monocytes and granulocytes. Details: Normal human whole blood was surface stained with CD3 (clone UCHT1). After staining, red blood cells were lysed using 1-step Fix/Lyse Buffer. Cells in the lymphocyte (purple histogram), monocyte (orange histogram), or granulocyte (blue histogram) gates were used for analysis. {RE}

Product Details

48-0038-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.5 µg)/test

View 30 publications 30 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), eFluor™ 450, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

UCHT1

Conjugate

eFluor™ 450 eFluor™ 450 eFluor™ 450

Excitation/Emission Max

405/445 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1518798

Product Specific Information

Description: The UCHT1 monoclonal antibody reacts with human CD3e, a 20 kDa subunit of the TCR complex. Along with the other CD3 subunits gamma and delta, the epsilon chain is required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Crosslinking of TCR via immobilized UCHT1 initiates an intracellular biochemical pathway resulting in cellular activation and proliferation.

Applications Reported: This UCHT1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This UCHT1 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

eFluor® 450 is a replacement for Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The CD3 subunit complex which is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules and this association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. The genes encoding the CD3 epsilon, gamma and delta polypeptides are located on chromosome 11. Defects in the CD3 gene are associated with CD3 immunodeficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Enhancement of PD-L1-attenuated CAR-T cell function through breast cancer-associated fibroblasts-derived IL-6 signaling via STAT3/AKT pathways.

Breast cancer research : BCR

Chuangchot N,Jamjuntra P,Yangngam S,Luangwattananun P,Thongchot S,Junking M,Thuwajit P,Yenchitsomanus PT,Thuwajit C

48-0038-42 was used in Flow cytometry/Cell sorting to highlight CAF-derived IL-6 in the resistance of chemotherapy and T cell therapy.

Fri Jul 21 00:00:00 EDT 2023

Optimization of the proliferation and persistency of CAR T cells derived from human induced pluripotent stem cells.

Nature biomedical engineering

Ueda T,Shiina S,Iriguchi S,Terakura S,Kawai Y,Kabai R,Sakamoto S,Watanabe A,Ohara K,Wang B,Xu H,Minagawa A,Hotta A,Woltjen K,Uemura Y,Kodama Y,Seno H,Nakatsura T,Tamada K,Kaneko S

48-0038-42 was used in Flow cytometry/Cell sorting to show that the proliferation and persistency of the effector cells in the tumours can be further enhanced by genetically knocking out diacylglycerol kinase, which inhibits antigen-receptor signalling, and by transducing the cells with genes encoding for membrane-bound interleukin-15 (IL-15) and its receptor subunit IL-15Rα.

Sun Jan 01 00:00:00 EST 2023

SARS-CoV-2 infection paralyzes cytotoxic and metabolic functions of the immune cells.

Heliyon

Singh Y,Trautwein C,Fendel R,Krickeberg N,Berezhnoy G,Bissinger R,Ossowski S,Salker MS,Casadei N,Riess O

48-0038-42 was used in Flow Cytometry to study the effects of SARS-CoV-2 virus infection on immune and metabolic functions in human cells.

Tue Jun 01 00:00:00 EDT 2021

Single-Cell Analyses Inform Mechanisms of Myeloid-Targeted Therapies in Colon Cancer.

Cell

Zhang L,Li Z,Skrzypczynska KM,Fang Q,Zhang W,O'Brien SA,He Y,Wang L,Zhang Q,Kim A,Gao R,Orf J,Wang T,Sawant D,Kang J,Bhatt D,Lu D,Li CM,Rapaport AS,Perez K,Ye Y,Wang S,Hu X,Ren X,Ouyang W,Shen Z,Egen JG,Zhang Z,Yu X

48-0038-42 was used in Flow Cytometry to identify critical cellular interactions regulating tumor immunity and defines mechanisms underlying myeloid-targeted immunotherapies currently undergoing clinical testing.

Thu Apr 16 00:00:00 EDT 2020

Single-Cell Analyses Inform Mechanisms of Myeloid-Targeted Therapies in Colon Cancer.

Cell

Thu Apr 16 00:00:00 EDT 2020

Deficient IL-2 Produced by Activated CD56⁺ T Cells Contributes to Impaired NK Cell-Mediated ADCC Function in Chronic HIV-1 Infection.

Frontiers in immunology

Xie Z,Zheng J,Wang Y,Li D,Maermaer T,Li Y,Tu J,Xu Q,Liang H,Cai W,Shen T

48-0038 was used in Flow cytometry/Cell sorting to identify that the reduced NK cell-mediated ADCC function in HIV-1 infection is due to the reduced ability of activated CD56+ T cells to secrete IL-2.

Wed Oct 14 00:00:00 EDT 2020

Two phase kinetics of the inflammatory response from hepatocyte-peripheral blood mononuclear cell interactions.

Scientific reports

Beringer A,Molle J,Bartosch B,Miossec P

48-0038 was used in Flow cytometry/Cell sorting to study the role of interactions between non and activated human peripheral blood mononuclear cells and human hepatoma HepaRG cells in the inflammatory response.

Mon Jun 10 00:00:00 EDT 2019

The Changing Landscape of Naive T Cell Receptor Repertoire With Human Aging.

Frontiers in immunology

Egorov ES,Kasatskaya SA,Zubov VN,Izraelson M,Nakonechnaya TO,Staroverov DB,Angius A,Cucca F,Mamedov IZ,Rosati E,Franke A,Shugay M,Pogorelyy MV,Chudakov DM,Britanova OV

48-0038 was used in Flow cytometry/Cell sorting to study the differences in the architecture of TCR beta repertoires with human aging.

Wed Nov 20 00:00:00 EST 2019

Human Immunodeficiency Virus Infection Impairs Th1 and Th17 Mycobacterium tuberculosis-Specific T-Cell Responses.

The Journal of infectious diseases

Murray LW,Satti I,Meyerowitz J,Jones M,Willberg CB,Ussher JE,Goedhals D,Hurst J,Phillips RE,McShane H,Vuuren CV,Frater J

48-0038 was used in Flow cytometry/Cell sorting to measure expression of interferon gamma, tumor necrosis factor alpha, interleukin 2, and interleukin 17 in CD4+ and CD8+ T cells in response to Mtb antigens (PPD, ESAT-6/CFP-10 [EC], and DosR regulon-encoded α-crystallin [Rv2031c]).

Sat May 05 00:00:00 EDT 2018

Human cancer evolution in the context of a human immune system in mice.

Molecular oncology

Gammelgaard OL,Terp MG,Preiss B,Ditzel HJ

48-0038 was used in Flow cytometry/Cell sorting to suggest that HIS mice may provide a promising in vivo tumor model for evaluating immune modulatory anticancer therapies.

Mon Oct 01 00:00:00 EDT 2018

CXCL4 is a novel inducer of human Th17 cells and correlates with IL-17 and IL-22 in psoriatic arthritis.

European journal of immunology

Affandi AJ,Silva-Cardoso SC,Garcia S,Leijten EFA,van Kempen TS,Marut W,van Roon JAG,Radstake TRDJ

48-0038 was used in Flow cytometry/Cell sorting to study whether CXCL4 plays a direct role in the induction of IL-17 production by human CD4+ T cells.

Thu Mar 01 00:00:00 EST 2018

The NRF2 pathway as potential biomarker for dimethyl fumarate treatment in multiple sclerosis.

Annals of clinical and translational neurology

Hammer A,Waschbisch A,Kuhbandner K,Bayas A,Lee DH,Duscha A,Haghikia A,Gold R,Linker RA

48-0038 was used in Flow cytometry/Cell sorting to demonstrate that positive effects of DMF on the clinical outcome are paralleled by induction of the antioxidant NRF2 transcriptional pathway and a shift toward regulatory immune cell subsets in the periphery.

Fri Jun 01 00:00:00 EDT 2018

Establishment of a novel platform cell line for efficient and precise evaluation of T cell receptor functional avidity.

Oncotarget

Morimoto S,Fujiki F,Kondo K,Nakajima H,Kobayashi Y,Inatome M,Aoyama N,Nishida Y,Tsuboi A,Oka Y,Nishida S,Nakata J,Hosen N,Oji Y,Sugiyama H

48-0038 was used in Flow cytometry/Cell sorting to develop a novel platform cell line, named 2D3, which enables the functional avidity of transduced T cell receptors to be evaluated efficiently and precisely.

Tue Sep 25 00:00:00 EDT 2018

Immunosuppressive Mesenchymal Stromal Cells Derived from Human-Induced Pluripotent Stem Cells Induce Human Regulatory T Cells In Vitro and In Vivo.

Frontiers in immunology

Roux C,Saviane G,Pini J,Belaïd N,Dhib G,Voha C,Ibáñez L,Boutin A,Mazure NM,Wakkach A,Blin-Wakkach C,Rouleau M

48-0038 was used in Flow cytometry/Cell sorting to examine the immunosuppressive activity of human induced pluripotent stem cell derived-mesenchymal stromal cells on human T-cell stimulation with a concomitant generation of human regulatory T cells in vivo.

Wed Nov 20 00:00:00 EST 2019

Eomesodermin and T-bet mark developmentally distinct human natural killer cells.

JCI insight

Collins A,Rothman N,Liu K,Reiner SL

48-0038 was used in Flow cytometry/Cell sorting to demonstrate the expression pattern of signature NK cell transcription factors during developmental maturation.

Thu Mar 09 00:00:00 EST 2017

Vitamin D deficiency is associated with IL-6 levels and monocyte activation in HIV-infected persons.

PloS one

Manion M,Hullsiek KH,Wilson EMP,Rhame F,Kojic E,Gibson D,Hammer J,Patel P,Brooks JT,Baker JV,Sereti I

48-0038 was used in Flow cytometry/Cell sorting to study the relationship between persistent inflammation and immune activation during chronic HIV infection and vitamin D deficiency.

Tue Sep 05 00:00:00 EDT 2017

Role of podoplanin in the high interleukin-17A secretion resulting from interactions between activated lymphocytes and psoriatic skin-derived mesenchymal cells.

Clinical and experimental immunology

Noack M,Ndongo-Thiam N,Miossec P

48-0038-42 was used in Flow cytometry/Cell sorting to study the interactions of skin-derived fibroblasts with peripheral blood mononuclear cells (PBMC) with a focus on the Th17 pathway and to identify a mechanism which leads to a high interleukin (IL)-17 secretion.

Sat Oct 01 00:00:00 EDT 2016

Failure recovery of circulating NKG2D⁺CD56^dimNK cells in HBV-associated hepatocellular carcinoma after hepatectomy predicts early recurrence.

Oncoimmunology

Gao J,Duan Z,Zhang L,Huang X,Long L,Tu J,Liang H,Zhang Y,Shen T,Lu F

48-0038 was used in Flow cytometry/Cell sorting to analyse changes in natural killer cell profile in peripheral blood after surgery and in the tumour tissues of hepatocellular carcinoma patients.

Tue Jan 12 00:00:00 EST 2021

Antitumor immunity triggered by melphalan is potentiated by melanoma cell surface-associated calreticulin.

Cancer research

Dudek-Perić AM,Ferreira GB,Muchowicz A,Wouters J,Prada N,Martin S,Kiviluoto S,Winiarska M,Boon L,Mathieu C,van den Oord J,Stas M,Gougeon ML,Golab J,Garg AD,Agostinis P

48-0038 was used in Flow cytometry/Cell sorting to illustrate how melphalan triggers inflammatory cell death that can be leveraged by immunomodulators.

Wed Apr 15 00:00:00 EDT 2015

CD8+ T Cells Can Mediate Short-Term Protection against Heterotypic Dengue Virus Reinfection in Mice.

Journal of virology

Zellweger RM,Tang WW,Eddy WE,King K,Sanchez MC,Shresta S

48-0038 was used in Flow cytometry/Cell sorting to investigate short-term protection against heterotypic Dengue virus reinfection in mice mediated by CD8+ T Cells.

Mon Jun 01 00:00:00 EDT 2015

Vitamin D deficiency contributes to the reduction and impaired function of naïve CD45RA⁺ regulatory T cell in chronic heart failure.

Journal of immunology research

Ma YH,Zhou YL,Yue CY,Zhang GH,Deng L,Xie GH,Xu WP,Shen LS

48-0038 was used in Flow cytometry/Cell sorting to investigate the impact of vitamin D supplementation on naïve Tregs.

Tue Jan 26 00:00:00 EST 2016

Children with asthma by school age display aberrant immune responses to pathogenic airway bacteria as infants.

The Journal of allergy and clinical immunology

Larsen JM,Brix S,Thysen AH,Birch S,Rasmussen MA,Bisgaard H

48-0038 was used in Flow cytometry/Cell sorting to assess the immune responses to pathogen bacteria in children suffering from asthma.

Tue Apr 01 00:00:00 EDT 2014

Monocyte-activation phenotypes are associated with biomarkers of inflammation and coagulation in chronic HIV infection.

The Journal of infectious diseases

Wilson EM,Singh A,Hullsiek KH,Gibson D,Henry WK,Lichtenstein K,Önen NF,Kojic E,Patel P,Brooks JT,Sereti I,Baker JV

48-0038 was used in Flow cytometry/Cell sorting to indicate the association of monocyte-activation phenotypes with biomarkers of inflammation and coagulation in chronic HIV infection.

Sat Nov 01 00:00:00 EDT 2014

Evidence for innate immune system activation in HIV type 1-infected elite controllers.

The Journal of infectious diseases

Krishnan S,Wilson EM,Sheikh V,Rupert A,Mendoza D,Yang J,Lempicki R,Migueles SA,Sereti I

48-0038 was used in Flow cytometry/Cell sorting to study how elite controllers maintain control of plasma HIV viremia and have evidence of an activated innate immune response.

Sat Mar 01 00:00:00 EST 2014

Decreases in colonic and systemic inflammation in chronic HIV infection after IL-7 administration.

PLoS pathogens

Sereti I,Estes JD,Thompson WL,Morcock DR,Fischl MA,Croughs T,Beq S,Lafaye de Micheaux S,Yao MD,Ober A,Wilson EM,Natarajan V,Imamichi H,Boulassel MR,Lederman MM,Routy JP

48-0038 was used in Flow cytometry/Cell sorting to test the efficacy of IL-7 adjunctive therapy on T-cell reconstitution in antiretroviral therapy-suppressed HIV patients.

Wed Jan 01 00:00:00 EST 2014

A truncation variant of the cation channel P2RX5 is upregulated during T cell activation.

PloS one

Abramowski P,Ogrodowczyk C,Martin R,Pongs O

48-0038 was used in Flow cytometry/Cell sorting to show that P2RX5 gene expression of human T lymphocytes is upregulated during activation.

Mon May 18 00:00:00 EDT 2015

ROS-induced autophagy in cancer cells assists in evasion from determinants of immunogenic cell death.

Autophagy

Garg AD,Dudek AM,Ferreira GB,Verfaillie T,Vandenabeele P,Krysko DV,Mathieu C,Agostinis P

48-0038 was used in Flow cytometry/Cell sorting to unravel a role for ROS-induced autophagy in weakening functional interaction between dying cancer cells and the immune system.

Sun Sep 01 00:00:00 EDT 2013

Comprehensive analysis of dengue virus-specific responses supports an HLA-linked protective role for CD8+ T cells.

Proceedings of the National Academy of Sciences of the United States of America

Weiskopf D,Angelo MA,de Azeredo EL,Sidney J,Greenbaum JA,Fernando AN,Broadwater A,Kolla RV,De Silva AD,de Silva AM,Mattia KA,Doranz BJ,Grey HM,Shresta S,Peters B,Sette A

48-0038 was used in Flow cytometry/Cell sorting to analyse dengue virus-specific responses, supporting an HLA-linked protective role for CD8+ T cells.

Tue May 28 00:00:00 EDT 2013

Memory T cells in latent Mycobacterium tuberculosis infection are directed against three antigenic islands and largely contained in a CXCR3+CCR6+ Th1 subset.

PLoS pathogens

Lindestam Arlehamn CS,Gerasimova A,Mele F,Henderson R,Swann J,Greenbaum JA,Kim Y,Sidney J,James EA,Taplitz R,McKinney DM,Kwok WW,Grey H,Sallusto F,Peters B,Sette A

48-0038 was used in Flow cytometry/Cell sorting to further define the immunological footprint of Mycobacterium tuberculosis CD4 T cell recognition.

Tue Jan 01 00:00:00 EST 2013

Ribavirin Does Not Impair the Suppressive Activity of Foxp3(+)CD4(+)CD25(+) Regulatory T Cells.

Immune network

Lee J,Choi YS,Shin EC

48-0038 was used in Flow cytometry/Cell sorting to demonstrate that ribavirin did not attenuate the suppressive activity of Foxp3(+)CD4(+)CD25(+) Treg cells.

Fri Feb 01 00:00:00 EST 2013

Characterization of Th17 responses to Streptococcus pneumoniae in humans: comparisons between adults and children in a developed and a developing country.

Vaccine

Lundgren A,Bhuiyan TR,Novak D,Kaim J,Reske A,Lu YJ,Qadri F,Malley R

48-0038 was used in Flow cytometry/Cell sorting to compare different Th17 responses to pneumococcal antigens and whole cell antigens, in children and adults in both developed and developing countries.

Wed Jun 06 00:00:00 EDT 2012

Bioinformatics

Protein Aliases: CD247; CD247 antigen, zeta subunit; CD3 antigen, delta subunit; CD3 delta; CD3 TCR complex; CD3-epsilon; CD3d; CD3d antigen, delta polypeptide (TiT3 complex); CD3d molecule, delta (CD3-TCR complex); CD3e; CD3e antigen, epsilon polypeptide (TiT3 complex); CD3e molecule, epsilon (CD3-TCR complex); CD3g; CD3g antigen, gamma polypeptide (TiT3 complex); CD3g molecule, epsilon (CD3-TCR complex); CD3g molecule, gamma (CD3-TCR complex); CD3Z antigen, zeta polypeptide (TiT3 complex); CD3zeta chain; FLJ18683; Leu-4; OKT3, delta chain; T cell antigen receptor complex epsilon subunit of T3; T-cell antigen receptor complex, epsilon subunit of T3; T-cell antigen receptor complex, gamma subunit of T3; T-cell antigen receptor complex, zeta subunit of CD3; T-cell receptor T3 delta chain; T-cell receptor T3 gamma chain; T-cell receptor T3 zeta chain; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 delta chain; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface glycoprotein CD3 gamma chain; T-cell surface glycoprotein CD3 zeta chain; T3/TCR complex; TCR zeta chain

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Gene Aliases: CD247; CD3-DELTA; CD3-GAMMA; CD3-ZETA; CD3D; CD3E; CD3G; CD3H; CD3Q; CD3Z; IMD17; IMD18; IMD19; IMD25; T3D; T3E; T3G; T3Z; TCRE; TCRZ

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UniProt ID: (Human) P20963, (Human) P04234, (Human) P07766, (Human) P09693

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Entrez Gene ID: (Human) 919, (Human) 915, (Human) 916, (Human) 917

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Function(s)

transmembrane signaling receptor activity protein binding identical protein binding protein homodimerization activity transcription coactivator activity protein heterodimerization activity receptor signaling protein activity SH3 domain binding protein kinase binding receptor signaling complex scaffold activity T cell receptor binding defense/immunity protein immunoglobulin receptor superfamily

Process(es)

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CD3 Monoclonal Antibody (UCHT1), eFluor™ 450, eBioscience™

CD3 Antibody (48-0038-42) in Flow

Product Details

48-0038-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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