Invitrogen

CD27 Monoclonal Antibody (LG.7F9), FITC, eBioscience™

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Antibody Testing Data (1)
Published Figures (18)

FIGURE: 1 / 19

CD27 Antibody (11-0271-82) in Flow

Staining of C57Bl/6 splenocytes with Anti-Human/Mouse CD45R (B220) eFluor® 450 (Product # 48-0452-82) and 0.25 µg of Armenian Hamster IgG Isotype Control FITC (Product # 11-4888-81) (left) or 0.25 µg of Anti-CD27 FITC (right). Total viable cells were used for analysis.

Product Image Gallery

Product Details

11-0271-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.5 µg/test

View 31 publications 31 publications

Functional Assay (Functional)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Armenian hamster / IgG

Recommended Isotype Control

Armenian Hamster IgG Isotype Control (eBio299Arm), FITC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

LG.7F9

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_465001

Product Specific Information

Description: The LG.7F9 monoclonal antibody reacts with mouse CD27, a lymphocyte-specific member of the TNFR superfamily. CD27 is expressed by virtually all mature T cells and by a subpopulation of B cells, mainly memory B cells. In mouse, CD27 has been found on nearly all thymocytes excluding a population of CD46-CD8- precursors. CD27 binds to CD70 and, through this interaction, plays an important role in T cell-B cell interaction. It has been reported that triggering CD27 plays an important role in the maturation of CD4+ and CD8+ effector cells. LG.7F9 cross-reacts with human and rat CD27.

Applications Reported: The LG.7F9 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The LG.7F9 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD27 is a 50 kDa member of the tumor necrosis factor (TNF) receptor superfamily that includes CD40 and CD30. The TNF superfamily members are known for the regulation of cell proliferation and death. In contrast to the expression of other TNFR/TNF family members, expression of CD27 and its ligand CD70 is predominantly confined to lymphocytes. High expression levels of CD27 appear to be dependent on proper ligation of antigen receptors. CD70 expression requires additional co-stimulatory and/or pro-inflammatory signals. CD27 is expressed as a disulfide-linked homodimer on mature thymocytes, peripheral blood T cells and a subpopulation of B cells. Activation of T cells via TCR-CD3 complex results in upregulation of CD27 expression on the plasma membrane as well as in the release of its soluble 28-32 kDa form, sCD27, detected in the plasma, urine or spinal fluid. Soluble CD27 is an important prognostic marker of acute and chronic B cell malignancies. RgpA, a cystein proteinase, although activating T cells through the protease-activated receptors (PARs), degradates CD27 and counteracts T cell activation mediated by CD27 and its ligand CD70. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor. Diseases associated with CD27 dysfunction include Lymphoproliferative Syndrome 2 and Autosomal Recessive Lymphoproliferative Syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

GSK-3 Inhibitor Elraglusib Enhances Tumor-Infiltrating Immune Cell Activation in Tumor Biopsies and Synergizes with Anti-PD-L1 in a Murine Model of Colorectal Cancer.

International journal of molecular sciences

Huntington KE,Louie AD,Srinivasan PR,Schorl C,Lu S,Silverberg D,Newhouse D,Wu Z,Zhou L,Borden BA,Giles FJ,Dooner M,Carneiro BA,El-Deiry WS

11-0271-82 was used in Flow cytometry/Cell sorting to investigate the immunomodulatory properties of the GSK-3 inhibitor elraglusib, revealing its capacity to enhance immune cell-mediated tumor cell killing, sensitize colorectal cancer cells to immune-mediated cytotoxicity, reduce immunosuppressive factors, and improve survival when combined with immune checkpoint blockade therapy in preclinical models and clinical samples.

Thu Jun 29 00:00:00 EDT 2023

The transcription factor Bach2 negatively regulates murine natural killer cell maturation and function.

eLife

Li S,Bern MD,Miao B,Fan C,Xing X,Inoue T,Piersma SJ,Wang T,Colonna M,Kurosaki T,Yokoyama WM

Published figure using CD27 monoclonal antibody (Product # 11-0271-82) in Flow Cytometry

Mon Oct 03 00:00:00 EDT 2022

The transcriptional repressor ID2 supports natural killer cell maturation by controlling TCF1 amplitude.

The Journal of experimental medicine

Li ZY,Morman RE,Hegermiller E,Sun M,Bartom ET,Maienschein-Cline M,Sigvardsson M,Kee BL

Published figure using CD27 monoclonal antibody (Product # 11-0271-82) in Flow Cytometry

Mon Jun 07 00:00:00 EDT 2021

Chronic T cell proliferation in brains after stroke could interfere with the efficacy of immunotherapies.

The Journal of experimental medicine

Heindl S,Ricci A,Carofiglio O,Zhou Q,Arzberger T,Lenart N,Franzmeier N,Hortobagyi T,Nelson PT,Stowe AM,Denes A,Edbauer D,Liesz A

Published figure using CD27 monoclonal antibody (Product # 11-0271-82) in Flow Cytometry

Mon Aug 02 00:00:00 EDT 2021

PI3Kδ coordinates transcriptional, chromatin, and metabolic changes to promote effector CD8⁺ T cells at the expense of central memory.

Cell reports

Cannons JL,Villarino AV,Kapnick SM,Preite S,Shih HY,Gomez-Rodriguez J,Kaul Z,Shibata H,Reilley JM,Huang B,Handon R,McBain IT,Gossa S,Wu T,Su HC,McGavern DB,O'Shea JJ,McGuire PJ,Uzel G,Schwartzberg PL

Published figure using CD27 monoclonal antibody (Product # 11-0271-82) in Flow Cytometry

Tue Oct 12 00:00:00 EDT 2021

Effect of Subcutaneous Anti-CD20 Antibody-Mediated B Cell Depletion on Susceptibility to Pneumocystis Infection in Mice.

mSphere

Dai G,Noell K,Weckbecker G,Kolls JK

11-0271 was used in Flow cytometry/Cell sorting to show that low-dose subcutaneous anti-CD20 can modulate B cell populations without grossly perturbing fungal immunity against Pneumocystis lung infection.

Wed May 05 00:00:00 EDT 2021

Effect of Subcutaneous Anti-CD20 Antibody-Mediated B Cell Depletion on Susceptibility to Pneumocystis Infection in Mice.

mSphere

Dai G,Noell K,Weckbecker G,Kolls JK

Wed May 05 00:00:00 EDT 2021

Immune cell phenotypes associated with disease severity and long-term neutralizing antibody titers after natural dengue virus infection.

Cell reports. Medicine

Rouers A,Chng MHY,Lee B,Rajapakse MP,Kaur K,Toh YX,Sathiakumar D,Loy T,Thein TL,Lim VWX,Singhal A,Yeo TW,Leo YS,Vora KA,Casimiro D,Lim B,Tucker-Kellogg L,Rivino L,Newell EW,Fink K

Published figure using CD27 monoclonal antibody (Product # 11-0271-82) in Flow Cytometry

Tue May 18 00:00:00 EDT 2021

Development of a VRC01-class germline targeting immunogen derived from anti-idiotypic antibodies.

Cell reports

Seydoux E,Wan YH,Feng J,Wall A,Aljedani S,Homad LJ,MacCamy AJ,Weidle C,Gray MD,Brumage L,Taylor JJ,Pancera M,Stamatatos L,McGuire AT

Published figure using CD27 monoclonal antibody (Product # 11-0271-82) in Flow Cytometry

Tue May 04 00:00:00 EDT 2021

Ontogenic timing, T cell receptor signal strength, and Notch signaling direct γδ T cell functional differentiation in vivo.

Cell reports

Chen ELY,Lee CR,Thompson PK,Wiest DL,Anderson MK,Zúñiga-Pflücker JC

Published figure using CD27 monoclonal antibody (Product # 11-0271-82) in Flow Cytometry

Tue Jun 08 00:00:00 EDT 2021

Combination blockade of KLRG1 and PD-1 promotes immune control of local and disseminated cancers.

Oncoimmunology

Tata A,Dodard G,Fugère C,Leget C,Ors M,Rossi B,Vivier E,Brossay L

11-0271-82 was used in Flow Cytometry to demonstrate if killer cell lectin-like receptor G1 is a novel checkpoint inhibitor target that affects NK and T cell anti-tumor immunity.

Tue Jun 15 00:00:00 EDT 2021

Th2 Biased Immunity With Altered B Cell Profiles in Circulation of Patients With Sporotrichosis Caused by Sporothrix globosa.

Frontiers in immunology

Zu J,Yao L,Song Y,Cui Y,Guan M,Chen R,Zhen Y,Li S

Published figure using CD27 monoclonal antibody (Product # 11-0271-82) in Flow Cytometry

Mon May 31 00:00:00 EDT 2021

Inflammation-Induced Lactate Leads to Rapid Loss of Hepatic Tissue-Resident NK Cells.

Cell reports

Dodard G,Tata A,Erick TK,Jaime D,Miah SMS,Quatrini L,Escalière B,Ugolini S,Vivier E,Brossay L

11-0271 was used in Flow cytometry/Cell sorting to characterize the kinetics and immune response of each of the hepatic NK cell populations to viral challenge and other stimuli.

Tue Jul 07 00:00:00 EDT 2020

Commensal Microbiota Promote Lung Cancer Development via γδ T Cells.

Cell

Jin C,Lagoudas GK,Zhao C,Bullman S,Bhutkar A,Hu B,Ameh S,Sandel D,Liang XS,Mazzilli S,Whary MT,Meyerson M,Germain R,Blainey PC,Fox JG,Jacks T

11-0271 was used in Flow cytometry/Cell sorting to link local microbiota-immune crosstalk to lung tumor development and thereby define key cellular and molecular mediators that may serve as effective targets in lung cancer intervention.

Thu Feb 21 00:00:00 EST 2019

DKK2 imparts tumor immunity evasion through β-catenin-independent suppression of cytotoxic immune-cell activation.

Nature medicine

Xiao Q,Wu J,Wang WJ,Chen S,Zheng Y,Yu X,Meeth K,Sahraei M,Bothwell ALM,Chen L,Bosenberg M,Chen J,Sexl V,Sun L,Li L,Tang W,Wu D

11-0271 was used in Flow Cytometry to identify a novel tumour immune-suppressive mechanism and immunotherapeutic targets particularly relevant for colorectal cancers and a subset of melanomas.

Thu Mar 01 00:00:00 EST 2018

BALDR: a computational pipeline for paired heavy and light chain immunoglobulin reconstruction in single-cell RNA-seq data.

Genome medicine

Upadhyay AA,Kauffman RC,Wolabaugh AN,Cho A,Patel NB,Reiss SM,Havenar-Daughton C,Dawoud RA,Tharp GK,Sanz I,Pulendran B,Crotty S,Lee FE,Wrammert J,Bosinger SE

Published figure using CD27 monoclonal antibody (Product # 11-0271-82) in Flow Cytometry

Tue Mar 20 00:00:00 EDT 2018

Crosstalks between mTORC1 and mTORC2 variagate cytokine signaling to control NK maturation and effector function.

Nature communications

Wang F,Meng M,Mo B,Yang Y,Ji Y,Huang P,Lai W,Pan X,You T,Luo H,Guan X,Deng Y,Yuan S,Chu J,Namaka M,Hughes T,Ye L,Yu J,Li X,Deng Y

Published figure using CD27 monoclonal antibody (Product # 11-0271-82) in Flow Cytometry

Mon Nov 19 00:00:00 EST 2018

Phenotypic characterization of regulatory T cells from antiretroviral-naive HIV-1-infected people.

Immunology

Ambada GN,Ntsama CE,Nji NN,Ngu LN,Sake CN,Lissom A,Tchouangeu FT,Tchadji J,Sosso M,Etoa FX,Nchinda GW

11-0271 was used in Flow cytometry/Cell sorting to determine how antiretroviral therapy-naive HIV-1 infection affects the phenotypic properties of Treg cells.

Tue Aug 01 00:00:00 EDT 2017

The breast tumor microenvironment alters the phenotype and function of natural killer cells.

Cellular & molecular immunology

Krneta T,Gillgrass A,Chew M,Ashkar AA

Published figure using CD27 monoclonal antibody (Product # 11-0271-82) in Flow Cytometry

Thu Sep 01 00:00:00 EDT 2016

NK cell development requires Tsc1-dependent negative regulation of IL-15-triggered mTORC1 activation.

Nature communications

Yang M,Chen S,Du J,He J,Wang Y,Li Z,Liu G,Peng W,Zeng X,Li D,Xu P,Guo W,Chang Z,Wang S,Tian Z,Dong Z

Published figure using CD27 monoclonal antibody (Product # 11-0271-82) in Flow Cytometry

Wed Sep 07 00:00:00 EDT 2016

A Toll-like receptor 2 agonist-fused antigen enhanced antitumor immunity by increasing antigen presentation and the CD8 memory T cells population.

Oncotarget

Wu CC,Liu SJ,Chen HW,Shen KY,Leng CH

11-0271 was used in Flow cytometry/Cell sorting to explore the use of TLR2 agonist-fused antigens for efficient cancer therapy.

Tue May 24 00:00:00 EDT 2016

Production of IL-10 by CD4(+) regulatory T cells during the resolution of infection promotes the maturation of memory CD8(+) T cells.

Nature immunology

Laidlaw BJ,Cui W,Amezquita RA,Gray SM,Guan T,Lu Y,Kobayashi Y,Flavell RA,Kleinstein SH,Craft J,Kaech SM

11-0271 was used in Flow cytometry/Cell sorting to that Treg cell-derived IL-10 is needed to insulate, and promote the quality of, developing CD8(+) T cells.

Sat Aug 01 00:00:00 EDT 2015

Hyperreactive onchocerciasis is characterized by a combination of Th17-Th2 immune responses and reduced regulatory T cells.

PLoS neglected tropical diseases

Katawa G,Layland LE,Debrah AY,von Horn C,Batsa L,Kwarteng A,Arriens S,W Taylor D,Specht S,Hoerauf A,Adjobimey T

11-0271 was used in Flow cytometry/Cell sorting to provide initial evidence that elevated frequencies of Th17 and Th2 cells form part of the immune network instigating the development of severe onchocerciasis.

Thu Jan 01 00:00:00 EST 2015

Identification and characterization of latency-associated peptide-expressing γδ T cells.

Nature communications

Rezende RM,da Cunha AP,Kuhn C,Rubino S,M'Hamdi H,Gabriely G,Vandeventer T,Liu S,Cialic R,Pinheiro-Rosa N,Oliveira RP,Gaublomme JT,Obholzer N,Kozubek J,Pochet N,Faria AMC,Weiner HL

11-0271 was used in Flow cytometry/Cell sorting to describe a subset of regulatory γδ T cells that express the latency-associated peptide, a membrane-bound TGF-β1.

Tue Dec 08 00:00:00 EST 2015

Immunologic human renal allograft injury associates with an altered IL-10/TNF-α expression ratio in regulatory B cells.

Journal of the American Society of Nephrology : JASN

Cherukuri A,Rothstein DM,Clark B,Carter CR,Davison A,Hernandez-Fuentes M,Hewitt E,Salama AD,Baker RJ

11-0271 was used in Flow cytometry/Cell sorting to explore proinflammatory cytokine expression in B-cell subsets.

Tue Jul 01 00:00:00 EDT 2014

MHC-dependent inhibition of uterine NK cells impedes fetal growth and decidual vascular remodelling.

Nature communications

Kieckbusch J,Gaynor LM,Moffett A,Colucci F

11-0271 was used in Flow cytometry/Cell sorting to investigate MHC-dependent NK inhibition as a risk factor for pregnancy-related complications associated with impaired arterial remodelling.

Fri Feb 28 00:00:00 EST 2014

Bortezomib resistance can be reversed by induced expression of plasma cell maturation markers in a mouse in vitro model of multiple myeloma.

PloS one

Stessman HA,Mansoor A,Zhan F,Linden MA,Van Ness B,Baughn LB

11-0271 was used in Flow cytometry/Cell sorting to suggest a novel therapeutic approach for reversing Bz resistance in refractory multiple myeloma.

Tue Aug 05 00:00:00 EDT 2014

NK cells impede glioblastoma virotherapy through NKp30 and NKp46 natural cytotoxicity receptors.

Nature medicine

Alvarez-Breckenridge CA,Yu J,Price R,Wojton J,Pradarelli J,Mao H,Wei M,Wang Y,He S,Hardcastle J,Fernandez SA,Kaur B,Lawler SE,Vivier E,Mandelboim O,Moretta A,Caligiuri MA,Chiocca EA

11-0271 was used in Flow cytometry/Cell sorting to demonstrate that glioblastoma virotherapy is limited partially by an antiviral NK cell response involving specific NCRs, uncovering new potential targets to enhance cancer virotherapy.

Sat Dec 01 00:00:00 EST 2012

γδ T cells recognize a microbial encoded B cell antigen to initiate a rapid antigen-specific interleukin-17 response.

Immunity

Zeng X,Wei YL,Huang J,Newell EW,Yu H,Kidd BA,Kuhns MS,Waters RW,Davis MM,Weaver CT,Chien YH

11-0271 was used in Flow cytometry/Cell sorting to study the γδ T cell response to the algae protein, phycoerythrin.

Fri Sep 21 00:00:00 EDT 2012

Conditional IFNAR1 ablation reveals distinct requirements of Type I IFN signaling for NK cell maturation and tumor surveillance.

Oncoimmunology

Mizutani T,Neugebauer N,Putz EM,Moritz N,Simma O,Zebedin-Brandl E,Gotthardt D,Warsch W,Eckelhart E,Kantner HP,Kalinke U,Lienenklaus S,Weiss S,Strobl B,Müller M,Sexl V,Stoiber D

11-0271 was used in Flow cytometry/Cell sorting to examine the role of Type 1 IFN signalling in the prevention of leukaemogenesis.

Mon Oct 01 00:00:00 EDT 2012

Single-dose protection against Plasmodium berghei by a simian adenovirus vector using a human cytomegalovirus promoter containing intron A.

Journal of virology

Sridhar S,Reyes-Sandoval A,Draper SJ,Moore AC,Gilbert SC,Gao GP,Wilson JM,Hill AV

11-0271 was used in Flow cytometry/Cell sorting to compare efficacy of AdH5 and AdC6 vector vaccines expressing antigen under HCMV promotion.

Tue Apr 01 00:00:00 EDT 2008

Delayed, asynchronous, and reversible T-lineage specification induced by Notch/Delta signaling.

Genes & development

Taghon TN,David ES,Zúñiga-Pflücker JC,Rothenberg EV

11-0271 was used in Flow cytometry/Cell sorting to study how Notch/Delta signaling is not sufficient for T-lineage specification and commitment, but can be permissive for the maintenance and proliferation of uncommitted progenitors.

Fri Apr 15 00:00:00 EDT 2005

Characterization of murine CD70, the ligand of the TNF receptor family member CD27.

Journal of immunology (Baltimore, Md. : 1950)

Tesselaar K,Gravestein LA,van Schijndel GM,Borst J,van Lier RA

Sat Nov 15 00:00:00 EST 1997

Bioinformatics

Protein Aliases: CD antigen 27; CD27; CD27 antigen; CD27L receptor; LPFS2; sCD27; soluble CD27; T cell activation antigen S152; T-cell activation antigen CD27; T14; TNFSF7; Tumor necrosis factor receptor superfamily member 7; tumor necrosis factor receptor superfamily, member 7

View more View less

Gene Aliases: CD27; S152; S152. LPFS2; T14; TNFRSF7; Tp55

View more View less

UniProt ID: (Human) P26842, (Mouse) P41272

View more View less

Entrez Gene ID: (Human) 939, (Mouse) 21940, (Rat) 500318

View more View less

Function(s)

transmembrane signaling receptor activity tumor necrosis factor-activated receptor activity protein binding cysteine-type endopeptidase inhibitor activity involved in apoptotic process molecular_function

Process(es)

inflammatory response immune response cell surface receptor signaling pathway brain development release of cytoplasmic sequestered NF-kappaB extrinsic apoptotic signaling pathway via death domain receptors immunoglobulin mediated immune response response to lipopolysaccharide tumor necrosis factor-mediated signaling pathway regulation of cell proliferation negative regulation of apoptotic process negative regulation of cysteine-type endopeptidase activity involved in apoptotic process positive regulation of MAPK cascade response to ethanol positive regulation of B cell differentiation positive regulation of JNK cascade negative regulation of T cell apoptotic process extrinsic apoptotic signaling pathway apoptotic process B cell proliferation positive regulation of interferon-gamma biosynthetic process positive regulation of T cell differentiation immunoglobulin secretion

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

CD27 Monoclonal Antibody (LG.7F9), FITC, eBioscience™

CD27 Antibody (11-0271-82) in Flow

Product Details

11-0271-82

Flow Cytometry (Flow)

Functional Assay (Functional)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!