Invitrogen

CD27 Monoclonal Antibody (LG.7F9), Functional Grade, eBioscience™

FIGURE: 1 / 22

CD27 Antibody (16-0271-85) in Flow

Staining of BALB/c splenocytes with 0.25 µg Armenian Hamster IgG Isotype Control Purified (Product # 14-4888-81) (open histogram) or 0.25 µg Anti-CD27 Functional Grade Purified (filled histogram) followed by Anti-Armenian Hamster IgG FITC (Product # 11-4111).Total viable cells were analyzed.

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Product Details

16-0271-85

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.5 µg/test

View 23 publications 23 publications

Neutralization (Neu)

Assay-Dependent

Functional Assay (Functional)

Assay-Dependent

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse

Host/Isotype

Armenian hamster / IgG

Recommended Isotype Control

Armenian Hamster IgG Isotype Control (eBio299Arm), Functional Grade, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

LG.7F9

Conjugate

Functional Grade Functional Grade Functional Grade

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

no preservative

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_468917

Product Specific Information

Description: The LG.7F9 monoclonal antibody reacts with mouse CD27, a lymphocyte-specific member of the TNFR superfamily. CD27 is expressed by virtually all mature T cells and by a subpopulation of B cells, mainly memory B cells. In mouse, CD27 has been found on nearly all thymocytes excluding a population of CD46-CD8- precursors. CD27 binds to CD70 and, through this interaction, plays an important role in T cell-B cell interaction. It has been reported that triggering CD27 plays an important role in the maturation of CD4+ and CD8+ effector cells. LG.7F9 cross-reacts with human and rat CD27.

Applications Reported: The LG.7F9 antibody has been reported for use in flow cytometric analysis. LG.7F9 has also been reported in blocking of ligand binding.

Applications Tested: The LG.7F9 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Storage and handling: Use in a sterile environment.

Filtration: 0.2 µm post-manufacturing filtered.

Purity: Greater than 90%, as determined by SDS-PAGE.

Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.

Aggregation: Less than 10%, as determined by HPLC.

Target Information

CD27 is a 50 kDa member of the tumor necrosis factor (TNF) receptor superfamily that includes CD40 and CD30. The TNF superfamily members are known for the regulation of cell proliferation and death. In contrast to the expression of other TNFR/TNF family members, expression of CD27 and its ligand CD70 is predominantly confined to lymphocytes. High expression levels of CD27 appear to be dependent on proper ligation of antigen receptors. CD70 expression requires additional co-stimulatory and/or pro-inflammatory signals. CD27 is expressed as a disulfide-linked homodimer on mature thymocytes, peripheral blood T cells and a subpopulation of B cells. Activation of T cells via TCR-CD3 complex results in upregulation of CD27 expression on the plasma membrane as well as in the release of its soluble 28-32 kDa form, sCD27, detected in the plasma, urine or spinal fluid. Soluble CD27 is an important prognostic marker of acute and chronic B cell malignancies. RgpA, a cystein proteinase, although activating T cells through the protease-activated receptors (PARs), degradates CD27 and counteracts T cell activation mediated by CD27 and its ligand CD70. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor. Diseases associated with CD27 dysfunction include Lymphoproliferative Syndrome 2 and Autosomal Recessive Lymphoproliferative Syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Perinatal murine cytomegalovirus infection reshapes the transcriptional profile and functionality of NK cells.

Nature communications

Rožmanić C,Lisnić B,Pribanić Matešić M,Mihalić A,Hiršl L,Park E,Lesac Brizić A,Indenbirken D,Viduka I,Šantić M,Adler B,Yokoyama WM,Krmpotić A,Juranić Lisnić V,Jonjić S,Brizić I

Published figure using CD27 monoclonal antibody (Product # 16-0271-85) in Flow Cytometry

Thu Oct 12 00:00:00 EDT 2023

The transcription factor Bach2 negatively regulates murine natural killer cell maturation and function.

eLife

Li S,Bern MD,Miao B,Fan C,Xing X,Inoue T,Piersma SJ,Wang T,Colonna M,Kurosaki T,Yokoyama WM

Published figure using CD27 monoclonal antibody (Product # 16-0271-85) in Flow Cytometry

Mon Oct 03 00:00:00 EDT 2022

The transcriptional repressor ID2 supports natural killer cell maturation by controlling TCF1 amplitude.

The Journal of experimental medicine

Li ZY,Morman RE,Hegermiller E,Sun M,Bartom ET,Maienschein-Cline M,Sigvardsson M,Kee BL

Published figure using CD27 monoclonal antibody (Product # 16-0271-85) in Flow Cytometry

Mon Jun 07 00:00:00 EDT 2021

Chronic T cell proliferation in brains after stroke could interfere with the efficacy of immunotherapies.

The Journal of experimental medicine

Heindl S,Ricci A,Carofiglio O,Zhou Q,Arzberger T,Lenart N,Franzmeier N,Hortobagyi T,Nelson PT,Stowe AM,Denes A,Edbauer D,Liesz A

Published figure using CD27 monoclonal antibody (Product # 16-0271-85) in Flow Cytometry

Mon Aug 02 00:00:00 EDT 2021

PI3Kδ coordinates transcriptional, chromatin, and metabolic changes to promote effector CD8⁺ T cells at the expense of central memory.

Cell reports

Cannons JL,Villarino AV,Kapnick SM,Preite S,Shih HY,Gomez-Rodriguez J,Kaul Z,Shibata H,Reilley JM,Huang B,Handon R,McBain IT,Gossa S,Wu T,Su HC,McGavern DB,O'Shea JJ,McGuire PJ,Uzel G,Schwartzberg PL

Published figure using CD27 monoclonal antibody (Product # 16-0271-85) in Flow Cytometry

Tue Oct 12 00:00:00 EDT 2021

Effect of Subcutaneous Anti-CD20 Antibody-Mediated B Cell Depletion on Susceptibility to Pneumocystis Infection in Mice.

mSphere

Dai G,Noell K,Weckbecker G,Kolls JK

Published figure using CD27 monoclonal antibody (Product # 16-0271-85) in Flow Cytometry

Wed May 05 00:00:00 EDT 2021

Immune cell phenotypes associated with disease severity and long-term neutralizing antibody titers after natural dengue virus infection.

Cell reports. Medicine

Rouers A,Chng MHY,Lee B,Rajapakse MP,Kaur K,Toh YX,Sathiakumar D,Loy T,Thein TL,Lim VWX,Singhal A,Yeo TW,Leo YS,Vora KA,Casimiro D,Lim B,Tucker-Kellogg L,Rivino L,Newell EW,Fink K

Published figure using CD27 monoclonal antibody (Product # 16-0271-85) in Flow Cytometry

Tue May 18 00:00:00 EDT 2021

Development of a VRC01-class germline targeting immunogen derived from anti-idiotypic antibodies.

Cell reports

Seydoux E,Wan YH,Feng J,Wall A,Aljedani S,Homad LJ,MacCamy AJ,Weidle C,Gray MD,Brumage L,Taylor JJ,Pancera M,Stamatatos L,McGuire AT

Published figure using CD27 monoclonal antibody (Product # 16-0271-85) in Flow Cytometry

Tue May 04 00:00:00 EDT 2021

Ontogenic timing, T cell receptor signal strength, and Notch signaling direct γδ T cell functional differentiation in vivo.

Cell reports

Chen ELY,Lee CR,Thompson PK,Wiest DL,Anderson MK,Zúñiga-Pflücker JC

Published figure using CD27 monoclonal antibody (Product # 16-0271-85) in Flow Cytometry

Tue Jun 08 00:00:00 EDT 2021

Th2 Biased Immunity With Altered B Cell Profiles in Circulation of Patients With Sporotrichosis Caused by Sporothrix globosa.

Frontiers in immunology

Zu J,Yao L,Song Y,Cui Y,Guan M,Chen R,Zhen Y,Li S

Published figure using CD27 monoclonal antibody (Product # 16-0271-85) in Flow Cytometry

Mon May 31 00:00:00 EDT 2021

BALDR: a computational pipeline for paired heavy and light chain immunoglobulin reconstruction in single-cell RNA-seq data.

Genome medicine

Upadhyay AA,Kauffman RC,Wolabaugh AN,Cho A,Patel NB,Reiss SM,Havenar-Daughton C,Dawoud RA,Tharp GK,Sanz I,Pulendran B,Crotty S,Lee FE,Wrammert J,Bosinger SE

Published figure using CD27 monoclonal antibody (Product # 16-0271-85) in Flow Cytometry

Tue Mar 20 00:00:00 EDT 2018

Crosstalks between mTORC1 and mTORC2 variagate cytokine signaling to control NK maturation and effector function.

Nature communications

Wang F,Meng M,Mo B,Yang Y,Ji Y,Huang P,Lai W,Pan X,You T,Luo H,Guan X,Deng Y,Yuan S,Chu J,Namaka M,Hughes T,Ye L,Yu J,Li X,Deng Y

Published figure using CD27 monoclonal antibody (Product # 16-0271-85) in Flow Cytometry

Mon Nov 19 00:00:00 EST 2018

The breast tumor microenvironment alters the phenotype and function of natural killer cells.

Cellular & molecular immunology

Krneta T,Gillgrass A,Chew M,Ashkar AA

Published figure using CD27 monoclonal antibody (Product # 16-0271-85) in Flow Cytometry

Thu Sep 01 00:00:00 EDT 2016

NK cell development requires Tsc1-dependent negative regulation of IL-15-triggered mTORC1 activation.

Nature communications

Yang M,Chen S,Du J,He J,Wang Y,Li Z,Liu G,Peng W,Zeng X,Li D,Xu P,Guo W,Chang Z,Wang S,Tian Z,Dong Z

Published figure using CD27 monoclonal antibody (Product # 16-0271-85) in Flow Cytometry

Wed Sep 07 00:00:00 EDT 2016

Sirt6 regulates dendritic cell differentiation, maturation, and function.

Aging

Lasigliè D,Boero S,Bauer I,Morando S,Damonte P,Cea M,Monacelli F,Odetti P,Ballestrero A,Uccelli A,Mostoslavsky R,Poggi A,Nencioni A

16-0271 was used in Flow cytometry/Cell sorting to demonstrate that Sirt6KO mice have an altered frequency of cDCs and their precursors, altered receptor expression, and immunostimulatory capacity.

Fri Jan 01 00:00:00 EST 2016

Production of IL-10 by CD4(+) regulatory T cells during the resolution of infection promotes the maturation of memory CD8(+) T cells.

Nature immunology

Laidlaw BJ,Cui W,Amezquita RA,Gray SM,Guan T,Lu Y,Kobayashi Y,Flavell RA,Kleinstein SH,Craft J,Kaech SM

Published figure using CD27 monoclonal antibody (Product # 16-0271-85) in Flow Cytometry

Sat Aug 01 00:00:00 EDT 2015

Costimulation via the tumor-necrosis factor receptor superfamily couples TCR signal strength to the thymic differentiation of regulatory T cells.

Nature immunology

Mahmud SA,Manlove LS,Schmitz HM,Xing Y,Wang Y,Owen DL,Schenkel JM,Boomer JS,Green JM,Yagita H,Chi H,Hogquist KA,Farrar MA

16-0271 was used in Flow cytometry/Cell sorting to study the role of TNF receptors in the thymic development of Treg cells..

Thu May 01 00:00:00 EDT 2014

Tissue-resident natural killer (NK) cells are cell lineages distinct from thymic and conventional splenic NK cells.

eLife

Sojka DK,Plougastel-Douglas B,Yang L,Pak-Wittel MA,Artyomov MN,Ivanova Y,Zhong C,Chase JM,Rothman PB,Yu J,Riley JK,Zhu J,Tian Z,Yokoyama WM

16-0271 was used in Flow cytometry/Cell sorting to investigate whether tissue-resident and cells resembling conventional splenic natural killer cells natural killer cells are derived from the same lineage, showing that there are at least four distinct lineages.

Wed Jan 01 00:00:00 EST 2014

Pathogen-induced proapoptotic phenotype and high CD95 (Fas) expression accompany a suboptimal CD8+ T-cell response: reversal by adenoviral vaccine.

PLoS pathogens

Vasconcelos JR,Bruña-Romero O,Araújo AF,Dominguez MR,Ersching J,de Alencar BC,Machado AV,Gazzinelli RT,Bortoluci KR,Amarante-Mendes GP,Lopes MF,Rodrigues MM

16-0271 was used in Flow cytometry/Cell sorting to demonstrate that a suboptimal CD8+ T-cell response is associated with upregulation of CD95 expression and a proapoptotic phenotype.

Thu Nov 08 00:00:00 EST 2012

CD8 T cells recruited early in mouse polyomavirus infection undergo exhaustion.

Journal of immunology (Baltimore, Md. : 1950)

Wilson JJ,Pack CD,Lin E,Frost EL,Albrecht JA,Hadley A,Hofstetter AR,Tevethia SS,Schell TD,Lukacher AE

16-0271 was used in Flow cytometry/Cell sorting to characterise virus-specific CD8 T cells recruited during persistent infection.

Tue May 01 00:00:00 EDT 2012

The bone marrow functions as the central site of proliferation for long-lived NK cells.

Journal of immunology (Baltimore, Md. : 1950)

van Helden MJ,de Graaf N,Boog CJ,Topham DJ,Zaiss DM,Sijts AJ

16-0271 was used in Flow cytometry/Cell sorting to investigate the dynamics of natural killer cell responses after intranasal respiratory virus infection in mice.

Sat Sep 01 00:00:00 EDT 2012

STAT6 deletion converts the Th2 inflammatory pathology afflicting Lat(Y136F) mice into a lymphoproliferative disorder involving Th1 and CD8 effector T cells.

Journal of immunology (Baltimore, Md. : 1950)

Archambaud C,Sansoni A,Mingueneau M,Devilard E,Delsol G,Malissen B,Malissen M

16-0271 was used in Flow cytometry/Cell sorting to study the effect of STAT6 delection on Lat(Y136F) mice.

Sun Mar 01 00:00:00 EST 2009

T cell-independent development and induction of somatic hypermutation in human IgM+ IgD+ CD27+ B cells.

The Journal of experimental medicine

Scheeren FA,Nagasawa M,Weijer K,Cupedo T,Kirberg J,Legrand N,Spits H

16-0271 was used in Flow cytometry/Cell sorting to demonstrate that development and induction of SHMs of human IgM+IgD+CD27+ B cells can occur in a T cell-independent manner.

Mon Sep 01 00:00:00 EDT 2008

Characterization of murine CD70, the ligand of the TNF receptor family member CD27.

Journal of immunology (Baltimore, Md. : 1950)

Tesselaar K,Gravestein LA,van Schijndel GM,Borst J,van Lier RA

Sat Nov 15 00:00:00 EST 1997

Bioinformatics

Protein Aliases: CD antigen 27; CD27; CD27 antigen; CD27L receptor; LPFS2; sCD27; soluble CD27; T cell activation antigen S152; T-cell activation antigen CD27; T14; TNFSF7; Tumor necrosis factor receptor superfamily member 7; tumor necrosis factor receptor superfamily, member 7

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Gene Aliases: CD27; S152; S152. LPFS2; T14; TNFRSF7; Tp55

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UniProt ID: (Human) P26842, (Mouse) P41272

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Entrez Gene ID: (Human) 939, (Mouse) 21940, (Rat) 500318

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Function(s)

transmembrane signaling receptor activity tumor necrosis factor-activated receptor activity protein binding cysteine-type endopeptidase inhibitor activity involved in apoptotic process molecular_function

Process(es)

inflammatory response immune response cell surface receptor signaling pathway brain development release of cytoplasmic sequestered NF-kappaB extrinsic apoptotic signaling pathway via death domain receptors immunoglobulin mediated immune response response to lipopolysaccharide tumor necrosis factor-mediated signaling pathway regulation of cell proliferation negative regulation of apoptotic process negative regulation of cysteine-type endopeptidase activity involved in apoptotic process positive regulation of MAPK cascade response to ethanol positive regulation of B cell differentiation positive regulation of JNK cascade negative regulation of T cell apoptotic process extrinsic apoptotic signaling pathway apoptotic process B cell proliferation positive regulation of interferon-gamma biosynthetic process positive regulation of T cell differentiation immunoglobulin secretion

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD27 Monoclonal Antibody (LG.7F9), Functional Grade, eBioscience™

CD27 Antibody (16-0271-85) in Flow

Product Details

16-0271-85

Flow Cytometry (Flow)

Neutralization (Neu)

Functional Assay (Functional)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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