Invitrogen

CD27 Monoclonal Antibody (LG.7F9), APC-eFluor™ 780, eBioscience™

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Antibody Testing Data (1)
Published Figures (24)

FIGURE: 1 / 25

CD27 Antibody (47-0271-82) in Flow

Staining of C57Bl/6 splenocytes with Anti-Human/Mouse CD45R (B220) eFluor® 450 (Product # 48-0452-82) and 0.125 µg of Armenian Hamster IgG Isotype Control APC-eFluor® 780 (Product # 47-4888-80) (left) or 0.125 µg of Anti-CD27 APC-eFluor® 780 (right). Total viable cells were used for analysis.

Product Image Gallery

Product Details

47-0271-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.25 µg/test

View 22 publications 22 publications

Product Specifications

Species Reactivity

Rat, Mouse, Human

Published species

Human, Mouse

Host/Isotype

Armenian hamster / IgG

Recommended Isotype Control

Armenian Hamster IgG Isotype Control (eBio299Arm), APC-eFluor™ 780, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

LG.7F9

Conjugate

APC-eFluor™ 780 APC-eFluor™ 780 APC-eFluor™ 780

Excitation/Emission Max

756/785 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10853642

Product Specific Information

Description: The LG.7F9 monoclonal antibody reacts with mouse CD27, a lymphocyte-specific member of the TNFR superfamily. CD27 is expressed by virtually all mature T cells and by a subpopulation of B cells, mainly memory B cells. In mouse, CD27 has been found on nearly all thymocytes excluding a population of CD46-CD8- precursors. CD27 binds to CD70 and, through this interaction, plays an important role in T cell-B cell interaction. It has been reported that triggering CD27 plays an important role in the maturation of CD4+ and CD8+ effector cells. LG.7F9 cross-reacts with human and rat CD27.

Applications Reported: This LG.7F9 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This LG.7F9 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

APC-eFluor 780 emits at 780 nm and is excited with the Red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Light sensitivity: This tandem is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD27 is a 50 kDa member of the tumor necrosis factor (TNF) receptor superfamily that includes CD40 and CD30. The TNF superfamily members are known for the regulation of cell proliferation and death. In contrast to the expression of other TNFR/TNF family members, expression of CD27 and its ligand CD70 is predominantly confined to lymphocytes. High expression levels of CD27 appear to be dependent on proper ligation of antigen receptors. CD70 expression requires additional co-stimulatory and/or pro-inflammatory signals. CD27 is expressed as a disulfide-linked homodimer on mature thymocytes, peripheral blood T cells and a subpopulation of B cells. Activation of T cells via TCR-CD3 complex results in upregulation of CD27 expression on the plasma membrane as well as in the release of its soluble 28-32 kDa form, sCD27, detected in the plasma, urine or spinal fluid. Soluble CD27 is an important prognostic marker of acute and chronic B cell malignancies. RgpA, a cystein proteinase, although activating T cells through the protease-activated receptors (PARs), degradates CD27 and counteracts T cell activation mediated by CD27 and its ligand CD70. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor. Diseases associated with CD27 dysfunction include Lymphoproliferative Syndrome 2 and Autosomal Recessive Lymphoproliferative Syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

The transcription factor Bach2 negatively regulates murine natural killer cell maturation and function.

eLife

Li S,Bern MD,Miao B,Fan C,Xing X,Inoue T,Piersma SJ,Wang T,Colonna M,Kurosaki T,Yokoyama WM

Published figure using CD27 monoclonal antibody (Product # 47-0271-82) in Flow Cytometry

Mon Oct 03 00:00:00 EDT 2022

Transcriptomic Profiling of Plaque Psoriasis and Cutaneous T-Cell Subsets during Treatment with Secukinumab.

JID innovations : skin science from molecules to population health

Liu J,Chang HW,Grewal R,Cummins DD,Bui A,Beck KM,Sekhon S,Yan D,Huang ZM,Schmidt TH,Yang EJ,Sanchez IM,Nakamura M,Bhattarai S,Thibodeaux Q,Ahn R,Pauli M,Bhutani T,Rosenblum MD,Liao W

47-0271-82 was used in Flow cytometry/Cell sorting to reveal several phenotypic and cellular changes within the lesion that underlie clinical improvement from secukinumab.

Sun May 01 00:00:00 EDT 2022

The transcriptional repressor ID2 supports natural killer cell maturation by controlling TCF1 amplitude.

The Journal of experimental medicine

Li ZY,Morman RE,Hegermiller E,Sun M,Bartom ET,Maienschein-Cline M,Sigvardsson M,Kee BL

47-0271-82 was used in Flow Cytometry to demonstrate that ID2 sets a threshold for TCF1 during NK cell development, thus controlling the balance of immature and terminally differentiated cells that support future NK cell responses.

Mon Jun 07 00:00:00 EDT 2021

The transcriptional repressor ID2 supports natural killer cell maturation by controlling TCF1 amplitude.

The Journal of experimental medicine

Li ZY,Morman RE,Hegermiller E,Sun M,Bartom ET,Maienschein-Cline M,Sigvardsson M,Kee BL

Mon Jun 07 00:00:00 EDT 2021

Chronic T cell proliferation in brains after stroke could interfere with the efficacy of immunotherapies.

The Journal of experimental medicine

Heindl S,Ricci A,Carofiglio O,Zhou Q,Arzberger T,Lenart N,Franzmeier N,Hortobagyi T,Nelson PT,Stowe AM,Denes A,Edbauer D,Liesz A

Published figure using CD27 monoclonal antibody (Product # 47-0271-82) in Flow Cytometry

Mon Aug 02 00:00:00 EDT 2021

PI3Kδ coordinates transcriptional, chromatin, and metabolic changes to promote effector CD8⁺ T cells at the expense of central memory.

Cell reports

Cannons JL,Villarino AV,Kapnick SM,Preite S,Shih HY,Gomez-Rodriguez J,Kaul Z,Shibata H,Reilley JM,Huang B,Handon R,McBain IT,Gossa S,Wu T,Su HC,McGavern DB,O'Shea JJ,McGuire PJ,Uzel G,Schwartzberg PL

Published figure using CD27 monoclonal antibody (Product # 47-0271-82) in Flow Cytometry

Tue Oct 12 00:00:00 EDT 2021

Effect of Subcutaneous Anti-CD20 Antibody-Mediated B Cell Depletion on Susceptibility to Pneumocystis Infection in Mice.

mSphere

Dai G,Noell K,Weckbecker G,Kolls JK

Published figure using CD27 monoclonal antibody (Product # 47-0271-82) in Flow Cytometry

Wed May 05 00:00:00 EDT 2021

Immune cell phenotypes associated with disease severity and long-term neutralizing antibody titers after natural dengue virus infection.

Cell reports. Medicine

Rouers A,Chng MHY,Lee B,Rajapakse MP,Kaur K,Toh YX,Sathiakumar D,Loy T,Thein TL,Lim VWX,Singhal A,Yeo TW,Leo YS,Vora KA,Casimiro D,Lim B,Tucker-Kellogg L,Rivino L,Newell EW,Fink K

Published figure using CD27 monoclonal antibody (Product # 47-0271-82) in Flow Cytometry

Tue May 18 00:00:00 EDT 2021

Development of a VRC01-class germline targeting immunogen derived from anti-idiotypic antibodies.

Cell reports

Seydoux E,Wan YH,Feng J,Wall A,Aljedani S,Homad LJ,MacCamy AJ,Weidle C,Gray MD,Brumage L,Taylor JJ,Pancera M,Stamatatos L,McGuire AT

Published figure using CD27 monoclonal antibody (Product # 47-0271-82) in Flow Cytometry

Tue May 04 00:00:00 EDT 2021

Ontogenic timing, T cell receptor signal strength, and Notch signaling direct γδ T cell functional differentiation in vivo.

Cell reports

Chen ELY,Lee CR,Thompson PK,Wiest DL,Anderson MK,Zúñiga-Pflücker JC

47-0271-82 was used in Flow Cytometry to employ RBPJ-inducible and KN6-transgenic mice to assess the roles of ontogenic timing, T cell receptor (TCR) signal strength, and Notch signaling.

Tue Jun 08 00:00:00 EDT 2021

Th2 Biased Immunity With Altered B Cell Profiles in Circulation of Patients With Sporotrichosis Caused by Sporothrix globosa.

Frontiers in immunology

Zu J,Yao L,Song Y,Cui Y,Guan M,Chen R,Zhen Y,Li S

Published figure using CD27 monoclonal antibody (Product # 47-0271-82) in Flow Cytometry

Mon May 31 00:00:00 EDT 2021

Hhex Directly Represses BIM-Dependent Apoptosis to Promote NK Cell Development and Maintenance.

Cell reports

Goh W,Scheer S,Jackson JT,Hediyeh-Zadeh S,Delconte RB,Schuster IS,Andoniou CE,Rautela J,Degli-Esposti MA,Davis MJ,McCormack MP,Nutt SL,Huntington ND

47-0271 was used in Flow cytometry/Cell sorting to show homeobox transcriptional regulator Hhex regulates NK cell homeostasis and immunity.

Tue Oct 20 00:00:00 EDT 2020

BALDR: a computational pipeline for paired heavy and light chain immunoglobulin reconstruction in single-cell RNA-seq data.

Genome medicine

Upadhyay AA,Kauffman RC,Wolabaugh AN,Cho A,Patel NB,Reiss SM,Havenar-Daughton C,Dawoud RA,Tharp GK,Sanz I,Pulendran B,Crotty S,Lee FE,Wrammert J,Bosinger SE

Published figure using CD27 monoclonal antibody (Product # 47-0271-82) in Flow Cytometry

Tue Mar 20 00:00:00 EDT 2018

Crosstalks between mTORC1 and mTORC2 variagate cytokine signaling to control NK maturation and effector function.

Nature communications

Wang F,Meng M,Mo B,Yang Y,Ji Y,Huang P,Lai W,Pan X,You T,Luo H,Guan X,Deng Y,Yuan S,Chu J,Namaka M,Hughes T,Ye L,Yu J,Li X,Deng Y

Published figure using CD27 monoclonal antibody (Product # 47-0271-82) in Flow Cytometry

Mon Nov 19 00:00:00 EST 2018

Stabilization of cytokine mRNAs in iNKT cells requires the serine-threonine kinase IRE1alpha.

Nature communications

Govindarajan S,Gaublomme D,Van der Cruyssen R,Verheugen E,Van Gassen S,Saeys Y,Tavernier S,Iwawaki T,Bloch Y,Savvides SN,Lambrecht BN,Janssens S,Elewaut D,Drennan MB

47-0271 was used in Flow cytometry/Cell sorting to examine the role of the IRE1α-dependent signalling cascade in coupling constitutive cytokine mRNA expression to the rapid induction of cytokine secretion and effector functions in activated iNKT cells.

Mon Dec 17 00:00:00 EST 2018

Differentiation and Protective Capacity of Virus-Specific CD8⁺ T Cells Suggest Murine Norovirus Persistence in an Immune-Privileged Enteric Niche.

Immunity

Tomov VT,Palko O,Lau CW,Pattekar A,Sun Y,Tacheva R,Bengsch B,Manne S,Cosma GL,Eisenlohr LC,Nice TJ,Virgin HW,Wherry EJ

47-0271 was used in Flow cytometry/Cell sorting to elucidate a strategy of immune evasion by mouse norovirus via the induction of a CD8+ T cell program normally reserved for latent pathogens and persistence in an immune-privileged enteric niche.

Tue Oct 17 00:00:00 EDT 2017

Single-cell tracking of flavivirus RNA uncovers species-specific interactions with the immune system dictating disease outcome.

Nature communications

Douam F,Hrebikova G,Albrecht YE,Sellau J,Sharon Y,Ding Q,Ploss A

47-0271 was used in Flow cytometry/Cell sorting to demonstrate the tracking of and sense viral RNA at single-cell resolution within complex subsets of the human and murine immune system in different mouse models.

Tue Mar 14 00:00:00 EDT 2017

Type III Interferon-Mediated Signaling Is Critical for Controlling Live Attenuated Yellow Fever Virus Infection In Vivo.

mBio

Douam F,Soto Albrecht YE,Hrebikova G,Sadimin E,Davidson C,Kotenko SV,Ploss A

Tue Aug 15 00:00:00 EDT 2017

The breast tumor microenvironment alters the phenotype and function of natural killer cells.

Cellular & molecular immunology

Krneta T,Gillgrass A,Chew M,Ashkar AA

Published figure using CD27 monoclonal antibody (Product # 47-0271-82) in Flow Cytometry

Thu Sep 01 00:00:00 EDT 2016

NK cell development requires Tsc1-dependent negative regulation of IL-15-triggered mTORC1 activation.

Nature communications

Yang M,Chen S,Du J,He J,Wang Y,Li Z,Liu G,Peng W,Zeng X,Li D,Xu P,Guo W,Chang Z,Wang S,Tian Z,Dong Z

Published figure using CD27 monoclonal antibody (Product # 47-0271-82) in Flow Cytometry

Wed Sep 07 00:00:00 EDT 2016

Production of IL-10 by CD4(+) regulatory T cells during the resolution of infection promotes the maturation of memory CD8(+) T cells.

Nature immunology

Laidlaw BJ,Cui W,Amezquita RA,Gray SM,Guan T,Lu Y,Kobayashi Y,Flavell RA,Kleinstein SH,Craft J,Kaech SM

Published figure using CD27 monoclonal antibody (Product # 47-0271-82) in Flow Cytometry

Sat Aug 01 00:00:00 EDT 2015

CD24(hi)CD27⁺ and plasmablast-like regulatory B cells in human chronic graft-versus-host disease.

Blood

de Masson A,Bouaziz JD,Le Buanec H,Robin M,O'Meara A,Parquet N,Rybojad M,Hau E,Monfort JB,Branchtein M,Michonneau D,Dessirier V,Sicre de Fontbrune F,Bergeron A,Itzykson R,Dhédin N,Bengoufa D,Peffault de Latour R,Xhaard A,Bagot M,Bensussan A,Socié G

47-0271 was used in Flow cytometry/Cell sorting to suggest a role of CD24(hi)CD27(+) B-cell and plasmablast-derived IL-10 in the regulation of human chronic graft-versus-host disease (cGVHD).

Thu Mar 12 00:00:00 EDT 2015

PD-1+Tim-3+ CD8+ T Lymphocytes Display Varied Degrees of Functional Exhaustion in Patients with Regionally Metastatic Differentiated Thyroid Cancer.

Cancer immunology research

Severson JJ,Serracino HS,Mateescu V,Raeburn CD,McIntyre RC,Sams SB,Haugen BR,French JD

47-0271 was used in Flow cytometry/Cell sorting to determine that the release of PD-1 and Tim-3, and the inhibition of Tregs, may encourage tumour elimination in metastatic differentiated thyroid cancer.

Mon Jun 01 00:00:00 EDT 2015

Bioinformatics

Protein Aliases: CD antigen 27; CD27; CD27 antigen; CD27L receptor; LPFS2; sCD27; soluble CD27; T cell activation antigen S152; T-cell activation antigen CD27; T14; TNFSF7; Tumor necrosis factor receptor superfamily member 7; tumor necrosis factor receptor superfamily, member 7

View more View less

Gene Aliases: CD27; S152; S152. LPFS2; T14; TNFRSF7; Tp55

View more View less

UniProt ID: (Human) P26842, (Mouse) P41272

View more View less

Entrez Gene ID: (Human) 939, (Mouse) 21940, (Rat) 500318

View more View less

Function(s)

transmembrane signaling receptor activity tumor necrosis factor-activated receptor activity protein binding cysteine-type endopeptidase inhibitor activity involved in apoptotic process molecular_function

Process(es)

inflammatory response immune response cell surface receptor signaling pathway brain development release of cytoplasmic sequestered NF-kappaB extrinsic apoptotic signaling pathway via death domain receptors immunoglobulin mediated immune response response to lipopolysaccharide tumor necrosis factor-mediated signaling pathway regulation of cell proliferation negative regulation of apoptotic process negative regulation of cysteine-type endopeptidase activity involved in apoptotic process positive regulation of MAPK cascade response to ethanol positive regulation of B cell differentiation positive regulation of JNK cascade negative regulation of T cell apoptotic process extrinsic apoptotic signaling pathway apoptotic process B cell proliferation positive regulation of interferon-gamma biosynthetic process positive regulation of T cell differentiation immunoglobulin secretion

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

CD27 Monoclonal Antibody (LG.7F9), APC-eFluor™ 780, eBioscience™

CD27 Antibody (47-0271-82) in Flow

Product Details

47-0271-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!