Invitrogen

CD28 Monoclonal Antibody (CD28.2), FITC, eBioscience™

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CD28 Antibody (11-0289-42) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD19 APC (Product # 17-0199-42) and Mouse IgG1 K Isotype Control FITC (Product # 11-4714-42) (left) or Anti-Human CD28 FITC (right). Cells in the lymphocyte gate were used for analysis.

CD28 Antibody in T-Cell Activation (TCA)

CD28 Antibody in Functional Assay (Functional)

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Product Details

11-0289-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (1 µg)/test

View 20 publications 20 publications

Functional Assay (Functional)

View 2 publications 2 publications

T-Cell Activation (TCA)

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), FITC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

CD28.2

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10733610

Product Specific Information

Description: The CD28.2 monoclonal antibody reacts with the human CD28 molecule, a 44 kDa homodimer expressed by thymocytes, mature T cells and plasma cells. CD28 is a ligand for CD80 (B7-1) and CD86 (B7-2) and is a potent co-stimulator of T cells. Signaling through CD28 augments IL-2 and IL-2 receptor expression as well as cytotoxicity of CD3-activated T cells.

Applications Reported: The CD28.2 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This CD28.2 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (1 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD28 antigen is a 44 kDa disulfide linked homodimeric T cell specific surface glycoprotein. CD28 is a cell adhesion molecule of the immunoglobulin superfamily which is constitutively expressed on most peripheral blood T lymphocytes. Moreover, CD28 is the critical T cell costimulatory receptor that provides the cell the important second activation signal by binding CD80 and CD86 which are expressed by antigen presenting cells. In addition to its co-stimulation role, CD28 functions by preventing T cells from entering an anergic-hyporesponsive state or from undergoing premature apoptotic cell death. In murine peripheral lymphoid organs and in the blood, all CD4+ and CD8+ T cells express CD28. In the thymus, CD28 expression is highest on immature CD3-, CD8+ and CD4+8+ cells, and on CD4-8- cells that express alpha/beta and gamma/delta TCR. The level of CD28 on mature CD4+ and CD8+ alpha/beta TCR+ thymocytes is two- to fourfold lower than on the immature cells. Diseases associated with CD28 dysfunction include mycosis fungiodes and Sezary's Disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Microbially-Induced Exosomes from Dendritic Cells Promote Paracrine Immune Senescence: Novel Mechanism of Bone Degenerative Disease in Mice.

Aging and disease

Elsayed R,Elashiry M,Liu Y,Morandini AC,El-Awady A,Elashiry MM,Hamrick M,Cutler CW

11-0289-42 was used in Flow cytometry/Cell sorting to conclude that immune senescence, compounded by advanced age, and accelerated by oral dysbiosis and its induced SASP exosomes, plays a pivotal role in the pathophysiology of experimental periodontitis.

Wed Feb 01 00:00:00 EST 2023

Engineered Human Dendritic Cell Exosomes as Effective Delivery System for Immune Modulation.

International journal of molecular sciences

Elsayed R,Elashiry M,Tran C,Yang T,Carroll A,Liu Y,Hamrick M,Cutler CW

11-0289-42 was used in Flow cytometry/Cell sorting to report in a mouse model of periodontitis that custom murine DC-derived exo subtypes could reprogram the immune response toward a bone-sparing or bone-loss phenotype, depending on immune profile.

Tue Jul 11 00:00:00 EDT 2023

CHI3L1 enhances melanoma lung metastasis via regulation of T cell co-stimulators and CTLA-4/B7 axis.

Frontiers in immunology

Ma B,Kamle S,Akosman B,Khan H,Lee CM,Lee CG,Elias JA

Published figure using CD28 monoclonal antibody (Product # 11-0289-42) in Flow Cytometry

Tue Jan 10 00:00:00 EST 2023

SNX9-induced membrane tubulation regulates CD28 cluster stability and signalling.

eLife

Ecker M,Schregle R,Kapoor-Kaushik N,Rossatti P,Betzler VM,Kempe D,Biro M,Ariotti N,Redpath GM,Rossy J

11-0289-42 was used in Flow cytometry/Cell sorting to suggest a model in which SNX9-mediated tubulation generates a membrane environment that promotes CD28 triggering and downstream signalling events.

Thu Jan 20 00:00:00 EST 2022

Longitudinal profiling of respiratory and systemic immune responses reveals myeloid cell-driven lung inflammation in severe COVID-19.

Immunity

Szabo PA,Dogra P,Gray JI,Wells SB,Connors TJ,Weisberg SP,Krupska I,Matsumoto R,Poon MML,Idzikowski E,Morris SE,Pasin C,Yates AJ,Ku A,Chait M,Davis-Porada J,Guo XV,Zhou J,Steinle M,Mackay S,Saqi A,Baldwin MR,Sims PA,Farber DL

Published figure using CD28 monoclonal antibody (Product # 11-0289-42) in Flow Cytometry

Tue Apr 13 00:00:00 EDT 2021

Effects of the Antidiabetic Drugs Evogliptin and Sitagliptin on the Immune Function of CD26/DPP4 in Th1 Cells.

Biomolecules & therapeutics

Yoon H,Sung JH,Song MJ

Published figure using CD28 monoclonal antibody (Product # 11-0289-42) in Flow Cytometry

Mon Mar 01 00:00:00 EST 2021

CD19 Chimeric Antigen Receptor-Exosome Targets CD19 Positive B-lineage Acute Lymphocytic Leukemia and Induces Cytotoxicity.

Cancers

Haque S,Vaiselbuh SR

Published figure using CD28 monoclonal antibody (Product # 11-0289-42) in Flow Cytometry

Fri Mar 19 00:00:00 EDT 2021

Differences in Maturation Status and Immune Phenotypes of Circulating Helios⁺ and Helios^- Tregs and Their Disrupted Correlations With Monocyte Subsets in Autoantibody-Positive T1D Individuals.

Frontiers in immunology

Zhang Y,Zhang J,Shi Y,Shen M,Lv H,Chen S,Feng Y,Chen H,Xu X,Yang T,Xu K

Published figure using CD28 monoclonal antibody (Product # 11-0289-42) in Flow Cytometry

Wed Sep 15 00:00:00 EDT 2021

SIRT1 is downregulated by autophagy in senescence and ageing.

Nature cell biology

Xu C,Wang L,Fozouni P,Evjen G,Chandra V,Jiang J,Lu C,Nicastri M,Bretz C,Winkler JD,Amaravadi R,Garcia BA,Adams PD,Ott M,Tong W,Johansen T,Dou Z,Berger SL

Published figure using CD28 monoclonal antibody (Product # 11-0289-42) in Flow Cytometry

Thu Oct 01 00:00:00 EDT 2020

PD-1 and BTLA regulate T cell signaling differentially and only partially through SHP1 and SHP2.

The Journal of cell biology

Xu X,Hou B,Fulzele A,Masubuchi T,Zhao Y,Wu Z,Hu Y,Jiang Y,Ma Y,Wang H,Bennett EJ,Fu G,Hui E

Published figure using CD28 monoclonal antibody (Product # 11-0289-42) in Flow Cytometry

Mon Jun 01 00:00:00 EDT 2020

Expression of CD27 and CD28 on γδ T cells from the peripheral blood of patients with allergic rhinitis.

Experimental and therapeutic medicine

Wang Q,Sun Q,Chen Q,Li H,Liu D

Published figure using CD28 monoclonal antibody (Product # 11-0289-42) in Flow Cytometry

Tue Dec 01 00:00:00 EST 2020

Human Liver Memory CD8⁺ T Cells Use Autophagy for Tissue Residence.

Cell reports

Swadling L,Pallett LJ,Diniz MO,Baker JM,Amin OE,Stegmann KA,Burton AR,Schmidt NM,Jeffery-Smith A,Zakeri N,Suveizdyte K,Froghi F,Fusai G,Rosenberg WM,Davidson BR,Schurich A,Simon AK,Maini MK

Published figure using CD28 monoclonal antibody (Product # 11-0289-42) in T-Cell Activation

Tue Jan 21 00:00:00 EST 2020

Selective Killing of Activated T Cells by 5-Aminolevulinic Acid Mediated Photodynamic Effect: Potential Improvement of Extracorporeal Photopheresis.

Cancers

Darvekar S,Juzenas P,Oksvold M,Kleinauskas A,Holien T,Christensen E,Stokke T,Sioud M,Peng Q

Published figure using CD28 monoclonal antibody (Product # 11-0289-42) in Flow Cytometry

Thu Feb 06 00:00:00 EST 2020

RAB11FIP5-Deficient Mice Exhibit Cytokine-Related Transcriptomic Signatures.

ImmunoHorizons

Li D,Bradley T,Cain DW,Pedroza-Pacheco I,Aggelakopoulou M,Parks R,Barr M,Xia SM,Scearce R,Bowman C,Stevens G,Newman A,Hora B,Chen Y,Riebe K,Wang Y,Sempowski G,Saunders KO,Borrow P,Haynes BF

Published figure using CD28 monoclonal antibody (Product # 11-0289-42) in Functional assay

Tue Nov 10 00:00:00 EST 2020

Metabolic reprogramming of human CD8⁺ memory T cells through loss of SIRT1.

The Journal of experimental medicine

Jeng MY,Hull PA,Fei M,Kwon HS,Tsou CL,Kasler H,Ng CP,Gordon DE,Johnson J,Krogan N,Verdin E,Ott M

Published figure using CD28 monoclonal antibody (Product # 11-0289-42) in Flow Cytometry

Tue Jan 02 00:00:00 EST 2018

Staphylococcal Superantigens Use LAMA2 as a Coreceptor To Activate T Cells.

Journal of immunology (Baltimore, Md. : 1950)

Li Z,Zeppa JJ,Hancock MA,McCormick JK,Doherty TM,Hendy GN,Madrenas J

11-0289 was used in Flow cytometry/Cell sorting to identify the α2 subunit of the extracellular matrix protein laminin(LAMA2) acts as the coreceptor for staphylococcal superantigens.

Thu Feb 15 00:00:00 EST 2018

The Distribution of Human Stem Cell-like Memory T Cell in Lung Cancer.

Journal of immunotherapy (Hagerstown, Md. : 1997)

Hong H,Gu Y,Sheng SY,Lu CG,Zou JY,Wu CY

Published figure using CD28 monoclonal antibody (Product # 11-0289-42) in Flow Cytometry

Thu Jan 04 00:00:00 EST 2018

CD28 family of receptors on T cells in chronic HBV infection: Expression characteristics, clinical significance and correlations with PD-1 blockade.

Molecular medicine reports

Tang ZS,Hao YH,Zhang EJ,Xu CL,Zhou Y,Zheng X,Yang DL

Published figure using CD28 monoclonal antibody (Product # 11-0289-42) in Flow Cytometry

Mon Aug 01 00:00:00 EDT 2016

Protective effects of SIRT1 in patients with proliferative diabetic retinopathy via the inhibition of IL-17 expression.

Experimental and therapeutic medicine

Liu S,Lin YU,Liu X

11-0289 was used in an ELISA assay to investigate the protective effects of SIRT1 in patients with proliferative diabetic retinopathy via the inhibition of IL-17 expression.

Fri Jan 01 00:00:00 EST 2016

Aging and cytomegalovirus infection differentially and jointly affect distinct circulating T cell subsets in humans.

Journal of immunology (Baltimore, Md. : 1950)

Wertheimer AM,Bennett MS,Park B,Uhrlaub JL,Martinez C,Pulko V,Currier NL,Nikolich-Žugich D,Kaye J,Nikolich-Žugich J

Published figure using CD28 monoclonal antibody (Product # 11-0289-42) in Flow Cytometry

Sat Mar 01 00:00:00 EST 2014

N-glycosylation of asparagine 8 regulates surface expression of major histocompatibility complex class I chain-related protein A (MICA) alleles dependent on threonine 24.

The Journal of biological chemistry

Mellergaard M,Skovbakke SL,Schneider CL,Lauridsen F,Andresen L,Jensen H,Skov S

Published figure using CD28 monoclonal antibody (Product # 11-0289-42) in T-Cell Activation

Fri Jul 18 00:00:00 EDT 2014

Detailed characterization of tumor infiltrating lymphocytes in two distinct human solid malignancies show phenotypic similarities.

Journal for immunotherapy of cancer

Kovacsovics-Bankowski M,Chisholm L,Vercellini J,Tucker CG,Montler R,Haley D,Newell P,Ma J,Tseng P,Wolf R,Vetto JT,Hammill C,Hansen P,Weinberg AD

Published figure using CD28 monoclonal antibody (Product # 11-0289-42) in Flow Cytometry

Mon Dec 01 00:00:00 EST 2014

A novel method for autophagy detection in primary cells: impaired levels of macroautophagy in immunosenescent T cells.

Autophagy

Phadwal K,Alegre-Abarrategui J,Watson AS,Pike L,Anbalagan S,Hammond EM,Wade-Martins R,McMichael A,Klenerman P,Simon AK

Published figure using CD28 monoclonal antibody (Product # 11-0289-42) in Flow Cytometry

Sun Apr 01 00:00:00 EDT 2012

B7-CD28 costimulatory signals control the survival and proliferation of murine and human γδ T cells via IL-2 production.

Journal of immunology (Baltimore, Md. : 1950)

Ribot JC,Debarros A,Mancio-Silva L,Pamplona A,Silva-Santos B

11-0289 was used in Flow cytometry/Cell sorting to show that CD28 signals are required for IL-2-mediated survival and proliferation of both CD27(+) and CD27(-) γδ T cell subsets.

Wed Aug 01 00:00:00 EDT 2012

Phosphorylation of Bcl10 negatively regulates T-cell receptor-mediated NF-kappaB activation.

Molecular and cellular biology

Zeng H,Di L,Fu G,Chen Y,Gao X,Xu L,Lin X,Wen R

Published figure using CD28 monoclonal antibody (Product # 11-0289-42) in T-Cell Activation

Sun Jul 01 00:00:00 EDT 2007

Bioinformatics

Protein Aliases: CD28; CD28 antigen; MGC138290; sCD28; soluble CD28; T-cell-specific surface glycoprotein CD28; T44; TP44

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Gene Aliases: CD28; Tp44

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UniProt ID: (Human) P10747

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Entrez Gene ID: (Human) 940

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Function(s)

protease binding SH3/SH2 adaptor activity protein binding coreceptor activity protein kinase binding identical protein binding phosphatidylinositol-4,5-bisphosphate 3-kinase activity immunoglobulin receptor superfamily

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD28 Monoclonal Antibody (CD28.2), FITC, eBioscience™

CD28 Antibody (11-0289-42) in Flow

Product Details

11-0289-42

Flow Cytometry (Flow)

Functional Assay (Functional)

T-Cell Activation (TCA)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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