Invitrogen

CD28 Monoclonal Antibody (CD28.2), PerCP-Cyanine5.5, eBioscience™

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CD28 Antibody (45-0289-42) in Flow

Normal human peripheral blood cells were stained with CD19 Monoclonal Antibody, FITC (Product # 11-0199-42) and Mouse IgG1 kappa Isotype Control, PerCP-Cyanine5.5 (Product # 45-4714-82) (left) or CD28 Monoclonal Antibody, PerCP-Cyanine5.5 (right). Cells in the lymphocyte gate were used for analysis.

Product Image Gallery

Product Details

45-0289-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.125 µg)/test

View 22 publications 22 publications

Product Specifications

Species Reactivity

Human

Published species

Cynomolgus monkey, Human, Rhesus monkey

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PerCP-Cyanine5.5, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

CD28.2

Conjugate

PerCP-Cyanine5.5 PerCP-Cyanine5.5 PerCP-Cyanine5.5

Excitation/Emission Max

489/679 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10714974

Product Specific Information

Description: The CD28.2 monoclonal antibody reacts with the human CD28 molecule, a 44 kDa homodimer expressed by thymocytes, mature T cells and plasma cells. CD28 is a ligand for CD80 (B7-1) and CD86 (B7-2) and is a potent co-stimulator of T cells. Signaling through CD28 augments IL-2 and IL-2 receptor expression as well as cytotoxicity of CD3-activated T cells.

Applications Reported: This CD28.2 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This CD28.2 antibody has been pre-titrated and tested by flow cytometric analysi sof normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488 nm; Emission: 695 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD28 antigen is a 44 kDa disulfide linked homodimeric T cell specific surface glycoprotein. CD28 is a cell adhesion molecule of the immunoglobulin superfamily which is constitutively expressed on most peripheral blood T lymphocytes. Moreover, CD28 is the critical T cell costimulatory receptor that provides the cell the important second activation signal by binding CD80 and CD86 which are expressed by antigen presenting cells. In addition to its co-stimulation role, CD28 functions by preventing T cells from entering an anergic-hyporesponsive state or from undergoing premature apoptotic cell death. In murine peripheral lymphoid organs and in the blood, all CD4+ and CD8+ T cells express CD28. In the thymus, CD28 expression is highest on immature CD3-, CD8+ and CD4+8+ cells, and on CD4-8- cells that express alpha/beta and gamma/delta TCR. The level of CD28 on mature CD4+ and CD8+ alpha/beta TCR+ thymocytes is two- to fourfold lower than on the immature cells. Diseases associated with CD28 dysfunction include mycosis fungiodes and Sezary's Disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

CHI3L1 enhances melanoma lung metastasis via regulation of T cell co-stimulators and CTLA-4/B7 axis.

Frontiers in immunology

Ma B,Kamle S,Akosman B,Khan H,Lee CM,Lee CG,Elias JA

Published figure using CD28 monoclonal antibody (Product # 45-0289-42) in Flow Cytometry

Tue Jan 10 00:00:00 EST 2023

Longitudinal profiling of respiratory and systemic immune responses reveals myeloid cell-driven lung inflammation in severe COVID-19.

Immunity

Szabo PA,Dogra P,Gray JI,Wells SB,Connors TJ,Weisberg SP,Krupska I,Matsumoto R,Poon MML,Idzikowski E,Morris SE,Pasin C,Yates AJ,Ku A,Chait M,Davis-Porada J,Guo XV,Zhou J,Steinle M,Mackay S,Saqi A,Baldwin MR,Sims PA,Farber DL

Published figure using CD28 monoclonal antibody (Product # 45-0289-42) in Flow Cytometry

Tue Apr 13 00:00:00 EDT 2021

Effects of the Antidiabetic Drugs Evogliptin and Sitagliptin on the Immune Function of CD26/DPP4 in Th1 Cells.

Biomolecules & therapeutics

Yoon H,Sung JH,Song MJ

Published figure using CD28 monoclonal antibody (Product # 45-0289-42) in Flow Cytometry

Mon Mar 01 00:00:00 EST 2021

CD19 Chimeric Antigen Receptor-Exosome Targets CD19 Positive B-lineage Acute Lymphocytic Leukemia and Induces Cytotoxicity.

Cancers

Haque S,Vaiselbuh SR

Published figure using CD28 monoclonal antibody (Product # 45-0289-42) in Flow Cytometry

Fri Mar 19 00:00:00 EDT 2021

Differences in Maturation Status and Immune Phenotypes of Circulating Helios⁺ and Helios^- Tregs and Their Disrupted Correlations With Monocyte Subsets in Autoantibody-Positive T1D Individuals.

Frontiers in immunology

Zhang Y,Zhang J,Shi Y,Shen M,Lv H,Chen S,Feng Y,Chen H,Xu X,Yang T,Xu K

Published figure using CD28 monoclonal antibody (Product # 45-0289-42) in Flow Cytometry

Wed Sep 15 00:00:00 EDT 2021

SIRT1 is downregulated by autophagy in senescence and ageing.

Nature cell biology

Xu C,Wang L,Fozouni P,Evjen G,Chandra V,Jiang J,Lu C,Nicastri M,Bretz C,Winkler JD,Amaravadi R,Garcia BA,Adams PD,Ott M,Tong W,Johansen T,Dou Z,Berger SL

Published figure using CD28 monoclonal antibody (Product # 45-0289-42) in Flow Cytometry

Thu Oct 01 00:00:00 EDT 2020

PD-1 and BTLA regulate T cell signaling differentially and only partially through SHP1 and SHP2.

The Journal of cell biology

Xu X,Hou B,Fulzele A,Masubuchi T,Zhao Y,Wu Z,Hu Y,Jiang Y,Ma Y,Wang H,Bennett EJ,Fu G,Hui E

Published figure using CD28 monoclonal antibody (Product # 45-0289-42) in Flow Cytometry

Mon Jun 01 00:00:00 EDT 2020

Expression of CD27 and CD28 on γδ T cells from the peripheral blood of patients with allergic rhinitis.

Experimental and therapeutic medicine

Wang Q,Sun Q,Chen Q,Li H,Liu D

Published figure using CD28 monoclonal antibody (Product # 45-0289-42) in Flow Cytometry

Tue Dec 01 00:00:00 EST 2020

Selective Killing of Activated T Cells by 5-Aminolevulinic Acid Mediated Photodynamic Effect: Potential Improvement of Extracorporeal Photopheresis.

Cancers

Darvekar S,Juzenas P,Oksvold M,Kleinauskas A,Holien T,Christensen E,Stokke T,Sioud M,Peng Q

Published figure using CD28 monoclonal antibody (Product # 45-0289-42) in Flow Cytometry

Thu Feb 06 00:00:00 EST 2020

Metabolic reprogramming of human CD8⁺ memory T cells through loss of SIRT1.

The Journal of experimental medicine

Jeng MY,Hull PA,Fei M,Kwon HS,Tsou CL,Kasler H,Ng CP,Gordon DE,Johnson J,Krogan N,Verdin E,Ott M

Published figure using CD28 monoclonal antibody (Product # 45-0289-42) in Flow Cytometry

Tue Jan 02 00:00:00 EST 2018

A randomized control trial to establish the feasibility and safety of rapamycin treatment in an older human cohort: Immunological, physical performance, and cognitive effects.

Experimental gerontology

Kraig E,Linehan LA,Liang H,Romo TQ,Liu Q,Wu Y,Benavides AD,Curiel TJ,Javors MA,Musi N,Chiodo L,Koek W,Gelfond JAL,Kellogg DL

45-0289-42 was used in Flow cytometry/Cell sorting to demonstrate that short-term RAPA treatment appeared safe, with no significant adverse effects on metabolism, cognitive function, physical performance, or immune parameters.

Tue May 01 00:00:00 EDT 2018

Surrogate in vitro activation of innate immunity synergizes with interleukin-7 to unleash rapid antigen-driven outgrowth of CD4+ and CD8+ human peripheral blood T-cells naturally recognizing MUC1, HER2/neu and other tumor-associated antigens.

Oncotarget

Pathangey LB,McCurry DB,Gendler SJ,Dominguez AL,Gorman JE,Pathangey G,Mihalik LA,Dang Y,Disis ML,Cohen PA

45-0289 was used in Flow cytometry/Cell sorting to create a mechanistically rational culture sequence, enabling rapid preparation of T-cells recognizing tumor-associated antigens expressed by the majority of human cancer.

Tue Feb 14 00:00:00 EST 2017

The Distribution of Human Stem Cell-like Memory T Cell in Lung Cancer.

Journal of immunotherapy (Hagerstown, Md. : 1997)

Hong H,Gu Y,Sheng SY,Lu CG,Zou JY,Wu CY

Published figure using CD28 monoclonal antibody (Product # 45-0289-42) in Flow Cytometry

Thu Jan 04 00:00:00 EST 2018

CD28 family of receptors on T cells in chronic HBV infection: Expression characteristics, clinical significance and correlations with PD-1 blockade.

Molecular medicine reports

Tang ZS,Hao YH,Zhang EJ,Xu CL,Zhou Y,Zheng X,Yang DL

Published figure using CD28 monoclonal antibody (Product # 45-0289-42) in Flow Cytometry

Mon Aug 01 00:00:00 EDT 2016

IL12-mediated sensitizing of T-cell receptor-dependent and -independent tumor cell killing.

Oncoimmunology

Braun M,Ress ML,Yoo YE,Scholz CJ,Eyrich M,Schlegel PG,Wölfl M

45-0289 was used in Flow cytometry/Cell sorting to investigate how IL12, and other inflammatory cytokines, shape effector functions of human T-cells.

Fri Jul 01 00:00:00 EDT 2016

High numbers of differentiated effector CD4 T cells are found in patients with cancer and correlate with clinical response after neoadjuvant therapy of breast cancer.

Cancer research

Péguillet I,Milder M,Louis D,Vincent-Salomon A,Dorval T,Piperno-Neumann S,Scholl SM,Lantz O

45-0289 was used in Flow cytometry/Cell sorting to characterise a population of human differentiated effector CD4(+) T cells that is defined by low levels of the interleukin (IL)-2 and IL-7 receptors, showing that this population expands in various types of cancer and displays cytotoxic features, suggesting that they are involved in human antitumour response.

Tue Apr 15 00:00:00 EDT 2014

Aging and cytomegalovirus infection differentially and jointly affect distinct circulating T cell subsets in humans.

Journal of immunology (Baltimore, Md. : 1950)

Wertheimer AM,Bennett MS,Park B,Uhrlaub JL,Martinez C,Pulko V,Currier NL,Nikolich-Žugich D,Kaye J,Nikolich-Žugich J

Published figure using CD28 monoclonal antibody (Product # 45-0289-42) in Flow Cytometry

Sat Mar 01 00:00:00 EST 2014

Detailed characterization of tumor infiltrating lymphocytes in two distinct human solid malignancies show phenotypic similarities.

Journal for immunotherapy of cancer

Kovacsovics-Bankowski M,Chisholm L,Vercellini J,Tucker CG,Montler R,Haley D,Newell P,Ma J,Tseng P,Wolf R,Vetto JT,Hammill C,Hansen P,Weinberg AD

Published figure using CD28 monoclonal antibody (Product # 45-0289-42) in Flow Cytometry

Mon Dec 01 00:00:00 EST 2014

Small molecule inhibitor of antigen binding and presentation by HLA-DR2b as a therapeutic strategy for the treatment of multiple sclerosis.

Journal of immunology (Baltimore, Md. : 1950)

Ji N,Somanaboeina A,Dixit A,Kawamura K,Hayward NJ,Self C,Olson GL,Forsthuber TG

45-0289 was used in Flow cytometry/Cell sorting to investigate how blocking the HLA-DR2b allele with small molecule inhibitors may offer a promising therapeutic strategy for the treatment of multiple sclerosis.

Fri Nov 15 00:00:00 EST 2013

Expression of the memory marker CD45RO on helper T cells in macaques.

PloS one

Valentine M,Song K,Maresh GA,Mack H,Huaman MC,Polacino P,Ho O,Cristillo A,Kyung Chung H,Hu SL,Pincus SH

45-0289 was used in Flow cytometry/Cell sorting to identify and eliminate subsets of T cells known to house a latent reservoir of HIV.

Tue Apr 08 00:00:00 EDT 2014

Expression of the memory marker CD45RO on helper T cells in macaques.

PloS one

Valentine M,Song K,Maresh GA,Mack H,Huaman MC,Polacino P,Ho O,Cristillo A,Kyung Chung H,Hu SL,Pincus SH

45-0289 was used in Flow cytometry/Cell sorting to identify and eliminate subsets of T cells known to house a latent reservoir of HIV.

Tue Apr 08 00:00:00 EDT 2014

A novel method for autophagy detection in primary cells: impaired levels of macroautophagy in immunosenescent T cells.

Autophagy

Phadwal K,Alegre-Abarrategui J,Watson AS,Pike L,Anbalagan S,Hammond EM,Wade-Martins R,McMichael A,Klenerman P,Simon AK

Published figure using CD28 monoclonal antibody (Product # 45-0289-42) in Flow Cytometry

Sun Apr 01 00:00:00 EDT 2012

Vaccination with live attenuated simian immunodeficiency virus causes dynamic changes in intestinal CD4+CCR5+ T cells.

Retrovirology

Li B,Berry N,Ham C,Ferguson D,Smith D,Hall J,Page M,Quartey-Papafio R,Elsley W,Robinson M,Almond N,Stebbings R

45-0289 was used in Flow cytometry/Cell sorting to investigate whether target cell depletion contributes to the protection of infection with wild-type simian immunodeficiency virus.

Thu Feb 03 00:00:00 EST 2011

Bioinformatics

Protein Aliases: CD28; CD28 antigen; MGC138290; sCD28; soluble CD28; T-cell-specific surface glycoprotein CD28; T44; TP44

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Gene Aliases: CD28; Tp44

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UniProt ID: (Human) P10747

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Entrez Gene ID: (Human) 940

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Function(s)

protease binding SH3/SH2 adaptor activity protein binding coreceptor activity protein kinase binding identical protein binding phosphatidylinositol-4,5-bisphosphate 3-kinase activity immunoglobulin receptor superfamily

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

CD28 Monoclonal Antibody (CD28.2), PerCP-Cyanine5.5, eBioscience™

CD28 Antibody (45-0289-42) in Flow

Product Details

45-0289-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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