Invitrogen

CD28 Monoclonal Antibody (CD28.2), Biotin, eBioscience™

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Published Figures (30)

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CD28 Antibody (13-0289-82) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD28 Biotin followed by detection with Streptavidin PE (Product # 12-4317-87). Cells in the lymphocyte gate were used for analysis.

CD28 Antibody in T-Cell Activation (TCA)

CD28 Antibody in Functional Assay (Functional)

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Product Details

13-0289-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

1 µg/test

View 21 publications 21 publications

Functional Assay (Functional)

View 4 publications 4 publications

T-Cell Activation (TCA)

View 6 publications 6 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse, Rhesus monkey

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Biotin, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

CD28.2

Conjugate

Biotin Biotin Biotin

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_466415

Product Specific Information

Description: The CD28.2 monoclonal antibody reacts with the human CD28 molecule, a 44 kDa homodimer expressed by thymocytes, mature T cells and plasma cells. CD28 is a ligand for CD80 (B7-1) and CD86 (B7-2) and is a potent co-stimulator of T cells. Signaling through CD28 augments IL-2 and IL-2 receptor expression as well as cytotoxicity of CD3-activated T cells.

Applications Reported: The CD28.2 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The CD28.2 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD28 antigen is a 44 kDa disulfide linked homodimeric T cell specific surface glycoprotein. CD28 is a cell adhesion molecule of the immunoglobulin superfamily which is constitutively expressed on most peripheral blood T lymphocytes. Moreover, CD28 is the critical T cell costimulatory receptor that provides the cell the important second activation signal by binding CD80 and CD86 which are expressed by antigen presenting cells. In addition to its co-stimulation role, CD28 functions by preventing T cells from entering an anergic-hyporesponsive state or from undergoing premature apoptotic cell death. In murine peripheral lymphoid organs and in the blood, all CD4+ and CD8+ T cells express CD28. In the thymus, CD28 expression is highest on immature CD3-, CD8+ and CD4+8+ cells, and on CD4-8- cells that express alpha/beta and gamma/delta TCR. The level of CD28 on mature CD4+ and CD8+ alpha/beta TCR+ thymocytes is two- to fourfold lower than on the immature cells. Diseases associated with CD28 dysfunction include mycosis fungiodes and Sezary's Disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

CHI3L1 enhances melanoma lung metastasis via regulation of T cell co-stimulators and CTLA-4/B7 axis.

Frontiers in immunology

Ma B,Kamle S,Akosman B,Khan H,Lee CM,Lee CG,Elias JA

Published figure using CD28 monoclonal antibody (Product # 13-0289-82) in Flow Cytometry

Tue Jan 10 00:00:00 EST 2023

Atypical sideways recognition of CD1a by autoreactive γδ T cell receptors.

Nature communications

Wegrecki M,Ocampo TA,Gunasinghe SD,von Borstel A,Tin SY,Reijneveld JF,Cao TP,Gully BS,Le Nours J,Moody DB,Van Rhijn I,Rossjohn J

13-0289-82 was used in Immunocytochemistry-immunoflourescence to show that CD1a is a ligand for Vδ1+ γδ T cells.

Tue Jul 05 00:00:00 EDT 2022

Longitudinal profiling of respiratory and systemic immune responses reveals myeloid cell-driven lung inflammation in severe COVID-19.

Immunity

Szabo PA,Dogra P,Gray JI,Wells SB,Connors TJ,Weisberg SP,Krupska I,Matsumoto R,Poon MML,Idzikowski E,Morris SE,Pasin C,Yates AJ,Ku A,Chait M,Davis-Porada J,Guo XV,Zhou J,Steinle M,Mackay S,Saqi A,Baldwin MR,Sims PA,Farber DL

Published figure using CD28 monoclonal antibody (Product # 13-0289-82) in Flow Cytometry

Tue Apr 13 00:00:00 EDT 2021

Effects of the Antidiabetic Drugs Evogliptin and Sitagliptin on the Immune Function of CD26/DPP4 in Th1 Cells.

Biomolecules & therapeutics

Yoon H,Sung JH,Song MJ

Published figure using CD28 monoclonal antibody (Product # 13-0289-82) in Flow Cytometry

Mon Mar 01 00:00:00 EST 2021

CD19 Chimeric Antigen Receptor-Exosome Targets CD19 Positive B-lineage Acute Lymphocytic Leukemia and Induces Cytotoxicity.

Cancers

Haque S,Vaiselbuh SR

Published figure using CD28 monoclonal antibody (Product # 13-0289-82) in Flow Cytometry

Fri Mar 19 00:00:00 EDT 2021

Differences in Maturation Status and Immune Phenotypes of Circulating Helios⁺ and Helios^- Tregs and Their Disrupted Correlations With Monocyte Subsets in Autoantibody-Positive T1D Individuals.

Frontiers in immunology

Zhang Y,Zhang J,Shi Y,Shen M,Lv H,Chen S,Feng Y,Chen H,Xu X,Yang T,Xu K

Published figure using CD28 monoclonal antibody (Product # 13-0289-82) in Flow Cytometry

Wed Sep 15 00:00:00 EDT 2021

Metformin Enhances Autophagy and Normalizes Mitochondrial Function to Alleviate Aging-Associated Inflammation.

Cell metabolism

Bharath LP,Agrawal M,McCambridge G,Nicholas DA,Hasturk H,Liu J,Jiang K,Liu R,Guo Z,Deeney J,Apovian CM,Snyder-Cappione J,Hawk GS,Fleeman RM,Pihl RMF,Thompson K,Belkina AC,Cui L,Proctor EA,Kern PA,Nikolajczyk BS

Published figure using CD28 monoclonal antibody (Product # 13-0289-82) in T-Cell Activation

Tue Jul 07 00:00:00 EDT 2020

SIRT1 is downregulated by autophagy in senescence and ageing.

Nature cell biology

Xu C,Wang L,Fozouni P,Evjen G,Chandra V,Jiang J,Lu C,Nicastri M,Bretz C,Winkler JD,Amaravadi R,Garcia BA,Adams PD,Ott M,Tong W,Johansen T,Dou Z,Berger SL

Published figure using CD28 monoclonal antibody (Product # 13-0289-82) in Flow Cytometry

Thu Oct 01 00:00:00 EDT 2020

JNK1 and ERK1/2 modulate lymphocyte homeostasis via BIM and DRP1 upon AICD induction.

Cell death and differentiation

Simula L,Corrado M,Accordi B,Di Rita A,Nazio F,Antonucci Y,Di Daniele A,Caicci F,Caruana I,Soriano ME,Pigazzi M,Locatelli F,Cecconi F,Campello S

Published figure using CD28 monoclonal antibody (Product # 13-0289-82) in T-Cell Activation

Thu Oct 01 00:00:00 EDT 2020

PD-1 and BTLA regulate T cell signaling differentially and only partially through SHP1 and SHP2.

The Journal of cell biology

Xu X,Hou B,Fulzele A,Masubuchi T,Zhao Y,Wu Z,Hu Y,Jiang Y,Ma Y,Wang H,Bennett EJ,Fu G,Hui E

Published figure using CD28 monoclonal antibody (Product # 13-0289-82) in Flow Cytometry

Mon Jun 01 00:00:00 EDT 2020

Expression of CD27 and CD28 on γδ T cells from the peripheral blood of patients with allergic rhinitis.

Experimental and therapeutic medicine

Wang Q,Sun Q,Chen Q,Li H,Liu D

Published figure using CD28 monoclonal antibody (Product # 13-0289-82) in Flow Cytometry

Tue Dec 01 00:00:00 EST 2020

Human Liver Memory CD8⁺ T Cells Use Autophagy for Tissue Residence.

Cell reports

Swadling L,Pallett LJ,Diniz MO,Baker JM,Amin OE,Stegmann KA,Burton AR,Schmidt NM,Jeffery-Smith A,Zakeri N,Suveizdyte K,Froghi F,Fusai G,Rosenberg WM,Davidson BR,Schurich A,Simon AK,Maini MK

Published figure using CD28 monoclonal antibody (Product # 13-0289-82) in T-Cell Activation

Tue Jan 21 00:00:00 EST 2020

Selective Killing of Activated T Cells by 5-Aminolevulinic Acid Mediated Photodynamic Effect: Potential Improvement of Extracorporeal Photopheresis.

Cancers

Darvekar S,Juzenas P,Oksvold M,Kleinauskas A,Holien T,Christensen E,Stokke T,Sioud M,Peng Q

Published figure using CD28 monoclonal antibody (Product # 13-0289-82) in Flow Cytometry

Thu Feb 06 00:00:00 EST 2020

RAB11FIP5-Deficient Mice Exhibit Cytokine-Related Transcriptomic Signatures.

ImmunoHorizons

Li D,Bradley T,Cain DW,Pedroza-Pacheco I,Aggelakopoulou M,Parks R,Barr M,Xia SM,Scearce R,Bowman C,Stevens G,Newman A,Hora B,Chen Y,Riebe K,Wang Y,Sempowski G,Saunders KO,Borrow P,Haynes BF

Published figure using CD28 monoclonal antibody (Product # 13-0289-82) in Functional assay

Tue Nov 10 00:00:00 EST 2020

Cytotoxic T cells swarm by homotypic chemokine signalling.

eLife

Galeano Niño JL,Pageon SV,Tay SS,Colakoglu F,Kempe D,Hywood J,Mazalo JK,Cremasco J,Govendir MA,Dagley LF,Hsu K,Rizzetto S,Zieba J,Rice G,Prior V,O'Neill GM,Williams RJ,Nisbet DR,Kramer B,Webb AI,Luciani F,Read MN,Biro M

13-0289 was used in Flow cytometry/Cell sorting to reveal independent emergent behaviour in Cytotoxic T lymphocytes populations attacking tumour masses.

Tue Oct 13 00:00:00 EDT 2020

Flotillins promote T cell receptor sorting through a fast Rab5-Rab11 endocytic recycling axis.

Nature communications

Redpath GMI,Ecker M,Kapoor-Kaushik N,Vartoukian H,Carnell M,Kempe D,Biro M,Ariotti N,Rossy J

Published figure using CD28 monoclonal antibody (Product # 13-0289-82) in T-Cell Activation

Thu Sep 26 00:00:00 EDT 2019

Metabolic reprogramming of human CD8⁺ memory T cells through loss of SIRT1.

The Journal of experimental medicine

Jeng MY,Hull PA,Fei M,Kwon HS,Tsou CL,Kasler H,Ng CP,Gordon DE,Johnson J,Krogan N,Verdin E,Ott M

Published figure using CD28 monoclonal antibody (Product # 13-0289-82) in Flow Cytometry

Tue Jan 02 00:00:00 EST 2018

A Multistage Subunit Vaccine Effectively Protects Mice Against Primary Progressive Tuberculosis, Latency and Reactivation.

EBioMedicine

Ma J,Teng X,Wang X,Fan X,Wu Y,Tian M,Zhou Z,Li L

13-0289-82 was used in Flow Cytometry to conclude that CMFO-DMT represents a promising candidate to prevent the occurrence of adult TB through both prophylactic and therapeutic methods, and warrants assessment in preclinical and clinical trials.

Tue Aug 01 00:00:00 EDT 2017

Functional characterization of T-cells from palatine tonsils in patients with chronic tonsillitis.

PloS one

Geißler K,Markwart R,Requardt RP,Weigel C,Schubert K,Scherag A,Rubio I,Guntinas-Lichius O

13-0289 was used in Flow cytometry/Cell sorting to analyse and characterise the response of tonsillar T-cells from chronic tonsillitis to antigenic stimulation.

Mon Oct 16 00:00:00 EDT 2017

The Distribution of Human Stem Cell-like Memory T Cell in Lung Cancer.

Journal of immunotherapy (Hagerstown, Md. : 1997)

Hong H,Gu Y,Sheng SY,Lu CG,Zou JY,Wu CY

Published figure using CD28 monoclonal antibody (Product # 13-0289-82) in Flow Cytometry

Thu Jan 04 00:00:00 EST 2018

CD28 family of receptors on T cells in chronic HBV infection: Expression characteristics, clinical significance and correlations with PD-1 blockade.

Molecular medicine reports

Tang ZS,Hao YH,Zhang EJ,Xu CL,Zhou Y,Zheng X,Yang DL

Published figure using CD28 monoclonal antibody (Product # 13-0289-82) in Flow Cytometry

Mon Aug 01 00:00:00 EDT 2016

Aging and cytomegalovirus infection differentially and jointly affect distinct circulating T cell subsets in humans.

Journal of immunology (Baltimore, Md. : 1950)

Wertheimer AM,Bennett MS,Park B,Uhrlaub JL,Martinez C,Pulko V,Currier NL,Nikolich-Žugich D,Kaye J,Nikolich-Žugich J

13-0289 was used in Flow cytometry/Cell sorting to demonstrate that ageing and cytomegalovirus infection exert both distinct and joint influence upon blood T cell homeostasis in humans.

Sat Mar 01 00:00:00 EST 2014

N-glycosylation of asparagine 8 regulates surface expression of major histocompatibility complex class I chain-related protein A (MICA) alleles dependent on threonine 24.

The Journal of biological chemistry

Mellergaard M,Skovbakke SL,Schneider CL,Lauridsen F,Andresen L,Jensen H,Skov S

Published figure using CD28 monoclonal antibody (Product # 13-0289-82) in T-Cell Activation

Fri Jul 18 00:00:00 EDT 2014

Detailed characterization of tumor infiltrating lymphocytes in two distinct human solid malignancies show phenotypic similarities.

Journal for immunotherapy of cancer

Kovacsovics-Bankowski M,Chisholm L,Vercellini J,Tucker CG,Montler R,Haley D,Newell P,Ma J,Tseng P,Wolf R,Vetto JT,Hammill C,Hansen P,Weinberg AD

Published figure using CD28 monoclonal antibody (Product # 13-0289-82) in Flow Cytometry

Mon Dec 01 00:00:00 EST 2014

The autoimmune-predisposing variant of lymphoid tyrosine phosphatase favors T helper 1 responses.

Human immunology

Vang T,Landskron J,Viken MK,Oberprieler N,Torgersen KM,Mustelin T,Tasken K,Tautz L,Rickert RC,Lie BA

13-0289 was used in Flow cytometry/Cell sorting to provide a molecular and cellular explanation for the autoimmune phenotype associated with LYP*W620.

Wed May 01 00:00:00 EDT 2013

TCR activation kinetics and feedback regulation in primary human T cells.

Cell communication and signaling : CCS

Poltorak M,Arndt B,Kowtharapu BS,Reddycherla AV,Witte V,Lindquist JA,Schraven B,Simeoni L

13-0289 was used in Functional assays to analyse the signaling dynamics correlating with either unresponsiveness or proliferation induced upon TCR/CD28 ligation in primary human T cells, suggesting that alternative feedback regulation occurs during these conditions.

Mon Jan 14 00:00:00 EST 2013

A novel method for autophagy detection in primary cells: impaired levels of macroautophagy in immunosenescent T cells.

Autophagy

Phadwal K,Alegre-Abarrategui J,Watson AS,Pike L,Anbalagan S,Hammond EM,Wade-Martins R,McMichael A,Klenerman P,Simon AK

Published figure using CD28 monoclonal antibody (Product # 13-0289-82) in Flow Cytometry

Sun Apr 01 00:00:00 EDT 2012

T cell-signaling network analysis reveals distinct differences between CD28 and CD2 costimulation responses in various subsets and in the MAPK pathway between resting and activated regulatory T cells.

Journal of immunology (Baltimore, Md. : 1950)

Kalland ME,Oberprieler NG,Vang T,Taskén K,Torgersen KM

13-0289 was used in Binding experiments to reveal distinct differences between CD28 and CD2 costimulation responses in various subsets and in the MAPK pathway between resting and activated regulatory T cells.

Tue Nov 15 00:00:00 EST 2011

Cytokine requirements for the differentiation and expansion of IL-17A- and IL-22-producing human Vgamma2Vdelta2 T cells.

Journal of immunology (Baltimore, Md. : 1950)

Ness-Schwickerath KJ,Jin C,Morita CT

13-0289 was used in Flow cytometry/Cell sorting to determine if primate and murine gammadelta T cells play similar roles in being major sources of IL-17A in antimicrobial and autoimmune responses.

Tue Jun 15 00:00:00 EDT 2010

Cytokine requirements for the differentiation and expansion of IL-17A- and IL-22-producing human Vgamma2Vdelta2 T cells.

Journal of immunology (Baltimore, Md. : 1950)

Ness-Schwickerath KJ,Jin C,Morita CT

13-0289 was used in Flow cytometry/Cell sorting to determine if primate and murine gammadelta T cells play similar roles in being major sources of IL-17A in antimicrobial and autoimmune responses.

Tue Jun 15 00:00:00 EDT 2010

Dramatic increase in naive T cell turnover is linked to loss of naive T cells from old primates.

Proceedings of the National Academy of Sciences of the United States of America

Cicin-Sain L,Messaoudi I,Park B,Currier N,Planer S,Fischer M,Tackitt S,Nikolich-Zugich D,Legasse A,Axthelm MK,Picker LJ,Mori M,Nikolich-Zugich J

13-0289 was used in Flow cytometry/Cell sorting to examine the role of homeostatic cycling and proliferative expansion in different T cell subsets of ageing rhesus macaques.

Tue Dec 11 00:00:00 EST 2007

Phosphorylation of Bcl10 negatively regulates T-cell receptor-mediated NF-kappaB activation.

Molecular and cellular biology

Zeng H,Di L,Fu G,Chen Y,Gao X,Xu L,Lin X,Wen R

Published figure using CD28 monoclonal antibody (Product # 13-0289-82) in T-Cell Activation

Sun Jul 01 00:00:00 EDT 2007

Bioinformatics

Protein Aliases: CD28; CD28 antigen; MGC138290; sCD28; soluble CD28; T-cell-specific surface glycoprotein CD28; T44; TP44

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Gene Aliases: CD28; Tp44

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UniProt ID: (Human) P10747

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Entrez Gene ID: (Human) 940

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Function(s)

protease binding SH3/SH2 adaptor activity protein binding coreceptor activity protein kinase binding identical protein binding phosphatidylinositol-4,5-bisphosphate 3-kinase activity immunoglobulin receptor superfamily

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD28 Monoclonal Antibody (CD28.2), Biotin, eBioscience™

CD28 Antibody (13-0289-82) in Flow

Product Details

13-0289-82

Flow Cytometry (Flow)

Functional Assay (Functional)

T-Cell Activation (TCA)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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