Invitrogen

CD61 (Integrin beta 3) Monoclonal Antibody (2C9.G3), FITC, eBioscience™

CD61 (Integrin beta 3) Antibody in Flow Cytometry (Flow)

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CD61 (Integrin beta 3) Antibody (11-0611-82) in Flow

Staining of C57BL/6 bone marrow cells with 0.5 µg of FITC Armenian Hamster IgG Isotype Control (Product # 11-4888-81) (open histogram) or 0.5 µg of Anti-Mouse/Rat CD61 (Integrin beta 3) FITC (filled histogram). Cells in the lymphoid (left) or myeloid (right) gates were used for analysis.

CD61 (Integrin beta 3) Antibody in Immunocytochemistry (ICC/IF)

CD61 (Integrin beta 3) Antibody in Immunohistochemistry (IHC)

CD61 (Integrin beta 3) Antibody in Western Blot (WB)

CD61 (Integrin beta 3) Antibody in Neutralization (Neu)

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Product Details

11-0611-82

Applications

Tested Dilution

Publications

Western Blot (WB)

View 2 publications 2 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

View 4 publications 4 publications

Flow Cytometry (Flow)

1 µg/test

View 11 publications 11 publications

Neutralization (Neu)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse, Rat

Published species

Mouse

Host/Isotype

Armenian hamster / IgG

Recommended Isotype Control

Armenian Hamster IgG Isotype Control (eBio299Arm), FITC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

2C9.G3

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_465106

Product Specific Information

Description: The 2C9.G3 (HMb3-1) monoclonal antibody reacts with mouse and rat CD61, also known as the integrin beta3. CD61 is expressed by activated T cells, granulocytes, and platelet. CD61 associates non-covalently with two integrin alpha subunits; alphaV (CD51) to form Vitronectin Receptor and alphaIib (CD41) to form gpIIb/IIIa. These heterodimeric complexes are responsible for adhesion to extracellular matrix components including fibrinogen, fibronectin, fibronectin, vitronectin, thrombospondin and von Willebrand factor.

Applications Reported: The 2C9.G3 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The 2C9.G3 antibody has been tested by flow cytometric analysis of mouse splenocytes and bone marrow cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD61 (GPIIIa, ITGB3) is a glycoprotein found on megakaryocytes, platelets and their precursors. CD61 antigen plays a role in platelet aggregation and also as a receptor for fibrinogen, fibronectin, von Willebrand factor and vitronectrin. CD61 is a 105 kDa glycoprotein that associates with either the alpha 11b integrin (CD41) or the alpha V integrin (CD51) at the cell surface. CD61 is expressed on platelets and megakaryocytes in association with CD41, and on endothelial cells, monocytes, platelets and osteoclasts in association with CD51. CD61 is a receptor for fibrinogen, fibronectin, Von Willebrand Factor, vitronectin and thrombospondin. The CD61 protein product is composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. CD61 is found along with the alpha IIb chain in platelets. Like other integrins, CD61 is known to participate in cell adhesion as well as cell-surface mediated signaling. Diseases associated with CD61 dysfunction include Glanzmann Thrombasthenia and Platelet type-16 Bleeding Disorder.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

A Small Molecule Inhibitor, OGP46, Is Effective against Imatinib-Resistant BCR-ABL Mutations via the BCR-ABL/JAK-STAT Pathway.

Molecular therapy oncolytics

Wei L,Yang Y,Gupta P,Wang A,Zhao M,Zhao Y,Qu M,Ke Y,Liu Y,Liu HM,Xu X,Sun Y,Chen ZS,Hu Z

Published figure using CD61 (Integrin beta 3) monoclonal antibody (Product # 11-0611-82) in Immunohistochemistry

Fri Sep 25 00:00:00 EDT 2020

Regulatory T Cells Control the Switch From in situ to Invasive Breast Cancer.

Frontiers in immunology

Martinez LM,Robila V,Clark NM,Du W,Idowu MO,Rutkowski MR,Bos PD

11-0611-82 was used in Flow cytometry/Cell sorting to investigate Treg cell function in the progression from non-invasive to invasive breast cancer lesions.

Tue Sep 29 00:00:00 EDT 2020

Establishment of regulatory elements during erythro-megakaryopoiesis identifies hematopoietic lineage-commitment points.

Epigenetics & chromatin

Heuston EF,Keller CA,Lichtenberg J,Giardine B,Anderson SM,Hardison RC,Bodine DM

11-0611 was used in Flow cytometry/Cell sorting to demonstrate that megakaryopoietic profiles are established early in haematopoiesis and are present in the majority of the haematopoietic progenitor population.

Mon May 28 00:00:00 EDT 2018

Hoxa9 and Meis1 Cooperatively Induce Addiction to Syk Signaling by Suppressing miR-146a in Acute Myeloid Leukemia.

Cancer cell

Mohr S,Doebele C,Comoglio F,Berg T,Beck J,Bohnenberger H,Alexe G,Corso J,Ströbel P,Wachter A,Beissbarth T,Schnütgen F,Cremer A,Haetscher N,Göllner S,Rouhi A,Palmqvist L,Rieger MA,Schroeder T,Bönig H,Müller-Tidow C,Kuchenbauer F,Schütz E,Green AR,Urlaub H,Stegmaier K,Humphries RK,Serve H,Oellerich T

Published figure using CD61 (Integrin beta 3) monoclonal antibody (Product # 11-0611-82) in Flow Cytometry

Mon Apr 10 00:00:00 EDT 2017

The fibronectin synergy site re-enforces cell adhesion and mediates a crosstalk between integrin classes.

eLife

Benito-Jardón M,Klapproth S,Gimeno-LLuch I,Petzold T,Bharadwaj M,Müller DJ,Zuchtriegel G,Reichel CA,Costell M

Published figure using CD61 (Integrin beta 3) monoclonal antibody (Product # 11-0611-82) in Immunofluorescence

Mon Jan 16 00:00:00 EST 2017

The fibronectin synergy site re-enforces cell adhesion and mediates a crosstalk between integrin classes.

eLife

Benito-Jardón M,Klapproth S,Gimeno-LLuch I,Petzold T,Bharadwaj M,Müller DJ,Zuchtriegel G,Reichel CA,Costell M

Published figure using CD61 (Integrin beta 3) monoclonal antibody (Product # 11-0611-82) in Immunofluorescence

Mon Jan 16 00:00:00 EST 2017

WISP1-αvβ3 integrin signaling positively regulates TLR-triggered inflammation response in sepsis induced lung injury.

Scientific reports

Chen Z,Ding X,Jin S,Pitt B,Zhang L,Billiar T,Li Q

Published figure using CD61 (Integrin beta 3) monoclonal antibody (Product # 11-0611-82) in Neutralization

Tue Jun 28 00:00:00 EDT 2016

WISP1-αvβ3 integrin signaling positively regulates TLR-triggered inflammation response in sepsis induced lung injury.

Scientific reports

Chen Z,Ding X,Jin S,Pitt B,Zhang L,Billiar T,Li Q

Published figure using CD61 (Integrin beta 3) monoclonal antibody (Product # 11-0611-82) in Neutralization

Tue Jun 28 00:00:00 EDT 2016

H2S protects against fatal myelosuppression by promoting the generation of megakaryocytes/platelets.

Journal of hematology & oncology

Liu HD,Zhang AJ,Xu JJ,Chen Y,Zhu YC

Published figure using CD61 (Integrin beta 3) monoclonal antibody (Product # 11-0611-82) in Immunofluorescence

Wed Feb 24 00:00:00 EST 2016

Reprogramming mouse fibroblasts into engraftable myeloerythroid and lymphoid progenitors.

Nature communications

Cheng H,Ang HY,A El Farran C,Li P,Fang HT,Liu TM,Kong SL,Chin ML,Ling WY,Lim EK,Li H,Huber T,Loh KM,Loh YH,Lim B

11-0611 was used in Flow cytometry/Cell sorting to demonstrate how it is possible to reprogram mouse embryonic fibroblasts into engraftable blood progenitors.

Mon Nov 21 00:00:00 EST 2016

A crucial role for β2 integrins in podosome formation, dynamics and Toll-like-receptor-signaled disassembly in dendritic cells.

Journal of cell science

Gawden-Bone C,West MA,Morrison VL,Edgar AJ,McMillan SJ,Dill BD,Trost M,Prescott A,Fagerholm SC,Watts C

11-0611 was used in Immunofluorescence to assess the contribution of integrins to the function and maintenance of podosomes.

Wed Oct 01 00:00:00 EDT 2014

Integrin-modulating therapy prevents fibrosis and autoimmunity in mouse models of scleroderma.

Nature

Gerber EE,Gallo EM,Fontana SC,Davis EC,Wigley FM,Huso DL,Dietz HC

Published figure using CD61 (Integrin beta 3) monoclonal antibody (Product # 11-0611-82) in Flow Cytometry

Thu Nov 07 00:00:00 EST 2013

Evidence for a multipotent mammary progenitor with pregnancy-specific activity.

Breast cancer research : BCR

Kaanta AS,Virtanen C,Selfors LM,Brugge JS,Neel BG

Published figure using CD61 (Integrin beta 3) monoclonal antibody (Product # 11-0611-82) in Immunofluorescence

Mon Dec 08 00:00:00 EST 2014

β1 integrins with individually disrupted cytoplasmic NPxY motifs are embryonic lethal but partially active in the epidermis.

The Journal of investigative dermatology

Meves A,Stremmel C,Böttcher RT,Fässler R

Published figure using CD61 (Integrin beta 3) monoclonal antibody (Product # 11-0611-82) in Flow Cytometry

Sun Dec 01 00:00:00 EST 2013

Differential regulation of osteoclastogenesis by Notch2/Delta-like 1 and Notch1/Jagged1 axes.

Arthritis research & therapy

Sekine C,Koyanagi A,Koyama N,Hozumi K,Chiba S,Yagita H

11-0611 was used in Flow cytometry/Cell sorting to determine the role of individual Notch receptors and ligands in osteoclastogenesis..

Mon Mar 05 00:00:00 EST 2012

Differentiation generates paracrine cell pairs that maintain basaloid mouse mammary tumors: proof of concept.

PloS one

Kim S,Goel S,Alexander CM

Published figure using CD61 (Integrin beta 3) monoclonal antibody (Product # 11-0611-82) in Flow Cytometry

Tue Apr 26 00:00:00 EDT 2011

Thrombocytopenia in mice lacking the carboxy-terminal regulatory domain of the Ets transcription factor Fli1.

Molecular and cellular biology

Moussa O,LaRue AC,Abangan RS,Williams CR,Zhang XK,Masuya M,Gong YZ,Spyropoulos DD,Ogawa M,Gilkeson G,Watson DK

11-0611 was used in Flow cytometry/Cell sorting to demonstrate a haemostatic defect associated with the loss of a specific functional domain of the transcription factor Fli1 and suggest previously unknown in vivo roles in megakaryocytic cell differentiation.

Mon Nov 01 00:00:00 EDT 2010

Plasminogen activator inhibitor-1 regulates integrin alphavbeta3 expression and autocrine transforming growth factor beta signaling.

The Journal of biological chemistry

Pedroja BS,Kang LE,Imas AO,Carmeliet P,Bernstein AM

Published figure using CD61 (Integrin beta 3) monoclonal antibody (Product # 11-0611-82) in Immunofluorescence

Fri Jul 31 00:00:00 EDT 2009

Plasminogen activator inhibitor-1 regulates integrin alphavbeta3 expression and autocrine transforming growth factor beta signaling.

The Journal of biological chemistry

Pedroja BS,Kang LE,Imas AO,Carmeliet P,Bernstein AM

Published figure using CD61 (Integrin beta 3) monoclonal antibody (Product # 11-0611-82) in Immunofluorescence

Fri Jul 31 00:00:00 EDT 2009

Constitutively active aryl hydrocarbon receptor expressed specifically in T-lineage cells causes thymus involution and suppresses the immunization-induced increase in splenocytes.

Journal of immunology (Baltimore, Md. : 1950)

Nohara K,Pan X,Tsukumo S,Hida A,Ito T,Nagai H,Inouye K,Motohashi H,Yamamoto M,Fujii-Kuriyama Y,Tohyama C

Tue Mar 01 00:00:00 EST 2005

Bioinformatics

Protein Aliases: CD61; GPIIIa; integrin beta 3 (Cd61); Integrin beta-3; platelet gpIIIa; Platelet membrane glycoprotein IIIa

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Gene Aliases: CD61; GP3A; INGRB3; Itgb3

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UniProt ID: (Rat) Q8R2H2, (Mouse) O54890

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Entrez Gene ID: (Rat) 29302, (Mouse) 16416

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Function(s)

fibronectin binding protease binding protein disulfide isomerase activity integrin binding protein binding peptide binding identical protein binding vascular endothelial growth factor receptor 2 binding cell adhesion molecule binding extracellular matrix binding fibrinogen binding receptor activity integrin

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD61 (Integrin beta 3) Monoclonal Antibody (2C9.G3), FITC, eBioscience™

CD61 (Integrin beta 3) Antibody (11-0611-82) in Flow

Product Details

11-0611-82

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Neutralization (Neu)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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