Invitrogen

CD61 (Integrin beta 3) Monoclonal Antibody (2C9.G3), PE, eBioscience™

CD61 (Integrin beta 3) Antibody in Flow Cytometry (Flow)

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CD61 (Integrin beta 3) Antibody (12-0611-82) in Flow

Staining of mouse bone marrow cells with Armenian Hamster IgG Isotype Control FITC (Product # 11-4888-81) (left, open histogram) or Armenian Hamster IgG Isotype Control PE (Product # 12-4888-81) (left, open histogram) and Anti-Mouse/Rat CD61 (Integrin beta 3) FITC (Product # 11-0611-82) (left, filled histogram) and 0.25 µg of Anti-Mouse/Rat CD61 (Integrin beta 3) PE (right, filled histogram). Cells in the m... View More

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Product Details

12-0611-82

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

View 1 publication 1 publication

Flow Cytometry (Flow)

0.5 µg/test

View 17 publications 17 publications

Product Specifications

Species Reactivity

Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Armenian hamster / IgG

Recommended Isotype Control

Armenian Hamster IgG Isotype Control (eBio299Arm), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

2C9.G3

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_465718

Product Specific Information

Description: The 2C9.G3 (HMb3-1) monoclonal antibody reacts with mouse and rat CD61, also known as the integrin beta3. CD61 is expressed by activated T cells, granulocytes, and platelet. CD61 associates non-covalently with two integrin alpha subunits; alphaV (CD51) to form Vitronectin Receptor and alphaIib (CD41) to form gpIIb/IIIa. These heterodimeric complexes are responsible for adhesion to extracellular matrix components including fibrinogen, fibronectin, fibronectin, vitronectin, thrombospondin and von Willebrand factor.

Applications Reported: The 2C9.G3 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The 2C9.G3 antibody has been tested by flow cytometric analysis of mouse splenocytes and bone marrow cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD61 (GPIIIa, ITGB3) is a glycoprotein found on megakaryocytes, platelets and their precursors. CD61 antigen plays a role in platelet aggregation and also as a receptor for fibrinogen, fibronectin, von Willebrand factor and vitronectrin. CD61 is a 105 kDa glycoprotein that associates with either the alpha 11b integrin (CD41) or the alpha V integrin (CD51) at the cell surface. CD61 is expressed on platelets and megakaryocytes in association with CD41, and on endothelial cells, monocytes, platelets and osteoclasts in association with CD51. CD61 is a receptor for fibrinogen, fibronectin, Von Willebrand Factor, vitronectin and thrombospondin. The CD61 protein product is composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. CD61 is found along with the alpha IIb chain in platelets. Like other integrins, CD61 is known to participate in cell adhesion as well as cell-surface mediated signaling. Diseases associated with CD61 dysfunction include Glanzmann Thrombasthenia and Platelet type-16 Bleeding Disorder.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Human-Immune-System (HIS) humanized mouse model (DRAGA: HLA-A2.HLA-DR4.Rag1KO.IL-2RγcKO.NOD) for COVID-19.

Human vaccines & immunotherapeutics

Brumeanu TD,Vir P,Karim AF,Kar S,Benetiene D,Lok M,Greenhouse J,Putmon-Taylor T,Kitajewski C,Chung KK,Pratt KP,Casares SA

12-0611-82 was used in Immunohistochemistry (Paraffin) to report a Human Immune System (HIS)-humanized mouse model (DRAGA": HLA-A2.HLA-DR4.Rag1KO.IL-2 RγcKO.NOD) for COVID-19 research."

Wed Nov 30 00:00:00 EST 2022

A Human-Immune-System (HIS) humanized mouse model (DRAGA: HLA-A2. HLA-DR4. Rag1 KO.IL-2Rγc KO. NOD) for COVID-19.

bioRxiv : the preprint server for biology

Brumeanu TD,Vir P,Karim AF,Kar S,Benetiene D,Lok M,Greenhouse J,Putmon-Taylor T,Kitajewski C,Chung KK,Pratt KP,Casares SA

12-0611 was used in Immunohistochemistry-immunofluorescence to develop a novel pre-clinical mouse model to study COVID-19 immunopathology.

Fri Jan 29 00:00:00 EST 2021

C3G contributes to platelet activation and aggregation by regulating major signaling pathways.

Signal transduction and targeted therapy

Gutiérrez-Herrero S,Fernández-Infante C,Hernández-Cano L,Ortiz-Rivero S,Guijas C,Martín-Granado V,González-Porras JR,Balsinde J,Porras A,Guerrero C

12-0611 was used in Flow cytometry/Cell sorting to reveal the critical role of C3G in the main pathways leading to platelet activation and aggregation through the regulation of Rap1b.

Wed Apr 01 00:00:00 EDT 2020

β1 Integrin regulates convergent extension in mouse notogenesis, ensures notochord integrity and the morphogenesis of vertebrae and intervertebral discs.

Development (Cambridge, England)

Guo SS,Au TYK,Wynn S,Aszodi A,Chan D,Fässler R,Cheah KSE

12-0611-82 was used in Flow Cytometry to implicate pivotal roles of β1 integrin for the establishment of PCP and convergent extension of the developing notochord, its structural integrity and positioning.

Wed Nov 18 00:00:00 EST 2020

Comprehensive Cell Surface Antigen Analysis Identifies Transferrin Receptor Protein-1 (CD71) as a Negative Selection Marker for Human Neuronal Cells.

Stem cells (Dayton, Ohio)

Menon V,Thomas R,Elgueta C,Horl M,Osborn T,Hallett PJ,Bartos M,Isacson O,Pruszak J

Tue Oct 01 00:00:00 EDT 2019

Hoxa9 and Meis1 Cooperatively Induce Addiction to Syk Signaling by Suppressing miR-146a in Acute Myeloid Leukemia.

Cancer cell

Mohr S,Doebele C,Comoglio F,Berg T,Beck J,Bohnenberger H,Alexe G,Corso J,Ströbel P,Wachter A,Beissbarth T,Schnütgen F,Cremer A,Haetscher N,Göllner S,Rouhi A,Palmqvist L,Rieger MA,Schroeder T,Bönig H,Müller-Tidow C,Kuchenbauer F,Schütz E,Green AR,Urlaub H,Stegmaier K,Humphries RK,Serve H,Oellerich T

12-0611 was used in Flow cytometry/Cell sorting to study the effects of combined Hoxa9/Meis1 over-expression on intracellular signalling, and whether cells transformed by Hoxa9 and Meis1 become addicted to targetable signalling processes.

Mon Apr 10 00:00:00 EDT 2017

The fibronectin synergy site re-enforces cell adhesion and mediates a crosstalk between integrin classes.

eLife

Benito-Jardón M,Klapproth S,Gimeno-LLuch I,Petzold T,Bharadwaj M,Müller DJ,Zuchtriegel G,Reichel CA,Costell M

Published figure using CD61 (Integrin beta 3) monoclonal antibody (Product # 12-0611-82) in Flow Cytometry

Mon Jan 16 00:00:00 EST 2017

Mammary-Stem-Cell-Based Somatic Mouse Models Reveal Breast Cancer Drivers Causing Cell Fate Dysregulation.

Cell reports

Zhang Z,Christin JR,Wang C,Ge K,Oktay MH,Guo W

Published figure using CD61 (Integrin beta 3) monoclonal antibody (Product # 12-0611-82) in Flow Cytometry

Tue Sep 20 00:00:00 EDT 2016

H2S protects against fatal myelosuppression by promoting the generation of megakaryocytes/platelets.

Journal of hematology & oncology

Liu HD,Zhang AJ,Xu JJ,Chen Y,Zhu YC

Published figure using CD61 (Integrin beta 3) monoclonal antibody (Product # 12-0611-82) in Flow Cytometry

Wed Feb 24 00:00:00 EST 2016

Laminin 521 maintains differentiation potential of mouse and human satellite cell-derived myoblasts during long-term culture expansion.

Skeletal muscle

Penton CM,Badarinarayana V,Prisco J,Powers E,Pincus M,Allen RE,August PR

12-0611 was used in Flow cytometry/Cell sorting to demonstrate laminin 521 is a superior substrate for both short-term and long-term myogenic cell culture applications compared to other commonly utilized substrates.

Tue Dec 13 00:00:00 EST 2016

Acute depletion of endothelial β3-integrin transiently inhibits tumor growth and angiogenesis in mice.

Circulation research

Steri V,Ellison TS,Gontarczyk AM,Weilbaecher K,Schneider JG,Edwards D,Fruttiger M,Hodivala-Dilke KM,Robinson SD

12-0611 was used in Flow cytometry/Cell sorting to show that acute depletion of endothelial β3-integrin inhibits tumor growth and angiogenesis preventatively, but not in already established tumours.

Fri Jan 03 00:00:00 EST 2014

Co-targeting of convergent nucleotide biosynthetic pathways for leukemia eradication.

The Journal of experimental medicine

Nathanson DA,Armijo AL,Tom M,Li Z,Dimitrova E,Austin WR,Nomme J,Campbell DO,Ta L,Le TM,Lee JT,Darvish R,Gordin A,Wei L,Liao HI,Wilks M,Martin C,Sadeghi S,Murphy JM,Boulos N,Phelps ME,Faull KF,Herschman HR,Jung ME,Czernin J,Lavie A,Radu CG

12-0611 was used in Flow cytometry/Cell sorting to identify dCTP biosynthesis as a potential new therapeutic target for metabolic interventions in haematological malignancies.

Mon Mar 10 00:00:00 EDT 2014

Co-targeting of convergent nucleotide biosynthetic pathways for leukemia eradication.

The Journal of experimental medicine

12-0611 was used in Flow cytometry/Cell sorting to identify dCTP biosynthesis as a potential new therapeutic target for metabolic interventions in haematological malignancies.

Mon Mar 10 00:00:00 EDT 2014

Integrin-modulating therapy prevents fibrosis and autoimmunity in mouse models of scleroderma.

Nature

Gerber EE,Gallo EM,Fontana SC,Davis EC,Wigley FM,Huso DL,Dietz HC

Published figure using CD61 (Integrin beta 3) monoclonal antibody (Product # 12-0611-82) in Flow Cytometry

Thu Nov 07 00:00:00 EST 2013

β1 integrins with individually disrupted cytoplasmic NPxY motifs are embryonic lethal but partially active in the epidermis.

The Journal of investigative dermatology

Meves A,Stremmel C,Böttcher RT,Fässler R

12-0611 was used in Flow cytometry/Cell sorting to study the regulation of Β1 Integrin adhesion through talins and kindlins in keratinocytes and cells of the developing embryo.

Sun Dec 01 00:00:00 EST 2013

Role for E-cadherin as an inhibitory receptor on epidermal gammadelta T cells.

Journal of immunology (Baltimore, Md. : 1950)

Uchida Y,Kawai K,Ibusuki A,Kanekura T

12-0611 was used in Flow cytometry/Cell sorting to analyse functional differences between E-cadherin and α(E)β(7) integrin expressed on murine dendritic epidermal T cells.

Wed Jun 15 00:00:00 EDT 2011

Differentiation generates paracrine cell pairs that maintain basaloid mouse mammary tumors: proof of concept.

PloS one

Kim S,Goel S,Alexander CM

Published figure using CD61 (Integrin beta 3) monoclonal antibody (Product # 12-0611-82) in Flow Cytometry

Tue Apr 26 00:00:00 EDT 2011

Characterization of fibroblasts responsible for cartilage destruction in arthritis.

Cytometry. Part A : the journal of the International Society for Analytical Cytology

Treese C,Mittag A,Lange F,Tarnok A,Loesche A,Emmrich F,Lehmann J,Sack U

12-0611 was used in Flow cytometry/Cell sorting to show that LS48 is an excellent tool to gain a deeper understanding of synovial fibroblasts in rheumatoid arthritis.

Tue Apr 01 00:00:00 EDT 2008

The Foxc2 transcription factor regulates angiogenesis via induction of integrin beta3 expression.

The Journal of biological chemistry

Hayashi H,Sano H,Seo S,Kume T

12-0611 was used in Flow cytometry/Cell sorting to suggest that Foxc2 directly induces Itgb3 expression and regulates angiogenesis by Itgb3-mediated endothelial cell adhesion and migration.

Fri Aug 29 00:00:00 EDT 2008

Constitutively active aryl hydrocarbon receptor expressed specifically in T-lineage cells causes thymus involution and suppresses the immunization-induced increase in splenocytes.

Journal of immunology (Baltimore, Md. : 1950)

Nohara K,Pan X,Tsukumo S,Hida A,Ito T,Nagai H,Inouye K,Motohashi H,Yamamoto M,Fujii-Kuriyama Y,Tohyama C

Tue Mar 01 00:00:00 EST 2005

Bioinformatics

Protein Aliases: CD61; GPIIIa; integrin beta 3 (Cd61); Integrin beta-3; platelet gpIIIa; Platelet membrane glycoprotein IIIa

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Gene Aliases: CD61; GP3A; INGRB3; Itgb3

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UniProt ID: (Rat) Q8R2H2, (Mouse) O54890

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Entrez Gene ID: (Rat) 29302, (Mouse) 16416

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Function(s)

fibronectin binding protease binding protein disulfide isomerase activity integrin binding protein binding peptide binding identical protein binding vascular endothelial growth factor receptor 2 binding cell adhesion molecule binding extracellular matrix binding fibrinogen binding receptor activity integrin

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD61 (Integrin beta 3) Monoclonal Antibody (2C9.G3), PE, eBioscience™

CD61 (Integrin beta 3) Antibody (12-0611-82) in Flow

Product Details

12-0611-82

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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