Invitrogen

CD61 (Integrin beta 3) Monoclonal Antibody (2C9.G3), Biotin, eBioscience™

CD61 (Integrin beta 3) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 21

CD61 (Integrin beta 3) Antibody (13-0611-81) in Flow

Staining of C57BL/6 bone marrow cells with 0.06 µg of Golden Syrian Hamster IgG Isotype Control Biotin (Product # 13-4914-81) (open histogram) or 0.06 µg of Anti-Mouse/Rat CD61 (Integrin beta 3) Biotin (filled histogram) followed by Streptavidin PE (Product # 12-4317-87). Cells in the large scatter population were used for analysis.

CD61 (Integrin beta 3) Antibody in Western Blot (WB)

CD61 (Integrin beta 3) Antibody in Immunocytochemistry (ICC/IF)

CD61 (Integrin beta 3) Antibody in Immunohistochemistry (IHC)

CD61 (Integrin beta 3) Antibody in Neutralization (Neu)

Product Image Gallery

Product Details

13-0611-81

Applications

Tested Dilution

Publications

Western Blot (WB)

View 2 publications 2 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

View 3 publications 3 publications

Flow Cytometry (Flow)

0.125 µg/test

View 12 publications 12 publications

Neutralization (Neu)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Armenian hamster / IgG

Recommended Isotype Control

Armenian Hamster IgG Isotype Control (eBio299Arm), Biotin, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

2C9.G3

Conjugate

Biotin Biotin Biotin

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_466487

Product Specific Information

Description: The 2C9.G3 (HMb3-1) monoclonal antibody reacts with mouse and rat CD61, also known as the integrin beta3. CD61 is expressed by activated T cells, granulocytes, and platelet. CD61 associates non-covalently with two integrin alpha subunits; alphaV (CD51) to form Vitronectin Receptor and alphaIib (CD41) to form gpIIb/IIIa. These heterodimeric complexes are responsible for adhesion to extracellular matrix components including fibrinogen, fibronectin, fibronectin, vitronectin, thrombospondin and von Willebrand factor.

Applications Reported: The 2C9.G3 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The 2C9.G3 antibody has been tested by flow cytometric analysis of mouse splenocytes and bone marrow cells. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD61 (GPIIIa, ITGB3) is a glycoprotein found on megakaryocytes, platelets and their precursors. CD61 antigen plays a role in platelet aggregation and also as a receptor for fibrinogen, fibronectin, von Willebrand factor and vitronectrin. CD61 is a 105 kDa glycoprotein that associates with either the alpha 11b integrin (CD41) or the alpha V integrin (CD51) at the cell surface. CD61 is expressed on platelets and megakaryocytes in association with CD41, and on endothelial cells, monocytes, platelets and osteoclasts in association with CD51. CD61 is a receptor for fibrinogen, fibronectin, Von Willebrand Factor, vitronectin and thrombospondin. The CD61 protein product is composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. CD61 is found along with the alpha IIb chain in platelets. Like other integrins, CD61 is known to participate in cell adhesion as well as cell-surface mediated signaling. Diseases associated with CD61 dysfunction include Glanzmann Thrombasthenia and Platelet type-16 Bleeding Disorder.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

A Small Molecule Inhibitor, OGP46, Is Effective against Imatinib-Resistant BCR-ABL Mutations via the BCR-ABL/JAK-STAT Pathway.

Molecular therapy oncolytics

Wei L,Yang Y,Gupta P,Wang A,Zhao M,Zhao Y,Qu M,Ke Y,Liu Y,Liu HM,Xu X,Sun Y,Chen ZS,Hu Z

13-0611 was used in Flow cytometry/Cell sorting to highlight that OGP46 is active against not only native BCR-ABL but also 11 clinically relevant BCR-ABL mutations, including T315I mutation, which are resistant to imatinib.

Fri Sep 25 00:00:00 EDT 2020

A Small Molecule Inhibitor, OGP46, Is Effective against Imatinib-Resistant BCR-ABL Mutations via the BCR-ABL/JAK-STAT Pathway.

Molecular therapy oncolytics

Wei L,Yang Y,Gupta P,Wang A,Zhao M,Zhao Y,Qu M,Ke Y,Liu Y,Liu HM,Xu X,Sun Y,Chen ZS,Hu Z

Fri Sep 25 00:00:00 EDT 2020

Polychromic Reporter Mice Reveal Unappreciated Innate Lymphoid Cell Progenitor Heterogeneity and Elusive ILC3 Progenitors in Bone Marrow.

Immunity

Walker JA,Clark PA,Crisp A,Barlow JL,Szeto A,Ferreira ACF,Rana BMJ,Jolin HE,Rodriguez-Rodriguez N,Sivasubramaniam M,Pannell R,Cruickshank J,Daly M,Haim-Vilmovsky L,Teichmann SA,McKenzie ANJ

13-0611-81 was used in Flow Cytometry to identify a discrete ILC2-committed population and delineated transition states between early progenitors and a highly heterogeneous ILC1, ILC3, and NK precursor cell cluster.

Tue Jul 16 00:00:00 EDT 2019

Repurposing an anti-cancer agent for the treatment of hypertrophic heart disease.

The Journal of pathology

Dukinfield M,Maniati E,Reynolds LE,Aubdool A,Baliga RS,D'Amico G,Maiques O,Wang J,Bedi KC,Margulies KB,Sanz-Moreno V,Hobbs A,Hodivala-Dilke K

Published figure using CD61 (Integrin beta 3) monoclonal antibody (Product # 13-0611-81) in Immunohistochemistry

Sun Dec 01 00:00:00 EST 2019

Hoxa9 and Meis1 Cooperatively Induce Addiction to Syk Signaling by Suppressing miR-146a in Acute Myeloid Leukemia.

Cancer cell

Mohr S,Doebele C,Comoglio F,Berg T,Beck J,Bohnenberger H,Alexe G,Corso J,Ströbel P,Wachter A,Beissbarth T,Schnütgen F,Cremer A,Haetscher N,Göllner S,Rouhi A,Palmqvist L,Rieger MA,Schroeder T,Bönig H,Müller-Tidow C,Kuchenbauer F,Schütz E,Green AR,Urlaub H,Stegmaier K,Humphries RK,Serve H,Oellerich T

Published figure using CD61 (Integrin beta 3) monoclonal antibody (Product # 13-0611-81) in Flow Cytometry

Mon Apr 10 00:00:00 EDT 2017

The fibronectin synergy site re-enforces cell adhesion and mediates a crosstalk between integrin classes.

eLife

Benito-Jardón M,Klapproth S,Gimeno-LLuch I,Petzold T,Bharadwaj M,Müller DJ,Zuchtriegel G,Reichel CA,Costell M

Published figure using CD61 (Integrin beta 3) monoclonal antibody (Product # 13-0611-81) in Flow Cytometry

Mon Jan 16 00:00:00 EST 2017

The fibronectin synergy site re-enforces cell adhesion and mediates a crosstalk between integrin classes.

eLife

Benito-Jardón M,Klapproth S,Gimeno-LLuch I,Petzold T,Bharadwaj M,Müller DJ,Zuchtriegel G,Reichel CA,Costell M

Published figure using CD61 (Integrin beta 3) monoclonal antibody (Product # 13-0611-81) in Flow Cytometry

Mon Jan 16 00:00:00 EST 2017

Opposing roles of Nfkb2 gene products p100 and p52 in the regulation of breast cancer stem cells.

Breast cancer research and treatment

Yeo SK,French R,Spada F,Clarkson R

13-0611-81 was used in Flow cytometry/Cell sorting to indicate that inhibiting the processing of p100 may be a potential therapeutic strategy to suppress cancer stem cell (CSC) activity in a subset of breast tumours.

Sat Apr 01 00:00:00 EDT 2017

Opposing roles of Nfkb2 gene products p100 and p52 in the regulation of breast cancer stem cells.

Breast cancer research and treatment

Yeo SK,French R,Spada F,Clarkson R

Sat Apr 01 00:00:00 EDT 2017

Autophagy Differentially Regulates Distinct Breast Cancer Stem-like Cells in Murine Models via EGFR/Stat3 and Tgfβ/Smad Signaling.

Cancer research

Yeo SK,Wen J,Chen S,Guan JL

13-0611 was used in Flow cytometry/Cell sorting to identify the coexistence of distinct breast cancer stem-like cells based on their expression of ALDH(+) and CD29(hi)CD61(+) markers, which are differentially regulated by autophagy.

Wed Jun 01 00:00:00 EDT 2016

WISP1-αvβ3 integrin signaling positively regulates TLR-triggered inflammation response in sepsis induced lung injury.

Scientific reports

Chen Z,Ding X,Jin S,Pitt B,Zhang L,Billiar T,Li Q

Published figure using CD61 (Integrin beta 3) monoclonal antibody (Product # 13-0611-81) in Neutralization

Tue Jun 28 00:00:00 EDT 2016

WISP1-αvβ3 integrin signaling positively regulates TLR-triggered inflammation response in sepsis induced lung injury.

Scientific reports

Chen Z,Ding X,Jin S,Pitt B,Zhang L,Billiar T,Li Q

Published figure using CD61 (Integrin beta 3) monoclonal antibody (Product # 13-0611-81) in Neutralization

Tue Jun 28 00:00:00 EDT 2016

H2S protects against fatal myelosuppression by promoting the generation of megakaryocytes/platelets.

Journal of hematology & oncology

Liu HD,Zhang AJ,Xu JJ,Chen Y,Zhu YC

Published figure using CD61 (Integrin beta 3) monoclonal antibody (Product # 13-0611-81) in Immunofluorescence

Wed Feb 24 00:00:00 EST 2016

Integrin-modulating therapy prevents fibrosis and autoimmunity in mouse models of scleroderma.

Nature

Gerber EE,Gallo EM,Fontana SC,Davis EC,Wigley FM,Huso DL,Dietz HC

Published figure using CD61 (Integrin beta 3) monoclonal antibody (Product # 13-0611-81) in Flow Cytometry

Thu Nov 07 00:00:00 EST 2013

Notch2 genetic fate mapping reveals two previously unrecognized mammary epithelial lineages.

Nature cell biology

Šale S,Lafkas D,Artavanis-Tsakonas S

13-0611 was used in Flow cytometry/Cell sorting to study the fate of cells expressing the Notch2 receptor paralogue, uncovering two novel mammary epithelial cell linages.

Wed May 01 00:00:00 EDT 2013

Evidence for a multipotent mammary progenitor with pregnancy-specific activity.

Breast cancer research : BCR

Kaanta AS,Virtanen C,Selfors LM,Brugge JS,Neel BG

13-0611 was used in Flow cytometry/Cell sorting to provide data to show the existence of multi-potent mammary progenitor cells that have pregnancy-specific function.

Mon Dec 08 00:00:00 EST 2014

Evidence for a multipotent mammary progenitor with pregnancy-specific activity.

Breast cancer research : BCR

Kaanta AS,Virtanen C,Selfors LM,Brugge JS,Neel BG

13-0611 was used in Flow cytometry/Cell sorting to provide data to show the existence of multi-potent mammary progenitor cells that have pregnancy-specific function.

Mon Dec 08 00:00:00 EST 2014

β1 integrins with individually disrupted cytoplasmic NPxY motifs are embryonic lethal but partially active in the epidermis.

The Journal of investigative dermatology

Meves A,Stremmel C,Böttcher RT,Fässler R

Published figure using CD61 (Integrin beta 3) monoclonal antibody (Product # 13-0611-81) in Flow Cytometry

Sun Dec 01 00:00:00 EST 2013

Differentiation generates paracrine cell pairs that maintain basaloid mouse mammary tumors: proof of concept.

PloS one

Kim S,Goel S,Alexander CM

Tue Apr 26 00:00:00 EDT 2011

Plasminogen activator inhibitor-1 regulates integrin alphavbeta3 expression and autocrine transforming growth factor beta signaling.

The Journal of biological chemistry

Pedroja BS,Kang LE,Imas AO,Carmeliet P,Bernstein AM

Published figure using CD61 (Integrin beta 3) monoclonal antibody (Product # 13-0611-81) in Western Blot

Fri Jul 31 00:00:00 EDT 2009

Plasminogen activator inhibitor-1 regulates integrin alphavbeta3 expression and autocrine transforming growth factor beta signaling.

The Journal of biological chemistry

Pedroja BS,Kang LE,Imas AO,Carmeliet P,Bernstein AM

Published figure using CD61 (Integrin beta 3) monoclonal antibody (Product # 13-0611-81) in Western Blot

Fri Jul 31 00:00:00 EDT 2009

Constitutively active aryl hydrocarbon receptor expressed specifically in T-lineage cells causes thymus involution and suppresses the immunization-induced increase in splenocytes.

Journal of immunology (Baltimore, Md. : 1950)

Nohara K,Pan X,Tsukumo S,Hida A,Ito T,Nagai H,Inouye K,Motohashi H,Yamamoto M,Fujii-Kuriyama Y,Tohyama C

Tue Mar 01 00:00:00 EST 2005

Bioinformatics

Protein Aliases: CD61; GPIIIa; integrin beta 3 (Cd61); Integrin beta-3; platelet gpIIIa; Platelet membrane glycoprotein IIIa

View more View less

Gene Aliases: CD61; GP3A; INGRB3; Itgb3

View more View less

UniProt ID: (Rat) Q8R2H2, (Mouse) O54890

View more View less

Entrez Gene ID: (Rat) 29302, (Mouse) 16416

View more View less

Function(s)

fibronectin binding protease binding protein disulfide isomerase activity integrin binding protein binding peptide binding identical protein binding vascular endothelial growth factor receptor 2 binding cell adhesion molecule binding extracellular matrix binding fibrinogen binding receptor activity integrin

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

CD61 (Integrin beta 3) Monoclonal Antibody (2C9.G3), Biotin, eBioscience™

CD61 (Integrin beta 3) Antibody (13-0611-81) in Flow

Product Details

13-0611-81

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Neutralization (Neu)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!