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Invitrogen
CD61 (Integrin beta 3) Monoclonal Antibody (2C9.G3), Functional Grade, eBioscience™
-Functional-Grade-Flow-20170216130641.jpg?time=20220826 "CD61 (Integrin beta 3) Antibody in Flow Cytometry (Flow)")
-Functional-Grade-Flow-20170216130641.jpg?time=20220826)
-Functional-Grade-Flow-20170216130641.jpg?time=20220826?time=20220826 "CD61 (Integrin beta 3) Antibody (16-0611-82) in Flow")
Product Details
16-0611-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat
Published species
Human,
Mouse
Host/Isotype
Armenian hamster
/ IgG
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
2C9.G3
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
no preservative
Storage conditions
4° C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_468984
Product Specific Information
Description: The 2C9.G3 (HMb3-1) monoclonal antibody reacts with mouse and rat CD61, also known as the integrin beta3. CD61 is expressed by activated T cells, granulocytes, and platelet. CD61 associates non-covalently with two integrin alpha subunits; alphaV (CD51) to form Vitronectin Receptor and alphaIib (CD41) to form gpIIb/IIIa. These heterodimeric complexes are responsible for adhesion to extracellular matrix components including fibrinogen, fibronectin, fibronectin, vitronectin, thrombospondin and von Willebrand factor.
Applications Reported: The 2C9.G3 antibody has been reported for use in flow cytometric analysis. 2C9.G3 has also been reported in blocking of ligand binding and some adhesive processes.
Applications Tested: The 2C9.G3 antibody has been tested by flow cytometric analysis of mouse splenocytes and bone marrow cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Storage and handling: Use in a sterile environment.
Filtration: 0.2 µm post-manufacturing filtered.
Purity: Greater than 90%, as determined by SDS-PAGE.
Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.
Aggregation: Less than 10%, as determined by HPLC.
Target Information
CD61 (GPIIIa, ITGB3) is a glycoprotein found on megakaryocytes, platelets and their precursors. CD61 antigen plays a role in platelet aggregation and also as a receptor for fibrinogen, fibronectin, von Willebrand factor and vitronectrin. CD61 is a 105 kDa glycoprotein that associates with either the alpha 11b integrin (CD41) or the alpha V integrin (CD51) at the cell surface. CD61 is expressed on platelets and megakaryocytes in association with CD41, and on endothelial cells, monocytes, platelets and osteoclasts in association with CD51. CD61 is a receptor for fibrinogen, fibronectin, Von Willebrand Factor, vitronectin and thrombospondin. The CD61 protein product is composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. CD61 is found along with the alpha IIb chain in platelets. Like other integrins, CD61 is known to participate in cell adhesion as well as cell-surface mediated signaling. Diseases associated with CD61 dysfunction include Glanzmann Thrombasthenia and Platelet type-16 Bleeding Disorder.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CD61; GPIIIa; integrin beta 3 (Cd61); Integrin beta-3; platelet gpIIIa; Platelet membrane glycoprotein IIIa
Gene Aliases: CD61; GP3A; INGRB3; Itgb3
UniProt ID: (Rat) Q8R2H2, (Mouse) O54890
Entrez Gene ID: (Rat) 29302, (Mouse) 16416
fibronectin binding
protease binding
protein disulfide isomerase activity
integrin binding
protein binding
peptide binding
identical protein binding
vascular endothelial growth factor receptor 2 binding
cell adhesion molecule binding
extracellular matrix binding
fibrinogen binding
receptor activity
integrin
positive regulation of endothelial cell proliferation
positive regulation of leukocyte migration
protein folding
cellular calcium ion homeostasis
cell-substrate junction assembly
cell adhesion
cell-matrix adhesion
integrin-mediated signaling pathway
embryo implantation
regulation of G-protein coupled receptor protein signaling pathway
response to radiation
positive regulation of endothelial cell migration
negative regulation of macrophage derived foam cell differentiation
positive regulation of fibroblast migration
negative regulation of lipid storage
response to activity
smooth muscle cell migration
positive regulation of smooth muscle cell migration
cell growth
cell migration
platelet activation
regulation of cell migration
positive regulation of cell migration
cell-substrate adhesion
activation of protein kinase activity
negative regulation of lipid transport
positive regulation of cell adhesion mediated by integrin
positive regulation of osteoblast proliferation
heterotypic cell-cell adhesion
substrate adhesion-dependent cell spreading
cellular response to drug
response to platelet-derived growth factor
apolipoprotein A-I-mediated signaling pathway
positive regulation of MAPK cascade
negative regulation of low-density lipoprotein particle receptor biosynthetic process
positive regulation of angiogenesis
positive regulation of bone resorption
viral entry into host cell
mesodermal cell differentiation
positive regulation of smooth muscle cell proliferation
positive regulation of peptidyl-tyrosine phosphorylation
negative regulation of lipoprotein metabolic process
negative chemotaxis
regulation of release of sequestered calcium ion into cytosol
negative regulation of cell death
positive regulation of ERK1 and ERK2 cascade
platelet aggregation
cellular response to mechanical stimulus
positive regulation of glomerular mesangial cell proliferation
positive regulation of adenylate cyclase-inhibiting opioid receptor signaling pathway
cellular response to insulin-like growth factor stimulus
positive regulation of osteoclast differentiation
cell projection morphogenesis
angiogenesis involved in wound healing
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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