Invitrogen

IL-2 Monoclonal Antibody (JES6-5H4), FITC, eBioscience™

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

23 Published Figures

31 References

View all (144) IL-2 antibodies

Datasheet

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Datasheet

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FIGURE: 1 / 25

IL-2 Antibody (11-7021-82) in Flow

Mouse splenocytes were stimulated with PMA/Ionomycin in the presence of Monensin (Product # 00-4505-51) for 5 hours. The cells were surface stained with Anti-Mouse CD3e PE (Product # 12-0031-82) and intracellularly stained with 0.25 µg of Rat IgG2b K Isotype Control FITC (Product # 11-4031-82) (left) or 0.25 µg of Anti-Mouse IL-2 FITC (right).

Product Image Gallery

IL-2 Antibody — Intracellular staining of stimulated mouse splenocytes. As expected based on known expression patterns, IL-2 clone JES6-5H4 stains a large subset of CD4+ T cells and a minor subset of NK1.1+ NK cells with no staining observed without stimulation. Details: Mouse splenocytes were cultured in the presence of Protein Transport Inhibitors (500X) (Unstimulated, bottom row) or Cell Stimulation Cocktail (plus protein transport inhibitors, 500X) for 5 hours (Stimulated, top row). Cells were fixed and permeabilized with the IC Fixation & Permeabilization Buffer Set and protocol followed by intracellular staining with CD4 (clone RM4-5), NK1.1 (clone PK136) and IL-2 (clone JES6-5H4). Cells in the CD4+ (blue histogram) or NK1.1+ (purple histogram) gates were used for analysis. {TM}

Product Details

11-7021-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.5 µg/test

View 27 publications 27 publications

ELISA (ELISA)

View 4 publications 4 publications

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host/Isotype

Rat / IgG2b, kappa

Recommended Isotype Control

Rat IgG2b kappa Isotype Control (eB149/10H5), FITC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

JES6-5H4

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_465382

Product Specific Information

Description: The JES6-5H4 antibody reacts with mouse interleukin-2 (IL-2), a 17 kDa T cell growth factor and a major immunoregulatory cytokine.

Applications Reported:JES6-5H4 has been reported for use in intracellular staining and flow cytometric analysis.

Applications Tested: Has been tested by intracellular staining and flow cytometric analysis and can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Interleukin 2 (IL-2) is an immuno-modulatory cytokine that is important for the proliferation of activated T cells, differentiation of B cells, natural killer cells, monocytes and macrophages. IL-2 signals through the IL-2 receptor (IL-2R), a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL-4) and interleukin 7 (IL-7). The expression of the IL-2 gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a gene similar to IL-2 in mice leads to an ulcerative colitis-like disease that suggests an essential role of this gene in the immune response to antigenic stimuli.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Forsythiaside A Improves the Inhibitory Efficiency of Recombinant Protein Vaccines against Bovine Viral Diarrhea Virus Infection.

International journal of molecular sciences

Yang G,Wang J,Wang S,Zhu Y

Published figure using IL-2 monoclonal antibody (Product # 11-7021-82) in Flow Cytometry

Sat Aug 20 00:00:00 EDT 2022

In vivo genome-wide CRISPR screening identifies ZNF24 as a negative NF-κB modulator in lung cancer.

Cell & bioscience

Liu L,Lei Y,Chen W,Zhou Q,Zheng Z,Zeng G,Liu W,Feng P,Zhang Z,Yu L,Chen L

Published figure using IL-2 monoclonal antibody (Product # 11-7021-82) in Flow Cytometry

Thu Dec 01 00:00:00 EST 2022

Z. morio Hemolymph Relieves E. coli-Induced Mastitis by Inhibiting Inflammatory Response and Repairing the Blood-Milk Barrier.

International journal of molecular sciences

Zou Y,Wang X,Xu J,Wang S,Li S,Zhu Y,Wang J

Published figure using IL-2 monoclonal antibody (Product # 11-7021-82) in Flow Cytometry

Mon Oct 31 00:00:00 EDT 2022

Impaired Treg-DC interactions contribute to autoimmunity in leukocyte adhesion deficiency type 1.

JCI insight

Klaus T,Wilson AS,Vicari E,Hadaschik E,Klein M,Helbich SSC,Kamenjarin N,Hodapp K,Schunke J,Haist M,Butsch F,Probst HC,Enk AH,Mahnke K,Waisman A,Bednarczyk M,Bros M,Bopp T,Grabbe S

Published figure using IL-2 monoclonal antibody (Product # 11-7021-82) in Flow Cytometry

Thu Dec 22 00:00:00 EST 2022

CRISPR screens unveil signal hubs for nutrient licensing of T cell immunity.

Nature

Long L,Wei J,Lim SA,Raynor JL,Shi H,Connelly JP,Wang H,Guy C,Xie B,Chapman NM,Fu G,Wang Y,Huang H,Su W,Saravia J,Risch I,Wang YD,Li Y,Niu M,Dhungana Y,Kc A,Zhou P,Vogel P,Yu J,Pruett-Miller SM,Peng J,Chi H

11-7021-82 was used in Flow cytometry/Cell sorting to establish epigenetic and post-translational mechanisms that underpin how nutrient transporters, sensors and transducers interplay with immune signals for three-tiered regulation of mTORC1 activity and identify their pivotal roles in licensing T cell immunity and immune tolerance.

Wed Dec 01 00:00:00 EST 2021

A Methyltransferase-Defective Vesicular Stomatitis Virus-Based SARS-CoV-2 Vaccine Candidate Provides Complete Protection against SARS-CoV-2 Infection in Hamsters.

Journal of virology

Lu M,Zhang Y,Dravid P,Li A,Zeng C,Kc M,Trivedi S,Sharma H,Chaiwatpongsakorn S,Zani A,Kenney A,Cai C,Ye C,Liang X,Qiu J,Martinez-Sobrido L,Yount JS,Boyaka PN,Liu SL,Peeples ME,Kapoor A,Li J

Published figure using IL-2 monoclonal antibody (Product # 11-7021-82) in Flow Cytometry

Mon Sep 27 00:00:00 EDT 2021

Local Sustained GM-CSF Delivery by Genetically Engineered Encapsulated Cells Enhanced Both Cellular and Humoral SARS-CoV-2 Spike-Specific Immune Response in an Experimental Murine Spike DNA Vaccination Model.

Vaccines

Vernet R,Charrier E,Cosset E,Fièvre S,Tomasello U,Grogg J,Mach N

Published figure using IL-2 monoclonal antibody (Product # 11-7021-82) in Flow Cytometry

Mon May 10 00:00:00 EDT 2021

Blocking interaction between SHP2 and PD-1 denotes a novel opportunity for developing PD-1 inhibitors.

EMBO molecular medicine

Fan Z,Tian Y,Chen Z,Liu L,Zhou Q,He J,Coleman J,Dong C,Li N,Huang J,Xu C,Zhang Z,Gao S,Zhou P,Ding K,Chen L

Published figure using IL-2 monoclonal antibody (Product # 11-7021-82) in Flow Cytometry

Mon Jun 08 00:00:00 EDT 2020

Deletion Timing of Cic Alleles during Hematopoiesis Determines the Degree of Peripheral CD4⁺ T Cell Activation and Proliferation.

Immune network

Park GY,Lee GW,Kim S,Hong H,Park JS,Cho JH,Lee Y

Published figure using IL-2 monoclonal antibody (Product # 11-7021-82) in ELISA

Thu Oct 01 00:00:00 EDT 2020

Synthetic DNA Vaccines Adjuvanted with pIL-33 Drive Liver-Localized T Cells and Provide Protection from Plasmodium Challenge in a Mouse Model.

Vaccines

Reeder SM,Reuschel EL,Bah MA,Yun K,Tursi NJ,Kim KY,Chu J,Zaidi FI,Yilmaz I,Hart RJ,Perrin B,Xu Z,Humeau L,Weiner DB,Aly ASI

Published figure using IL-2 monoclonal antibody (Product # 11-7021-82) in Flow Cytometry

Fri Jan 10 00:00:00 EST 2020

Deletion of immune evasion genes provides an effective vaccine design for tumor-associated herpesviruses.

NPJ vaccines

Brar G,Farhat NA,Sukhina A,Lam AK,Kim YH,Hsu T,Tong L,Lin WW,Ware CF,Blackman MA,Sun R,Wu TT

Published figure using IL-2 monoclonal antibody (Product # 11-7021-82) in Flow Cytometry

Thu Nov 05 00:00:00 EST 2020

Therapeutic Potential of Targeting Malt1-Dependent TCR Downstream Signaling to Promote the Survival of MHC-Mismatched Allografts.

Frontiers in immunology

Govender L,Mikulic J,Wyss JC,Gaide O,Thome M,Golshayan D

11-7021-82 was used in Flow Cytometry to compare targeting Malt1 versus using calcineurin inhibitors as immunosuppression in a stringent experimental transplantation model.

Fri Jun 18 00:00:00 EDT 2021

Inactivation of TRPM7 kinase in mice results in enlarged spleens, reduced T-cell proliferation and diminished store-operated calcium entry.

Scientific reports

Beesetty P,Wieczerzak KB,Gibson JN,Kaitsuka T,Luu CT,Matsushita M,Kozak JA

11-7021 was used in Flow cytometry/Cell sorting to study the role of TRPM7 channel kinase as a novel modulator of blastogenesis, proliferation and store-operated calcium entry.

Wed Feb 14 00:00:00 EST 2018

Excessive expression of miR-27 impairs Treg-mediated immunological tolerance.

The Journal of clinical investigation

Cruz LO,Hashemifar SS,Wu CJ,Cho S,Nguyen DT,Lin LL,Khan AA,Lu LF

Published figure using IL-2 monoclonal antibody (Product # 11-7021-82) in Flow Cytometry

Wed Feb 01 00:00:00 EST 2017

Vaccination with High-Affinity Epitopes Impairs Antitumor Efficacy by Increasing PD-1 Expression on CD8⁺ T Cells.

Cancer immunology research

Zahm CD,Colluru VT,McNeel DG

Published figure using IL-2 monoclonal antibody (Product # 11-7021-82) in Flow Cytometry

Tue Aug 01 00:00:00 EDT 2017

A New Adjuvant MTOM Mediates Mycobacterium tuberculosis Subunit Vaccine to Enhance Th1-Type T Cell Immune Responses and IL-2⁺ T Cells.

Frontiers in immunology

Yu Q,Wang X,Fan X

Published figure using IL-2 monoclonal antibody (Product # 11-7021-82) in Flow Cytometry

Wed Nov 20 00:00:00 EST 2019

Capicua deficiency induces autoimmunity and promotes follicular helper T cell differentiation via derepression of ETV5.

Nature communications

Park S,Lee S,Lee CG,Park GY,Hong H,Lee JS,Kim YM,Lee SB,Hwang D,Choi YS,Fryer JD,Im SH,Lee SW,Lee Y

Published figure using IL-2 monoclonal antibody (Product # 11-7021-82) in ELISA

Wed Jul 12 00:00:00 EDT 2017

Tristetraprolin inhibits macrophage IL-27-induced activation of antitumour cytotoxic T cell responses.

Nature communications

Wang Q,Ning H,Peng H,Wei L,Hou R,Hoft DF,Liu J

Published figure using IL-2 monoclonal antibody (Product # 11-7021-82) in Flow Cytometry

Wed Oct 11 00:00:00 EDT 2017

A Multistage Subunit Vaccine Effectively Protects Mice Against Primary Progressive Tuberculosis, Latency and Reactivation.

EBioMedicine

Ma J,Teng X,Wang X,Fan X,Wu Y,Tian M,Zhou Z,Li L

Published figure using IL-2 monoclonal antibody (Product # 11-7021-82) in Flow Cytometry

Tue Aug 01 00:00:00 EDT 2017

G-CSF-Induced Suppressor IL-10+ Neutrophils Promote Regulatory T Cells That Inhibit Graft-Versus-Host Disease in a Long-Lasting and Specific Way.

Journal of immunology (Baltimore, Md. : 1950)

Perobelli SM,Mercadante AC,Galvani RG,Gonçalves-Silva T,Alves AP,Pereira-Neves A,Benchimol M,Nóbrega A,Bonomo A

11-7021 was used in Flow cytometry/Cell sorting to study the role of incoming IL-10+ neutrophils from G-CSF-treated donor spleen in inhibiting acute graft-versus-host disease.

Tue Nov 01 00:00:00 EDT 2016

Alternative splicing of MALT1 controls signalling and activation of CD4(+) T cells.

Nature communications

Meininger I,Griesbach RA,Hu D,Gehring T,Seeholzer T,Bertossi A,Kranich J,Oeckinghaus A,Eitelhuber AC,Greczmiel U,Gewies A,Schmidt-Supprian M,Ruland J,Brocker T,Heissmeyer V,Heyd F,Krappmann D

11-7021 was used in Flow cytometry/Cell sorting to study how TCR-induced alternative splicing augments MALT1 scaffolding to enhance downstream signalling and to promote optimal T-cell activation.

Tue Apr 12 00:00:00 EDT 2016

Immunogenicity and protective efficacy of DMT liposome-adjuvanted tuberculosis subunit CTT3H vaccine.

Human vaccines & immunotherapeutics

Teng X,Tian M,Li J,Tan S,Yuan X,Yu Q,Jing Y,Zhang Z,Yue T,Zhou L,Fan X

Published figure using IL-2 monoclonal antibody (Product # 11-7021-82) in Flow Cytometry

Fri Mar 11 00:00:00 EST 2016

TLR2 engagement on CD4(+) T cells enhances effector functions and protective responses to Mycobacterium tuberculosis.

European journal of immunology

Reba SM,Li Q,Onwuzulike S,Ding X,Karim AF,Hernandez Y,Fulton SA,Harding CV,Lancioni CL,Nagy N,Rodriguez ME,Wearsch PA,Rojas RE

Published figure using IL-2 monoclonal antibody (Product # 11-7021-82) in Flow Cytometry

Thu May 01 00:00:00 EDT 2014

Mycobacterial membrane vesicles administered systemically in mice induce a protective immune response to surface compartments of Mycobacterium tuberculosis.

mBio

Prados-Rosales R,Carreño LJ,Batista-Gonzalez A,Baena A,Venkataswamy MM,Xu J,Yu X,Wallstrom G,Magee DM,LaBaer J,Achkar JM,Jacobs WR,Chan J,Porcelli SA,Casadevall A

Tue Sep 30 00:00:00 EDT 2014

Chemoimmunotherapy reduces the progression of multiple myeloma in a mouse model.

Cancer prevention research (Philadelphia, Pa.)

Sharabi A,Laronne-Bar-On A,Meshorer A,Haran-Ghera N

11-7021 was used in Flow cytometry/Cell sorting to determine the effects, and their modes of action, of CYC treatment on MM progression.

Fri Oct 01 00:00:00 EDT 2010

Enhancement of immunogenicity of a therapeutic cervical cancer DNA-based vaccine by co-application of sequence-optimized genetic adjuvants.

International journal of cancer

Ohlschläger P,Quetting M,Alvarez G,Dürst M,Gissmann L,Kaufmann AM

11-7021 was used in Flow cytometry/Cell sorting to suggest that adding adjuvant genes may be enhance synergistic immune responses by DNA immunization for the treatment of cervical cancer.

Wed Jul 01 00:00:00 EDT 2009

Tc17, a unique subset of CD8 T cells that can protect against lethal influenza challenge.

Journal of immunology (Baltimore, Md. : 1950)

Hamada H,Garcia-Hernandez Mde L,Reome JB,Misra SK,Strutt TM,McKinstry KK,Cooper AM,Swain SL,Dutton RW

11-7021 was used in Flow cytometry/Cell sorting to study the role of Tc17 cells in influenza infection, showing that they protect against lethality.

Sun Mar 15 00:00:00 EDT 2009

Therapeutic effect of a T helper cell supported CTL response induced by a survivin peptide vaccine against murine cerebral glioma.

Cancer immunology, immunotherapy : CII

Ciesielski MJ,Kozbor D,Castanaro CA,Barone TA,Fenstermaker RA

11-7021 was used in Flow cytometry/Cell sorting to identify survivin epitopes that might serve as targets for CTL-mediated, anti-tumour responses.

Mon Dec 01 00:00:00 EST 2008

Single-dose protection against Plasmodium berghei by a simian adenovirus vector using a human cytomegalovirus promoter containing intron A.

Journal of virology

Sridhar S,Reyes-Sandoval A,Draper SJ,Moore AC,Gilbert SC,Gao GP,Wilson JM,Hill AV

11-7021 was used in Flow cytometry/Cell sorting to compare efficacy of AdH5 and AdC6 vector vaccines expressing antigen under HCMV promotion.

Tue Apr 01 00:00:00 EDT 2008

Modulation of pulmonary dendritic cell function during mycobacterial infection.

Infection and immunity

Anis MM,Fulton SA,Reba SM,Liu Y,Harding CV,Boom WH

Published figure using IL-2 monoclonal antibody (Product # 11-7021-82) in ELISA

Fri Feb 01 00:00:00 EST 2008

Modulation of naive CD4+ T-cell responses to an airway antigen during pulmonary mycobacterial infection.

Infection and immunity

Anis MM,Fulton SA,Reba SM,Harding CV,Boom WH

Published figure using IL-2 monoclonal antibody (Product # 11-7021-82) in ELISA

Tue May 01 00:00:00 EDT 2007

Bioinformatics

Protein Aliases: H-IL-2; il 2; IL-2; ILN; Interleukin; Interleukin-2; Interleukin2; Involved in regulation of T cell clonal expansion; M-IL-2; T-cell growth factor; T-cell growth factor (TCGF); TCGF

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Gene Aliases: Il-2; Il2

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UniProt ID: (Mouse) P04351

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Entrez Gene ID: (Mouse) 16183

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Function(s)

cytokine activity interleukin-2 receptor binding growth factor activity carbohydrate binding kappa-type opioid receptor binding glycosphingolipid binding actin or actin-binding cytoskeletal protein non-receptor serine/threonine protein kinase

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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IL-2 Monoclonal Antibody (JES6-5H4), FITC, eBioscience™

IL-2 Antibody (11-7021-82) in Flow

Product Details

11-7021-82

Flow Cytometry (Flow)

ELISA (ELISA)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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