Invitrogen

CD20 Monoclonal Antibody (2H7), Biotin, eBioscience™

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Antibody Testing Data (1)
Published Figures (16)

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CD20 Antibody (13-0209-82) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD19 APC (Product # 17-0199-42) and 0.125 µg of Mouse IgG2b K Isotype Control Biotin (Product # 13-4732-85) (left) or 0.125 µg of Anti-Human CD20 Biotin (right) followed by Streptavidin PE (Product # 12-4317-87). Cells in the lymphocyte gate were used for analysis.

CD20 Antibody in Immunohistochemistry (IHC)

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Product Details

13-0209-82

Applications

Tested Dilution

Publications

Western Blot (WB)

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunohistochemistry (Frozen) (IHC (F))

Assay-Dependent

Flow Cytometry (Flow)

0.25 µg/test

View 13 publications 13 publications

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG2b, kappa

Recommended Isotype Control

Mouse IgG2b kappa Isotype Control (eBMG2b), Biotin, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

2H7

Conjugate

Biotin Biotin Biotin

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_657690

Product Specific Information

Description: The 2H7 monoclonal antibody reacts with human CD20, a 33-36 kDa transmembrane protein. CD20 is expressed by developing B cells as well as mature B cells but not plasma cells. CD20 has been detected at low levels on a small subset of mature T cells. It is suggested that CD20 plays a role in B-cell activation.

Applications Reported: This 2H7 antibody has been reported for use in flow cytometric analysis and immunohistology staining of frozen tissue sections.

Applications Tested: This 2H7 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD20 is a non-glycosylated surface phosphoprotein that has a molecular weight range of 33-37 kDa depending on the degree of phosphorylation. CD20 is expressed on mature and most malignant B cells, in a subpopulation of T lymphocytes and follicular dendritic cells. CD20 expression on B cells is synchronous with the expression of surface IgM and it regulates transmembrane calcium conductance, cell cycle progression and B-cell proliferation. CD20 is also associated with lipid rafts, but the intensity of this association depends on extracellular triggering, employing CD20 conformational change, and/or BCR (B cell antigen receptor) aggregation. After the receptor ligation, BCR and CD20 colocalize and then rapidly dissociate before BCR endocytosis, whereas CD20 remains at the cell surface. CD20 serves as a useful target for antibody-mediated therapeutic depletion of B cells, as it is expressed at high levels on most B-cell malignancies, but does not become internalized or shed from the plasma membrane following monoclonal antibody treatment. Diseases associated with CD20 dysfunction include Ms4a1-related common variable immune deficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Spontaneous EBV-Reactivation during B Cell Differentiation as a Model for Polymorphic EBV-Driven Lymphoproliferation.

Cancers

Care MA,Stephenson S,Owen R,Doody GM,Tooze RM

Published figure using CD20 monoclonal antibody (Product # 13-0209-82) in Flow Cytometry

Wed Jun 07 00:00:00 EDT 2023

A Soluble NK-CAR Mediates the Specific Cytotoxicity of NK Cells toward the Target CD20⁺ Lymphoma Cells.

Aging and disease

Liu R,Luo Q,Luo W,Wan L,Zhu Q,Yin X,Lu X,Song Z,Wei L,Xiang Z,Zou Y

Published figure using CD20 monoclonal antibody (Product # 13-0209-82) in Flow Cytometry

Sat Oct 01 00:00:00 EDT 2022

Heritable vaginal bacteria influence immune tolerance and relate to early-life markers of allergic sensitization in infancy.

Cell reports. Medicine

McCauley KE,Rackaityte E,LaMere B,Fadrosh DW,Fujimura KE,Panzer AR,Lin DL,Lynch KV,Halkias J,Mendoza VF,Burt TD,Bendixsen C,Barnes K,Kim H,Jones K,Ownby DR,Johnson CC,Seroogy CM,Gern JE,Boushey HA,Lynch SV

13-0209-82 was used in Flow cytometry/Cell sorting to investigate if bacterial and atopy heritability are intimately linked.

Tue Aug 16 00:00:00 EDT 2022

TRPA1 triggers hyperalgesia and inflammation after tooth bleaching.

Scientific reports

Chen C,Huang X,Zhu W,Ding C,Huang P,Li R

Published figure using CD20 monoclonal antibody (Product # 13-0209-82) in Flow Cytometry

Tue Aug 31 00:00:00 EDT 2021

IL-33 Alarmin and Its Active Proinflammatory Fragments Are Released in Small Intestine in Celiac Disease.

Frontiers in immunology

Perez F,Ruera CN,Miculan E,Carasi P,Dubois-Camacho K,Garbi L,Guzman L,Hermoso MA,Chirdo FG

Published figure using CD20 monoclonal antibody (Product # 13-0209-82) in Immunohistochemistry

Tue Jun 22 00:00:00 EDT 2021

CD19-targeted CAR regulatory T cells suppress B cell pathology without GvHD.

JCI insight

Imura Y,Ando M,Kondo T,Ito M,Yoshimura A

13-0209 was used in Flow cytometry/Cell sorting to suggest that CD19-CAR regulatory T cells can treat autoantibody-mediated autoimmune diseases by suppressing B cell pathology.

Thu Jul 23 00:00:00 EDT 2020

Relative safety of various spermatogenic stem cell purification methods for application in spermatogenic stem cell transplantation.

Stem cell research & therapy

Tian J,Ma K,Pei CB,Zhang SH,Li X,Zhou Y,Yan B,Wang HY,Ma LH

Published figure using CD20 monoclonal antibody (Product # 13-0209-82) in Flow Cytometry

Mon Dec 16 00:00:00 EST 2019

Relative safety of various spermatogenic stem cell purification methods for application in spermatogenic stem cell transplantation.

Stem cell research & therapy

Tian J,Ma K,Pei CB,Zhang SH,Li X,Zhou Y,Yan B,Wang HY,Ma LH

Published figure using CD20 monoclonal antibody (Product # 13-0209-82) in Flow Cytometry

Mon Dec 16 00:00:00 EST 2019

Relative safety of various spermatogenic stem cell purification methods for application in spermatogenic stem cell transplantation.

Stem cell research & therapy

Tian J,Ma K,Pei CB,Zhang SH,Li X,Zhou Y,Yan B,Wang HY,Ma LH

Published figure using CD20 monoclonal antibody (Product # 13-0209-82) in Flow Cytometry

Mon Dec 16 00:00:00 EST 2019

Genetic modification of primary human B cells to model high-grade lymphoma.

Nature communications

Caeser R,Di Re M,Krupka JA,Gao J,Lara-Chica M,Dias JML,Cooke SL,Fenner R,Usheva Z,Runge HFP,Beer PA,Eldaly H,Pak HK,Park CS,Vassiliou GS,Huntly BJP,Mupo A,Bashford-Rogers RJM,Hodson DJ

Published figure using CD20 monoclonal antibody (Product # 13-0209-82) in Flow Cytometry

Fri Oct 04 00:00:00 EDT 2019

Evidence for persistence of the SHIV reservoir early after MHC haploidentical hematopoietic stem cell transplantation.

Nature communications

Colonna L,Peterson CW,Schell JB,Carlson JM,Tkachev V,Brown M,Yu A,Reddy S,Obenza WM,Nelson V,Polacino PS,Mack H,Hu SL,Zeleski K,Hoffman M,Olvera J,Furlan SN,Zheng H,Taraseviciute A,Hunt DJ,Betz K,Lane JF,Vogel K,Hotchkiss CE,Moats C,Baldessari A,Murnane RD,English C,Astley CA,Wangari S,Agricola B,Ahrens J,Iwayama N,May A,Stensland L,Huang MW,Jerome KR,Kiem HP,Kean LS

Published figure using CD20 monoclonal antibody (Product # 13-0209-82) in Flow Cytometry

Thu Oct 25 00:00:00 EDT 2018

Tumor-associated B-cells induce tumor heterogeneity and therapy resistance.

Nature communications

Somasundaram R,Zhang G,Fukunaga-Kalabis M,Perego M,Krepler C,Xu X,Wagner C,Hristova D,Zhang J,Tian T,Wei Z,Liu Q,Garg K,Griss J,Hards R,Maurer M,Hafner C,Mayerhöfer M,Karanikas G,Jalili A,Bauer-Pohl V,Weihsengruber F,Rappersberger K,Koller J,Lang R,Hudgens C,Chen G,Tetzlaff M,Wu L,Frederick DT,Scolyer RA,Long GV,Damle M,Ellingsworth C,Grinman L,Choi H,Gavin BJ,Dunagin M,Raj A,Scholler N,Gross L,Beqiri M,Bennett K,Watson I,Schaider H,Davies MA,Wargo J,Czerniecki BJ,Schuchter L,Herlyn D,Flaherty K,Herlyn M,Wagner SN

Published figure using CD20 monoclonal antibody (Product # 13-0209-82) in Immunofluorescence

Tue Sep 19 00:00:00 EDT 2017

Tumor-associated B-cells induce tumor heterogeneity and therapy resistance.

Nature communications

Published figure using CD20 monoclonal antibody (Product # 13-0209-82) in Immunofluorescence

Tue Sep 19 00:00:00 EDT 2017

Phase I study of a bispecific ligand-directed toxin targeting CD22 and CD19 (DT2219) for refractory B-cell malignancies.

Clinical cancer research : an official journal of the American Association for Cancer Research

Bachanova V,Frankel AE,Cao Q,Lewis D,Grzywacz B,Verneris MR,Ustun C,Lazaryan A,McClune B,Warlick ED,Kantarjian H,Weisdorf DJ,Miller JS,Vallera DA

Published figure using CD20 monoclonal antibody (Product # 13-0209-82) in Flow Cytometry

Sun Mar 15 00:00:00 EDT 2015

Sexual Differences in Cell Loss during the Post-Hatch Development of Song Control Nuclei in the Bengalese Finch.

PloS one

Chen X,Li J,Zeng L,Zhang X,Lu X,Zuo M,Zhang X,Zeng S

Published figure using CD20 monoclonal antibody (Product # 13-0209-82) in Immunohistochemistry

Mon Apr 04 00:00:00 EDT 2016

Mcl-1 dependence predicts response to vorinostat and gemtuzumab ozogamicin in acute myeloid leukemia.

Leukemia research

Pierceall WE,Lena RJ,Medeiros BC,Blake N,Doykan C,Elashoff M,Cardone MH,Walter RB

13-0209 was used in Flow cytometry/Cell sorting to investigate whether BH3 profiling of apoptotic functionality could predict outcomes following treatment with vorinosta and gemtuzumab ozogamicin.

Thu May 01 00:00:00 EDT 2014

Novel immunomodulators from hard ticks selectively reprogramme human dendritic cell responses.

PLoS pathogens

Preston SG,Majtán J,Kouremenou C,Rysnik O,Burger LF,Cabezas Cruz A,Chiong Guzman M,Nunn MA,Paesen GC,Nuttall PA,Austyn JM

13-0209 was used in Binding experiments to provide a previously unknown mechanism for parasite-induced subversion of adaptive immunity.

Fri Jan 17 00:00:00 EST 2014

An efficient low cost method for gene transfer to T lymphocytes.

PloS one

Chicaybam L,Sodre AL,Curzio BA,Bonamino MH

Published figure using CD20 monoclonal antibody (Product # 13-0209-82) in Flow Cytometry

Mon Sep 30 00:00:00 EDT 2013

Transcriptional profiling of mRNAs and microRNAs in human bone marrow precursor B cells identifies subset- and age-specific variations.

PloS one

Jensen K,Brusletto BS,Aass HC,Olstad OK,Kierulf P,Gautvik KM

Published figure using CD20 monoclonal antibody (Product # 13-0209-82) in Flow Cytometry

Tue Apr 01 00:00:00 EDT 2014

Bioinformatics

Protein Aliases: APY; ATOPY; B-lymphocyte antigen CD20; B-lymphocyte cell-surface antigen B1; B-lymphocyte surface antigen B1; Bp35; CD20; CD20 antigen; CD20 receptor; Fc epsilon receptor I beta chain; Fc Fragment of IgE high affinity I receptor for beta polypeptide; FCER1B; IGEL; IGER; IGHER; LEU16; Leukocyte surface antigen Leu-16; Ly44; Membrane-spanning 4-domains subfamily A member 1; membrane-spanning 4-domains, subfamily A, member 1; MGC3969

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Gene Aliases: B1; Bp35; CD20; CVID5; LEU-16; MS4A1; MS4A2; S7

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UniProt ID: (Human) P11836

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Entrez Gene ID: (Human) 931

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Function(s)

epidermal growth factor receptor binding protein binding MHC class II protein complex binding transporter

Process(es)

humoral immune response B cell proliferation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD20 Monoclonal Antibody (2H7), Biotin, eBioscience™

CD20 Antibody (13-0209-82) in Flow

Product Details

13-0209-82

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Frozen) (IHC (F))

Flow Cytometry (Flow)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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