Invitrogen

CD20 Monoclonal Antibody (2H7), APC, eBioscience™

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Antibody Testing Data (1)
Published Figures (11)

FIGURE: 1 / 12

CD20 Antibody (17-0209-42) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD3 FITC (Product # 11-0038-42) and Mouse IgG2b K Isotype Control APC (Product # 17-4732-81) (left) or Anti-Human CD20 APC (right). Cells in the lymphocyte gate were used for analysis.

CD20 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

Product Details

17-0209-42

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Flow Cytometry (Flow)

5 µL (0.06 µg)/test

View 21 publications 21 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG2b, kappa

Recommended Isotype Control

Mouse IgG2b kappa Isotype Control (eBMG2b), APC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

2H7

Conjugate

APC APC APC

Excitation/Emission Max

651/660 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10670628

Product Specific Information

Description: The 2H7 monoclonal antibody reacts with human CD20, a 33-36 kDa transmembrane protein. CD20 is expressed by developing B cells as well as mature B cells but not plasma cells. CD20 has been detected at low levels on a small subset of mature T cells. It is suggested that CD20 plays a role in B-cell activation.

Applications Reported: This 2H7 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 2H7 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD20 is a non-glycosylated surface phosphoprotein that has a molecular weight range of 33-37 kDa depending on the degree of phosphorylation. CD20 is expressed on mature and most malignant B cells, in a subpopulation of T lymphocytes and follicular dendritic cells. CD20 expression on B cells is synchronous with the expression of surface IgM and it regulates transmembrane calcium conductance, cell cycle progression and B-cell proliferation. CD20 is also associated with lipid rafts, but the intensity of this association depends on extracellular triggering, employing CD20 conformational change, and/or BCR (B cell antigen receptor) aggregation. After the receptor ligation, BCR and CD20 colocalize and then rapidly dissociate before BCR endocytosis, whereas CD20 remains at the cell surface. CD20 serves as a useful target for antibody-mediated therapeutic depletion of B cells, as it is expressed at high levels on most B-cell malignancies, but does not become internalized or shed from the plasma membrane following monoclonal antibody treatment. Diseases associated with CD20 dysfunction include Ms4a1-related common variable immune deficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Spontaneous EBV-Reactivation during B Cell Differentiation as a Model for Polymorphic EBV-Driven Lymphoproliferation.

Cancers

Care MA,Stephenson S,Owen R,Doody GM,Tooze RM

Published figure using CD20 monoclonal antibody (Product # 17-0209-42) in Flow Cytometry

Wed Jun 07 00:00:00 EDT 2023

A Soluble NK-CAR Mediates the Specific Cytotoxicity of NK Cells toward the Target CD20⁺ Lymphoma Cells.

Aging and disease

Liu R,Luo Q,Luo W,Wan L,Zhu Q,Yin X,Lu X,Song Z,Wei L,Xiang Z,Zou Y

Published figure using CD20 monoclonal antibody (Product # 17-0209-42) in Flow Cytometry

Sat Oct 01 00:00:00 EDT 2022

TRPA1 triggers hyperalgesia and inflammation after tooth bleaching.

Scientific reports

Chen C,Huang X,Zhu W,Ding C,Huang P,Li R

Published figure using CD20 monoclonal antibody (Product # 17-0209-42) in Flow Cytometry

Tue Aug 31 00:00:00 EDT 2021

IL-33 Alarmin and Its Active Proinflammatory Fragments Are Released in Small Intestine in Celiac Disease.

Frontiers in immunology

Perez F,Ruera CN,Miculan E,Carasi P,Dubois-Camacho K,Garbi L,Guzman L,Hermoso MA,Chirdo FG

Published figure using CD20 monoclonal antibody (Product # 17-0209-42) in Immunohistochemistry

Tue Jun 22 00:00:00 EDT 2021

Relative safety of various spermatogenic stem cell purification methods for application in spermatogenic stem cell transplantation.

Stem cell research & therapy

Tian J,Ma K,Pei CB,Zhang SH,Li X,Zhou Y,Yan B,Wang HY,Ma LH

Published figure using CD20 monoclonal antibody (Product # 17-0209-42) in Flow Cytometry

Mon Dec 16 00:00:00 EST 2019

Genetic modification of primary human B cells to model high-grade lymphoma.

Nature communications

Caeser R,Di Re M,Krupka JA,Gao J,Lara-Chica M,Dias JML,Cooke SL,Fenner R,Usheva Z,Runge HFP,Beer PA,Eldaly H,Pak HK,Park CS,Vassiliou GS,Huntly BJP,Mupo A,Bashford-Rogers RJM,Hodson DJ

Published figure using CD20 monoclonal antibody (Product # 17-0209-42) in Flow Cytometry

Fri Oct 04 00:00:00 EDT 2019

Evidence for persistence of the SHIV reservoir early after MHC haploidentical hematopoietic stem cell transplantation.

Nature communications

Colonna L,Peterson CW,Schell JB,Carlson JM,Tkachev V,Brown M,Yu A,Reddy S,Obenza WM,Nelson V,Polacino PS,Mack H,Hu SL,Zeleski K,Hoffman M,Olvera J,Furlan SN,Zheng H,Taraseviciute A,Hunt DJ,Betz K,Lane JF,Vogel K,Hotchkiss CE,Moats C,Baldessari A,Murnane RD,English C,Astley CA,Wangari S,Agricola B,Ahrens J,Iwayama N,May A,Stensland L,Huang MW,Jerome KR,Kiem HP,Kean LS

Published figure using CD20 monoclonal antibody (Product # 17-0209-42) in Flow Cytometry

Thu Oct 25 00:00:00 EDT 2018

Tumor-associated B-cells induce tumor heterogeneity and therapy resistance.

Nature communications

Somasundaram R,Zhang G,Fukunaga-Kalabis M,Perego M,Krepler C,Xu X,Wagner C,Hristova D,Zhang J,Tian T,Wei Z,Liu Q,Garg K,Griss J,Hards R,Maurer M,Hafner C,Mayerhöfer M,Karanikas G,Jalili A,Bauer-Pohl V,Weihsengruber F,Rappersberger K,Koller J,Lang R,Hudgens C,Chen G,Tetzlaff M,Wu L,Frederick DT,Scolyer RA,Long GV,Damle M,Ellingsworth C,Grinman L,Choi H,Gavin BJ,Dunagin M,Raj A,Scholler N,Gross L,Beqiri M,Bennett K,Watson I,Schaider H,Davies MA,Wargo J,Czerniecki BJ,Schuchter L,Herlyn D,Flaherty K,Herlyn M,Wagner SN

Published figure using CD20 monoclonal antibody (Product # 17-0209-42) in Flow Cytometry

Tue Sep 19 00:00:00 EDT 2017

UTX/KDM6A Loss Enhances the Malignant Phenotype of Multiple Myeloma and Sensitizes Cells to EZH2 inhibition.

Cell reports

Ezponda T,Dupéré-Richer D,Will CM,Small EC,Varghese N,Patel T,Nabet B,Popovic R,Oyer J,Bulic M,Zheng Y,Huang X,Shah MY,Maji S,Riva A,Occhionorelli M,Tonon G,Kelleher N,Keats J,Licht JD

17-0209-41 was used in flow cytometry as a surface marker to determine difference in cells +/- UTX expression.

Tue Oct 17 00:00:00 EDT 2017

Creation of an immunodeficient HLA-transgenic mouse (HUMAMICE) and functional validation of human immunity after transfer of HLA-matched human cells.

PloS one

Zeng Y,Liu B,Rubio MT,Wang X,Ojcius DM,Tang R,Durrbach A,Ru Z,Zhou Y,Lone YC

17-0209 was used in Flow cytometry/Cell sorting to generate a novel immuno-deficient mouse strain expressing human HLA molecules instead of mouse MHC molecules.

Tue Sep 05 00:00:00 EDT 2017

Human CD1c(+) DCs are critical cellular mediators of immune responses induced by immunogenic cell death.

Oncoimmunology

Di Blasio S,Wortel IM,van Bladel DA,de Vries LE,Duiveman-de Boer T,Worah K,de Haas N,Buschow SI,de Vries IJ,Figdor CG,Hato SV

17-0209 was used in Flow cytometry/Cell sorting to explore the effect of platinum-induced immunogenic cell death on phenotype and function of human blood circulating dendritic cells.

Mon Aug 01 00:00:00 EDT 2016

Human blood myeloid and plasmacytoid dendritic cells cross activate each other and synergize in inducing NK cell cytotoxicity.

Oncoimmunology

van Beek JJ,Gorris MA,Sköld AE,Hatipoglu I,Van Acker HH,Smits EL,de Vries IJ,Bakdash G

17-0209 was used in Flow cytometry/Cell sorting to indicate that co-application of human blood dendritic cell (DC) subsets could render DC-based anticancer vaccines more efficacious.

Tue Jan 12 00:00:00 EST 2021

Phase I study of a bispecific ligand-directed toxin targeting CD22 and CD19 (DT2219) for refractory B-cell malignancies.

Clinical cancer research : an official journal of the American Association for Cancer Research

Bachanova V,Frankel AE,Cao Q,Lewis D,Grzywacz B,Verneris MR,Ustun C,Lazaryan A,McClune B,Warlick ED,Kantarjian H,Weisdorf DJ,Miller JS,Vallera DA

Published figure using CD20 monoclonal antibody (Product # 17-0209-42) in Flow Cytometry

Sun Mar 15 00:00:00 EDT 2015

Control of Viremia Enables Acquisition of Resting Memory B Cells with Age and Normalization of Activated B Cell Phenotypes in HIV-Infected Children.

Journal of immunology (Baltimore, Md. : 1950)

Muema DM,Macharia GN,Hassan AS,Mwaringa SM,Fegan GW,Berkley JA,Nduati EW,Urban BC

17-0209 was used in Flow cytometry/Cell sorting to investigate the proportion of B cell subsets and frequencies of Ag-specific memory B cells in peripheral blood from HIV-infected children and healthy controls.

Sat Aug 01 00:00:00 EDT 2015

Multispectral imaging reveals the tissue distribution of tetraspanins in human lymphoid organs.

Histochemistry and cell biology

de Winde CM,Zuidscherwoude M,Vasaturo A,van der Schaaf A,Figdor CG,van Spriel AB

17-0209 was used in Flow cytometry/Cell sorting to provide a solid basis for better insight into the function of tetraspanins in the human immune response.

Sat Aug 01 00:00:00 EDT 2015

Monocyte-activation phenotypes are associated with biomarkers of inflammation and coagulation in chronic HIV infection.

The Journal of infectious diseases

Wilson EM,Singh A,Hullsiek KH,Gibson D,Henry WK,Lichtenstein K,Önen NF,Kojic E,Patel P,Brooks JT,Sereti I,Baker JV

17-0209 was used in Flow cytometry/Cell sorting to indicate the association of monocyte-activation phenotypes with biomarkers of inflammation and coagulation in chronic HIV infection.

Sat Nov 01 00:00:00 EDT 2014

Evidence for innate immune system activation in HIV type 1-infected elite controllers.

The Journal of infectious diseases

Krishnan S,Wilson EM,Sheikh V,Rupert A,Mendoza D,Yang J,Lempicki R,Migueles SA,Sereti I

17-0209 was used in Flow cytometry/Cell sorting to study how elite controllers maintain control of plasma HIV viremia and have evidence of an activated innate immune response.

Sat Mar 01 00:00:00 EST 2014

Decreases in colonic and systemic inflammation in chronic HIV infection after IL-7 administration.

PLoS pathogens

Sereti I,Estes JD,Thompson WL,Morcock DR,Fischl MA,Croughs T,Beq S,Lafaye de Micheaux S,Yao MD,Ober A,Wilson EM,Natarajan V,Imamichi H,Boulassel MR,Lederman MM,Routy JP

17-0209 was used in Flow cytometry/Cell sorting to test the efficacy of IL-7 adjunctive therapy on T-cell reconstitution in antiretroviral therapy-suppressed HIV patients.

Wed Jan 01 00:00:00 EST 2014

Analysis of human B-cell responses following ChAd63-MVA MSP1 and AMA1 immunization and controlled malaria infection.

Immunology

Elias SC,Choudhary P,de Cassan SC,Biswas S,Collins KA,Halstead FD,Bliss CM,Ewer KJ,Hodgson SH,Duncan CJ,Hill AV,Draper SJ

17-0209 was used in Flow cytometry/Cell sorting to confirm that mBC and antibody responses can be induced and boosted by blood-stage parasite exposure, in support of epidemiological studies on low-level parasite exposure.

Tue Apr 01 00:00:00 EDT 2014

An efficient low cost method for gene transfer to T lymphocytes.

PloS one

Chicaybam L,Sodre AL,Curzio BA,Bonamino MH

17-0209 was used in Flow cytometry/Cell sorting to describe a novel protocol for electroporation based modification of T lymphocytes.

Mon Sep 30 00:00:00 EDT 2013

Transcriptional profiling of mRNAs and microRNAs in human bone marrow precursor B cells identifies subset- and age-specific variations.

PloS one

Jensen K,Brusletto BS,Aass HC,Olstad OK,Kierulf P,Gautvik KM

Published figure using CD20 monoclonal antibody (Product # 17-0209-42) in Flow Cytometry

Tue Apr 01 00:00:00 EDT 2014

A novel platform to produce human monoclonal antibodies: The next generation of therapeutic human monoclonal antibodies discovery.

mAbs

Duvall M,Bradley N,Fiorini RN

17-0209 was used in Flow cytometry/Cell sorting to study platforms upon which to produce therapeutic human monoclonal antibodies.

Mon Aug 15 00:00:00 EDT 2011

Bioinformatics

Protein Aliases: APY; ATOPY; B-lymphocyte antigen CD20; B-lymphocyte cell-surface antigen B1; B-lymphocyte surface antigen B1; Bp35; CD20; CD20 antigen; CD20 receptor; Fc epsilon receptor I beta chain; Fc Fragment of IgE high affinity I receptor for beta polypeptide; FCER1B; IGEL; IGER; IGHER; LEU16; Leukocyte surface antigen Leu-16; Ly44; Membrane-spanning 4-domains subfamily A member 1; membrane-spanning 4-domains, subfamily A, member 1; MGC3969

View more View less

Gene Aliases: B1; Bp35; CD20; CVID5; LEU-16; MS4A1; MS4A2; S7

View more View less

UniProt ID: (Human) P11836

View more View less

Entrez Gene ID: (Human) 931

View more View less

Function(s)

epidermal growth factor receptor binding protein binding MHC class II protein complex binding transporter

Process(es)

humoral immune response B cell proliferation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

CD20 Monoclonal Antibody (2H7), APC, eBioscience™

CD20 Antibody (17-0209-42) in Flow

Product Details

17-0209-42

Immunohistochemistry (IHC)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!