Invitrogen

CD20 Monoclonal Antibody (2H7), PE-Cyanine7, eBioscience™

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

11 Published Figures

17 References

View all (225) CD20 antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

FIGURE: 1 / 13

CD20 Antibody (25-0209-42) in Flow

Staining of human peripheral blood mononuclear cells with CD45 Pacific Blue, CD19 FITC, CD3 APC, and CD20 PE-Cyanine7. As expected based on known relative expression patterns, CD20 clone 2H7 stains a subset of lymphocytes (pink), but not monocytes (orange) and granulocytes (blue).

Product Image Gallery

CD20 Antibody — Staining of human peripheral blood mononuclear cells with CD45 Pacific Blue, CD19 FITC, CD3 APC, and CD20 PE-Cyanine7. As expected based on known relative expression patterns, CD20 clone 2H7 stains a subset of lymphocytes (pink), but not monocytes (orange) and granulocytes (blue). {RE}

Product Details

25-0209-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.125 µg)/test

View 17 publications 17 publications

Product Specifications

Species Reactivity

Human

Published species

Cynomolgus monkey, Human, Rhesus monkey

Host/Isotype

Mouse / IgG2b, kappa

Recommended Isotype Control

Mouse IgG2b kappa Isotype Control (eBMG2b), PE-Cyanine7, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

2H7

Conjugate

PE-Cyanine7 PE-Cyanine7 PE-Cyanine7

Excitation/Emission Max

569/780 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1834471

Product Specific Information

Description: The 2H7 monoclonal antibody reacts with human CD20, a 33-36 kDa transmembrane protein. CD20 is expressed by developing B cells as well as mature B cells but not plasma cells. CD20 has been detected at low levels on a small subset of mature T cells. It is suggested that CD20 plays a role in B-cell activation.

Applications Reported: This 2H7 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 2H7 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD20 is a non-glycosylated surface phosphoprotein that has a molecular weight range of 33-37 kDa depending on the degree of phosphorylation. CD20 is expressed on mature and most malignant B cells, in a subpopulation of T lymphocytes and follicular dendritic cells. CD20 expression on B cells is synchronous with the expression of surface IgM and it regulates transmembrane calcium conductance, cell cycle progression and B-cell proliferation. CD20 is also associated with lipid rafts, but the intensity of this association depends on extracellular triggering, employing CD20 conformational change, and/or BCR (B cell antigen receptor) aggregation. After the receptor ligation, BCR and CD20 colocalize and then rapidly dissociate before BCR endocytosis, whereas CD20 remains at the cell surface. CD20 serves as a useful target for antibody-mediated therapeutic depletion of B cells, as it is expressed at high levels on most B-cell malignancies, but does not become internalized or shed from the plasma membrane following monoclonal antibody treatment. Diseases associated with CD20 dysfunction include Ms4a1-related common variable immune deficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Spontaneous EBV-Reactivation during B Cell Differentiation as a Model for Polymorphic EBV-Driven Lymphoproliferation.

Cancers

Care MA,Stephenson S,Owen R,Doody GM,Tooze RM

Published figure using CD20 monoclonal antibody (Product # 25-0209-42) in Flow Cytometry

Wed Jun 07 00:00:00 EDT 2023

A Soluble NK-CAR Mediates the Specific Cytotoxicity of NK Cells toward the Target CD20⁺ Lymphoma Cells.

Aging and disease

Liu R,Luo Q,Luo W,Wan L,Zhu Q,Yin X,Lu X,Song Z,Wei L,Xiang Z,Zou Y

Published figure using CD20 monoclonal antibody (Product # 25-0209-42) in Flow Cytometry

Sat Oct 01 00:00:00 EDT 2022

TRPA1 triggers hyperalgesia and inflammation after tooth bleaching.

Scientific reports

Chen C,Huang X,Zhu W,Ding C,Huang P,Li R

Published figure using CD20 monoclonal antibody (Product # 25-0209-42) in Flow Cytometry

Tue Aug 31 00:00:00 EDT 2021

Transcriptome Profiling Reveals B-Lineage Cells Contribute to the Poor Prognosis and Metastasis of Clear Cell Renal Cell Carcinoma.

Frontiers in oncology

Yang F,Zhao J,Luo X,Li T,Wang Z,Wei Q,Lu H,Meng Y,Cai K,Lu L,Lu Y,Chen L,Sooranna SR,Luo L,Song J,Meng L

25-0209-42 was used in Flow cytometry/Cell sorting to explore the pathological roles of B-lineage cells in clear cell renal cell carcinoma (ccRCC) and investigate whether targeting the pro-metastatic immune cell in the ccRCC microenvironment could reduce the proportion of ccRCC patients who continue to progress to metastasis after immune therapy.

Tue Sep 07 00:00:00 EDT 2021

Early Transcriptional Changes within Liver, Adrenal Gland, and Lymphoid Tissues Significantly Contribute to Ebola Virus Pathogenesis in Cynomolgus Macaques.

Journal of virology

Jankeel A,Menicucci AR,Woolsey C,Fenton KA,Mendoza N,Versteeg K,Cross RW,Geisbert TW,Messaoudi I

25-0209 was used in Flow cytometry/Cell sorting to track longitudinal transcriptional changes in tissues collected from EBOV-Makona-infected cynomolgus macaques.

Mon May 18 00:00:00 EDT 2020

Relative safety of various spermatogenic stem cell purification methods for application in spermatogenic stem cell transplantation.

Stem cell research & therapy

Tian J,Ma K,Pei CB,Zhang SH,Li X,Zhou Y,Yan B,Wang HY,Ma LH

Published figure using CD20 monoclonal antibody (Product # 25-0209-42) in Flow Cytometry

Mon Dec 16 00:00:00 EST 2019

Rapid Germinal Center and Antibody Responses in Non-human Primates after a Single Nanoparticle Vaccine Immunization.

Cell reports

Havenar-Daughton C,Carnathan DG,Boopathy AV,Upadhyay AA,Murrell B,Reiss SM,Enemuo CA,Gebru EH,Choe Y,Dhadvai P,Viviano F,Kaushik K,Bhiman JN,Briney B,Burton DR,Bosinger SE,Schief WR,Irvine DJ,Silvestri G,Crotty S

Published figure using CD20 monoclonal antibody (Product # 25-0209-42) in Flow Cytometry

Tue Nov 12 00:00:00 EST 2019

Genetic modification of primary human B cells to model high-grade lymphoma.

Nature communications

Caeser R,Di Re M,Krupka JA,Gao J,Lara-Chica M,Dias JML,Cooke SL,Fenner R,Usheva Z,Runge HFP,Beer PA,Eldaly H,Pak HK,Park CS,Vassiliou GS,Huntly BJP,Mupo A,Bashford-Rogers RJM,Hodson DJ

Published figure using CD20 monoclonal antibody (Product # 25-0209-42) in Flow Cytometry

Fri Oct 04 00:00:00 EDT 2019

Evidence for persistence of the SHIV reservoir early after MHC haploidentical hematopoietic stem cell transplantation.

Nature communications

Colonna L,Peterson CW,Schell JB,Carlson JM,Tkachev V,Brown M,Yu A,Reddy S,Obenza WM,Nelson V,Polacino PS,Mack H,Hu SL,Zeleski K,Hoffman M,Olvera J,Furlan SN,Zheng H,Taraseviciute A,Hunt DJ,Betz K,Lane JF,Vogel K,Hotchkiss CE,Moats C,Baldessari A,Murnane RD,English C,Astley CA,Wangari S,Agricola B,Ahrens J,Iwayama N,May A,Stensland L,Huang MW,Jerome KR,Kiem HP,Kean LS

Published figure using CD20 monoclonal antibody (Product # 25-0209-42) in Flow Cytometry

Thu Oct 25 00:00:00 EDT 2018

Tumor-associated B-cells induce tumor heterogeneity and therapy resistance.

Nature communications

Somasundaram R,Zhang G,Fukunaga-Kalabis M,Perego M,Krepler C,Xu X,Wagner C,Hristova D,Zhang J,Tian T,Wei Z,Liu Q,Garg K,Griss J,Hards R,Maurer M,Hafner C,Mayerhöfer M,Karanikas G,Jalili A,Bauer-Pohl V,Weihsengruber F,Rappersberger K,Koller J,Lang R,Hudgens C,Chen G,Tetzlaff M,Wu L,Frederick DT,Scolyer RA,Long GV,Damle M,Ellingsworth C,Grinman L,Choi H,Gavin BJ,Dunagin M,Raj A,Scholler N,Gross L,Beqiri M,Bennett K,Watson I,Schaider H,Davies MA,Wargo J,Czerniecki BJ,Schuchter L,Herlyn D,Flaherty K,Herlyn M,Wagner SN

Published figure using CD20 monoclonal antibody (Product # 25-0209-42) in Flow Cytometry

Tue Sep 19 00:00:00 EDT 2017

CD16⁺ Monocyte Subset Was Enriched and Functionally Exacerbated in Driving T-Cell Activation and B-Cell Response in Systemic Lupus Erythematosus.

Frontiers in immunology

Zhu H,Hu F,Sun X,Zhang X,Zhu L,Liu X,Li X,Xu L,Shi L,Gan Y,Su Y

25-0209 was used in Flow cytometry/Cell sorting to demonstrate that CD16+monocytes were enriched and played a critical role in driving the pathogenic T- and B-cell responses in patients with systemic lupus erythematosus.

Wed Nov 20 00:00:00 EST 2019

Sialylation of IgG Fc domain impairs complement-dependent cytotoxicity.

The Journal of clinical investigation

Quast I,Keller CW,Maurer MA,Giddens JP,Tackenberg B,Wang LX,Münz C,Nimmerjahn F,Dalakas MC,Lünemann JD

25-0209 was used in Flow cytometry/Cell sorting to investigate the effect of IgG sialylation on human monoclonal IgG1 molecules.

Mon Nov 02 00:00:00 EST 2015

Phase I study of a bispecific ligand-directed toxin targeting CD22 and CD19 (DT2219) for refractory B-cell malignancies.

Clinical cancer research : an official journal of the American Association for Cancer Research

Bachanova V,Frankel AE,Cao Q,Lewis D,Grzywacz B,Verneris MR,Ustun C,Lazaryan A,McClune B,Warlick ED,Kantarjian H,Weisdorf DJ,Miller JS,Vallera DA

Published figure using CD20 monoclonal antibody (Product # 25-0209-42) in Flow Cytometry

Sun Mar 15 00:00:00 EDT 2015

Characterization of simian immunodeficiency virus (SIV) that induces SIV encephalitis in rhesus macaques with high frequency: role of TRIM5 and major histocompatibility complex genotypes and early entry to the brain.

Journal of virology

Matsuda K,Dang Q,Brown CR,Keele BF,Wu F,Ourmanov I,Goeken R,Whitted S,Riddick NE,Buckler-White A,Hirsch VM

25-0209 was used in Flow cytometry/Cell sorting to describe the development of a novel SIV isolate that induces neurologic disease in a high proportion of rhesus macaques.

Sat Nov 01 00:00:00 EDT 2014

An efficient low cost method for gene transfer to T lymphocytes.

PloS one

Chicaybam L,Sodre AL,Curzio BA,Bonamino MH

Published figure using CD20 monoclonal antibody (Product # 25-0209-42) in Flow Cytometry

Mon Sep 30 00:00:00 EDT 2013

Transcriptional profiling of mRNAs and microRNAs in human bone marrow precursor B cells identifies subset- and age-specific variations.

PloS one

Jensen K,Brusletto BS,Aass HC,Olstad OK,Kierulf P,Gautvik KM

Published figure using CD20 monoclonal antibody (Product # 25-0209-42) in Flow Cytometry

Tue Apr 01 00:00:00 EDT 2014

Development of neurological disease is associated with increased immune activation in simian immunodeficiency virus-infected macaques.

Journal of virology

Dang Q,Whitted S,Goeken RM,Brenchley JM,Matsuda K,Brown CR,Lafont BA,Starost MF,Iyengar R,Plishka RJ,Buckler-White A,Hirsch VM

25-0209 was used in Flow cytometry/Cell sorting to demonstrate that elevated levels of systemic immune activation correlate with viral RNA in the cerebral spinal fluid but not with plasma viral load.

Sat Dec 01 00:00:00 EST 2012

Bioinformatics

Protein Aliases: APY; ATOPY; B-lymphocyte antigen CD20; B-lymphocyte cell-surface antigen B1; B-lymphocyte surface antigen B1; Bp35; CD20; CD20 antigen; CD20 receptor; Fc epsilon receptor I beta chain; Fc Fragment of IgE high affinity I receptor for beta polypeptide; FCER1B; IGEL; IGER; IGHER; LEU16; Leukocyte surface antigen Leu-16; Ly44; Membrane-spanning 4-domains subfamily A member 1; membrane-spanning 4-domains, subfamily A, member 1; MGC3969

View more View less

Gene Aliases: B1; Bp35; CD20; CVID5; LEU-16; MS4A1; MS4A2; S7

View more View less

UniProt ID: (Human) P11836

View more View less

Entrez Gene ID: (Human) 931

View more View less

Function(s)

epidermal growth factor receptor binding protein binding MHC class II protein complex binding transporter

Process(es)

humoral immune response B cell proliferation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

CD20 Monoclonal Antibody (2H7), PE-Cyanine7, eBioscience™

CD20 Antibody (25-0209-42) in Flow

Product Details

25-0209-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!