Invitrogen

CD4 Monoclonal Antibody (RPA-T4), eBioscience™

FIGURE: 1 / 57

CD4 Antibody (14-0049-82) in Flow

Staining of normal human peripheral blood cells with 0.5 µg of Mouse IgG1 kappa Isotype Control Purified (Product # 14-4714-82) (open histogram) or 0.5 µg of Anti-Human CD4 Purified (filled histogram) followed by Anti-Mouse IgG FITC (Product # 11-4011-85). Cells in the lymphocyte gate were used for analysis.

CD4 Antibody in Immunocytochemistry (ICC/IF)

CD4 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

Product Details

14-0049-82

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

Assay-Dependent

View 6 publications 6 publications

Immunocytochemistry (ICC/IF)

View 4 publications 4 publications

Flow Cytometry (Flow)

1 µg/test

View 54 publications 54 publications

Neutralization (Neu)

Assay-Dependent

View 3 publications 3 publications

Functional Assay (Functional)

Assay-Dependent

View 3 publications 3 publications

Inhibition Assays (IA)

View 1 publication 1 publication

Fluorescence Resonance Energy Transfer (FRET)

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

RPA-T4

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_467077

Product Specific Information

Description: The RPA-T4 monoclonal antibody reacts with human CD4, a 59 kDa cell surface receptor expressed by a majority of thymocytes, subpopulation of mature T cells (T-helper cells) and in low levels on monocytes. CD4 is a receptor for the human immunodeficiency virus (HIV). RPA-T4 blocks HIV binding and mixed lymphocyte reaction. The RPA-T4 antibody recognizes a different epitope than the OKT4 monoclonal antibody, and these antibodies do not cross-block binding to each other's respective epitopes.

Applications Reported: The RPA-T4 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining. RPA-T4 has also been reported for use in blocking of CD4 activity in in vitro functional assays (Please use Functional Grade purified RPA-T4, Product # 16-0049, in functional assays).

Applications Tested: The RPA-T4 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The CD4 antigen is involved in the recognition of MHC class II molecules and is a co-receptor for HIV. CD4 is primarily expressed in a subset of T-lymphocytes, also referred to as T helper cells, but may also be expressed by other cells in the immune system, such as monocytes, macrophages, and dendritic cells. At the tissue level, CD4 expression may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues. CD4 functions to initiate or augment the early phase of T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase, Lck. It may also function as an important mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcripts have been identified in this gene [RefSeq, July 2017].

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Cathepsin S inhibition in dendritic cells prevents Th17 cell differentiation in perivascular adipose tissues following vascular injury in diabetic rats.

Journal of biochemical and molecular toxicology

Peng H,Lv Y,Li C,Cheng Z,He S,Wang C,Liu J

Published figure using CD4 monoclonal antibody (Product # 14-0049-82) in Flow Cytometry

Fri Sep 01 00:00:00 EDT 2023

Rapamycin improves Graves' orbitopathy by suppressing CD4+ cytotoxic T lymphocytes.

JCI insight

Zhang M,Chong KK,Chen ZY,Guo H,Liu YF,Kang YY,Li YJ,Shi TT,Lai KK,He MQ,Ye K,Kahaly GJ,Shi BY,Wang Y

Published figure using CD4 monoclonal antibody (Product # 14-0049-82) in Flow Cytometry

Wed Feb 08 00:00:00 EST 2023

Increased levels of endogenous retroviruses trigger fibroinflammation and play a role in kidney disease development.

Nature communications

Dhillon P,Mulholland KA,Hu H,Park J,Sheng X,Abedini A,Liu H,Vassalotti A,Wu J,Susztak K

14-0049-82 was used in Immunohistochemistry-immunofluorescence to indicate an important role of epigenetic derepression-induced ERV activation triggering renal fibroinflammation.

Thu Feb 02 00:00:00 EST 2023

Induced Human Regulatory T Cells Express the Glucagon-like Peptide-1 Receptor.

Cells

Rode AKO,Buus TB,Mraz V,Al-Jaberi FAH,Lopez DV,Ford SL,Hennen S,Eliasen IP,Klewe IV,Gharehdaghi L,Dragan A,Rosenkilde MM,Woetmann A,Skov L,Ødum N,Bonefeld CM,Kongsbak-Wismann M,Geisler C

Published figure using CD4 monoclonal antibody (Product # 14-0049-82) in Immunohistochemistry

Fri Aug 19 00:00:00 EDT 2022

Single-cell RNA sequencing reveals the molecular features of peripheral blood immune cells in children, adults and centenarians.

Frontiers in immunology

Zhong J,Ding R,Jiang H,Li L,Wan J,Feng X,Chen M,Peng L,Li X,Lin J,Yang H,Wang M,Li Q,Chen Q

Published figure using CD4 monoclonal antibody (Product # 14-0049-82) in Flow Cytometry

Mon Jan 30 00:00:00 EST 2023

Single-cell profiling of healthy human kidney reveals features of sex-based transcriptional programs and tissue-specific immunity.

Nature communications

McEvoy CM,Murphy JM,Zhang L,Clotet-Freixas S,Mathews JA,An J,Karimzadeh M,Pouyabahar D,Su S,Zaslaver O,Röst H,Arambewela R,Liu LY,Zhang S,Lawson KA,Finelli A,Wang B,MacParland SA,Bader GD,Konvalinka A,Crome SQ

Published figure using CD4 monoclonal antibody (Product # 14-0049-82) in Flow Cytometry

Sat Dec 10 00:00:00 EST 2022

Decoding the pathogenesis of Diamond-Blackfan anemia using single-cell RNA-seq.

Cell discovery

Wang B,Wang C,Wan Y,Gao J,Ma Y,Zhang Y,Tong J,Zhang Y,Liu J,Chang L,Xu C,Shen B,Chen Y,Jiang E,Kurita R,Nakamura Y,Lim KC,Engel JD,Zhou J,Cheng T,Zhu X,Zhu P,Shi L

Published figure using CD4 monoclonal antibody (Product # 14-0049-82) in Flow Cytometry

Tue May 10 00:00:00 EDT 2022

Effects of the Antidiabetic Drugs Evogliptin and Sitagliptin on the Immune Function of CD26/DPP4 in Th1 Cells.

Biomolecules & therapeutics

Yoon H,Sung JH,Song MJ

Published figure using CD4 monoclonal antibody (Product # 14-0049-82) in Flow Cytometry

Mon Mar 01 00:00:00 EST 2021

Murlentamab, a Low Fucosylated Anti-Müllerian Hormone Type II Receptor (AMHRII) Antibody, Exhibits Anti-Tumor Activity through Tumor-Associated Macrophage Reprogrammation and T Cell Activation.

Cancers

Prat M,Salon M,Allain T,Dubreuil O,Noël G,Preisser L,Jean B,Cassard L,Lemée F,Tabah-Fish I,Pipy B,Jeannin P,Prost JF,Barret JM,Coste A

Published figure using CD4 monoclonal antibody (Product # 14-0049-82) in Flow Cytometry

Tue Apr 13 00:00:00 EDT 2021

Characterization and modulation of anti-αβTCR antibodies and their respective binding sites at the βTCR chain to enrich engineered T cells.

Molecular therapy. Methods & clinical development

Kierkels GJJ,van Diest E,Hernández-López P,Scheper W,de Bruin ACM,Frijlink E,Aarts-Riemens T,van Dooremalen SFJ,Beringer DX,Oostvogels R,Kramer L,Straetemans T,Uckert W,Sebestyén Z,Kuball J

Published figure using CD4 monoclonal antibody (Product # 14-0049-82) in Flow Cytometry

Fri Sep 10 00:00:00 EDT 2021

Bispecific T-Cell Engaging Antibodies Against MUC16 Demonstrate Efficacy Against Ovarian Cancer in Monotherapy and in Combination With PD-1 and VEGF Inhibition.

Frontiers in immunology

Yeku OO,Rao TD,Laster I,Kononenko A,Purdon TJ,Wang P,Cui Z,Liu H,Brentjens RJ,Spriggs D

Published figure using CD4 monoclonal antibody (Product # 14-0049-82) in Flow Cytometry

Thu Oct 21 00:00:00 EDT 2021

Identification of two HLA-A*0201 immunogenic epitopes of lactate dehydrogenase C (LDHC): potential novel targets for cancer immunotherapy.

Cancer immunology, immunotherapy : CII

Thomas R,Shaath H,Naik A,Toor SM,Elkord E,Decock J

Published figure using CD4 monoclonal antibody (Product # 14-0049-82) in Flow Cytometry

Sun Mar 01 00:00:00 EST 2020

Activation of Human Vδ2⁺ γδ T Cells by Staphylococcus aureus Promotes Enhanced Anti-Staphylococcal Adaptive Immunity.

Journal of immunology (Baltimore, Md. : 1950)

Cooper AJR,Lalor SJ,McLoughlin RM

14-0049-82 was used in Flow Cytometry to study the capacity of human Vδ2+ γδ T cells for rapid activation in response to S. aureus.

Sat Aug 15 00:00:00 EDT 2020

HDAC Inhibitor, CG-745, Enhances the Anti-Cancer Effect of Anti-PD-1 Immune Checkpoint Inhibitor by Modulation of the Immune Microenvironment.

Journal of Cancer

Kim YD,Park SM,Ha HC,Lee AR,Won H,Cha H,Cho S,Cho JM

Published figure using CD4 monoclonal antibody (Product # 14-0049-82) in Flow Cytometry

Mon Sep 28 00:00:00 EDT 2020

Involvement of natural killer cells in the pathogenesis of endometriosis in patients with pelvic pain.

The Journal of international medical research

He J,Xu Y,Yi M,Gu C,Zhu Y,Hu G

Published figure using CD4 monoclonal antibody (Product # 14-0049-82) in Flow Cytometry

Wed Jul 01 00:00:00 EDT 2020

Cytoplasmic Citrate Flux Modulates the Immune Stimulatory NKG2D Ligand MICA in Cancer Cells.

Frontiers in immunology

Møller SH,Mellergaard M,Madsen M,Bermejo AV,Jepsen SD,Hansen MH,Høgh RI,Aldana BI,Desler C,Rasmussen LJ,Sustarsic EG,Gerhart-Hines Z,Daskalaki E,Wheelock CE,Hiron TK,Lin D,O'Callaghan CA,Wandall HH,Andresen L,Skov S

Published figure using CD4 monoclonal antibody (Product # 14-0049-82) in Neutralization

Fri Apr 30 00:00:00 EDT 2021

Standardized 11-color flow cytometry panel for the functional phenotyping of human T regulatory cells.

Journal of biological methods

Manuszak C,Brainard M,Thrash E,Hodi FS,Severgnini M

Published figure using CD4 monoclonal antibody (Product # 14-0049-82) in Flow Cytometry

Mon Sep 28 00:00:00 EDT 2020

Phase I Study of Ficlatuzumab and Cetuximab in Cetuximab-Resistant, Recurrent/Metastatic Head and Neck Cancer.

Cancers

Bauman JE,Ohr J,Gooding WE,Ferris RL,Duvvuri U,Kim S,Johnson JT,Soloff AC,Wallweber G,Winslow J,Gaither-Davis A,Grandis JR,Stabile LP

14-0049-82 was used in Flow Cytometry to establish the recommended Phase II dose (RP2D). Secondary objectives included overall response rate (ORR), progression-free survival (PFS), and overall survival (OS).

Thu Jun 11 00:00:00 EDT 2020

MicroRNA‑155 inhibits the proliferation of CD8+ T cells via upregulating regulatory T cells in vitiligo.

Molecular medicine reports

Lv M,Li Z,Liu J,Lin F,Zhang Q,Li Z,Wang Y,Wang K,Xu Y

Published figure using CD4 monoclonal antibody (Product # 14-0049-82) in Flow Cytometry

Tue Oct 01 00:00:00 EDT 2019

RAB11FIP5 Expression and Altered Natural Killer Cell Function Are Associated with Induction of HIV Broadly Neutralizing Antibody Responses.

Cell

Bradley T,Peppa D,Pedroza-Pacheco I,Li D,Cain DW,Henao R,Venkat V,Hora B,Chen Y,Vandergrift NA,Overman RG,Edwards RW,Woods CW,Tomaras GD,Ferrari G,Ginsburg GS,Connors M,Cohen MS,Moody MA,Borrow P,Haynes BF

Published figure using CD4 monoclonal antibody (Product # 14-0049-82) in Flow Cytometry

Thu Oct 04 00:00:00 EDT 2018

The Effects of High Mobility Group Box-1 Protein on Peripheral Treg/Th17 Balance in Patients with Atherosclerosis.

Acta Cardiologica Sinica

Ding JW,Zhou T,Zheng XX,Wang XA,Tong XH,Luo CY,Zhang ZQ,Yu B

Published figure using CD4 monoclonal antibody (Product # 14-0049-82) in Flow Cytometry

Sat Sep 01 00:00:00 EDT 2018

Non-neutralizing Antibodies Alter the Course of HIV-1 Infection In Vivo.

Cell

Horwitz JA,Bar-On Y,Lu CL,Fera D,Lockhart AAK,Lorenzi JCC,Nogueira L,Golijanin J,Scheid JF,Seaman MS,Gazumyan A,Zolla-Pazner S,Nussenzweig MC

Published figure using CD4 monoclonal antibody (Product # 14-0049-82) in Flow Cytometry

Thu Aug 10 00:00:00 EDT 2017

Blocking the recruitment of naive CD4⁺ T cells reverses immunosuppression in breast cancer.

Cell research

Su S,Liao J,Liu J,Huang D,He C,Chen F,Yang L,Wu W,Chen J,Lin L,Zeng Y,Ouyang N,Cui X,Yao H,Su F,Huang JD,Lieberman J,Liu Q,Song E

Published figure using CD4 monoclonal antibody (Product # 14-0049-82) in Flow Cytometry

Sat Apr 01 00:00:00 EDT 2017

Impact of Enhancer of Zeste Homolog 2 on T Helper Cell-Mediated Allergic Rhinitis.

Frontiers in immunology

Hou TY,Chen MR,Chou YC,Kan PC,Tsai YT,Cha TL

Published figure using CD4 monoclonal antibody (Product # 14-0049-82) in Flow Cytometry

Wed Nov 20 00:00:00 EST 2019

Protein kinase CK2 controls T-cell polarization through dendritic cell activation in response to contact sensitizers.

Journal of leukocyte biology

de Bourayne M,Gallais Y,El Ali Z,Rousseau P,Damiens MH,Cochet C,Filhol O,Chollet-Martin S,Pallardy M,Kerdine-Römer S

Published figure using CD4 monoclonal antibody (Product # 14-0049-82) in Flow Cytometry

Wed Mar 01 00:00:00 EST 2017

CXCL13-producing TFH cells link immune suppression and adaptive memory in human breast cancer.

JCI insight

Gu-Trantien C,Migliori E,Buisseret L,de Wind A,Brohée S,Garaud S,Noël G,Dang Chi VL,Lodewyckx JN,Naveaux C,Duvillier H,Goriely S,Larsimont D,Willard-Gallo K

Published figure using CD4 monoclonal antibody (Product # 14-0049-82) in Flow Cytometry

Fri Jun 02 00:00:00 EDT 2017

Human in vitro-induced regulatory T cells display Dlgh1dependent and PKC-θ restrained suppressive activity.

Scientific reports

Zanin-Zhorov A,Kumari S,Hippen KL,Merkel SC,MacMillan ML,Blazar BR,Dustin ML

14-0049 was used in Flow cytometry/Cell sorting to study the regulatory mechanisms behind induced human regulatory T cells (iTregs) in vitro.

Mon Jun 26 00:00:00 EDT 2017

An asthma-associated IL4R variant exacerbates airway inflammation by promoting conversion of regulatory T cells to TH17-like cells.

Nature medicine

Massoud AH,Charbonnier LM,Lopez D,Pellegrini M,Phipatanakul W,Chatila TA

14-0049 was used in Flow cytometry/Cell sorting to identify a previously unknown mechanism for the development of TH17 cell inflammation in genetically prone individuals.

Thu Sep 01 00:00:00 EDT 2016

Remarkably low affinity of CD4/peptide-major histocompatibility complex class II protein interactions.

Proceedings of the National Academy of Sciences of the United States of America

Jönsson P,Southcombe JH,Santos AM,Huo J,Fernandes RA,McColl J,Lever M,Evans EJ,Hudson A,Chang VT,Hanke T,Godkin A,Dunne PD,Horrocks MH,Palayret M,Screaton GR,Petersen J,Rossjohn J,Fugger L,Dushek O,Xu XN,Davis SJ,Klenerman D

Published figure using CD4 monoclonal antibody (Product # 14-0049-82) in Flow Cytometry

Tue May 17 00:00:00 EDT 2016

Remarkably low affinity of CD4/peptide-major histocompatibility complex class II protein interactions.

Proceedings of the National Academy of Sciences of the United States of America

Published figure using CD4 monoclonal antibody (Product # 14-0049-82) in Flow Cytometry

Tue May 17 00:00:00 EDT 2016

Interaction among activated lymphocytes and mesenchymal cells through podoplanin is critical for a high IL-17 secretion.

Arthritis research & therapy

Noack M,Ndongo-Thiam N,Miossec P

Published figure using CD4 monoclonal antibody (Product # 14-0049-82) in Flow Cytometry

Thu Jun 23 00:00:00 EDT 2016

CD28 family of receptors on T cells in chronic HBV infection: Expression characteristics, clinical significance and correlations with PD-1 blockade.

Molecular medicine reports

Tang ZS,Hao YH,Zhang EJ,Xu CL,Zhou Y,Zheng X,Yang DL

Published figure using CD4 monoclonal antibody (Product # 14-0049-82) in Flow Cytometry

Mon Aug 01 00:00:00 EDT 2016

Equilibrium of Treg/Th17 cells of peripheral blood in syphilitic patients with sero-resistance.

Experimental and therapeutic medicine

Zhao J,Ma J,Zhang X,Li Q,Yang X

Published figure using CD4 monoclonal antibody (Product # 14-0049-82) in Flow Cytometry

Wed Jun 01 00:00:00 EDT 2016

Tuning of AKT-pathway by Nef and its blockade by protease inhibitors results in limited recovery in latently HIV infected T-cell line.

Scientific reports

Kumar A,Abbas W,Colin L,Khan KA,Bouchat S,Varin A,Larbi A,Gatot JS,Kabeya K,Vanhulle C,Delacourt N,Pasquereau S,Coquard L,Borch A,König R,Clumeck N,De Wit S,Rohr O,Rouzioux C,Fulop T,Van Lint C,Herbein G

Published figure using CD4 monoclonal antibody (Product # 14-0049-82) in Flow Cytometry

Thu Apr 14 00:00:00 EDT 2016

Lack of T Cell Response to iPSC-Derived Retinal Pigment Epithelial Cells from HLA Homozygous Donors.

Stem cell reports

Sugita S,Iwasaki Y,Makabe K,Kimura T,Futagami T,Suegami S,Takahashi M

Published figure using CD4 monoclonal antibody (Product # 14-0049-82) in Flow Cytometry

Tue Oct 11 00:00:00 EDT 2016

BCL6 orchestrates Tfh cell differentiation via multiple distinct mechanisms.

The Journal of experimental medicine

Hatzi K,Nance JP,Kroenke MA,Bothwell M,Haddad EK,Melnick A,Crotty S

14-0049 was used in Flow cytometry/Cell sorting to demonstrate the effects of BCL6 on Tfh biology and provide insight into how it mediates distinct cell context-dependent phenotypes.

Mon Apr 06 00:00:00 EDT 2015

Brief Report: IFIH1 Mutation Causes Systemic Lupus Erythematosus With Selective IgA Deficiency.

Arthritis & rheumatology (Hoboken, N.J.)

Van Eyck L,De Somer L,Pombal D,Bornschein S,Frans G,Humblet-Baron S,Moens L,de Zegher F,Bossuyt X,Wouters C,Liston A

14-0049 was used in Flow cytometry/Cell sorting to determine the underlying genetic defect responsible for severe early onset and refractory systemic lupus erythematosus.

Mon Jun 01 00:00:00 EDT 2015

The HIV-1 envelope protein gp120 is captured and displayed for B cell recognition by SIGN-R1(+) lymph node macrophages.

eLife

Park C,Arthos J,Cicala C,Kehrl JH

14-0049 was used in Immunohistochemistry to reveal a specialised lymph node antigen delivery system capable of delivering gp120 and other pathogen-derived glycoproteins to B cells.

Mon Aug 10 00:00:00 EDT 2015

Thymic HIV-2 infection uncovers posttranscriptional control of viral replication in human thymocytes.

Journal of virology

Nunes-Cabaço H,Matoso P,Foxall RB,Tendeiro R,Pires AR,Carvalho T,Pinheiro AI,Soares RS,Sousa AE

14-0049 was used in Flow cytometry/Cell sorting to assess the direct impact of HIV-2 infection on the human thymus.

Sun Feb 01 00:00:00 EST 2015

CCR5 susceptibility to ligand-mediated down-modulation differs between human T lymphocytes and myeloid cells.

Journal of leukocyte biology

Fox JM,Kasprowicz R,Hartley O,Signoret N

14-0049 was used in Functional assays to compare the behavior of CCR5 on in vitro generated human T-cell blasts, monocytes and MDMs and CHO-CCR5 transfectants.

Wed Jul 01 00:00:00 EDT 2015

Liposomal Glutathione Supplementation Restores TH1 Cytokine Response to Mycobacterium tuberculosis Infection in HIV-Infected Individuals.

Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research

Ly J,Lagman M,Saing T,Singh MK,Tudela EV,Morris D,Anderson J,Daliva J,Ochoa C,Patel N,Pearce D,Venketaraman V

Published figure using CD4 monoclonal antibody (Product # 14-0049-82) in Flow Cytometry

Sun Nov 01 00:00:00 EDT 2015

Distinct patterns of intratumoral immune cell infiltrates in patients with HPV-associated compared to non-virally induced head and neck squamous cell carcinoma.

Oncoimmunology

Partlová S,Bouček J,Kloudová K,Lukešová E,Zábrodský M,Grega M,Fučíková J,Truxová I,Tachezy R,Špíšek R,Fialová A

Published figure using CD4 monoclonal antibody (Product # 14-0049-82) in Flow Cytometry

Thu Jan 01 00:00:00 EST 2015

dNP2 is a blood-brain barrier-permeable peptide enabling ctCTLA-4 protein delivery to ameliorate experimental autoimmune encephalomyelitis.

Nature communications

Lim S,Kim WJ,Kim YH,Lee S,Koo JH,Lee JA,Yoon H,Kim DH,Park HJ,Kim HM,Lee HG,Yun Kim J,Lee JU,Hun Shin J,Kyun Kim L,Doh J,Kim H,Lee SK,Bothwell ALM,Suh M,Choi JM

Published figure using CD4 monoclonal antibody (Product # 14-0049-82) in Flow Cytometry

Tue Sep 15 00:00:00 EDT 2015

Investigating the causes for decreased levels of glutathione in individuals with type II diabetes.

PloS one

Lagman M,Ly J,Saing T,Kaur Singh M,Vera Tudela E,Morris D,Chi PT,Ochoa C,Sathananthan A,Venketaraman V

Published figure using CD4 monoclonal antibody (Product # 14-0049-82) in Flow Cytometry

Wed Dec 30 00:00:00 EST 2015

Generation of a cord blood-derived Wilms Tumor 1 dendritic cell vaccine for AML patients treated with allogeneic cord blood transplantation.

Oncoimmunology

de Haar C,Plantinga M,Blokland NJ,van Til NP,Flinsenberg TW,Van Tendeloo VF,Smits EL,Boon L,Spel L,Boes M,Boelens JJ,Nierkens S

14-0049 was used in Flow cytometry/Cell sorting to investigate the generation of a cord-blood derived Wilms Tumor 1 dendritic cell vaccine for cord blood transplanted AML patients.

Sun Nov 01 00:00:00 EDT 2015

CD4+ and CD8+ T cells have opposing roles in breast cancer progression and outcome.

Oncotarget

Huang Y,Ma C,Zhang Q,Ye J,Wang F,Zhang Y,Hunborg P,Varvares MA,Hoft DF,Hsueh EC,Peng G

14-0049 was used in Immunohistochemistry to delineate the dynamics and roles of CD4+ and CD8+ T cells in the pathogenesis of breast cancer.

Fri Jul 10 00:00:00 EDT 2015

The HDAC6/APOBEC3G complex regulates HIV-1 infectiveness by inducing Vif autophagic degradation.

Retrovirology

Valera MS,de Armas-Rillo L,Barroso-González J,Ziglio S,Batisse J,Dubois N,Marrero-Hernández S,Borel S,García-Expósito L,Biard-Piechaczyk M,Paillart JC,Valenzuela-Fernández A

14-0049 was used in Neutralization experiments to identify that the cellular complex, HDAC6/A3G, may be a antiviral factor capable of controlling HIV-1 infectiveness via degradation of Vif.

Wed Jun 24 00:00:00 EDT 2015

Immune response to JC virus T antigen in patients with and without colorectal neoplasia.

Gut microbes

Butcher LD,Garcia M,Arnold M,Ueno H,Goel A,Boland CR

Published figure using CD4 monoclonal antibody (Product # 14-0049-82) in Flow Cytometry

Tue Jul 01 00:00:00 EDT 2014

CD4 ligation on human blood monocytes triggers macrophage differentiation and enhances HIV infection.

Journal of virology

Zhen A,Krutzik SR,Levin BR,Kasparian S,Zack JA,Kitchen SG

14-0049 was used in Flow cytometry/Cell sorting to investigate the role of the CD4 molecule in human monocyte development and function, showing that CD4 ligation triggers macrophage differentiation and enhances HIV infection.

Mon Sep 01 00:00:00 EDT 2014

CD4 ligation on human blood monocytes triggers macrophage differentiation and enhances HIV infection.

Journal of virology

Zhen A,Krutzik SR,Levin BR,Kasparian S,Zack JA,Kitchen SG

Mon Sep 01 00:00:00 EDT 2014

TLR4 activation enhances the PD-L1-mediated tolerogenic capacity of colonic CD90+ stromal cells.

Journal of immunology (Baltimore, Md. : 1950)

Beswick EJ,Johnson JR,Saada JI,Humen M,House J,Dann S,Qiu S,Brasier AR,Powell DW,Reyes VE,Pinchuk IV

14-0049 was used in Flow cytometry/Cell sorting to investigate whether signalling through TLRs contribute to the regulation PD-1 ligands on CD90(+) myofibroblasts/fibroblasts upon colonic mucosal tolerance.

Mon Sep 01 00:00:00 EDT 2014

Akt-dependent enhanced migratory capacity of Th17 cells from children with lupus nephritis.

Journal of immunology (Baltimore, Md. : 1950)

Kshirsagar S,Riedl M,Billing H,Tönshoff B,Thangavadivel S,Steuber C,Staude H,Wechselberger G,Edelbauer M

14-0049 was used in Flow cytometry/Cell sorting to determine that therapeutic modulation of Akt activity could suppress chronic inflammatory processes in children with lupus.

Sat Nov 15 00:00:00 EST 2014

OMIP-022: Comprehensive assessment of antigen-specific human T-cell functionality and memory.

Cytometry. Part A : the journal of the International Society for Analytical Cytology

Graves AJ,Padilla MG,Hokey DA

Published figure using CD4 monoclonal antibody (Product # 14-0049-82) in Flow Cytometry

Tue Jul 01 00:00:00 EDT 2014

Cannabinoid receptor 2 as a potential therapeutic target in rheumatoid arthritis.

BMC musculoskeletal disorders

Fukuda S,Kohsaka H,Takayasu A,Yokoyama W,Miyabe C,Miyabe Y,Harigai M,Miyasaka N,Nanki T

14-0049 was used in Immunofluorescence to suggest that a selective CB(2) agonist could be a new therapy for rheumatoid arthritis that inhibits production of inflammatory mediators from FLS, and osteoclastogenesis.

Tue Aug 12 00:00:00 EDT 2014

High avidity CD8+ T cells efficiently eliminate motile HIV-infected targets and execute a locally focused program of anti-viral function.

PloS one

Foley MH,Forcier T,McAndrew E,Gonzalez M,Chen H,Juelg B,Walker BD,Irvine DJ

14-0049 was used in Functional assays to study the impact of infected target cell migration on antigen recognition by HIV-specific CD8(+) T cells in a 3D collagen model of peripheral tissue.

Tue Oct 14 00:00:00 EDT 2014

Human regulatory T cells kill tumor cells through granzyme-dependent cytotoxicity upon retargeting with a bispecific antibody.

Cancer immunology research

Choi BD,Gedeon PC,Herndon JE,Archer GE,Reap EA,Sanchez-Perez L,Mitchell DA,Bigner DD,Sampson JH

Published figure using CD4 monoclonal antibody (Product # 14-0049-82) in Flow Cytometry

Sun Sep 01 00:00:00 EDT 2013

Ebf1 and c-Myb repress rag transcription downstream of Stat5 during early B cell development.

Journal of immunology (Baltimore, Md. : 1950)

Timblin GA,Schlissel MS

14-0049 was used in Flow cytometry/Cell sorting to identify a novel pathway responsible for the transcriptional repression of Rag in developing B cells using an in vitro model system.

Fri Nov 01 00:00:00 EDT 2013

Tumor-infiltrating γδ T lymphocytes predict clinical outcome in human breast cancer.

Journal of immunology (Baltimore, Md. : 1950)

Ma C,Zhang Q,Ye J,Wang F,Zhang Y,Wevers E,Schwartz T,Hunborg P,Varvares MA,Hoft DF,Hsueh EC,Peng G

14-0049 was used in Immunohistochemistry to suggest that intratumoural γδ T cells may serve as independent prognostic biomarkers, and potential therapeutic targets for human breast cancer.

Thu Nov 15 00:00:00 EST 2012

Increased frequencies of Th22 cells as well as Th17 cells in the peripheral blood of patients with ankylosing spondylitis and rheumatoid arthritis.

PloS one

Zhang L,Li YG,Li YH,Qi L,Liu XG,Yuan CZ,Hu NW,Ma DX,Li ZF,Yang Q,Li W,Li JM

Published figure using CD4 monoclonal antibody (Product # 14-0049-82) in Flow Cytometry

Fri Jul 27 00:00:00 EDT 2012

Rapid and sensitive detection of rare cancer cells by the coupling of immunomagnetic nanoparticle separation with ELISA analysis.

International journal of nanomedicine

Cheng HY,Lai LJ,Ko FH

14-0049 was used in Magnetic cell separation to develop a rapid and sensitive method for detecting cancer cells occurring at low concentration.

Thu Jan 24 00:00:00 EST 2013

Upregulation of programmed death-1 on T cells and programmed death ligand-1 on monocytes in septic shock patients.

Critical care (London, England)

Zhang Y,Li J,Lou J,Zhou Y,Bo L,Zhu J,Zhu K,Wan X,Cai Z,Deng X

Published figure using CD4 monoclonal antibody (Product # 14-0049-82) in Flow Cytometry

Wed Dec 07 00:00:00 EST 2011

Uncoupling of proliferation and cytokines from suppression within the CD4+CD25+Foxp3+ T-cell compartment in the 1st year of human type 1 diabetes.

Diabetes

Hughson A,Bromberg I,Johnson B,Quataert S,Jospe N,Fowell DJ

14-0049 was used in Flow cytometry/Cell sorting to determine the relative proportions of effector and regulatory T cells in the CD4+CD25+Foxp3+ T-cell compartment in varying type 1 diabetes disease states.

Mon Aug 01 00:00:00 EDT 2011

Modulation of CD4+ T lymphocyte lineage outcomes with targeted, nanoparticle-mediated cytokine delivery.

Molecular pharmaceutics

Park J,Gao W,Whiston R,Strom TB,Metcalfe S,Fahmy TM

Published figure using CD4 monoclonal antibody (Product # 14-0049-82) in Flow Cytometry

Mon Feb 07 00:00:00 EST 2011

Generation of bivalent chromatin domains during cell fate decisions.

Epigenetics & chromatin

De Gobbi M,Garrick D,Lynch M,Vernimmen D,Hughes JR,Goardon N,Luc S,Lower KM,Sloane-Stanley JA,Pina C,Soneji S,Renella R,Enver T,Taylor S,Jacobsen SE,Vyas P,Gibbons RJ,Higgs DR

14-0049 was used in Flow cytometry/Cell sorting to investigate the patterns and levels of histone modifications at bivalent genes, showing the generation of bivalent chromatin domains during cell fate decisions.

Mon Jun 06 00:00:00 EDT 2011

A galectin-3 ligand corrects the impaired function of human CD4 and CD8 tumor-infiltrating lymphocytes and favors tumor rejection in mice.

Cancer research

Demotte N,Wieërs G,Van Der Smissen P,Moser M,Schmidt C,Thielemans K,Squifflet JL,Weynand B,Carrasco J,Lurquin C,Courtoy PJ,van der Bruggen P

14-0049 was used in Flow cytometry/Cell sorting to suggest that a combination of galectin-3 ligands and therapeutic vaccination may induce more tumor regressions than vaccination alone.

Fri Oct 01 00:00:00 EDT 2010

Interferon-gamma promoter hypomethylation and increased expression in chronic periodontitis.

Journal of clinical periodontology

Zhang S,Crivello A,Offenbacher S,Moretti A,Paquette DW,Barros SP

14-0049 was used in Immunofluorescence to determine whether epigenetic modifications in the IFNG promoter are associated with an increase of IFNG transcription at different stages of periodontal diseases.

Mon Nov 01 00:00:00 EDT 2010

The length of and nonhydrophobic residues in the transmembrane domain of dengue virus envelope protein are critical for its retention and assembly in the endoplasmic reticulum.

Journal of virology

Hsieh SC,Tsai WY,Wang WK

14-0049 was used in Immunoprecipitation to investigate determinants in the E protein of dengue virus for its retention and assembly in the endoplasmic reticulum.

Sat May 01 00:00:00 EDT 2010

Sepsis-induced human lymphocyte apoptosis and cytokine production in "humanized" mice.

Journal of leukocyte biology

Unsinger J,McDonough JS,Shultz LD,Ferguson TA,Hotchkiss RS

Published figure using CD4 monoclonal antibody (Product # 14-0049-82) in Flow Cytometry

Sat Aug 01 00:00:00 EDT 2009

B cells and monocytes from patients with active multiple sclerosis exhibit increased surface expression of both HERV-H Env and HERV-W Env, accompanied by increased seroreactivity.

Retrovirology

Brudek T,Christensen T,Aagaard L,Petersen T,Hansen HJ,Møller-Larsen A

Published figure using CD4 monoclonal antibody (Product # 14-0049-82) in Flow Cytometry

Mon Nov 16 00:00:00 EST 2009

Expression of endothelia and lymphocyte adhesion molecules in bronchus-associated lymphoid tissue (BALT) in adult human lung.

Respiratory research

Kawamata N,Xu B,Nishijima H,Aoyama K,Kusumoto M,Takeuchi T,Tei C,Michie SA,Matsuyama T

14-0049 was used in Immunohistochemistry to identify which adhesion molecules recruit specific subsets of lymphocytes into human bronchus-associated lymphoid tissue.

Thu Oct 22 00:00:00 EDT 2009

TCR-induced downregulation of protein tyrosine phosphatase PEST augments secondary T cell responses.

Molecular immunology

Arimura Y,Vang T,Tautz L,Williams S,Mustelin T

14-0049 was used in Magnetic cell separation to investigate how PTP-PEST is down-regulated following T cell activation, and how that can lead to reduced IL-2 secretion following TCR and CD28 stimulation.

Sun Jun 01 00:00:00 EDT 2008

Bacterial superantigens bypass Lck-dependent T cell receptor signaling by activating a Galpha11-dependent, PLC-beta-mediated pathway.

Immunity

Bueno C,Lemke CD,Criado G,Baroja ML,Ferguson SS,Rahman AK,Tsoukas CD,McCormick JK,Madrenas J

14-0049 was used in Functional assays to show that this alternative pathway operates in the absence of Lck-dependent tyrosine-phosphorylation events and was initiated by the TCR-dependent activation of raft-enriched heterotrimeric Galpha11 proteins.

Sat Jul 01 00:00:00 EDT 2006

HIV-1 tropism for the central nervous system: Brain-derived envelope glycoproteins with lower CD4 dependence and reduced sensitivity to a fusion inhibitor.

Virology

Martín-García J,Cao W,Varela-Rohena A,Plassmeyer ML,González-Scarano F

14-0049 was used in Flow cytometry/Cell sorting to suggest an increased affinity for CD4 of brain-derived envelopes that may have originated from adaptation to replication in microglial cells.

Wed Mar 01 00:00:00 EST 2006

Interaction with CD4 and antibodies to CD4-induced epitopes of the envelope gp120 from a microglial cell-adapted human immunodeficiency virus type 1 isolate.

Journal of virology

Martín-García J,Cocklin S,Chaiken IM,González-Scarano F

14-0049-82 was used in Inhibition Assays to investigate whether phenotypic changes between HIV-1(Bori-15) and HIV-1(Bori) were related to a different interaction of their soluble monomeric gp120 proteins with CD4 or 17b.

Wed Jun 01 00:00:00 EDT 2005

Bioinformatics

Protein Aliases: CD4; CD4 antigen (p55); CD4 antigen p55; CD4 receptor; cd4a; fCD4; Leu-3; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4

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Gene Aliases: CD4; CD4mut

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UniProt ID: (Human) P01730

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Entrez Gene ID: (Human) 920

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Function(s)

virus receptor activity glycoprotein binding receptor activity transmembrane signaling receptor activity extracellular matrix structural constituent protein binding zinc ion binding coreceptor activity immunoglobulin binding enzyme binding protein kinase binding MHC class II protein binding protein homodimerization activity protein tyrosine kinase binding

Process(es)

cytokine production adaptive immune response induction by virus of host cell-cell fusion immune response signal transduction cell surface receptor signaling pathway enzyme linked receptor protein signaling pathway transmembrane receptor protein tyrosine kinase signaling pathway positive regulation of calcium ion transport into cytosol fusion of virus membrane with host plasma membrane T cell differentiation entry into host cell T cell costimulation response to estradiol maintenance of protein location in cell response to vitamin D helper T cell enhancement of adaptive immune response positive regulation of T cell proliferation T cell selection positive regulation of interleukin-2 biosynthetic process positive regulation of protein kinase activity regulation of defense response to virus by virus positive regulation of peptidyl-tyrosine phosphorylation defense response to Gram-negative bacterium positive regulation of calcium-mediated signaling T cell receptor signaling pathway regulation of T cell activation

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CD4 Monoclonal Antibody (RPA-T4), eBioscience™

CD4 Antibody (14-0049-82) in Flow

Product Details

14-0049-82

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Neutralization (Neu)

Functional Assay (Functional)

Inhibition Assays (IA)

Fluorescence Resonance Energy Transfer (FRET)

Miscellaneous PubMed (Misc)

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