Invitrogen

CD4 Monoclonal Antibody (RPA-T4), APC-eFluor™ 780, eBioscience™

FIGURE: 1 / 1

CD4 Antibody (47-0049-42) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD8a APC (Product # 17-0088-42) and Mouse IgG1 kappa Isotype Control APC-eFluor® 780 (Product # 47-4714-82) (left) or Anti-Human CD4 APC-eFluor® 780 (right). Cells in the lymphocyte gate were used for analysis.

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Product Details

47-0049-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.5 µg)/test

View 17 publications 17 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), APC-eFluor™ 780, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

RPA-T4

Conjugate

APC-eFluor™ 780 APC-eFluor™ 780 APC-eFluor™ 780

Excitation/Emission Max

756/785 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1272044

Product Specific Information

Description: The RPA-T4 monoclonal antibody reacts with human CD4, a 59 kDa cell surface receptor expressed by a majority of thymocytes, subpopulation of mature T cells (T-helper cells) and in low levels on monocytes. CD4 is a receptor for the human immunodeficiency virus (HIV). RPA-T4 blocks HIV binding and mixed lymphocyte reaction. The RPA-T4 antibody recognizes a different epitope than the OKT4 monoclonal antibody, and these antibodies do not cross-block binding to each other's respective epitopes.

Applications Reported: This RPA-T4 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This RPA-T4 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

APC-eFluor 780 emits at 780 nm and is excited with the Red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Light sensitivity: This tandem is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The CD4 antigen is involved in the recognition of MHC class II molecules and is a co-receptor for HIV. CD4 is primarily expressed in a subset of T-lymphocytes, also referred to as T helper cells, but may also be expressed by other cells in the immune system, such as monocytes, macrophages, and dendritic cells. At the tissue level, CD4 expression may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues. CD4 functions to initiate or augment the early phase of T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase, Lck. It may also function as an important mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcripts have been identified in this gene [RefSeq, July 2017].

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

A Novel Homozygous Stop Mutation in IL23R Causes Mendelian Susceptibility to Mycobacterial Disease.

Journal of clinical immunology

Staels F,Lorenzetti F,De Keukeleere K,Willemsen M,Gerbaux M,Neumann J,Tousseyn T,Pasciuto E,De Munter P,Bossuyt X,Gijsbers R,Liston A,Humblet-Baron S,Schrijvers R

47-0049-42 was used in Flow cytometry/Cell sorting to demonstrate that impaired IL-23 immunity caused by a homozygous R381X mutation in IL23R underlies MSMD, corroborating earlier findings with a homozygous p.C115Y IL23R mutation.

Tue Nov 01 00:00:00 EDT 2022

IL4I1 binds to TMPRSS13 and competes with SARS-CoV-2 spike.

Frontiers in immunology

Gatineau J,Nidercorne C,Dupont A,Puiffe ML,Cohen JL,Molinier-Frenkel V,Niedergang F,Castellano F

47-0049-42 was used in Flow cytometry/Cell sorting to identify regions of homology between IL4I1 and spike and demonstrate competition between the two proteins for TMPRSS13 binding.

Fri Sep 23 00:00:00 EDT 2022

Ex vivo assays show human gamma-delta T cells specific for common allergens are Th1-polarized in allergic donors.

Cell reports methods

Yu ED,Wang E,Garrigan E,Sutherland A,Khalil N,Kearns K,Pham J,Schulten V,Peters B,Frazier A,Sette A,da Silva Antunes R

47-0049-42 was used in flow cytometry to support the existence of allergen-reactive γδ T cells and their potential use in rebalancing dysregulated Th2 responses in allergic diseases.

Mon Dec 19 00:00:00 EST 2022

Epitope length variants balance protective immune responses and viral escape in HIV-1 infection.

Cell reports

Pymm P,Tenzer S,Wee E,Weimershaus M,Burgevin A,Kollnberger S,Gerstoft J,Josephs TM,Ladell K,McLaren JE,Appay V,Price DA,Fugger L,Bell JI,Schild H,van Endert P,Harkiolaki M,Iversen AKN

47-0049-42 was used in Flow cytometry/Cell sorting to identify a viral escape mechanism from CTL and NK responses based on differential antigen processing and peptide competition.

Tue Mar 01 00:00:00 EST 2022

Regulatory T cells promote cancer immune-escape through integrin αvβ8-mediated TGF-β activation.

Nature communications

Lainé A,Labiad O,Hernandez-Vargas H,This S,Sanlaville A,Léon S,Dalle S,Sheppard D,Travis MA,Paidassi H,Marie JC

47-0049-42 was used in Flow Cytometry to investigate whether TGFβ activation within the tumor depends on different cell types and mechanisms than its production.

Thu Oct 28 00:00:00 EDT 2021

T Cell Responses Induced by Attenuated Flavivirus Vaccination Are Specific and Show Limited Cross-Reactivity with Other Flavivirus Species.

Journal of virology

Grifoni A,Voic H,Dhanda SK,Kidd CK,Brien JD,Buus S,Stryhn A,Durbin AP,Whitehead S,Diehl SA,De Silva AD,Balmaseda A,Harris E,Weiskopf D,Sette A

47-0049 was used in Flow cytometry/Cell sorting to test pools of epitopes derived from dengue (DENV), Zika (ZIKV), Japanese encephalitis (JEV), West Nile (WNV), and yellow fever (YFV) viruses by intracellular cytokine staining (ICS) using peripheral blood mononuclear cells (PBMCs) of individuals naturally exposed to DENV or immunized with DENV (TV005) or YF17D vaccine.

Mon May 04 00:00:00 EDT 2020

NKG2C^pos NK Cells Regulate the Expansion of Cytomegalovirus-Specific CD8 T Cells.

Journal of immunology (Baltimore, Md. : 1950)

Grutza R,Moskorz W,Senff T,Bäcker E,Lindemann M,Zimmermann A,Uhrberg M,Lang PA,Timm J,Cosmovici C

47-0049-42 was used in Flow Cytometry to show that NKG2Cpos NK cells interact with HLA-Ehigh CD8 T cells, which may negatively regulate the expansion of CMV-specific CD8 T cells upon activation.

Mon Jun 01 00:00:00 EDT 2020

Activation-induced Markers Detect Vaccine-Specific CD4⁺ T Cell Responses Not Measured by Assays Conventionally Used in Clinical Trials.

Vaccines

Bowyer G,Rampling T,Powlson J,Morter R,Wright D,Hill AVS,Ewer KJ

Tue Jul 31 00:00:00 EDT 2018

Differences in Peripheral Blood Lymphocytes between Brand-Name and Generic Tacrolimus Used in Stable Liver Transplant Recipients.

Medical principles and practice : international journal of the Kuwait University, Health Science Centre

Kim JM,Kwon CHD,Joh JW,Sinn DH,Choi GS,Park JB,Kang ES,Lee SK

Mon Mar 19 00:00:00 EDT 2018

A Quantitative Analysis of Complexity of Human Pathogen-Specific CD4 T Cell Responses in Healthy M. tuberculosis Infected South Africans.

PLoS pathogens

Lindestam Arlehamn CS,McKinney DM,Carpenter C,Paul S,Rozot V,Makgotlho E,Gregg Y,van Rooyen M,Ernst JD,Hatherill M,Hanekom WA,Peters B,Scriba TJ,Sette A

47-0049 was used in Flow cytometry/Cell sorting to estimate the breadth of T cell responses for the first time in the context of an infection and human population setting.

Fri Jul 01 00:00:00 EDT 2016

Immunodominant Dengue Virus-Specific CD8+ T Cell Responses Are Associated with a Memory PD-1+ Phenotype.

Journal of virology

de Alwis R,Bangs DJ,Angelo MA,Cerpas C,Fernando A,Sidney J,Peters B,Gresh L,Balmaseda A,de Silva AD,Harris E,Sette A,Weiskopf D

47-0049 was used in Flow cytometry/Cell sorting to define DENV-specific CD8(+)T cell responses in general and those of HLA alleles associated with dominant responses.

Sun May 01 00:00:00 EDT 2016

Booster dose after 10 years is recommended following 17DD-YF primary vaccination.

Human vaccines & immunotherapeutics

Campi-Azevedo AC,Costa-Pereira C,Antonelli LR,Fonseca CT,Teixeira-Carvalho A,Villela-Rezende G,Santos RA,Batista MA,Campos FM,Pacheco-Porto L,Melo Júnior OA,Hossell DM,Coelho-dos-Reis JG,Peruhype-Magalhães V,Costa-Silva MF,de Oliveira JG,Farias RH,Noronha TG,Lemos JA,von Doellinger Vdos R,Simões M,de Souza MM,Malaquias LC,Persi HR,Pereira JM,Martins JA,Dornelas-Ribeiro M,Vinhas Ade A,Alves TR,Maia Mde L,Freire Mda S,Martins Rde M,Homma A,Romano AP,Domingues CM,Tauil PL,Vasconcelos PF,Rios M,Caldas IR,Camacho LA,Martins-Filho OA

47004942 was used in flow cytometry to assess the efficacy of a single vaccination of the Yellow Fever vaccine

Tue Dec 13 00:00:00 EST 2016

Genetic and epigenetic variation in the lineage specification of regulatory T cells.

eLife

Arvey A,van der Veeken J,Plitas G,Rich SS,Concannon P,Rudensky AY

47-0049 was used in Flow cytometry/Cell sorting to suggest that a small set of regulatory elements specify the Treg lineage and that genetic variation in Treg cell-specific enhancers may alter Treg cell function contributing to polygenic disease.

Wed Oct 28 00:00:00 EDT 2015

Automatic Generation of Validated Specific Epitope Sets.

Journal of immunology research

Carrasco Pro S,Sidney J,Paul S,Lindestam Arlehamn C,Weiskopf D,Peters B,Sette A

47-0049 was used in Flow cytometry/Cell sorting to develop a method to automatically select reference epitope sets according to a categorization system employed by the IEDB.

Mon Sep 26 00:00:00 EDT 2016

Transcriptional profile of tuberculosis antigen-specific T cells reveals novel multifunctional features.

Journal of immunology (Baltimore, Md. : 1950)

Arlehamn CL,Seumois G,Gerasimova A,Huang C,Fu Z,Yue X,Sette A,Vijayanand P,Peters B

47-0049 was used in Flow cytometry/Cell sorting to study the role of CD4(+) T cells in latent tuberculosis infection, showing that tuberculosis antigen-specific T cells have novel multifunctional features.

Mon Sep 15 00:00:00 EDT 2014

Quantitative reconstruction of leukocyte subsets using DNA methylation.

Genome biology

Accomando WP,Wiencke JK,Houseman EA,Nelson HH,Kelsey KT

47-0049 was used in Flow cytometry/Cell sorting to study how precise immune cell differential estimates can be reconstructed using only DNA rather than whole cells.

Wed Mar 05 00:00:00 EST 2014

Association of a NOD2 gene polymorphism and T-helper 17 cells with presumed ocular toxoplasmosis.

The Journal of infectious diseases

Dutra MS,Béla SR,Peixoto-Rangel AL,Fakiola M,Cruz AG,Gazzinelli A,Quites HF,Bahia-Oliveira LM,Peixe RG,Campos WR,Higino-Rocha AC,Miller NE,Blackwell JM,Antonelli LR,Gazzinelli RT

47-0049 was used in Flow cytometry/Cell sorting to suggest that NOD2 influences the production of IL-17A by CD4 T lymphocytes and might contribute to the development of ocular toxoplasmosis.

Tue Jan 01 00:00:00 EST 2013

Bioinformatics

Protein Aliases: CD4; CD4 antigen (p55); CD4 antigen p55; CD4 receptor; cd4a; fCD4; Leu-3; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4

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Gene Aliases: CD4; CD4mut

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UniProt ID: (Human) P01730

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Entrez Gene ID: (Human) 920

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Function(s)

virus receptor activity glycoprotein binding receptor activity transmembrane signaling receptor activity extracellular matrix structural constituent protein binding zinc ion binding coreceptor activity immunoglobulin binding enzyme binding protein kinase binding MHC class II protein binding protein homodimerization activity protein tyrosine kinase binding

Process(es)

cytokine production adaptive immune response induction by virus of host cell-cell fusion immune response signal transduction cell surface receptor signaling pathway enzyme linked receptor protein signaling pathway transmembrane receptor protein tyrosine kinase signaling pathway positive regulation of calcium ion transport into cytosol fusion of virus membrane with host plasma membrane T cell differentiation entry into host cell T cell costimulation response to estradiol maintenance of protein location in cell response to vitamin D helper T cell enhancement of adaptive immune response positive regulation of T cell proliferation T cell selection positive regulation of interleukin-2 biosynthetic process positive regulation of protein kinase activity regulation of defense response to virus by virus positive regulation of peptidyl-tyrosine phosphorylation defense response to Gram-negative bacterium positive regulation of calcium-mediated signaling T cell receptor signaling pathway regulation of T cell activation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD4 Monoclonal Antibody (RPA-T4), APC-eFluor™ 780, eBioscience™

CD4 Antibody (47-0049-42) in Flow

Product Details

47-0049-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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