Invitrogen

CD27 Monoclonal Antibody (O323), eBioscience™

FIGURE: 1 / 25

CD27 Antibody (14-0279-82) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD27 PE (left) and APC (right). Appropriate isotype controls were used (open histogram). Cells in the lymphocyte population were used for analysis.

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Product Details

14-0279-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

1 µg/test

View 22 publications 22 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse, Non-human primate, Rhesus monkey

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

O323

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_467186

Product Specific Information

Description: The O323 monoclonal antibody reacts with human CD27, a lymphocyte-specific member of the TNFR superfamily. CD27 is expressed by a subset of thymocytes and virtually all mature T cells and is upregulated upon T-cell stimulation. CD27 binds to CD70, and through this interaction, plays an important role in T cell-B cell interaction.

Applications Reported: The O323 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The O323 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD27 is a 50 kDa member of the tumor necrosis factor (TNF) receptor superfamily that includes CD40 and CD30. The TNF superfamily members are known for the regulation of cell proliferation and death. In contrast to the expression of other TNFR/TNF family members, expression of CD27 and its ligand CD70 is predominantly confined to lymphocytes. High expression levels of CD27 appear to be dependent on proper ligation of antigen receptors. CD70 expression requires additional co-stimulatory and/or pro-inflammatory signals. CD27 is expressed as a disulfide-linked homodimer on mature thymocytes, peripheral blood T cells and a subpopulation of B cells. Activation of T cells via TCR-CD3 complex results in upregulation of CD27 expression on the plasma membrane as well as in the release of its soluble 28-32 kDa form, sCD27, detected in the plasma, urine or spinal fluid. Soluble CD27 is an important prognostic marker of acute and chronic B cell malignancies. RgpA, a cystein proteinase, although activating T cells through the protease-activated receptors (PARs), degradates CD27 and counteracts T cell activation mediated by CD27 and its ligand CD70. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor. Diseases associated with CD27 dysfunction include Lymphoproliferative Syndrome 2 and Autosomal Recessive Lymphoproliferative Syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

B-cell targeting with anti-CD38 daratumumab: implications for differentiation and memory responses.

Life science alliance

Verhoeven D,Grinwis L,Marsman C,Jansen MH,Van Leeuwen EM,Kuijpers TW

Published figure using CD27 monoclonal antibody (Product # 14-0279-82) in Flow Cytometry

Fri Sep 01 00:00:00 EDT 2023

Oxygen level is a critical regulator of human B cell differentiation and IgG class switch recombination.

Frontiers in immunology

Koers J,Marsman C,Steuten J,Tol S,Derksen NIL,Ten Brinke A,van Ham SM,Rispens T

Published figure using CD27 monoclonal antibody (Product # 14-0279-82) in Flow Cytometry

Tue Jan 03 00:00:00 EST 2023

Expression of CD27 and CD28 on γδ T cells from the peripheral blood of patients with allergic rhinitis.

Experimental and therapeutic medicine

Wang Q,Sun Q,Chen Q,Li H,Liu D

Published figure using CD27 monoclonal antibody (Product # 14-0279-82) in Flow Cytometry

Tue Dec 01 00:00:00 EST 2020

Developing Human Skin Contains Lymphocytes Demonstrating a Memory Signature.

Cell reports. Medicine

Dhariwala MO,Karthikeyan D,Vasquez KS,Farhat S,Weckel A,Taravati K,Leitner EG,Clancy S,Pauli M,Piper ML,Cohen JN,Ashouri JF,Lowe MM,Rosenblum MD,Scharschmidt TC

Published figure using CD27 monoclonal antibody (Product # 14-0279-82) in Flow Cytometry

Tue Nov 17 00:00:00 EST 2020

More than one antibody of individual B cells revealed by single-cell immune profiling.

Cell discovery

Shi Z,Zhang Q,Yan H,Yang Y,Wang P,Zhang Y,Deng Z,Yu M,Zhou W,Wang Q,Yang X,Mo X,Zhang C,Huang J,Dai H,Sun B,Zhao Y,Zhang L,Yang YG,Qiu X

Published figure using CD27 monoclonal antibody (Product # 14-0279-82) in Flow Cytometry

Fri Oct 20 00:00:00 EDT 2023

The Calcineurin Inhibitor Tacrolimus Specifically Suppresses Human T Follicular Helper Cells.

Frontiers in immunology

Wallin EF,Hill DL,Linterman MA,Wood KJ

14-0279 was used in Flow cytometry/Cell sorting to study the impact of the immunosuppressive agent Tacrolimuson T follicular helper cells differentiationand function in transplant recipients.

Fri Aug 16 00:00:00 EDT 2019

Longitudinal transcriptomic characterization of the immune response to acute hepatitis C virus infection in patients with spontaneous viral clearance.

PLoS pathogens

Rosenberg BR,Depla M,Freije CA,Gaucher D,Mazouz S,Boisvert M,Bédard N,Bruneau J,Rice CM,Shoukry NH

14-0279 was used in Flow cytometry/Cell sorting to represent the first longitudinal transcriptomic characterization of human immune function in PBMC during acute HCV infection and identify several dynamically regulated features of the complex response to natural HCV exposure.

Sat Sep 01 00:00:00 EDT 2018

Perturbed CD8⁺ T cell immunity across universal influenza epitopes in the elderly.

Journal of leukocyte biology

Nguyen THO,Sant S,Bird NL,Grant EJ,Clemens EB,Koutsakos M,Valkenburg SA,Gras S,Lappas M,Jaworowski A,Crowe J,Loh L,Kedzierska K

Published figure using CD27 monoclonal antibody (Product # 14-0279-82) in Flow Cytometry

Thu Feb 01 00:00:00 EST 2018

Clonally diverse CD38⁺HLA-DR⁺CD8⁺ T cells persist during fatal H7N9 disease.

Nature communications

Wang Z,Zhu L,Nguyen THO,Wan Y,Sant S,Quiñones-Parra SM,Crawford JC,Eltahla AA,Rizzetto S,Bull RA,Qiu C,Koutsakos M,Clemens EB,Loh L,Chen T,Liu L,Cao P,Ren Y,Kedzierski L,Kotsimbos T,McCaw JM,La Gruta NL,Turner SJ,Cheng AC,Luciani F,Zhang X,Doherty PC,Thomas PG,Xu J,Kedzierska K

Published figure using CD27 monoclonal antibody (Product # 14-0279-82) in Flow Cytometry

Mon Feb 26 00:00:00 EST 2018

Maintenance of the EBV-specific CD8⁺ TCRαβ repertoire in immunosuppressed lung transplant recipients.

Immunology and cell biology

Nguyen TH,Bird NL,Grant EJ,Miles JJ,Thomas PG,Kotsimbos TC,Mifsud NA,Kedzierska K

Published figure using CD27 monoclonal antibody (Product # 14-0279-82) in Flow Cytometry

Sun Jan 01 00:00:00 EST 2017

Abnormal phenotypic features of IgM+B cell subsets in patients with chronic hepatitis C virus infection.

Experimental and therapeutic medicine

Kong F,Feng B,Zhang H,Rao H,Wang J,Cong X,Wei L

Published figure using CD27 monoclonal antibody (Product # 14-0279-82) in Flow Cytometry

Tue Aug 01 00:00:00 EDT 2017

Antigen receptor-redirected T cells derived from hematopoietic precursor cells lack expression of the endogenous TCR/CD3 receptor and exhibit specific antitumor capacities.

Oncoimmunology

Van Caeneghem Y,De Munter S,Tieppo P,Goetgeluk G,Weening K,Verstichel G,Bonte S,Taghon T,Leclercq G,Kerre T,Debets R,Vermijlen D,Abken H,Vandekerckhove B

Published figure using CD27 monoclonal antibody (Product # 14-0279-82) in Flow Cytometry

Tue Jan 12 00:00:00 EST 2021

Cord blood Vγ2Vδ2 T cells provide a molecular marker for the influence of pregnancy-associated malaria on neonatal immunity.

The Journal of infectious diseases

Cairo C,Longinaker N,Cappelli G,Leke RG,Ondo MM,Djokam R,Fogako J,Leke RJ,Sagnia B,Sosso S,Colizzi V,Pauza CD

14-0279 was used in Flow cytometry/Cell sorting to study Vγ2Vδ2 lymphocyte responses in neonates exposed to P. falciparum metabolites.

Thu May 15 00:00:00 EDT 2014

Follicular helper T cells serve as the major CD4 T cell compartment for HIV-1 infection, replication, and production.

The Journal of experimental medicine

Perreau M,Savoye AL,De Crignis E,Corpataux JM,Cubas R,Haddad EK,De Leval L,Graziosi C,Pantaleo G

14-0279 was used in Flow cytometry/Cell sorting to demonstrate that T follicular helper cells serve as the major CD4 T-cell compartment for HIV infection, replication, and production.

Mon Jan 14 00:00:00 EST 2013

Long-lived Plasmodium falciparum specific memory B cells in naturally exposed Swedish travelers.

European journal of immunology

Ndungu FM,Lundblom K,Rono J,Illingworth J,Eriksson S,Färnert A

Published figure using CD27 monoclonal antibody (Product # 14-0279-82) in Flow Cytometry

Fri Nov 01 00:00:00 EDT 2013

Chronic exposure to Plasmodium falciparum is associated with phenotypic evidence of B and T cell exhaustion.

Journal of immunology (Baltimore, Md. : 1950)

Illingworth J,Butler NS,Roetynck S,Mwacharo J,Pierce SK,Bejon P,Crompton PD,Marsh K,Ndungu FM

Published figure using CD27 monoclonal antibody (Product # 14-0279-82) in Flow Cytometry

Fri Feb 01 00:00:00 EST 2013

Rate of AIDS progression is associated with gastrointestinal dysfunction in simian immunodeficiency virus-infected pigtail macaques.

Journal of immunology (Baltimore, Md. : 1950)

Canary LA,Vinton CL,Morcock DR,Pierce JB,Estes JD,Brenchley JM,Klatt NR

14-0279 was used in Flow cytometry/Cell sorting to determine whether pre-existing GI-associated mucosal damage affects the progression of AIDS following HIV infection.

Fri Mar 15 00:00:00 EDT 2013

OMIP-013: differentiation of human T-cells.

Cytometry. Part A : the journal of the International Society for Analytical Cytology

Mahnke YD,Beddall MH,Roederer M

Published figure using CD27 monoclonal antibody (Product # 14-0279-82) in Flow Cytometry

Thu Nov 01 00:00:00 EDT 2012

Long-term immunologically competent human peripheral lymphoid tissue cultures in a 3D bioreactor.

Biotechnology and bioengineering

Kuzin I,Sun H,Moshkani S,Feng C,Mantalaris A,Wu JH,Bottaro A

Published figure using CD27 monoclonal antibody (Product # 14-0279-82) in Flow Cytometry

Wed Jun 01 00:00:00 EDT 2011

Antigen-specific monoclonal antibodies isolated from B cells expressing constitutively active STAT5.

PloS one

Scheeren FA,van Geelen CM,Yasuda E,Spits H,Beaumont T

14-0279 was used in Flow cytometry/Cell sorting to show that STAT5 overexpression can be employed to isolate antigen specific antibodies from human memory B cells.

Fri Apr 15 00:00:00 EDT 2011

Gammadelta T cell immune manipulation during chronic phase of simian-human immunodeficiency virus infection [corrected] confers immunological benefits.

Journal of immunology (Baltimore, Md. : 1950)

Ali Z,Yan L,Plagman N,Reichenberg A,Hintz M,Jomaa H,Villinger F,Chen ZW

14-0279-82 was used in Flow Cytometry to investigate whether a well-defined phosphoantigen-4-hydroxy-3-methyl-but-2-enyl pyrophosphate/IL-2 therapeutic regimen can overcome HIV-mediated immune suppression to massively expand polyfunctional Vgamma2Vdelta2 T cells.

Thu Oct 15 00:00:00 EDT 2009

Wiskott-Aldrich syndrome protein is required for regulatory T cell homeostasis.

The Journal of clinical investigation

Humblet-Baron S,Sather B,Anover S,Becker-Herman S,Kasprowicz DJ,Khim S,Nguyen T,Hudkins-Loya K,Alpers CE,Ziegler SF,Ochs H,Torgerson T,Campbell DJ,Rawlings DJ

14-0279 was used in Flow cytometry/Cell sorting to investigate whether alterations in Treg function might explain Wiskott-Aldrich syndrome pathology, showing that WASp is required for Treg homeostasis.

Thu Feb 01 00:00:00 EST 2007

Bioinformatics

Protein Aliases: CD27; CD27 antigen; CD27L receptor; LPFS2; sCD27; soluble CD27; T cell activation antigen S152; T-cell activation antigen CD27; T14; TNFSF7; Tumor necrosis factor receptor superfamily member 7; tumor necrosis factor receptor superfamily, member 7

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Gene Aliases: CD27; S152; S152. LPFS2; T14; TNFRSF7; Tp55

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UniProt ID: (Human) P26842

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Entrez Gene ID: (Human) 939

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Function(s)

transmembrane signaling receptor activity tumor necrosis factor-activated receptor activity protein binding cysteine-type endopeptidase inhibitor activity involved in apoptotic process

Process(es)

inflammatory response immune response cell surface receptor signaling pathway brain development release of cytoplasmic sequestered NF-kappaB extrinsic apoptotic signaling pathway via death domain receptors immunoglobulin mediated immune response response to lipopolysaccharide tumor necrosis factor-mediated signaling pathway regulation of cell proliferation negative regulation of apoptotic process negative regulation of cysteine-type endopeptidase activity involved in apoptotic process positive regulation of MAPK cascade response to ethanol positive regulation of B cell differentiation positive regulation of JNK cascade negative regulation of T cell apoptotic process extrinsic apoptotic signaling pathway

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD27 Monoclonal Antibody (O323), eBioscience™

CD27 Antibody (14-0279-82) in Flow

Product Details

14-0279-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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