Invitrogen

CD27 Monoclonal Antibody (O323), PE-Cyanine7, eBioscience™

FIGURE: 1 / 21

CD27 Antibody (25-0279-42) in Flow

Staining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control PE-Cyanine7 (Product # 25-4714-80) (open histogram) or Anti-Human CD27 PE-Cyanine7 (filled histogram). Cells in the lymphocyte gate were used for analysis.

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Product Details

25-0279-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.25 µg)/test

View 29 publications 29 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse, Non-human primate, Rhesus monkey

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE-Cyanine7, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

O323

Conjugate

PE-Cyanine7 PE-Cyanine7 PE-Cyanine7

Excitation/Emission Max

569/780 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1724039

Product Specific Information

Description: The O323 monoclonal antibody reacts with human CD27, a lymphocyte-specific member of the TNFR superfamily. CD27 is expressed by a subset of thymocytes and virtually all mature T cells and is upregulated upon T-cell stimulation. CD27 binds to CD70, and through this interaction, plays an important role in T cell-B cell interaction.

Applications Reported: This O323 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This O323 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD27 is a 50 kDa member of the tumor necrosis factor (TNF) receptor superfamily that includes CD40 and CD30. The TNF superfamily members are known for the regulation of cell proliferation and death. In contrast to the expression of other TNFR/TNF family members, expression of CD27 and its ligand CD70 is predominantly confined to lymphocytes. High expression levels of CD27 appear to be dependent on proper ligation of antigen receptors. CD70 expression requires additional co-stimulatory and/or pro-inflammatory signals. CD27 is expressed as a disulfide-linked homodimer on mature thymocytes, peripheral blood T cells and a subpopulation of B cells. Activation of T cells via TCR-CD3 complex results in upregulation of CD27 expression on the plasma membrane as well as in the release of its soluble 28-32 kDa form, sCD27, detected in the plasma, urine or spinal fluid. Soluble CD27 is an important prognostic marker of acute and chronic B cell malignancies. RgpA, a cystein proteinase, although activating T cells through the protease-activated receptors (PARs), degradates CD27 and counteracts T cell activation mediated by CD27 and its ligand CD70. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor. Diseases associated with CD27 dysfunction include Lymphoproliferative Syndrome 2 and Autosomal Recessive Lymphoproliferative Syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

B-cell targeting with anti-CD38 daratumumab: implications for differentiation and memory responses.

Life science alliance

Verhoeven D,Grinwis L,Marsman C,Jansen MH,Van Leeuwen EM,Kuijpers TW

Published figure using CD27 monoclonal antibody (Product # 25-0279-42) in Flow Cytometry

Fri Sep 01 00:00:00 EDT 2023

Improving naive B cell isolation by absence of CD45RB glycosylation and CD27 expression in combination with BCR isotype.

European journal of immunology

Koers J,Pollastro S,Tol S,Niewold ITG,van Schouwenburg PA,de Vries N,Rispens T

Published figure using CD27 monoclonal antibody (Product # 25-0279-42) in Flow Cytometry

Sat Oct 01 00:00:00 EDT 2022

Improving naive B cell isolation by absence of CD45RB glycosylation and CD27 expression in combination with BCR isotype.

European journal of immunology

Koers J,Pollastro S,Tol S,Niewold ITG,van Schouwenburg PA,de Vries N,Rispens T

Published figure using CD27 monoclonal antibody (Product # 25-0279-42) in Flow Cytometry

Sat Oct 01 00:00:00 EDT 2022

Oxygen level is a critical regulator of human B cell differentiation and IgG class switch recombination.

Frontiers in immunology

Koers J,Marsman C,Steuten J,Tol S,Derksen NIL,Ten Brinke A,van Ham SM,Rispens T

Published figure using CD27 monoclonal antibody (Product # 25-0279-42) in Flow Cytometry

Tue Jan 03 00:00:00 EST 2023

Oxygen level is a critical regulator of human B cell differentiation and IgG class switch recombination.

Frontiers in immunology

Koers J,Marsman C,Steuten J,Tol S,Derksen NIL,Ten Brinke A,van Ham SM,Rispens T

Published figure using CD27 monoclonal antibody (Product # 25-0279-42) in Flow Cytometry

Tue Jan 03 00:00:00 EST 2023

Dynamics of NK, CD8 and Tfh cell mediated the production of cytokines and antiviral antibodies in Chinese patients with moderate COVID-19.

Journal of cellular and molecular medicine

Yan L,Cai B,Li Y,Wang MJ,An YF,Deng R,Li DD,Wang LC,Xu H,Gao XD,Wang LL

25-0279-42 was used in Flow Cytometry to study the changes of NK, T- and B-cell subsets, inflammatory cytokines and virus-specific antibodies in patients with moderate COVID-19. Subsets were analysed using flow cytometry, cytokines and SARS-CoV-2-specific antibodies were analysed using an electrochemiluminescence immunoassay.

Tue Dec 01 00:00:00 EST 2020

Expression of CD27 and CD28 on γδ T cells from the peripheral blood of patients with allergic rhinitis.

Experimental and therapeutic medicine

Wang Q,Sun Q,Chen Q,Li H,Liu D

Published figure using CD27 monoclonal antibody (Product # 25-0279-42) in Flow Cytometry

Tue Dec 01 00:00:00 EST 2020

Developing Human Skin Contains Lymphocytes Demonstrating a Memory Signature.

Cell reports. Medicine

Dhariwala MO,Karthikeyan D,Vasquez KS,Farhat S,Weckel A,Taravati K,Leitner EG,Clancy S,Pauli M,Piper ML,Cohen JN,Ashouri JF,Lowe MM,Rosenblum MD,Scharschmidt TC

Published figure using CD27 monoclonal antibody (Product # 25-0279-42) in Flow Cytometry

Tue Nov 17 00:00:00 EST 2020

More than one antibody of individual B cells revealed by single-cell immune profiling.

Cell discovery

Shi Z,Zhang Q,Yan H,Yang Y,Wang P,Zhang Y,Deng Z,Yu M,Zhou W,Wang Q,Yang X,Mo X,Zhang C,Huang J,Dai H,Sun B,Zhao Y,Zhang L,Yang YG,Qiu X

Published figure using CD27 monoclonal antibody (Product # 25-0279-42) in Flow Cytometry

Fri Oct 20 00:00:00 EDT 2023

CD19⁺CD24^hiCD38^hi B Cells Are Expanded in Juvenile Dermatomyositis and Exhibit a Pro-Inflammatory Phenotype After Activation Through Toll-Like Receptor 7 and Interferon-α.

Frontiers in immunology

Piper CJM,Wilkinson MGL,Deakin CT,Otto GW,Dowle S,Duurland CL,Adams S,Marasco E,Rosser EC,Radziszewska A,Carsetti R,Ioannou Y,Beales PL,Kelberman D,Isenberg DA,Mauri C,Nistala K,Wedderburn LR

25-0279 was used in Flow cytometry/Cell sorting to identify that immature transitional B cells were significantly expanded in active JDM, actively dividing, and correlated positively with disease activity.

Wed Nov 20 00:00:00 EST 2019

Perturbed CD8⁺ T cell immunity across universal influenza epitopes in the elderly.

Journal of leukocyte biology

Nguyen THO,Sant S,Bird NL,Grant EJ,Clemens EB,Koutsakos M,Valkenburg SA,Gras S,Lappas M,Jaworowski A,Crowe J,Loh L,Kedzierska K

Published figure using CD27 monoclonal antibody (Product # 25-0279-42) in Flow Cytometry

Thu Feb 01 00:00:00 EST 2018

Clonally diverse CD38⁺HLA-DR⁺CD8⁺ T cells persist during fatal H7N9 disease.

Nature communications

Wang Z,Zhu L,Nguyen THO,Wan Y,Sant S,Quiñones-Parra SM,Crawford JC,Eltahla AA,Rizzetto S,Bull RA,Qiu C,Koutsakos M,Clemens EB,Loh L,Chen T,Liu L,Cao P,Ren Y,Kedzierski L,Kotsimbos T,McCaw JM,La Gruta NL,Turner SJ,Cheng AC,Luciani F,Zhang X,Doherty PC,Thomas PG,Xu J,Kedzierska K

25-0279 was used in Flow cytometry/Cell sorting to characterise the circulating CD8+T-cell profiles from patients hospitalised with avian H7N9, seasonal influenza A virus, and influenza vaccinees.

Mon Feb 26 00:00:00 EST 2018

Maintenance of the EBV-specific CD8⁺ TCRαβ repertoire in immunosuppressed lung transplant recipients.

Immunology and cell biology

Nguyen TH,Bird NL,Grant EJ,Miles JJ,Thomas PG,Kotsimbos TC,Mifsud NA,Kedzierska K

Published figure using CD27 monoclonal antibody (Product # 25-0279-42) in Flow Cytometry

Sun Jan 01 00:00:00 EST 2017

Abnormal phenotypic features of IgM+B cell subsets in patients with chronic hepatitis C virus infection.

Experimental and therapeutic medicine

Kong F,Feng B,Zhang H,Rao H,Wang J,Cong X,Wei L

Published figure using CD27 monoclonal antibody (Product # 25-0279-42) in Flow Cytometry

Tue Aug 01 00:00:00 EDT 2017

Antigen receptor-redirected T cells derived from hematopoietic precursor cells lack expression of the endogenous TCR/CD3 receptor and exhibit specific antitumor capacities.

Oncoimmunology

Van Caeneghem Y,De Munter S,Tieppo P,Goetgeluk G,Weening K,Verstichel G,Bonte S,Taghon T,Leclercq G,Kerre T,Debets R,Vermijlen D,Abken H,Vandekerckhove B

Published figure using CD27 monoclonal antibody (Product # 25-0279-42) in Flow Cytometry

Tue Jan 12 00:00:00 EST 2021

IL-15 enhances the anti-tumor activity of trastuzumab against breast cancer cells but causes fatal side effects in humanized tumor mice (HTM).

Oncotarget

Wege AK,Weber F,Kroemer A,Ortmann O,Nimmerjahn F,Brockhoff G

25-0279 was used in Flow cytometry/Cell sorting to evaluate the effect of IL-15 in trastuzumab treated humanised tumour mice and HER2 positive breast cancer cells.

Tue Jan 10 00:00:00 EST 2017

High-dimensional assessment of B-cell responses to quadrivalent meningococcal conjugate and plain polysaccharide vaccine.

Genome medicine

O'Connor D,Clutterbuck EA,Thompson AJ,Snape MD,Ramasamy MN,Kelly DF,Pollard AJ

25027942 was used in flow cytometry to analyze B-cell responses to meningococcal polysaccharide and conjugate vaccines against Neisseria meningitidis

Mon Jan 30 00:00:00 EST 2017

Restricted isotype, distinct variable gene usage, and high rate of gp120 specificity of HIV-1 envelope-specific B cells in colostrum compared with those in blood of HIV-1-infected, lactating African women.

Mucosal immunology

Sacha CR,Vandergrift N,Jeffries TL,McGuire E,Fouda GG,Liebl B,Marshall DJ,Gurley TC,Stiegel L,Whitesides JF,Friedman J,Badiabo A,Foulger A,Yates NL,Tomaras GD,Kepler TB,Liao HX,Haynes BF,Moody MA,Permar SR

25-0279 was used in Flow cytometry/Cell sorting to suggest HIV-1-specific IgG1-secreting memory B cells selectively home to the mammary gland.

Sun Mar 01 00:00:00 EST 2015

Rapid Development of gp120-Focused Neutralizing B Cell Responses during Acute Simian Immunodeficiency Virus Infection of African Green Monkeys.

Journal of virology

Amos JD,Himes JE,Armand L,Gurley TC,Martinez DR,Colvin L,Beck K,Overman RG,Liao HX,Moody MA,Permar SR

25-0279 was used in Flow cytometry/Cell sorting to compare the envelope (env)-specific antibody responses of african green monkeys and rhesus macaques to simian immunodeficiency virus infection.

Tue Sep 01 00:00:00 EDT 2015

Reduced expression of CD27 by collagenase treatment: implications for interpreting b cell data in tissues.

PloS one

Shen C,Xu H,Alvarez X,Lackner AA,Veazey RS,Wang X

25-0279 was used in Flow cytometry/Cell sorting to suggest that CD27 may be a memory marker for B cells, but not for T cells, since essentially all CD3 T cells expressed CD27.

Wed Jan 13 00:00:00 EST 2016

Phenotypic, genomic and functional characterization reveals no differences between CD138++ and CD138low subpopulations in multiple myeloma cell lines.

PloS one

Paíno T,Sarasquete ME,Paiva B,Krzeminski P,San-Segundo L,Corchete LA,Redondo A,Garayoa M,García-Sanz R,Gutiérrez NC,Ocio EM,San-Miguel JF

25-0279 was used in Flow cytometry/Cell sorting to investigate the phenotype of multiple myeloma cancer stem cells, showing no differences between CD138++ and CD138low subpopulations.

Tue Jan 20 00:00:00 EST 2015

Follicular helper T cells serve as the major CD4 T cell compartment for HIV-1 infection, replication, and production.

The Journal of experimental medicine

Perreau M,Savoye AL,De Crignis E,Corpataux JM,Cubas R,Haddad EK,De Leval L,Graziosi C,Pantaleo G

25-0279 was used in Flow cytometry/Cell sorting to demonstrate that T follicular helper cells serve as the major CD4 T-cell compartment for HIV infection, replication, and production.

Mon Jan 14 00:00:00 EST 2013

Adipose tissue NK cells manifest an activated phenotype in human obesity.

Metabolism: clinical and experimental

O'Rourke RW,Gaston GD,Meyer KA,White AE,Marks DL

25-0279 was used in Flow cytometry/Cell sorting to determine the phenotype of human adipose tissue natural killer cells.

Fri Nov 01 00:00:00 EDT 2013

Rabbit antithymocyte globulin (thymoglobulin) impairs the thymic output of both conventional and regulatory CD4+ T cells after allogeneic hematopoietic stem cell transplantation in adult patients.

Haematologica

Na IK,Wittenbecher F,Dziubianau M,Herholz A,Mensen A,Kunkel D,Blau O,Blau I,Thiel E,Uharek L,Scheibenbogen C,Rieger K,Thiel A

25-0279 was used in Flow cytometry/Cell sorting to evaluate the effects of rabbit antithymocyte globulin-Genzyme™ on T cell kinetics in adult patients undergoing allogeneic hematopoietic stem cell transplantation.

Tue Jan 01 00:00:00 EST 2013

Long-lived Plasmodium falciparum specific memory B cells in naturally exposed Swedish travelers.

European journal of immunology

Ndungu FM,Lundblom K,Rono J,Illingworth J,Eriksson S,Färnert A

Published figure using CD27 monoclonal antibody (Product # 25-0279-42) in Flow Cytometry

Fri Nov 01 00:00:00 EDT 2013

Long-term immune reconstitution of naive and memory t cell pools after haploidentical hematopoietic stem cell transplantation.

Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation

Azevedo RI,Soares MV,Albuquerque AS,Tendeiro R,Soares RS,Martins M,Ligeiro D,Victorino RM,Lacerda JF,Sousa AE

25-0279 was used in Flow cytometry/Cell sorting to show that long-term immune reconstitution was successfully achieved after haploidentical hematopoietic stem cell transplantation, a process that appears to have largely relied on de novo T cell production.

Wed May 01 00:00:00 EDT 2013

Chronic exposure to Plasmodium falciparum is associated with phenotypic evidence of B and T cell exhaustion.

Journal of immunology (Baltimore, Md. : 1950)

Illingworth J,Butler NS,Roetynck S,Mwacharo J,Pierce SK,Bejon P,Crompton PD,Marsh K,Ndungu FM

Published figure using CD27 monoclonal antibody (Product # 25-0279-42) in Flow Cytometry

Fri Feb 01 00:00:00 EST 2013

B cell analysis of ethnic groups in Mali with differential susceptibility to malaria.

Malaria journal

Portugal S,Doumtabe D,Traore B,Miller LH,Troye-Blomberg M,Doumbo OK,Dolo A,Pierce SK,Crompton PD

25-0279 was used in Flow cytometry/Cell sorting to determine the mechanisms underlying the elevated resistance to malaria observed in the Fulani ethnic group.

Fri May 11 00:00:00 EDT 2012

Long-term immunologically competent human peripheral lymphoid tissue cultures in a 3D bioreactor.

Biotechnology and bioengineering

Kuzin I,Sun H,Moshkani S,Feng C,Mantalaris A,Wu JH,Bottaro A

Published figure using CD27 monoclonal antibody (Product # 25-0279-42) in Flow Cytometry

Wed Jun 01 00:00:00 EDT 2011

Vaccine-elicited SIV and HIV envelope-specific IgA and IgG memory B cells in rhesus macaque peripheral blood correlate with functional antibody responses and reduced viremia.

Vaccine

Brocca-Cofano E,McKinnon K,Demberg T,Venzon D,Hidajat R,Xiao P,Daltabuit-Test M,Patterson LJ,Robert-Guroff M

25-0279 was used in Flow cytometry/Cell sorting to evaluate the memory B cell response in immunised rhesus macaques following exposure to simian immunodeficiency virus.

Tue Apr 12 00:00:00 EDT 2011

Chronic lymphocytic leukaemia (CLL) and CLL-type monoclonal B-cell lymphocytosis (MBL) show differential expression of molecules involved in lymphoid tissue homing.

Cytometry. Part B, Clinical cytometry

Rawstron AC,Shingles J,de Tute R,Bennett F,Jack AS,Hillmen P

25-0279 was used in Flow cytometry/Cell sorting to characterise the cell surface markers used to discriminate Chronic lymphocytic leukaemia (CLL)-type monoclonal B-cell lymphocytosis from CLL, or identify the likelihood of stable disease in CLL cases.

Thu Dec 23 00:00:00 EST 2010

Bioinformatics

Protein Aliases: CD27; CD27 antigen; CD27L receptor; LPFS2; sCD27; soluble CD27; T cell activation antigen S152; T-cell activation antigen CD27; T14; TNFSF7; Tumor necrosis factor receptor superfamily member 7; tumor necrosis factor receptor superfamily, member 7

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Gene Aliases: CD27; S152; S152. LPFS2; T14; TNFRSF7; Tp55

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UniProt ID: (Human) P26842

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Entrez Gene ID: (Human) 939

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Function(s)

transmembrane signaling receptor activity tumor necrosis factor-activated receptor activity protein binding cysteine-type endopeptidase inhibitor activity involved in apoptotic process

Process(es)

inflammatory response immune response cell surface receptor signaling pathway brain development release of cytoplasmic sequestered NF-kappaB extrinsic apoptotic signaling pathway via death domain receptors immunoglobulin mediated immune response response to lipopolysaccharide tumor necrosis factor-mediated signaling pathway regulation of cell proliferation negative regulation of apoptotic process negative regulation of cysteine-type endopeptidase activity involved in apoptotic process positive regulation of MAPK cascade response to ethanol positive regulation of B cell differentiation positive regulation of JNK cascade negative regulation of T cell apoptotic process extrinsic apoptotic signaling pathway

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CD27 Monoclonal Antibody (O323), PE-Cyanine7, eBioscience™

CD27 Antibody (25-0279-42) in Flow

Product Details

25-0279-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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