Invitrogen

CD27 Monoclonal Antibody (O323), APC-eFluor™ 780, eBioscience™

FIGURE: 1 / 19

CD27 Antibody (47-0279-42) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD19 FITC (Product # 11-0199-42) and Mouse IgG1 K Isotype Control APC-eFluor® 780 (Product # 47-4714-82) (left) or Anti-Human CD27 APC-eFluor® 780 (right). Cells in the lymphocyte gate were used for analysis.

Product Image Gallery

Product Details

47-0279-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.25 µg)/test

View 32 publications 32 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), APC-eFluor™ 780, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

O323

Conjugate

APC-eFluor™ 780 APC-eFluor™ 780 APC-eFluor™ 780

Excitation/Emission Max

756/785 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1272040

Product Specific Information

Description: The O323 monoclonal antibody reacts with human CD27, a lymphocyte-specific member of the TNFR superfamily. CD27 is expressed by a subset of thymocytes and virtually all mature T cells and is upregulated upon T-cell stimulation. CD27 binds to CD70, and through this interaction, plays an important role in T cell-B cell interaction.

Applications Reported: This O323 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This O323 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

APC-eFluor 780 emits at 780 nm and is excited with the Red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Light sensitivity: This tandem is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD27 is a 50 kDa member of the tumor necrosis factor (TNF) receptor superfamily that includes CD40 and CD30. The TNF superfamily members are known for the regulation of cell proliferation and death. In contrast to the expression of other TNFR/TNF family members, expression of CD27 and its ligand CD70 is predominantly confined to lymphocytes. High expression levels of CD27 appear to be dependent on proper ligation of antigen receptors. CD70 expression requires additional co-stimulatory and/or pro-inflammatory signals. CD27 is expressed as a disulfide-linked homodimer on mature thymocytes, peripheral blood T cells and a subpopulation of B cells. Activation of T cells via TCR-CD3 complex results in upregulation of CD27 expression on the plasma membrane as well as in the release of its soluble 28-32 kDa form, sCD27, detected in the plasma, urine or spinal fluid. Soluble CD27 is an important prognostic marker of acute and chronic B cell malignancies. RgpA, a cystein proteinase, although activating T cells through the protease-activated receptors (PARs), degradates CD27 and counteracts T cell activation mediated by CD27 and its ligand CD70. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor. Diseases associated with CD27 dysfunction include Lymphoproliferative Syndrome 2 and Autosomal Recessive Lymphoproliferative Syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

B-cell targeting with anti-CD38 daratumumab: implications for differentiation and memory responses.

Life science alliance

Verhoeven D,Grinwis L,Marsman C,Jansen MH,Van Leeuwen EM,Kuijpers TW

47-0279-42 was used in Flow Cytometry to investigate the impact of CD38 targeting with daratumumab on B-cell differentiation, revealing significant reductions in proliferation, differentiation, and IgG production.

Fri Sep 01 00:00:00 EDT 2023

B-cell targeting with anti-CD38 daratumumab: implications for differentiation and memory responses.

Life science alliance

Verhoeven D,Grinwis L,Marsman C,Jansen MH,Van Leeuwen EM,Kuijpers TW

Fri Sep 01 00:00:00 EDT 2023

Oxygen level is a critical regulator of human B cell differentiation and IgG class switch recombination.

Frontiers in immunology

Koers J,Marsman C,Steuten J,Tol S,Derksen NIL,Ten Brinke A,van Ham SM,Rispens T

Published figure using CD27 monoclonal antibody (Product # 47-0279-42) in Flow Cytometry

Tue Jan 03 00:00:00 EST 2023

γ9δ2 T-Cell Expansion and Phenotypic Profile Are Reflected in the CDR3δ Repertoire of Healthy Adults.

Frontiers in immunology

Vyborova A,Janssen A,Gatti L,Karaiskaki F,Yonika A,van Dooremalen S,Sanders J,Beringer DX,Straetemans T,Sebestyen Z,Kuball J

47-0279-42 was used in Flow cytometry/Cell sorting to establish consistent repertoire-phenotype associations and justifies stratification for the T-cell phenotype in future research on γ9δ2TCR repertoire dynamics.

Tue Jul 26 00:00:00 EDT 2022

Biomarker correlates with response to NY-ESO-1 TCR T cells in patients with synovial sarcoma.

Nature communications

Gyurdieva A,Zajic S,Chang YF,Houseman EA,Zhong S,Kim J,Nathenson M,Faitg T,Woessner M,Turner DC,Hasan AN,Glod J,Kaplan RN,D'Angelo SP,Araujo DM,Chow WA,Druta M,Demetri GD,Van Tine BA,Grupp SA,Fine GD,Eleftheriadou I

47-0279-42 was used in Flow cytometry/Cell sorting to report potential predictive and pharmacodynamic markers of lete-cel response that may inform LDR, cell dose, and strategies to enhance anticancer efficacy.

Thu Sep 08 00:00:00 EDT 2022

Tertiary lymphoid structures critical for prognosis in endometrial cancer patients.

Nature communications

Horeweg N,Workel HH,Loiero D,Church DN,Vermij L,Léon-Castillo A,Krog RT,de Boer SM,Nout RA,Powell ME,Mileshkin LR,MacKay H,Leary A,Singh N,Jürgenliemk-Schulz IM,Smit VTHBM,Creutzberg CL,Koelzer VH,Nijman HW,Bosse T,de Bruyn M

47-0279-42 was used in Flow Cytometry to investigate the role of B-cells and TLS in endometrial cancer (EC).

Wed Mar 16 00:00:00 EDT 2022

The transcription factor CREB1 is a mechanistic driver of immunogenicity and reduced HIV-1 acquisition following ALVAC vaccination.

Nature immunology

Tomalka JA,Pelletier AN,Fourati S,Latif MB,Sharma A,Furr K,Carlson K,Lifton M,Gonzalez A,Wilkinson P,Franchini G,Parks R,Letvin N,Yates N,Seaton K,Tomaras G,Tartaglia J,Robb ML,Michael NL,Koup R,Haynes B,Santra S,Sekaly RP

47-0279-42 was used in Flow Cytometry to validate CREB1 as a critical driver of vaccine efficacy and highlights that adjuvants that trigger CREB1 signaling may be critical for efficacious HIV-1 vaccines.

Fri Oct 01 00:00:00 EDT 2021

High-throughput compound screen reveals mTOR inhibitors as potential therapeutics to reduce (auto)antibody production by human plasma cells.

European journal of immunology

Tuijnenburg P,Aan de Kerk DJ,Jansen MH,Morris B,Lieftink C,Beijersbergen RL,van Leeuwen EMM,Kuijpers TW

47-0279 was used in Flow cytometry/Cell sorting to explore novel therapeutic targets in signaling pathways essential for plasmablast formation and/or immunoglobulin production.

Wed Jan 01 00:00:00 EST 2020

Expression of CD27 and CD28 on γδ T cells from the peripheral blood of patients with allergic rhinitis.

Experimental and therapeutic medicine

Wang Q,Sun Q,Chen Q,Li H,Liu D

Published figure using CD27 monoclonal antibody (Product # 47-0279-42) in Flow Cytometry

Tue Dec 01 00:00:00 EST 2020

Developing Human Skin Contains Lymphocytes Demonstrating a Memory Signature.

Cell reports. Medicine

Dhariwala MO,Karthikeyan D,Vasquez KS,Farhat S,Weckel A,Taravati K,Leitner EG,Clancy S,Pauli M,Piper ML,Cohen JN,Ashouri JF,Lowe MM,Rosenblum MD,Scharschmidt TC

Published figure using CD27 monoclonal antibody (Product # 47-0279-42) in Flow Cytometry

Tue Nov 17 00:00:00 EST 2020

More than one antibody of individual B cells revealed by single-cell immune profiling.

Cell discovery

Shi Z,Zhang Q,Yan H,Yang Y,Wang P,Zhang Y,Deng Z,Yu M,Zhou W,Wang Q,Yang X,Mo X,Zhang C,Huang J,Dai H,Sun B,Zhao Y,Zhang L,Yang YG,Qiu X

Published figure using CD27 monoclonal antibody (Product # 47-0279-42) in Flow Cytometry

Fri Oct 20 00:00:00 EDT 2023

Differential transcriptome and development of human peripheral plasma cell subsets.

JCI insight

Garimalla S,Nguyen DC,Halliley JL,Tipton C,Rosenberg AF,Fucile CF,Saney CL,Kyu S,Kaminski D,Qian Y,Scheuermann RH,Gibson G,Sanz I,Lee FE

47-0279 was used in Flow cytometry/Cell sorting to study the heterogeneity of human antibody secreting cells after vaccination.

Thu May 02 00:00:00 EDT 2019

Altered Expression of CD300a Inhibitory Receptor on CD4+ T Cells From Human Immunodeficiency Virus-1-Infected Patients: Association With Disease Progression Markers.

Frontiers in immunology

Vitallé J,Terrén I,Gamboa-Urquijo L,Orrantia A,Tarancón-Díez L,Genebat M,Ruiz-Mateos E,Leal M,García-Obregón S,Zenarruzabeitia O,Borrego F

47-0279 was used in Flow cytometry/Cell sorting to investigate the expression of CD300a on CD4+ T cells from human immunodeficiency virus-infected patients on suppressive combined antiretroviral therapy and cART naive patients.

Wed Nov 20 00:00:00 EST 2019

GMP-Grade Manufacturing of T Cells Engineered to Express a Defined γδTCR.

Frontiers in immunology

Straetemans T,Kierkels GJJ,Doorn R,Jansen K,Heijhuurs S,Dos Santos JM,van Muyden ADD,Vie H,Clemenceau B,Raymakers R,de Witte M,Sebestyén Z,Kuball J

47-0279 was used in Flow cytometry/Cell sorting to report on release criteria and the stability of TEG001 drug substance and TEG001 drug product.

Fri Jul 26 00:00:00 EDT 2019

Perturbed CD8⁺ T cell immunity across universal influenza epitopes in the elderly.

Journal of leukocyte biology

Nguyen THO,Sant S,Bird NL,Grant EJ,Clemens EB,Koutsakos M,Valkenburg SA,Gras S,Lappas M,Jaworowski A,Crowe J,Loh L,Kedzierska K

Published figure using CD27 monoclonal antibody (Product # 47-0279-42) in Flow Cytometry

Thu Feb 01 00:00:00 EST 2018

Identifying the Presence of Prostate Cancer in Individuals with PSA Levels <20 ng ml^-1 Using Computational Data Extraction Analysis of High Dimensional Peripheral Blood Flow Cytometric Phenotyping Data.

Frontiers in immunology

Cosma G,McArdle SE,Reeder S,Foulds GA,Hood S,Khan M,Pockley AG

47-0279 was used in Flow cytometry/Cell sorting to present an advanced computational data extraction approach which can identify the presence of prostate cancer in men with PSA levels 20 ng ml-1 on the basis of peripheral blood immune cell profiles.

Wed Nov 20 00:00:00 EST 2019

Novel mechanisms to inhibit HIV reservoir seeding using Jak inhibitors.

PLoS pathogens

Gavegnano C,Brehm JH,Dupuy FP,Talla A,Ribeiro SP,Kulpa DA,Cameron C,Santos S,Hurwitz SJ,Marconi VC,Routy JP,Sabbagh L,Schinazi RF,Sékaly RP

47-0279 was used in Flow cytometry/Cell sorting to evaluate the ability of tofacitinib and ruxolitinib, two FDA-approved Jak inhibitors, to block seeding and maintenance of the HIV reservoir in vitro.

Fri Dec 01 00:00:00 EST 2017

Guidelines for the use of flow cytometry and cell sorting in immunological studies.

European journal of immunology

Cossarizza A,Chang HD,Radbruch A,Akdis M,Andrä I,Annunziato F,Bacher P,Barnaba V,Battistini L,Bauer WM,Baumgart S,Becher B,Beisker W,Berek C,Blanco A,Borsellino G,Boulais PE,Brinkman RR,Büscher M,Busch DH,Bushnell TP,Cao X,Cavani A,Chattopadhyay PK,Cheng Q,Chow S,Clerici M,Cooke A,Cosma A,Cosmi L,Cumano A,Dang VD,Davies D,De Biasi S,Del Zotto G,Della Bella S,Dellabona P,Deniz G,Dessing M,Diefenbach A,Di Santo J,Dieli F,Dolf A,Donnenberg VS,Dörner T,Ehrhardt GRA,Endl E,Engel P,Engelhardt B,Esser C,Everts B,Dreher A,Falk CS,Fehniger TA,Filby A,Fillatreau S,Follo M,Förster I,Foster J,Foulds GA,Frenette PS,Galbraith D,Garbi N,García-Godoy MD,Geginat J,Ghoreschi K,Gibellini L,Goettlinger C,Goodyear CS,Gori A,Grogan J,Gross M,Grützkau A,Grummitt D,Hahn J,Hammer Q,Hauser AE,Haviland DL,Hedley D,Herrera G,Herrmann M,Hiepe F,Holland T,Hombrink P,Houston JP,Hoyer BF,Huang B,Hunter CA,Iannone A,Jäck HM,Jávega B,Jonjic S,Juelke K,Jung S,Kaiser T,Kalina T,Keller B,Khan S,Kienhöfer D,Kroneis T,Kunkel D,Kurts C,Kvistborg P,Lannigan J,Lantz O,Larbi A,LeibundGut-Landmann S,Leipold MD,Levings MK,Litwin V,Liu Y,Lohoff M,Lombardi G,Lopez L,Lovett-Racke A,Lubberts E,Ludewig B,Lugli E,Maecker HT,Martrus G,Matarese G,Maueröder C,McGrath M,McInnes I,Mei HE,Melchers F,Melzer S,Mielenz D,Mills K,Mirrer D,Mjösberg J,Moore J,Moran B,Moretta A,Moretta L,Mosmann TR,Müller S,Müller W,Münz C,Multhoff G,Munoz LE,Murphy KM,Nakayama T,Nasi M,Neudörfl C,Nolan J,Nourshargh S,O'Connor JE,Ouyang W,Oxenius A,Palankar R,Panse I,Peterson P,Peth C,Petriz J,Philips D,Pickl W,Piconese S,Pinti M,Pockley AG,Podolska MJ,Pucillo C,Quataert SA,Radstake TRDJ,Rajwa B,Rebhahn JA,Recktenwald D,Remmerswaal EBM,Rezvani K,Rico LG,Robinson JP,Romagnani C,Rubartelli A,Ruckert B,Ruland J,Sakaguchi S,Sala-de-Oyanguren F,Samstag Y,Sanderson S,Sawitzki B,Scheffold A,Schiemann M,Schildberg F,Schimisky E,Schmid SA,Schmitt S,Schober K,Schüler T,Schulz AR,Schumacher T,Scotta C,Shankey TV,Shemer A,Simon AK,Spidlen J,Stall AM,Stark R,Stehle C,Stein M,Steinmetz T,Stockinger H,Takahama Y,Tarnok A,Tian Z,Toldi G,Tornack J,Traggiai E,Trotter J,Ulrich H,van der Braber M,van Lier RAW,Veldhoen M,Vento-Asturias S,Vieira P,Voehringer D,Volk HD,von Volkmann K,Waisman A,Walker R,Ward MD,Warnatz K,Warth S,Watson JV,Watzl C,Wegener L,Wiedemann A,Wienands J,Willimsky G,Wing J,Wurst P,Yu L,Yue A,Zhang Q,Zhao Y,Ziegler S,Zimmermann J

47-0279 was used in Flow cytometry/Cell sorting to collect the currently accepted best methods for monitoring most of the variation of the major players of immune system.

Sun Oct 01 00:00:00 EDT 2017

Maintenance of the EBV-specific CD8⁺ TCRαβ repertoire in immunosuppressed lung transplant recipients.

Immunology and cell biology

Nguyen TH,Bird NL,Grant EJ,Miles JJ,Thomas PG,Kotsimbos TC,Mifsud NA,Kedzierska K

Published figure using CD27 monoclonal antibody (Product # 47-0279-42) in Flow Cytometry

Sun Jan 01 00:00:00 EST 2017

Decidual T Cells Exhibit a Highly Differentiated Phenotype and Demonstrate Potential Fetal Specificity and a Strong Transcriptional Response to IFN.

Journal of immunology (Baltimore, Md. : 1950)

Powell RM,Lissauer D,Tamblyn J,Beggs A,Cox P,Moss P,Kilby MD

47-0279 was used in Flow cytometry/Cell sorting to investigate the specificity and regulation of CD4+ and CD8+ T cells obtained from human third trimester decidua and demonstrated that decidual CD4+ and CD8+ T cells exhibit a highly differentiated effector memory phenotype in comparison with peripheral blood.

Wed Nov 15 00:00:00 EST 2017

Abnormal phenotypic features of IgM+B cell subsets in patients with chronic hepatitis C virus infection.

Experimental and therapeutic medicine

Kong F,Feng B,Zhang H,Rao H,Wang J,Cong X,Wei L

Published figure using CD27 monoclonal antibody (Product # 47-0279-42) in Flow Cytometry

Tue Aug 01 00:00:00 EDT 2017

Antigen receptor-redirected T cells derived from hematopoietic precursor cells lack expression of the endogenous TCR/CD3 receptor and exhibit specific antitumor capacities.

Oncoimmunology

Van Caeneghem Y,De Munter S,Tieppo P,Goetgeluk G,Weening K,Verstichel G,Bonte S,Taghon T,Leclercq G,Kerre T,Debets R,Vermijlen D,Abken H,Vandekerckhove B

Published figure using CD27 monoclonal antibody (Product # 47-0279-42) in Flow Cytometry

Tue Jan 12 00:00:00 EST 2021

Identification of Genetically Intact HIV-1 Proviruses in Specific CD4⁺ T Cells from Effectively Treated Participants.

Cell reports

Hiener B,Horsburgh BA,Eden JS,Barton K,Schlub TE,Lee E,von Stockenstrom S,Odevall L,Milush JM,Liegler T,Sinclair E,Hoh R,Boritz EA,Douek D,Fromentin R,Chomont N,Deeks SG,Hecht FM,Palmer S

47-0279 was used in Flow cytometry/Cell sorting to develop the Full-Length Individual Proviral Sequencing assay to determine the distribution of latent replication-competent HIV-1 within memory CD4+T cell subsets.

Tue Oct 17 00:00:00 EDT 2017

Differential adipokine receptor expression on circulating leukocyte subsets in lean and obese children.

PloS one

Keustermans G,van der Heijden LB,Boer B,Scholman R,Nuboer R,Pasterkamp G,Prakken B,de Jager W,Kalkhoven E,Janse AJ,Schipper HS

47-0279 was used in Flow cytometry/Cell sorting to assess the expression of adiponectin and leptin receptors on circulating immune cells in obese children pre and post-lifestyle intervention compared to normal weight control children.

Mon Nov 20 00:00:00 EST 2017

HCV monoinfection and HIV/HCV coinfection enhance T-cell immune senescence in injecting drug users early during infection.

Immunity & ageing : I & A

Grady BP,Nanlohy NM,van Baarle D

47-0279 was used in Flow cytometry/Cell sorting to investigate the impact of HCV monoinfection and HIV/HCV coinfection on immune parameters associated with senescence in injecting drug users.

Fri Apr 01 00:00:00 EDT 2016

Cytomegalovirus Infection Leads to Development of High Frequencies of Cytotoxic Virus-Specific CD4+ T Cells Targeted to Vascular Endothelium.

PLoS pathogens

Pachnio A,Ciaurriz M,Begum J,Lal N,Zuo J,Beggs A,Moss P

47-0279 was used in Flow cytometry/Cell sorting to reveal the marked accumulation and unique phenotype of CMV-specific CD4+ T cells.

Thu Sep 01 00:00:00 EDT 2016

Site-specific differences in T cell frequencies and phenotypes in the blood and gut of HIV-uninfected and ART-treated HIV+ adults.

PloS one

Yukl SA,Shergill AK,Girling V,Li Q,Killian M,Epling L,Li P,Kaiser P,Haase A,Havlir DV,McQuaid K,Sinclair E,Wong JK

47-0279 was used in Flow cytometry/Cell sorting to study the effect of ART on T lymphocyte number and function in the blood, ileum, and rectum of HIV+ adults.

Wed Feb 24 00:00:00 EST 2016

Altered B cell balance, but unaffected B cell capacity to limit monocyte activation in anti-neutrophil cytoplasmic antibody-associated vasculitis in remission.

Rheumatology (Oxford, England)

Lepse N,Abdulahad WH,Rutgers A,Kallenberg CG,Stegeman CA,Heeringa P

47-0279 was used in Flow cytometry/Cell sorting to assess the frequency and function of Bregs in patients with ANCA-associated vasculitis.

Mon Sep 01 00:00:00 EDT 2014

Long-lived Plasmodium falciparum specific memory B cells in naturally exposed Swedish travelers.

European journal of immunology

Ndungu FM,Lundblom K,Rono J,Illingworth J,Eriksson S,Färnert A

Published figure using CD27 monoclonal antibody (Product # 47-0279-42) in Flow Cytometry

Fri Nov 01 00:00:00 EDT 2013

Chronic exposure to Plasmodium falciparum is associated with phenotypic evidence of B and T cell exhaustion.

Journal of immunology (Baltimore, Md. : 1950)

Illingworth J,Butler NS,Roetynck S,Mwacharo J,Pierce SK,Bejon P,Crompton PD,Marsh K,Ndungu FM

Published figure using CD27 monoclonal antibody (Product # 47-0279-42) in Flow Cytometry

Fri Feb 01 00:00:00 EST 2013

Alterations in peripheral blood B cell subsets and dynamics of B cell responses during human schistosomiasis.

PLoS neglected tropical diseases

Labuda LA,Ateba-Ngoa U,Feugap EN,Heeringa JJ,van der Vlugt LE,Pires RB,Mewono L,Kremsner PG,van Zelm MC,Adegnika AA,Yazdanbakhsh M,Smits HH

47-0279 was used in Flow cytometry/Cell sorting to characterise the B cell response to S. haematobium infection.

Wed Aug 28 00:00:00 EDT 2013

Schistosomes induce regulatory features in human and mouse CD1d(hi) B cells: inhibition of allergic inflammation by IL-10 and regulatory T cells.

PloS one

van der Vlugt LE,Labuda LA,Ozir-Fazalalikhan A,Lievers E,Gloudemans AK,Liu KY,Barr TA,Sparwasser T,Boon L,Ngoa UA,Feugap EN,Adegnika AA,Kremsner PG,Gray D,Yazdanbakhsh M,Smits HH

47-0279 was used in Flow cytometry/Cell sorting to investigate the effect of Schistosoma mansoni infection on allergic airway inflammation, showing it was inhibited by IL-10 produced by CD1d(hi) B cells.

Tue Jul 31 00:00:00 EDT 2012

Long-term immunologically competent human peripheral lymphoid tissue cultures in a 3D bioreactor.

Biotechnology and bioengineering

Kuzin I,Sun H,Moshkani S,Feng C,Mantalaris A,Wu JH,Bottaro A

Published figure using CD27 monoclonal antibody (Product # 47-0279-42) in Flow Cytometry

Wed Jun 01 00:00:00 EDT 2011

Bioinformatics

Protein Aliases: CD27; CD27 antigen; CD27L receptor; LPFS2; sCD27; soluble CD27; T cell activation antigen S152; T-cell activation antigen CD27; T14; TNFSF7; Tumor necrosis factor receptor superfamily member 7; tumor necrosis factor receptor superfamily, member 7

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Gene Aliases: CD27; S152; S152. LPFS2; T14; TNFRSF7; Tp55

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UniProt ID: (Human) P26842

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Entrez Gene ID: (Human) 939

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Function(s)

transmembrane signaling receptor activity tumor necrosis factor-activated receptor activity protein binding cysteine-type endopeptidase inhibitor activity involved in apoptotic process

Process(es)

inflammatory response immune response cell surface receptor signaling pathway brain development release of cytoplasmic sequestered NF-kappaB extrinsic apoptotic signaling pathway via death domain receptors immunoglobulin mediated immune response response to lipopolysaccharide tumor necrosis factor-mediated signaling pathway regulation of cell proliferation negative regulation of apoptotic process negative regulation of cysteine-type endopeptidase activity involved in apoptotic process positive regulation of MAPK cascade response to ethanol positive regulation of B cell differentiation positive regulation of JNK cascade negative regulation of T cell apoptotic process extrinsic apoptotic signaling pathway

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD27 Monoclonal Antibody (O323), APC-eFluor™ 780, eBioscience™

CD27 Antibody (47-0279-42) in Flow

Product Details

47-0279-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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