Invitrogen

CD27 Monoclonal Antibody (O323), PE-Cyanine5, eBioscience™

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CD27 Antibody (15-0279-42) in Flow

Staining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control PE-Cyanine5 (Product # 15-4714-81) (open histogram) or 0.25 µg of Anti-Human CD27 PE-Cyanine5 (filled histogram).Cells in the lymphocyte gate were used for analysis.

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Product Details

15-0279-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.25 µg)/test

View 21 publications 21 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Rhesus monkey

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE-Cyanine5, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

O323

Conjugate

PE-Cyanine5 PE-Cyanine5 PE-Cyanine5

Excitation/Emission Max

568/666 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10717249

Product Specific Information

Description: The O323 monoclonal antibody reacts with human CD27, a lymphocyte-specific member of the TNFR superfamily. CD27 is expressed by a subset of thymocytes and virtually all mature T cells and is upregulated upon T-cell stimulation. CD27 binds to CD70, and through this interaction, plays an important role in T cell-B cell interaction.

Applications Reported: This O323 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This O323 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 667 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD27 is a 50 kDa member of the tumor necrosis factor (TNF) receptor superfamily that includes CD40 and CD30. The TNF superfamily members are known for the regulation of cell proliferation and death. In contrast to the expression of other TNFR/TNF family members, expression of CD27 and its ligand CD70 is predominantly confined to lymphocytes. High expression levels of CD27 appear to be dependent on proper ligation of antigen receptors. CD70 expression requires additional co-stimulatory and/or pro-inflammatory signals. CD27 is expressed as a disulfide-linked homodimer on mature thymocytes, peripheral blood T cells and a subpopulation of B cells. Activation of T cells via TCR-CD3 complex results in upregulation of CD27 expression on the plasma membrane as well as in the release of its soluble 28-32 kDa form, sCD27, detected in the plasma, urine or spinal fluid. Soluble CD27 is an important prognostic marker of acute and chronic B cell malignancies. RgpA, a cystein proteinase, although activating T cells through the protease-activated receptors (PARs), degradates CD27 and counteracts T cell activation mediated by CD27 and its ligand CD70. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor. Diseases associated with CD27 dysfunction include Lymphoproliferative Syndrome 2 and Autosomal Recessive Lymphoproliferative Syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

B-cell targeting with anti-CD38 daratumumab: implications for differentiation and memory responses.

Life science alliance

Verhoeven D,Grinwis L,Marsman C,Jansen MH,Van Leeuwen EM,Kuijpers TW

Published figure using CD27 monoclonal antibody (Product # 15-0279-42) in Flow Cytometry

Fri Sep 01 00:00:00 EDT 2023

Germinal center responses to SARS-CoV-2 mRNA vaccines in healthy and immunocompromised individuals.

Cell

Lederer K,Bettini E,Parvathaneni K,Painter MM,Agarwal D,Lundgreen KA,Weirick M,Muralidharan K,Castaño D,Goel RR,Xu X,Drapeau EM,Gouma S,Ort JT,Awofolaju M,Greenplate AR,Le Coz C,Romberg N,Trofe-Clark J,Malat G,Jones L,Rosen M,Weiskopf D,Sette A,Besharatian B,Kaminiski M,Hensley SE,Bates P,Wherry EJ,Naji A,Bhoj V,Locci M

15-0279-42 was used in Flow Cytometry to study the germinal center responses elicited by SARS-CoV-2 mRNA vaccines in draining lymph nodes of healthy donors and kidney transplant recipients and assess their connection to humoral and memory B cell responses.

Thu Mar 17 00:00:00 EDT 2022

Oxygen level is a critical regulator of human B cell differentiation and IgG class switch recombination.

Frontiers in immunology

Koers J,Marsman C,Steuten J,Tol S,Derksen NIL,Ten Brinke A,van Ham SM,Rispens T

Published figure using CD27 monoclonal antibody (Product # 15-0279-42) in Flow Cytometry

Tue Jan 03 00:00:00 EST 2023

A combined fine needle aspiration and spectral flow cytometry approach to assess human germinal center responses to SARS-CoV-2 vaccination.

STAR protocols

Bettini E,Lederer K,Sharpe H,Kaminski M,Jones L,Locci M

15-0279-42 was used in Flow cytometry/Cell sorting to describe a procedure for the acquisition of human lymph node samples via an ultrasound-guided fine needle aspiration-based approach.

Fri Dec 16 00:00:00 EST 2022

Analysis of KSHV B lymphocyte lineage tropism in human tonsil reveals efficient infection of CD138+ plasma cells.

PLoS pathogens

Aalam F,Nabiee R,Castano JR,Totonchy J

15-0279 was used in Flow cytometry/Cell sorting to identify CD138+ plasma cells as a highly targeted cell type for de novo Kaposi Sarcoma Herpesvirus infection in human tonsils.

Thu Oct 01 00:00:00 EDT 2020

Expression of CD27 and CD28 on γδ T cells from the peripheral blood of patients with allergic rhinitis.

Experimental and therapeutic medicine

Wang Q,Sun Q,Chen Q,Li H,Liu D

Published figure using CD27 monoclonal antibody (Product # 15-0279-42) in Flow Cytometry

Tue Dec 01 00:00:00 EST 2020

Developing Human Skin Contains Lymphocytes Demonstrating a Memory Signature.

Cell reports. Medicine

Dhariwala MO,Karthikeyan D,Vasquez KS,Farhat S,Weckel A,Taravati K,Leitner EG,Clancy S,Pauli M,Piper ML,Cohen JN,Ashouri JF,Lowe MM,Rosenblum MD,Scharschmidt TC

Published figure using CD27 monoclonal antibody (Product # 15-0279-42) in Flow Cytometry

Tue Nov 17 00:00:00 EST 2020

T-bet+ Memory B Cells Link to Local Cross-Reactive IgG upon Human Rhinovirus Infection.

Cell reports

Eccles JD,Turner RB,Kirk NA,Muehling LM,Borish L,Steinke JW,Payne SC,Wright PW,Thacker D,Lahtinen SJ,Lehtinen MJ,Heymann PW,Woodfolk JA

15-0279 was used in Flow cytometry/Cell sorting to identify T-bet+ B cells binding both viruses that account for ~5% of CXCR5- memory B cells.

Tue Jan 14 00:00:00 EST 2020

Circulating Exosomes Inhibit B Cell Proliferation and Activity.

Cancers

Schroeder JC,Puntigam L,Hofmann L,Jeske SS,Beccard IJ,Doescher J,Laban S,Hoffmann TK,Brunner C,Theodoraki MN,Schuler PJ

15-0279 was used in Flow cytometry/Cell sorting to isolate and analyse peripheral B cells by flow cytometry to understand the effects of exosomes on B cells.

Wed Jul 29 00:00:00 EDT 2020

More than one antibody of individual B cells revealed by single-cell immune profiling.

Cell discovery

Shi Z,Zhang Q,Yan H,Yang Y,Wang P,Zhang Y,Deng Z,Yu M,Zhou W,Wang Q,Yang X,Mo X,Zhang C,Huang J,Dai H,Sun B,Zhao Y,Zhang L,Yang YG,Qiu X

Published figure using CD27 monoclonal antibody (Product # 15-0279-42) in Flow Cytometry

Fri Oct 20 00:00:00 EDT 2023

Perturbed CD8⁺ T cell immunity across universal influenza epitopes in the elderly.

Journal of leukocyte biology

Nguyen THO,Sant S,Bird NL,Grant EJ,Clemens EB,Koutsakos M,Valkenburg SA,Gras S,Lappas M,Jaworowski A,Crowe J,Loh L,Kedzierska K

Published figure using CD27 monoclonal antibody (Product # 15-0279-42) in Flow Cytometry

Thu Feb 01 00:00:00 EST 2018

Frequency of Circulating CD4⁺Ki67⁺HLA-DR^- T Regulatory Cells Prior to Treatment for Multidrug Resistant Tuberculosis Can Differentiate the Severity of Disease and Predict Time to Culture Conversion.

Frontiers in immunology

Ferrian S,Ross M,Conradie F,Vally Omar S,Ismail N,Little F,Kaplan G,Fallows D,Gray CM

15-0279 was used in Flow cytometry/Cell sorting to reveal that the frequency of a highly defined Mtb-stimulated blood Treg cell population at baseline can discriminate MDR-TB diease severity and predict time to culture clearance.

Wed Oct 02 00:00:00 EDT 2019

Maintenance of the EBV-specific CD8⁺ TCRαβ repertoire in immunosuppressed lung transplant recipients.

Immunology and cell biology

Nguyen TH,Bird NL,Grant EJ,Miles JJ,Thomas PG,Kotsimbos TC,Mifsud NA,Kedzierska K

Published figure using CD27 monoclonal antibody (Product # 15-0279-42) in Flow Cytometry

Sun Jan 01 00:00:00 EST 2017

Abnormal phenotypic features of IgM+B cell subsets in patients with chronic hepatitis C virus infection.

Experimental and therapeutic medicine

Kong F,Feng B,Zhang H,Rao H,Wang J,Cong X,Wei L

Published figure using CD27 monoclonal antibody (Product # 15-0279-42) in Flow Cytometry

Tue Aug 01 00:00:00 EDT 2017

Antigen receptor-redirected T cells derived from hematopoietic precursor cells lack expression of the endogenous TCR/CD3 receptor and exhibit specific antitumor capacities.

Oncoimmunology

Van Caeneghem Y,De Munter S,Tieppo P,Goetgeluk G,Weening K,Verstichel G,Bonte S,Taghon T,Leclercq G,Kerre T,Debets R,Vermijlen D,Abken H,Vandekerckhove B

Published figure using CD27 monoclonal antibody (Product # 15-0279-42) in Flow Cytometry

Tue Jan 12 00:00:00 EST 2021

Reevaluation of immune activation in the era of cART and an aging HIV-infected population.

JCI insight

de Armas LR,Pallikkuth S,George V,Rinaldi S,Pahwa R,Arheart KL,Pahwa S

15-0279 was used in Flow cytometry/Cell sorting to assess the relationship between markers of immune activation and inflammation during biological ageing in HIV-infected and -uninfected populations.

Thu Oct 19 00:00:00 EDT 2017

Strong HCV NS3/4a, NS4b, NS5a, NS5b-specific cellular immune responses induced in Rhesus macaques by a novel HCV genotype 1a/1b consensus DNA vaccine.

Human vaccines & immunotherapeutics

Latimer B,Toporovski R,Yan J,Pankhong P,Morrow MP,Khan AS,Sardesai NY,Welles SL,Jacobson JM,Weiner DB,Kutzler MA

15-0279 was used in Flow cytometry/Cell sorting to show that macaque models are useful to depict key anti-HCV nonstructural antigenic cellular immune responses to support vaccine development.

Fri Jul 10 00:00:00 EDT 2015

A subset of circulating blood mycobacteria-specific CD4 T cells can predict the time to Mycobacterium tuberculosis sputum culture conversion.

PloS one

Riou C,Gray CM,Lugongolo M,Gwala T,Kiravu A,Deniso P,Stewart-Isherwood L,Omar SV,Grobusch MP,Coetzee G,Conradie F,Ismail N,Kaplan G,Fallows D

15-0279 was used in Flow cytometry/Cell sorting to investigate 18 HIV-negative patients with MDR-TB for M. tuberculosis- and PPD-specific CD4 T cell responses and followed them over 6 months of drug therapy.

Tue Apr 07 00:00:00 EDT 2015

Long-lived Plasmodium falciparum specific memory B cells in naturally exposed Swedish travelers.

European journal of immunology

Ndungu FM,Lundblom K,Rono J,Illingworth J,Eriksson S,Färnert A

Published figure using CD27 monoclonal antibody (Product # 15-0279-42) in Flow Cytometry

Fri Nov 01 00:00:00 EDT 2013

Chronic exposure to Plasmodium falciparum is associated with phenotypic evidence of B and T cell exhaustion.

Journal of immunology (Baltimore, Md. : 1950)

Illingworth J,Butler NS,Roetynck S,Mwacharo J,Pierce SK,Bejon P,Crompton PD,Marsh K,Ndungu FM

Published figure using CD27 monoclonal antibody (Product # 15-0279-42) in Flow Cytometry

Fri Feb 01 00:00:00 EST 2013

Long-term immunologically competent human peripheral lymphoid tissue cultures in a 3D bioreactor.

Biotechnology and bioengineering

Kuzin I,Sun H,Moshkani S,Feng C,Mantalaris A,Wu JH,Bottaro A

Published figure using CD27 monoclonal antibody (Product # 15-0279-42) in Flow Cytometry

Wed Jun 01 00:00:00 EDT 2011

Bioinformatics

Protein Aliases: CD27; CD27 antigen; CD27L receptor; LPFS2; sCD27; soluble CD27; T cell activation antigen S152; T-cell activation antigen CD27; T14; TNFSF7; Tumor necrosis factor receptor superfamily member 7; tumor necrosis factor receptor superfamily, member 7

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Gene Aliases: CD27; S152; S152. LPFS2; T14; TNFRSF7; Tp55

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UniProt ID: (Human) P26842

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Entrez Gene ID: (Human) 939

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Function(s)

transmembrane signaling receptor activity tumor necrosis factor-activated receptor activity protein binding cysteine-type endopeptidase inhibitor activity involved in apoptotic process

Process(es)

inflammatory response immune response cell surface receptor signaling pathway brain development release of cytoplasmic sequestered NF-kappaB extrinsic apoptotic signaling pathway via death domain receptors immunoglobulin mediated immune response response to lipopolysaccharide tumor necrosis factor-mediated signaling pathway regulation of cell proliferation negative regulation of apoptotic process negative regulation of cysteine-type endopeptidase activity involved in apoptotic process positive regulation of MAPK cascade response to ethanol positive regulation of B cell differentiation positive regulation of JNK cascade negative regulation of T cell apoptotic process extrinsic apoptotic signaling pathway

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD27 Monoclonal Antibody (O323), PE-Cyanine5, eBioscience™

CD27 Antibody (15-0279-42) in Flow

Product Details

15-0279-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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