Invitrogen

CD197 (CCR7) Monoclonal Antibody (3D12), eBioscience™

14 Published Figures

26 References

View all (60) CCR7 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Published Figures (14)

CD197 (CCR7) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 14

CD197 (CCR7) Antibody (14-1979-82) in Flow

Cancer communications (London, England) 2023 - 5 FIGURE The PTG-T16R-scFV-CAR-T cells enhanced antitumor immunity in vivo. (A) Schematic diagram of humanized NSG mice cholangiocarcinoma model. (B-E) After subcutaneously inoculating humanized NSG mice with TFK-1 cells for 7 days, the mice were transfused with the PTG-T16R-EGFR-CAR-T cells, EGFR-CAR-T cells, and GP120-CAR-T cells via the tail vein. 28 days later, the mice were euthanized. Tumor size was m... View More

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Product Details

14-1979-82

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

Assay-Dependent

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

0.5 µg/test

View 24 publications 24 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse, Non-human primate

Host/Isotype

Rat / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

3D12

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_842734

Product Specific Information

Description: The 3D12 monoclonal antibody reacts with human CCR7, also known as EBI-1 and CD197. CCR7 is a member of the G-protein-coupled chemokine receptor family with seven membrane-spanning domains and functions as a receptor for 6Ckine/SLC (secondary lymphoid-tissue chemokine), CCL19 and CCL21. CCR7 has been shown to be internalized via clathrin-coated pits and the majority recycled back to the plasma membrane. CCR7 is expressed on T cells and can be used to distinguish populations of naive from central and effector memory T cells. CCR7 has been shown to play a role in migration of memory T cells to inflamed tissue. Expression of CCR7 is also found on DC's. During DC maturation CCR7 expression increases and is thought to be involved in a variety of functions: chemotaxis to the lymph node, cellular architecture, rate of endocytosis, survival and maturation. Expression of CCR7 on the cell surface can be down regulated upon ligand binding.

Applications Reported: This 3D12 antibody has been reported for use in flow cytometric analysis and immunohistology staining of paraffin embedded tissue sections.

Applications Tested: This 3D12 antibody has been tested by flow cytometric analysis of human peripheral blood cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

It is recommended that the staining incubation time be increased to at least 45 minutes at 2-8°C for optimal staining.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CCR7 is a member of the G protein coupled receptor family, which is a subfamily of chemokines. CCR7 was identified to be induced by the Epstein Barr virus (EBV), and is thought to be a mediator of EBV effects on B lymphocytes. CCR7 has been reported to be expressed in blood, bone marrow, lymph node, and intestine. CCR7 is particularly expressed in lymphoid tissues and in activated B and T lymphocytes and has been shown to control the migration of memory T cells to inflamed tissues, as well as stimulate dendritic cell maturation. The chemokine (C-C motif) ligand 19 (CCL19/ECL) has been reported to be a specific ligand of this receptor. ESTs have been isolated from blood, embryo, lymph node, and thymus libraries. Receptors for the C - C chemokine family include CCR 1, CCR 2A, CCR 3, CCR 4, CCR 5 and the Duffy blood group antigen. The C-C receptors are important in the function of T cell chemotaxis and migration of phagocytic cells to sites of inflammation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Enhancement of CAR-T cell activity against cholangiocarcinoma by simultaneous knockdown of six inhibitory membrane proteins.

Cancer communications (London, England)

Qiao Y,Chen J,Wang X,Yan S,Tan J,Xia B,Chen Y,Lin K,Zou F,Liu B,He X,Zhang Y,Zhang X,Zhang H,Wu X,Lu L

Published figure using CD197 (CCR7) monoclonal antibody (Product # 14-1979-82) in Flow Cytometry

Sat Jul 01 00:00:00 EDT 2023

Neuroimmune Consequences of eIF4E Phosphorylation on Chemotherapy-Induced Peripheral Neuropathy.

Frontiers in immunology

Agalave NM,Mody PH,Szabo-Pardi TA,Jeong HS,Burton MD

Published figure using CD197 (CCR7) monoclonal antibody (Product # 14-1979-82) in Flow Cytometry

Mon Sep 20 00:00:00 EDT 2021

Alteration of the Immune Microenvironment in HBsAg and HBeAg Dual-Positive Pregnant Women Presenting a High HBV Viral Load.

Journal of inflammation research

Gao F,Wang H,Li X,Guo F,Yuan Y,Wang X,Zhang Y,Bai G

Published figure using CD197 (CCR7) monoclonal antibody (Product # 14-1979-82) in Flow Cytometry

Thu Apr 28 00:00:00 EDT 2022

Application of the chemokine-chemokine receptor axis increases the tumor-targeted migration ability of cytokine-induced killer cells in patients with colorectal cancer.

Oncology letters

Zou Y,Liang J,Li D,Fang J,Wang L,Wang J,Zhang J,Guo Q,Yan X,Tang H

Published figure using CD197 (CCR7) monoclonal antibody (Product # 14-1979-82) in Flow Cytometry

Wed Jul 01 00:00:00 EDT 2020

Development of CAR-T cell therapy for B-ALL using a point-of-care approach.

Oncoimmunology

de Macedo Abdo L,Barros LRC,Saldanha Viegas M,Vieira Codeço Marques L,de Sousa Ferreira P,Chicaybam L,Bonamino MH

Published figure using CD197 (CCR7) monoclonal antibody (Product # 14-1979-82) in Flow Cytometry

Wed Jul 14 00:00:00 EDT 2021

The Diagnostic Value of Chemokine/Chemokine Receptor Pairs in Hepatocellular Carcinoma and Colorectal Liver Metastasis.

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society

Jiao X,Shu G,Liu H,Zhang Q,Ma Z,Ren C,Guo H,Shi J,Liu J,Zhang C,Wang Y,Gao Y

14-1979 was used in Immunohistochemistry on paraffin embedded tissues to investigate the roles of chemokines and their receptors in the progression of primary and metastatic malignant liver tumors and their prognosis.

Wed May 01 00:00:00 EDT 2019

Intracellular delivery of mRNA to human primary T cells with microfluidic vortex shedding.

Scientific reports

Jarrell JA,Twite AA,Lau KHWJ,Kashani MN,Lievano AA,Acevedo J,Priest C,Nieva J,Gottlieb D,Pawell RS

Published figure using CD197 (CCR7) monoclonal antibody (Product # 14-1979-82) in Flow Cytometry

Fri Mar 01 00:00:00 EST 2019

Boiling Histotripsy-induced Partial Mechanical Ablation Modulates Tumour Microenvironment by Promoting Immunogenic Cell Death of Cancers.

Scientific reports

Pahk KJ,Shin CH,Bae IY,Yang Y,Kim SH,Pahk K,Kim H,Oh SJ

14-1979-82 was used in Flow Cytometry to suggest that boiling histotripsy could be a potential therapeutic approach for not only mechanically destroying solid tumours (e.g., breast cancer) but also promoting immunogenic cell death via TNF-induced necrosis to trigger antitumour immunity.

Fri Jun 21 00:00:00 EDT 2019

The Calcineurin Inhibitor Tacrolimus Specifically Suppresses Human T Follicular Helper Cells.

Frontiers in immunology

Wallin EF,Hill DL,Linterman MA,Wood KJ

14-1979 was used in Flow cytometry/Cell sorting to study the impact of the immunosuppressive agent Tacrolimuson T follicular helper cells differentiationand function in transplant recipients.

Fri Aug 16 00:00:00 EDT 2019

Characteristic patterns of HLA presentation and T cell differentiation in adult-onset Still's disease.

International journal of immunopathology and pharmacology

Jung JY,Choi B,Sayeed HM,Suh CH,Kim YW,Kim HA,Sohn S

Published figure using CD197 (CCR7) monoclonal antibody (Product # 14-1979-82) in Flow Cytometry

Tue Dec 18 00:00:00 EST 2018

Biochanin a Enhances the Defense Against Salmonella enterica Infection Through AMPK/ULK1/mTOR-Mediated Autophagy and Extracellular Traps and Reversing SPI-1-Dependent Macrophage (MΦ) M2 Polarization.

Frontiers in cellular and infection microbiology

Zhao X,Tang X,Guo N,An Y,Chen X,Shi C,Wang C,Li Y,Li S,Xu H,Liu M,Wang Y,Yu L

Published figure using CD197 (CCR7) monoclonal antibody (Product # 14-1979-82) in Flow Cytometry

Fri Aug 30 00:00:00 EDT 2019

Oral Supplementation with Baker's Yeast Beta Glucan Is Associated with Altered Monocytes, T Cells and Cytokines following a Bout of Strenuous Exercise.

Frontiers in physiology

McFarlin BK,Venable AS,Carpenter KC,Henning AL,Ogenstad S

Published figure using CD197 (CCR7) monoclonal antibody (Product # 14-1979-82) in Flow Cytometry

Wed Sep 30 00:00:00 EDT 2020

Monocyte differentiation and macrophage priming are regulated differentially by pentraxins and their ligands.

BMC immunology

Pilling D,Galvis-Carvajal E,Karhadkar TR,Cox N,Gomer RH

14-1979 was used in Immunocytochemistry to suggest that the presence of pentraxins and their ligands regulate macrophage differentiation in the blood and tissues.

Thu Jun 15 00:00:00 EDT 2017

Efficient generation of antigen-specific CTLs by the BAFF-activated human B Lymphocytes as APCs: a novel approach for immunotherapy.

Oncotarget

Yiwen Z,Shilin G,Yingshi C,Lishi S,Baohong L,Chao L,Linghua L,Ting P,Hui Z

14-1979 was used in Flow cytometry/Cell sorting to expand human B-lymphocytes on a large scale while maintaining their antigen-presenting ability by using both CD40L and B-cell activating factor (BAFF).

Tue Nov 22 00:00:00 EST 2016

FOXP3+ Regulatory T Cells in Hepatic Fibrosis and Splenomegaly Caused by Schistosoma japonicum: The Spleen May Be a Major Source of Tregs in Subjects with Splenomegaly.

PLoS neglected tropical diseases

Romano A,Hou X,Sertorio M,Dessein H,Cabantous S,Oliveira P,Li J,Oyegue S,Arnaud V,Luo X,Daujat-Chavanieu M,Mariani O,Sastre X,Dombey AM,He H,Li Y,Dessein A

14-1979 was used in Flow cytometry/Cell sorting to investigate whether FOXP3+ Tregs play a role in human schistosomiasis.

Fri Jan 01 00:00:00 EST 2016

Brief Report: IFIH1 Mutation Causes Systemic Lupus Erythematosus With Selective IgA Deficiency.

Arthritis & rheumatology (Hoboken, N.J.)

Van Eyck L,De Somer L,Pombal D,Bornschein S,Frans G,Humblet-Baron S,Moens L,de Zegher F,Bossuyt X,Wouters C,Liston A

14-1979 was used in Flow cytometry/Cell sorting to determine the underlying genetic defect responsible for severe early onset and refractory systemic lupus erythematosus.

Mon Jun 01 00:00:00 EDT 2015

Prevailing role of contact guidance in intrastromal T-cell trapping in human pancreatic cancer.

Clinical cancer research : an official journal of the American Association for Cancer Research

Hartmann N,Giese NA,Giese T,Poschke I,Offringa R,Werner J,Ryschich E

14-1979 was used in Flow cytometry/Cell sorting to analyse how chemokines and extracellular matrix collagen interact in mediating T-cell infiltration in pancreatic ductal adenocarcinoma.

Tue Jul 01 00:00:00 EDT 2014

Peptide-induced immune regulation by a promiscuous and immunodominant CD4T-cell epitope of Timothy grass pollen: a role of Cbl-b and Itch in regulation.

Thorax

Till SJ,Raynsford EJ,Reynolds CJ,Quigley KJ,Grzybowska-Kowalczyk A,Saggar LR,Goldstone A,Maillere B,Kwok WW,Altmann DM,Durham SR,Boyton RJ

14-1979 was used in Flow cytometry/Cell sorting to identify human leukocyte antigen-DR1-restricted epitopes from the Timothy grass pollen allergen, Phleum pratense, and characterise T-cell immune regulation following intranasal administration of a single, immunodominant epitope.

Tue Apr 01 00:00:00 EDT 2014

Innate immune responses and rapid control of inflammation in African green monkeys treated or not with interferon-alpha during primary SIVagm infection.

PLoS pathogens

Jacquelin B,Petitjean G,Kunkel D,Liovat AS,Jochems SP,Rogers KA,Ploquin MJ,Madec Y,Barré-Sinoussi F,Dereuddre-Bosquet N,Lebon P,Le Grand R,Villinger F,Müller-Trutwin M

14-1979 was used in Flow cytometry/Cell sorting to investigate the role of innate immune cells and IFN-α in the control of chronic immune activation, showing that control is independent of IFN-α levels in SIV infection.

Tue Jul 01 00:00:00 EDT 2014

Secondary lymphoid organ homing phenotype of human myeloid dendritic cells disrupted by an intracellular oral pathogen.

Infection and immunity

Miles B,Zakhary I,El-Awady A,Scisci E,Carrion J,O'Neill JC,Rawlings A,Stern JK,Susin C,Cutler CW

Published figure using CD197 (CCR7) monoclonal antibody (Product # 14-1979-82) in Flow Cytometry

Wed Jan 01 00:00:00 EST 2014

Kinetics of myeloid dendritic cell trafficking and activation: impact on progressive, nonprogressive and controlled SIV infections.

PLoS pathogens

Wijewardana V,Kristoff J,Xu C,Ma D,Haret-Richter G,Stock JL,Policicchio BB,Mobley AD,Nusbaum R,Aamer H,Trichel A,Ribeiro RM,Apetrei C,Pandrea I

14-1979 was used in Flow cytometry/Cell sorting to provide a mechanistic basis for the role of myeloid dendritic cells in the pathogenesis of AIDS.

Mon May 26 00:00:00 EDT 2014

Helios+ and Helios- cells coexist within the natural FOXP3+ T regulatory cell subset in humans.

Journal of immunology (Baltimore, Md. : 1950)

Himmel ME,MacDonald KG,Garcia RV,Steiner TS,Levings MK

14-1979 was used in Flow cytometry/Cell sorting to investigate the link between Helios expression and natural regulatory FOXP3+ T cell subsets from different origins in humans.

Fri Mar 01 00:00:00 EST 2013

IL-15 transpresentation promotes both human T-cell reconstitution and T-cell-dependent antibody responses in vivo.

Proceedings of the National Academy of Sciences of the United States of America

Huntington ND,Alves NL,Legrand N,Lim A,Strick-Marchand H,Mention JJ,Plet A,Weijer K,Jacques Y,Becker PD,Guzman C,Soussan P,Kremsdorf D,Spits H,Di Santo JP

14-1979 was used in Flow cytometry/Cell sorting to investigate the role of transpresented IL-15 on human T-cell development and homeostasis.

Tue Apr 12 00:00:00 EDT 2011

The effect of pregnancy on the uterine NK cell KIR repertoire.

European journal of immunology

Male V,Sharkey A,Masters L,Kennedy PR,Farrell LE,Moffett A

14-1979 was used in Flow cytometry/Cell sorting to examine the natural killer cell population in the decidua during pregnancy.

Sat Oct 01 00:00:00 EDT 2011

CD137 ligand signaling induces human monocyte to dendritic cell differentiation.

European journal of immunology

Kwajah M M S,Schwarz H

14-1979 was used in Flow cytometry/Cell sorting to deduce that peripheral monocytes can be differentiated into mature inflammatory dendritic cells by the single factor CD137.

Thu Jul 01 00:00:00 EDT 2010

Role of TLX1 in T-cell acute lymphoblastic leukaemia pathogenesis.

British journal of haematology

Riz I,Hawley TS,Johnston H,Hawley RG

Published figure using CD197 (CCR7) monoclonal antibody (Product # 14-1979-82) in Flow Cytometry

Wed Apr 01 00:00:00 EDT 2009

Role of TLX1 in T-cell acute lymphoblastic leukaemia pathogenesis.

British journal of haematology

Riz I,Hawley TS,Johnston H,Hawley RG

Published figure using CD197 (CCR7) monoclonal antibody (Product # 14-1979-82) in Flow Cytometry

Wed Apr 01 00:00:00 EDT 2009

Bioinformatics

Protein Aliases: BLR2; Bukitt's lymphoma receptor 2; C-C chemokine receptor type 7; C-C CKR-7; CC chemokine receptor 7; CD197; CDw197; chemokine (C-C motif) receptor 7; EBI1; EBV-induced G protein-coupled receptor 1; Epstein-Barr virus induced gene 1; Epstein-Barr virus-induced G-protein coupled receptor 1; lymphocyte-specific G protein-coupled peptide receptor; MIP-3 beta receptor

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Gene Aliases: BLR2; CC-CKR-7; CCR-7; CCR7; CD197; CDw197; CMKBR7; EBI1; EVI1

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UniProt ID: (Human) P32248

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Entrez Gene ID: (Human) 1236

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Function(s)

G-protein coupled receptor activity C-C chemokine receptor activity chemokine (C-C motif) ligand 19 binding chemokine (C-C motif) ligand 21 binding C-C motif chemokine 19 receptor activity C-C motif chemokine 21 receptor activity

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD197 (CCR7) Monoclonal Antibody (3D12), eBioscience™

CD197 (CCR7) Antibody (14-1979-82) in Flow

Product Details

14-1979-82

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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