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Invitrogen
CD197 (CCR7) Monoclonal Antibody (3D12), NovaFluor™ Yellow 700, eBioscience™
FIGURE: 1 / 2
CD197 (CCR7) Antibody (H085T03Y06-A) in Flow
Normal human peripheral blood cells were unstained (left) or stained with CD197 (CCR7) Monoclonal Antibody, NovaFluor Yellow 700 (right). All cells were co-stained with CD45RA Monoclonal Antibody, eFluor 450 (Product # 48-0458-42). Total viable cells in the lymphocyte gate were used for analysis, as determined by LIVE/DEAD Blue (Product # L34962). Data was acquired on a 5-laser Cytek Aurora and unmixed with autofluorescence extraction.
Product Details
H085T03Y06-A
Product Specifications
Species Reactivity
Human
Host/Isotype
Rat
/ IgG2a, kappa
Class
Monoclonal
Type
Antibody
Clone
3D12
Conjugate
Excitation/Emission Max
551/701 nm
View spectra
Form
Liquid
Concentration
0.6 µg/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with BSA
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Product Specific Information
Description: The 3D12 monoclonal antibody reacts with human CCR7, also known as EBI-1 and CD197. CCR7 is a member of the G-protein-coupled chemokine receptor family with seven membrane-spanning domains and functions as a receptor for 6Ckine/SLC (secondary lymphoid-tissue chemokine), CCL19 and CCL21. CCR7 has been shown to be internalized via clathrin-coated pits and the majority recycled back to the plasma membrane. CCR7 is expressed on T cells and can be used to distinguish populations of naive from central and effector memory T cells. CCR7 has been shown to play a role in migration of memory T cells to inflamed tissue. Expression of CCR7 is also found on DC's. During DC maturation CCR7 expression increases and is thought to be involved in a variety of functions: chemotaxis to the lymph node, cellular architecture, rate of endocytosis, survival and maturation. Expression of CCR7 on the cell surface can be down regulated upon ligand binding.
Applications Reported: This 3D12 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This 3D12 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.6 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
It is recommended that the staining incubation time be increased to at least 45 minutes at 2-8°C for optimal staining.
Each product contains 1 vial of NovaFluor conjugate and 1 vial of CellBlox Plus Blocking Buffer .
NovaFluor dyes are not compatible with DNA intercalating viability dyes. Do not use viability dyes such as propidium iodide, 7-actinomycin D (7-AAD) and DAPI. Invitrogen LIVE/DEAD Fixable Dead Cell stains are recommended for use with NovaFluor dyes.
This NovaFluor conjugate has been updated to ship with CellBlox Plus Blocking Buffer (Cat. No. (C001T06F01)). This buffer contains formulation improvements over CellBlox. CellBlox Plus Blocking Buffer is required for optimal staining with NovaFluor conjugates and should be used in all experiments where NovaFluor conjugates are used. Whenever possible, we recommend adding CellBlox Plus Blocking Buffer to antibody cocktails/master mixes prior to combining with cells. Add 5 µL per sample (regardless of the number of NovaFluors in your panel) to use the antibody cocktail as intended. For single-color controls, use 5 µL of CellBlox Blocking Buffer per 100 µL of cell sample containing 10^3 to 10^8 cells.
NovaFluor conjugates are based on Phiton™ technology utilizing novel nucleic acid dye structures that allow for engineered fluorescent signatures with consideration for spillover and spread impacts. Learn more
Excitation: 552 nm; Emission: 700 nm; Laser: 561 nm (Yellow) Laser
Target Information
CCR7 is a member of the G protein coupled receptor family, which is a subfamily of chemokines. CCR7 was identified to be induced by the Epstein Barr virus (EBV), and is thought to be a mediator of EBV effects on B lymphocytes. CCR7 has been reported to be expressed in blood, bone marrow, lymph node, and intestine. CCR7 is particularly expressed in lymphoid tissues and in activated B and T lymphocytes and has been shown to control the migration of memory T cells to inflamed tissues, as well as stimulate dendritic cell maturation. The chemokine (C-C motif) ligand 19 (CCL19/ECL) has been reported to be a specific ligand of this receptor. ESTs have been isolated from blood, embryo, lymph node, and thymus libraries. Receptors for the C - C chemokine family include CCR 1, CCR 2A, CCR 3, CCR 4, CCR 5 and the Duffy blood group antigen. The C-C receptors are important in the function of T cell chemotaxis and migration of phagocytic cells to sites of inflammation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: BLR2; Bukitt's lymphoma receptor 2; C-C chemokine receptor type 7; C-C CKR-7; CC chemokine receptor 7; CD197; CDw197; chemokine (C-C motif) receptor 7; EBI1; EBV-induced G protein-coupled receptor 1; Epstein-Barr virus induced gene 1; Epstein-Barr virus-induced G-protein coupled receptor 1; lymphocyte-specific G protein-coupled peptide receptor; MIP-3 beta receptor
Gene Aliases: BLR2; CC-CKR-7; CCR-7; CCR7; CD197; CDw197; CMKBR7; EBI1; EVI1
UniProt ID: (Human) P32248
Entrez Gene ID: (Human) 1236
establishment of T cell polarity
positive regulation of cell-matrix adhesion
dendritic cell chemotaxis
myeloid dendritic cell chemotaxis
positive regulation of dendritic cell antigen processing and presentation
positive regulation of hypersensitivity
positive regulation of humoral immune response
inflammatory response
immune response
G-protein coupled receptor signaling pathway
positive regulation of cytosolic calcium ion concentration
positive regulation of actin filament polymerization
positive regulation of pseudopodium assembly
ruffle organization
response to lipopolysaccharide
regulation of interferon-gamma production
positive regulation of interleukin-12 production
response to prostaglandin E
chemokine (C-C motif) ligand 19 signaling pathway
chemokine (C-C motif) ligand 21 signaling pathway
positive regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of phosphatidylinositol 3-kinase activity
negative thymic T cell selection
positive regulation of cell adhesion
positive regulation of protein kinase activity
positive regulation of JNK cascade
homeostasis of number of cells
regulation of interleukin-1 beta secretion
positive regulation of T cell receptor signaling pathway
release of sequestered calcium ion into cytosol
positive regulation of filopodium assembly
positive regulation of protein kinase B signaling
positive regulation of ERK1 and ERK2 cascade
cellular response to cytokine stimulus
response to nitric oxide
interleukin-12 secretion
positive regulation of neutrophil chemotaxis
activation of GTPase activity
lymphocyte migration into lymph node
mature conventional dendritic cell differentiation
negative regulation of leukocyte apoptotic process
positive regulation of cell motility
positive regulation of dendritic cell chemotaxis
positive regulation of immunological synapse formation
positive regulation of T cell costimulation
positive regulation of glycoprotein biosynthetic process involved in immunological synapse formation
regulation of dendritic cell dendrite assembly
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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