Invitrogen

CD197 (CCR7) Monoclonal Antibody (3D12), APC, eBioscience™

10 Published Figures

21 References

View all (60) CCR7 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (10)

CD197 (CCR7) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 11

CD197 (CCR7) Antibody (17-1979-42) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD45RA FITC (Product # 11-0458-42) and Rat IgG2a K Isotype Control APC (Product # 17-4321-81) (left) or Anti-Human CD197 (CCR7) APC (right). Cells in the lymphocyte gate were used for analysis.

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Product Details

17-1979-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.125 µg)/test

View 21 publications 21 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse, Rhesus monkey

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), APC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

3D12

Conjugate

APC APC APC

Excitation/Emission Max

651/660 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1272036

Product Specific Information

Description: The 3D12 monoclonal antibody reacts with human CCR7, also known as EBI-1 and CD197. CCR7 is a member of the G-protein-coupled chemokine receptor family with seven membrane-spanning domains and functions as a receptor for 6Ckine/SLC (secondary lymphoid-tissue chemokine), CCL19 and CCL21. CCR7 has been shown to be internalized via clathrin-coated pits and the majority recycled back to the plasma membrane. CCR7 is expressed on T cells and can be used to distinguish populations of naive from central and effector memory T cells. CCR7 has been shown to play a role in migration of memory T cells to inflamed tissue. Expression of CCR7 is also found on DC's. During DC maturation CCR7 expression increases and is thought to be involved in a variety of functions: chemotaxis to the lymph node, cellular architecture, rate of endocytosis, survival and maturation. Expression of CCR7 on the cell surface can be down regulated upon ligand binding.

Applications Reported: This 3D12 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 3D12 antibody has been pre-titrated and tested by flow cytometric analysis of peripheral blood mononuclear cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

It is recommended that the staining incubation time be increased to at least 45 minutes at 2-8°C for optimal staining.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CCR7 is a member of the G protein coupled receptor family, which is a subfamily of chemokines. CCR7 was identified to be induced by the Epstein Barr virus (EBV), and is thought to be a mediator of EBV effects on B lymphocytes. CCR7 has been reported to be expressed in blood, bone marrow, lymph node, and intestine. CCR7 is particularly expressed in lymphoid tissues and in activated B and T lymphocytes and has been shown to control the migration of memory T cells to inflamed tissues, as well as stimulate dendritic cell maturation. The chemokine (C-C motif) ligand 19 (CCL19/ECL) has been reported to be a specific ligand of this receptor. ESTs have been isolated from blood, embryo, lymph node, and thymus libraries. Receptors for the C - C chemokine family include CCR 1, CCR 2A, CCR 3, CCR 4, CCR 5 and the Duffy blood group antigen. The C-C receptors are important in the function of T cell chemotaxis and migration of phagocytic cells to sites of inflammation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Enhancement of CAR-T cell activity against cholangiocarcinoma by simultaneous knockdown of six inhibitory membrane proteins.

Cancer communications (London, England)

Qiao Y,Chen J,Wang X,Yan S,Tan J,Xia B,Chen Y,Lin K,Zou F,Liu B,He X,Zhang Y,Zhang X,Zhang H,Wu X,Lu L

Published figure using CD197 (CCR7) monoclonal antibody (Product # 17-1979-42) in Flow Cytometry

Sat Jul 01 00:00:00 EDT 2023

Neuroimmune Consequences of eIF4E Phosphorylation on Chemotherapy-Induced Peripheral Neuropathy.

Frontiers in immunology

Agalave NM,Mody PH,Szabo-Pardi TA,Jeong HS,Burton MD

17-1979-42 was used in Flow cytometry/Cell sorting to reveal that cap-dependent translation may be a key pathway that presents relevant therapeutic targets during the early phase of CIPN.

Mon Sep 20 00:00:00 EDT 2021

Neuroimmune Consequences of eIF4E Phosphorylation on Chemotherapy-Induced Peripheral Neuropathy.

Frontiers in immunology

Agalave NM,Mody PH,Szabo-Pardi TA,Jeong HS,Burton MD

17-1979-42 was used in Flow cytometry/Cell sorting to reveal that cap-dependent translation may be a key pathway that presents relevant therapeutic targets during the early phase of CIPN.

Mon Sep 20 00:00:00 EDT 2021

Alteration of the Immune Microenvironment in HBsAg and HBeAg Dual-Positive Pregnant Women Presenting a High HBV Viral Load.

Journal of inflammation research

Gao F,Wang H,Li X,Guo F,Yuan Y,Wang X,Zhang Y,Bai G

Published figure using CD197 (CCR7) monoclonal antibody (Product # 17-1979-42) in Flow Cytometry

Thu Apr 28 00:00:00 EDT 2022

Longitudinal high-throughput TCR repertoire profiling reveals the dynamics of T-cell memory formation after mild COVID-19 infection.

eLife

Minervina AA,Komech EA,Titov A,Bensouda Koraichi M,Rosati E,Mamedov IZ,Franke A,Efimov GA,Chudakov DM,Mora T,Walczak AM,Lebedev YB,Pogorelyy MV

17-1979-42 was used in Flow Cytometry to use longitudinal high-throughput T-cell receptor (TCR) sequencing to track changes in the T-cell repertoire following two mild cases of COVID-19.

Tue Jan 05 00:00:00 EST 2021

Application of the chemokine-chemokine receptor axis increases the tumor-targeted migration ability of cytokine-induced killer cells in patients with colorectal cancer.

Oncology letters

Zou Y,Liang J,Li D,Fang J,Wang L,Wang J,Zhang J,Guo Q,Yan X,Tang H

Published figure using CD197 (CCR7) monoclonal antibody (Product # 17-1979-42) in Flow Cytometry

Wed Jul 01 00:00:00 EDT 2020

Development of CAR-T cell therapy for B-ALL using a point-of-care approach.

Oncoimmunology

de Macedo Abdo L,Barros LRC,Saldanha Viegas M,Vieira Codeço Marques L,de Sousa Ferreira P,Chicaybam L,Bonamino MH

Published figure using CD197 (CCR7) monoclonal antibody (Product # 17-1979-42) in Flow Cytometry

Wed Jul 14 00:00:00 EDT 2021

Single-Cell Profiling Defines Transcriptomic Signatures Specific to Tumor-Reactive versus Virus-Responsive CD4⁺ T Cells.

Cell reports

Magen A,Nie J,Ciucci T,Tamoutounour S,Zhao Y,Mehta M,Tran B,McGavern DB,Hannenhalli S,Bosselut R

Published figure using CD197 (CCR7) monoclonal antibody (Product # 17-1979-42) in Flow Cytometry

Tue Dec 03 00:00:00 EST 2019

Biochanin a Enhances the Defense Against Salmonella enterica Infection Through AMPK/ULK1/mTOR-Mediated Autophagy and Extracellular Traps and Reversing SPI-1-Dependent Macrophage (MΦ) M2 Polarization.

Frontiers in cellular and infection microbiology

Zhao X,Tang X,Guo N,An Y,Chen X,Shi C,Wang C,Li Y,Li S,Xu H,Liu M,Wang Y,Yu L

Published figure using CD197 (CCR7) monoclonal antibody (Product # 17-1979-42) in Flow Cytometry

Fri Aug 30 00:00:00 EDT 2019

Clinically compliant spatial and temporal imaging of chimeric antigen receptor T-cells.

Nature communications

Emami-Shahri N,Foster J,Kashani R,Gazinska P,Cook C,Sosabowski J,Maher J,Papa S

17-1979 was used in Flow cytometry/Cell sorting to show the utility of the human sodium iodide symporter (hNIS) for the temporal and spatial monitoring of CAR T-cell behavior in a cancer-bearing host.

Wed Mar 14 00:00:00 EDT 2018

Role of Nuclear Factor (Erythroid-Derived 2)-Like 2 Signaling for Effects of Fumaric Acid Esters on Dendritic Cells.

Frontiers in immunology

Hammer A,Waschbisch A,Knippertz I,Zinser E,Berg J,Jörg S,Kuhbandner K,David C,Pi J,Bayas A,Lee DH,Haghikia A,Gold R,Steinkasserer A,Linker RA

17-1979 was used in Flow cytometry/Cell sorting to reveal, using treatment with fumaric acid esters as a model, the effects of Nrf2 activation and signalling on dendritic cells.

Wed Nov 20 00:00:00 EST 2019

Oral Supplementation with Baker's Yeast Beta Glucan Is Associated with Altered Monocytes, T Cells and Cytokines following a Bout of Strenuous Exercise.

Frontiers in physiology

McFarlin BK,Venable AS,Carpenter KC,Henning AL,Ogenstad S

Published figure using CD197 (CCR7) monoclonal antibody (Product # 17-1979-42) in Flow Cytometry

Wed Sep 30 00:00:00 EDT 2020

The upregulation of LAG-3 on T cells defines a subpopulation with functional exhaustion and correlates with disease progression in HIV-infected subjects.

Journal of immunology (Baltimore, Md. : 1950)

Tian X,Zhang A,Qiu C,Wang W,Yang Y,Qiu C,Liu A,Zhu L,Yuan S,Hu H,Wang W,Wei Q,Zhang X,Xu J

17-1979 was used in Flow cytometry/Cell sorting to study the role of LAG-3 on T cell functionality during persistent HIV-1 infection.

Wed Apr 15 00:00:00 EDT 2015

Secondary lymphoid organ homing phenotype of human myeloid dendritic cells disrupted by an intracellular oral pathogen.

Infection and immunity

Miles B,Zakhary I,El-Awady A,Scisci E,Carrion J,O'Neill JC,Rawlings A,Stern JK,Susin C,Cutler CW

17-1979 was used in Flow cytometry/Cell sorting to investigate how the alteration in chemokine-chemokine receptor expression contributes to pathogen trafficking via myeloid dendritic cells, showing that P.gingivalis infection induces an angiogenic migratory profile and disrupts secondary lymphoid organ-homing CCR7.

Wed Jan 01 00:00:00 EST 2014

Baseline natural killer and T cell populations correlation with virologic outcome after regimen simplification to atazanavir/ritonavir alone (ACTG 5201).

PloS one

McKinnon JE,Mailliard RB,Swindells S,Wilkin TJ,Borowski L,Roper JM,Bastow B,Kearney M,Wiegand A,Mellors JW,Rinaldo CR

17-1979 was used in Flow cytometry/Cell sorting to test the hypothesis that the level of immune activation (IA) and recovery of lymphocyte populations could influence virologic outcomes after regimen simplification.

Tue Oct 13 00:00:00 EDT 2015

Skewed distribution of IL-7 receptor-α-expressing effector memory CD8+ T cells with distinct functional characteristics in oral squamous cell carcinoma.

PloS one

Lee JJ,Yeh CY,Jung CJ,Chen CW,Du MK,Yu HM,Yang CJ,Lin HY,Sun A,Ko JY,Cheng SJ,Chang YL,Chia JS

17-1979 was used in Flow cytometry/Cell sorting to demonstrate that IL-7 receptor expression on CD8+ T cells may differentiate the CD45RA+/2 CCR72 effector memory CD8+ T-cell subsets that exhibit distinct functional characteristics and association with tumour progression in OSCC patients.

Tue Nov 11 00:00:00 EST 2014

Maintenance of intestinal Th17 cells and reduced microbial translocation in SIV-infected rhesus macaques treated with interleukin (IL)-21.

PLoS pathogens

Pallikkuth S,Micci L,Ende ZS,Iriele RI,Cervasi B,Lawson B,McGary CS,Rogers KA,Else JG,Silvestri G,Easley K,Estes JD,Villinger F,Pahwa S,Paiardini M

17-1979 was used in Flow cytometry/Cell sorting to discover whether IL-21 administration improves mucosal function in the context of pathogenic HIV/SIV infections.

Tue Feb 18 00:00:00 EST 2014

Expression of the memory marker CD45RO on helper T cells in macaques.

PloS one

Valentine M,Song K,Maresh GA,Mack H,Huaman MC,Polacino P,Ho O,Cristillo A,Kyung Chung H,Hu SL,Pincus SH

17-1979 was used in Flow cytometry/Cell sorting to identify and eliminate subsets of T cells known to house a latent reservoir of HIV.

Tue Apr 08 00:00:00 EDT 2014

Expression of the memory marker CD45RO on helper T cells in macaques.

PloS one

Valentine M,Song K,Maresh GA,Mack H,Huaman MC,Polacino P,Ho O,Cristillo A,Kyung Chung H,Hu SL,Pincus SH

17-1979 was used in Flow cytometry/Cell sorting to identify and eliminate subsets of T cells known to house a latent reservoir of HIV.

Tue Apr 08 00:00:00 EDT 2014

Haemophilus ducreyi-induced interleukin-10 promotes a mixed M1 and M2 activation program in human macrophages.

Infection and immunity

Li W,Katz BP,Spinola SM

17-1979 was used in Flow cytometry/Cell sorting to investigate the contribution of IL-10-modulated macrophage polarisation to H. ducreyi clearance during human infection.

Sat Dec 01 00:00:00 EST 2012

Vitamin D suppression of endoplasmic reticulum stress promotes an antiatherogenic monocyte/macrophage phenotype in type 2 diabetic patients.

The Journal of biological chemistry

Riek AE,Oh J,Sprague JE,Timpson A,de las Fuentes L,Bernal-Mizrachi L,Schechtman KB,Bernal-Mizrachi C

17-1979 was used in Flow cytometry/Cell sorting to investigate the the possible role of vitamin D deficiency as a mechanism of increased atherosclerosis in T2DM patients.

Fri Nov 09 00:00:00 EST 2012

Human CD8⁺ and CD4⁺ T cell memory to lymphocytic choriomeningitis virus infection.

Journal of virology

Kotturi MF,Swann JA,Peters B,Arlehamn CL,Sidney J,Kolla RV,James EA,Akondy RS,Ahmed R,Kwok WW,Buchmeier MJ,Sette A

17-1979 was used in Flow cytometry/Cell sorting to analyse the T cell response to acute lymphocytic choriomeningitis virus infection.

Tue Nov 01 00:00:00 EDT 2011

Role of TLX1 in T-cell acute lymphoblastic leukaemia pathogenesis.

British journal of haematology

Riz I,Hawley TS,Johnston H,Hawley RG

Published figure using CD197 (CCR7) monoclonal antibody (Product # 17-1979-42) in Flow Cytometry

Wed Apr 01 00:00:00 EDT 2009

Bioinformatics

Protein Aliases: BLR2; Bukitt's lymphoma receptor 2; C-C chemokine receptor type 7; C-C CKR-7; CC chemokine receptor 7; CD197; CDw197; chemokine (C-C motif) receptor 7; EBI1; EBV-induced G protein-coupled receptor 1; Epstein-Barr virus induced gene 1; Epstein-Barr virus-induced G-protein coupled receptor 1; lymphocyte-specific G protein-coupled peptide receptor; MIP-3 beta receptor

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Gene Aliases: BLR2; CC-CKR-7; CCR-7; CCR7; CD197; CDw197; CMKBR7; EBI1; EVI1

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UniProt ID: (Human) P32248

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Entrez Gene ID: (Human) 1236

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Function(s)

G-protein coupled receptor activity C-C chemokine receptor activity chemokine (C-C motif) ligand 19 binding chemokine (C-C motif) ligand 21 binding C-C motif chemokine 19 receptor activity C-C motif chemokine 21 receptor activity

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD197 (CCR7) Monoclonal Antibody (3D12), APC, eBioscience™

CD197 (CCR7) Antibody (17-1979-42) in Flow

Product Details

17-1979-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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