Invitrogen

CD197 (CCR7) Monoclonal Antibody (3D12), PE-Cyanine7, eBioscience™

9 Published Figures

17 References

View all (60) CCR7 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (9)

CD197 (CCR7) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 10

CD197 (CCR7) Antibody (25-1979-42) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD45RA APC (Product # 17-0458-42) and Rat IgG2a K Isotype Control PE-Cyanine7 (Product # 25-4321-82) (left) or Anti-Human CD197 (CCR7) PE-Cyanine7 (right). Cells in the lymphocyte gate were used for analysis.

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Product Details

25-1979-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.25 µg)/test

View 17 publications 17 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), PE-Cyanine7, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

3D12

Conjugate

PE-Cyanine7 PE-Cyanine7 PE-Cyanine7

Excitation/Emission Max

569/780 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2573422

Product Specific Information

Description: The 3D12 monoclonal antibody reacts with human CCR7, also known as EBI-1 and CD197. CCR7 is a member of the G-protein-coupled chemokine receptor family with seven membrane-spanning domains and functions as a receptor for 6Ckine/SLC (secondary lymphoid-tissue chemokine), CCL19 and CCL21. CCR7 has been shown to be internalized via clathrin-coated pits and the majority recycled back to the plasma membrane. CCR7 is expressed on T cells and can be used to distinguish populations of naive from central and effector memory T cells. CCR7 has been shown to play a role in migration of memory T cells to inflamed tissue. Expression of CCR7 is also found on DC's. During DC maturation CCR7 expression increases and is thought to be involved in a variety of functions: chemotaxis to the lymph node, cellular architecture, rate of endocytosis, survival and maturation. Expression of CCR7 on the cell surface can be down regulated upon ligand binding.

Applications Reported: This 3D12 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 3D12 antibody has been pre-titrated and tested by flow cytometric analysis of normal human perioheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

It is recommended that the staining incubation time be increased to at least 45 minutes at 2-8°C.

Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CCR7 is a member of the G protein coupled receptor family, which is a subfamily of chemokines. CCR7 was identified to be induced by the Epstein Barr virus (EBV), and is thought to be a mediator of EBV effects on B lymphocytes. CCR7 has been reported to be expressed in blood, bone marrow, lymph node, and intestine. CCR7 is particularly expressed in lymphoid tissues and in activated B and T lymphocytes and has been shown to control the migration of memory T cells to inflamed tissues, as well as stimulate dendritic cell maturation. The chemokine (C-C motif) ligand 19 (CCL19/ECL) has been reported to be a specific ligand of this receptor. ESTs have been isolated from blood, embryo, lymph node, and thymus libraries. Receptors for the C - C chemokine family include CCR 1, CCR 2A, CCR 3, CCR 4, CCR 5 and the Duffy blood group antigen. The C-C receptors are important in the function of T cell chemotaxis and migration of phagocytic cells to sites of inflammation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Enhancement of CAR-T cell activity against cholangiocarcinoma by simultaneous knockdown of six inhibitory membrane proteins.

Cancer communications (London, England)

Qiao Y,Chen J,Wang X,Yan S,Tan J,Xia B,Chen Y,Lin K,Zou F,Liu B,He X,Zhang Y,Zhang X,Zhang H,Wu X,Lu L

Published figure using CD197 (CCR7) monoclonal antibody (Product # 25-1979-42) in Flow Cytometry

Sat Jul 01 00:00:00 EDT 2023

Neuroimmune Consequences of eIF4E Phosphorylation on Chemotherapy-Induced Peripheral Neuropathy.

Frontiers in immunology

Agalave NM,Mody PH,Szabo-Pardi TA,Jeong HS,Burton MD

Published figure using CD197 (CCR7) monoclonal antibody (Product # 25-1979-42) in Flow Cytometry

Mon Sep 20 00:00:00 EDT 2021

Alteration of the Immune Microenvironment in HBsAg and HBeAg Dual-Positive Pregnant Women Presenting a High HBV Viral Load.

Journal of inflammation research

Gao F,Wang H,Li X,Guo F,Yuan Y,Wang X,Zhang Y,Bai G

Published figure using CD197 (CCR7) monoclonal antibody (Product # 25-1979-42) in Flow Cytometry

Thu Apr 28 00:00:00 EDT 2022

Monocyte-driven atypical cytokine storm and aberrant neutrophil activation as key mediators of COVID-19 disease severity.

Nature communications

Vanderbeke L,Van Mol P,Van Herck Y,De Smet F,Humblet-Baron S,Martinod K,Antoranz A,Arijs I,Boeckx B,Bosisio FM,Casaer M,Dauwe D,De Wever W,Dooms C,Dreesen E,Emmaneel A,Filtjens J,Gouwy M,Gunst J,Hermans G,Jansen S,Lagrou K,Liston A,Lorent N,Meersseman P,Mercier T,Neyts J,Odent J,Panovska D,Penttila PA,Pollet E,Proost P,Qian J,Quintelier K,Raes J,Rex S,Saeys Y,Sprooten J,Tejpar S,Testelmans D,Thevissen K,Van Buyten T,Vandenhaute J,Van Gassen S,Velásquez Pereira LC,Vos R,Weynand B,Wilmer A,Yserbyt J,Garg AD,Matthys P,Wouters C,Lambrechts D,Wauters E,Wauters J

25-1979-42 was used in Flow Cytometry to suggest a myeloid-driven immunopathology, in which hyperactivated neutrophils and an ineffective adaptive immune system act as mediators of COVID-19 disease severity.

Mon Jul 05 00:00:00 EDT 2021

Microglia Require CD4 T Cells to Complete the Fetal-to-Adult Transition.

Cell

Pasciuto E,Burton OT,Roca CP,Lagou V,Rajan WD,Theys T,Mancuso R,Tito RY,Kouser L,Callaerts-Vegh Z,de la Fuente AG,Prezzemolo T,Mascali LG,Brajic A,Whyte CE,Yshii L,Martinez-Muriana A,Naughton M,Young A,Moudra A,Lemaitre P,Poovathingal S,Raes J,De Strooper B,Fitzgerald DC,Dooley J,Liston A

25-1979 was used in Flow cytometry/Cell sorting to investigate the function of brain-resident CD4 T cells.

Thu Aug 06 00:00:00 EDT 2020

Application of the chemokine-chemokine receptor axis increases the tumor-targeted migration ability of cytokine-induced killer cells in patients with colorectal cancer.

Oncology letters

Zou Y,Liang J,Li D,Fang J,Wang L,Wang J,Zhang J,Guo Q,Yan X,Tang H

Published figure using CD197 (CCR7) monoclonal antibody (Product # 25-1979-42) in Flow Cytometry

Wed Jul 01 00:00:00 EDT 2020

Development of CAR-T cell therapy for B-ALL using a point-of-care approach.

Oncoimmunology

de Macedo Abdo L,Barros LRC,Saldanha Viegas M,Vieira Codeço Marques L,de Sousa Ferreira P,Chicaybam L,Bonamino MH

Published figure using CD197 (CCR7) monoclonal antibody (Product # 25-1979-42) in Flow Cytometry

Wed Jul 14 00:00:00 EDT 2021

Frequency determination of breast tumor-reactive CD4 and CD8 T cells in humans: unveiling the antitumor immune response.

Oncoimmunology

Pinho MP,Patente TA,Flatow EA,Sallusto F,Barbuto JAM

25-1979-42 was used in Flow cytometry/Cell sorting to describe a strategy that allows this determination, in both CD4 and CD8 compartments, using T cell proliferation induced by tumor cell-lysate pulsed dendritic cells as the readout.

Tue Jan 12 00:00:00 EST 2021

Biochanin a Enhances the Defense Against Salmonella enterica Infection Through AMPK/ULK1/mTOR-Mediated Autophagy and Extracellular Traps and Reversing SPI-1-Dependent Macrophage (MΦ) M2 Polarization.

Frontiers in cellular and infection microbiology

Zhao X,Tang X,Guo N,An Y,Chen X,Shi C,Wang C,Li Y,Li S,Xu H,Liu M,Wang Y,Yu L

Published figure using CD197 (CCR7) monoclonal antibody (Product # 25-1979-42) in Flow Cytometry

Fri Aug 30 00:00:00 EDT 2019

The influence of chemotherapy on adenosine-producing B cells in patients with head and neck squamous cell carcinoma.

Oncotarget

Ziebart A,Huber U,Jeske S,Laban S,Doescher J,Hoffmann TK,Brunner C,Jackson EK,Schuler PJ

25-1979 was used in Flow cytometry/Cell sorting to study the influence of chemotherapy on the function of regulatory B cells.

Fri Jan 19 00:00:00 EST 2018

Targeting VEGFR2 with Ramucirumab strongly impacts effector/ activated regulatory T cells and CD8⁺ T cells in the tumor microenvironment.

Journal for immunotherapy of cancer

Tada Y,Togashi Y,Kotani D,Kuwata T,Sato E,Kawazoe A,Doi T,Wada H,Nishikawa H,Shitara K

25-1979 was used in Flow cytometry/Cell sorting to study the effects of ramucirumab on the tumour microenvironmental immune status in human cancers.

Thu Oct 11 00:00:00 EDT 2018

Renal Sodium Gradient Orchestrates a Dynamic Antibacterial Defense Zone.

Cell

Berry MR,Mathews RJ,Ferdinand JR,Jing C,Loudon KW,Wlodek E,Dennison TW,Kuper C,Neuhofer W,Clatworthy MR

25-1979-42 was used in Flow Cytometry to reveal a critical accessory role for the homeostatic function of a vital organ in optimizing tissue defense.

Thu Aug 24 00:00:00 EDT 2017

Oral Supplementation with Baker's Yeast Beta Glucan Is Associated with Altered Monocytes, T Cells and Cytokines following a Bout of Strenuous Exercise.

Frontiers in physiology

McFarlin BK,Venable AS,Carpenter KC,Henning AL,Ogenstad S

Published figure using CD197 (CCR7) monoclonal antibody (Product # 25-1979-42) in Flow Cytometry

Wed Sep 30 00:00:00 EDT 2020

Activation of Innate and Adaptive Immunity by a Recombinant Human Cytomegalovirus Strain Expressing an NKG2D Ligand.

PLoS pathogens

Tomić A,Varanasi PR,Golemac M,Malić S,Riese P,Borst EM,Mischak-Weissinger E,Guzmán CA,Krmpotić A,Jonjić S,Messerle M

25-1979 was used in Flow cytometry/Cell sorting to investigate a strategy for an HCMV vaccine that aims at the simultaneous activation of innate and adaptive immune responses.

Thu Dec 01 00:00:00 EST 2016

Interleukin‑33‑induced immune tolerance is associated with the imbalance of memory and naïve T‑lymphocyte subsets.

Molecular medicine reports

Shi X,Tang Y,Sun X,Liu Y,Sun Y,Sun M,Jiang Y,Li Y

25-1979 was used in Flow cytometry/Cell sorting to investigate the distribution of memory and naive T cells in hepatitis B virus-infected patients at different immune stages, and the effect of interleukin-33 on the regulation of the T-cell subsets.

Tue Nov 01 00:00:00 EDT 2016

Impact of Pre-Transplant Anti-T Cell Globulin (ATG) on Immune Recovery after Myeloablative Allogeneic Peripheral Blood Stem Cell Transplantation.

PloS one

Servais S,Menten-Dedoyart C,Beguin Y,Seidel L,Gothot A,Daulne C,Willems E,Delens L,Humblet-Baron S,Hannon M,Baron F

25-1979 was used in Flow cytometry/Cell sorting to assess the impact of anti-T cell globulin on the recovery of a large variety of lymphoid cell subsets as well as on thymic function after myeloablative peripheral blood stem cell transplantation.

Fri Apr 22 00:00:00 EDT 2016

Role of TLX1 in T-cell acute lymphoblastic leukaemia pathogenesis.

British journal of haematology

Riz I,Hawley TS,Johnston H,Hawley RG

Published figure using CD197 (CCR7) monoclonal antibody (Product # 25-1979-42) in Flow Cytometry

Wed Apr 01 00:00:00 EDT 2009

Bioinformatics

Protein Aliases: BLR2; Bukitt's lymphoma receptor 2; C-C chemokine receptor type 7; C-C CKR-7; CC chemokine receptor 7; CD197; CDw197; chemokine (C-C motif) receptor 7; EBI1; EBV-induced G protein-coupled receptor 1; Epstein-Barr virus induced gene 1; Epstein-Barr virus-induced G-protein coupled receptor 1; lymphocyte-specific G protein-coupled peptide receptor; MIP-3 beta receptor

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Gene Aliases: BLR2; CC-CKR-7; CCR-7; CCR7; CD197; CDw197; CMKBR7; EBI1; EVI1

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UniProt ID: (Human) P32248

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Entrez Gene ID: (Human) 1236

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Function(s)

G-protein coupled receptor activity C-C chemokine receptor activity chemokine (C-C motif) ligand 19 binding chemokine (C-C motif) ligand 21 binding C-C motif chemokine 19 receptor activity C-C motif chemokine 21 receptor activity

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD197 (CCR7) Monoclonal Antibody (3D12), PE-Cyanine7, eBioscience™

CD197 (CCR7) Antibody (25-1979-42) in Flow

Product Details

25-1979-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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