Invitrogen

CD140b (PDGFRB) Monoclonal Antibody (APB5), APC, eBioscience™

CD140b (PDGFRB) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 24

CD140b (PDGFRB) Antibody (17-1402-82) in Flow

Staining of the NIH/3T3 cell line with 0.25 µg of Rat IgG2a kappa Isotype Control APC (Product # 17-4321-81) (blue histogram) or 0.25 µg of Anti-Mouse CD140b (PDGF Receptor b) APC (purple histogram). Total viable cells were used for analysis.

CD140b (PDGFRB) Antibody in Immunohistochemistry (IHC)

Product Image Gallery

Product Details

17-1402-82

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Flow Cytometry (Flow)

0.5 µg/test

View 28 publications 28 publications

Product Specifications

Species Reactivity

Mouse

Published species

Fish, Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), APC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

APB5

Conjugate

APC APC APC

Excitation/Emission Max

651/660 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1548743

Product Specific Information

Description: The APB5 monoclonal antibody reacts with the mouse CD140b molecule, the beta chain of the platelet derived growth factor receptor (PDGF receptor). PDGFRb is a receptor tyrosine kinase that forms dimers on the surface upon ligand binding and phosphorylates substrates. Dimers of PDGFR consist of either homodimers of alpha/alpha, beta/beta, or heterodimers of alpha/beta and serve as a substrate for its kinase activity. CD140b is expressed by embryonic tissues and mesenchymal-derived cells of the adult mouse tissues. The PDGFR beta chain is reported to play a significant role in formation of fibrous atherosclerotic lesions.

Applications Reported: This APB5 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This APB5 antibody has been tested by flow cytometric analysis of NIH 3T3 cell line. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

PDGFRB is a receptor tyrosine kinase and has been implicated in atherogenesis and in cell transformation by the SIS oncogene. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. The gene is flanked on chromosome 5 by the genes for granulocyte-macrophage colony-stimulating factor and macrophage-colony stimulating factor receptor; all three genes may be implicated in the 5-q syndrome. A translocation between chromosomes 5 and 12, that fuses PDGFRB (CD140b) to that of the translocation, ETV6, leukemia gene, results in chronic myeloproliferative disorder with eosinophilia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Type H vessel/platelet-derived growth factor receptor β⁺ perivascular cell disintegration is involved in vascular injury and bone loss in radiation-induced bone damage.

Cell proliferation

Li J,Chen X,Ren L,Chen X,Wu T,Wang Y,Ren X,Cheng B,Xia J

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 17-1402-82) in Flow Cytometry

Sat Jul 01 00:00:00 EDT 2023

PDGFRβ⁺ cells play a dual role as hematopoietic precursors and niche cells during mouse ontogeny.

Cell reports

Sá da Bandeira D,Kilpatrick AM,Marques M,Gomez-Salazar M,Ventura T,Gonzalez ZN,Stefancova D,Rossi F,Vermeren M,Vink CS,Beltran M,Henderson NC,Jung B,van der Linden R,van de Werken HJG,van Ijcken WFJ,Betsholtz C,Forbes SJ,Cuervo H,Crisan M

17-1402-82 was used in Flow cytometry/Cell sorting to demonstrate that PDGFRβ+ cells play a dual role in murine hematopoiesis.

Tue Jul 19 00:00:00 EDT 2022

Heterogeneous bone-marrow stromal progenitors drive myelofibrosis via a druggable alarmin axis.

Cell stem cell

Leimkühler NB,Gleitz HFE,Ronghui L,Snoeren IAM,Fuchs SNR,Nagai JS,Banjanin B,Lam KH,Vogl T,Kuppe C,Stalmann USA,Büsche G,Kreipe H,Gütgemann I,Krebs P,Banz Y,Boor P,Tai EW,Brümmendorf TH,Koschmieder S,Crysandt M,Bindels E,Kramann R,Costa IG,Schneider RK

17-1402-82 was used in Flow Cytometry to develop a comprehensive map of the stroma in myeloproliferative neoplasms (MPNs)on a single-cell level in murine models and patient samples.

Thu Apr 01 00:00:00 EDT 2021

Cellular dormancy in minimal residual disease following targeted therapy.

Breast cancer research : BCR

Ruth JR,Pant DK,Pan TC,Seidel HE,Baksh SC,Keister BA,Singh R,Sterner CJ,Bakewell SJ,Moody SE,Belka GK,Chodosh LA

17-1402-82 was used in Flow Cytometry to identify residual tumour cell-specific features using transgenic mouse models.

Fri Jun 04 00:00:00 EDT 2021

Cellular dormancy in minimal residual disease following targeted therapy.

Breast cancer research : BCR

Ruth JR,Pant DK,Pan TC,Seidel HE,Baksh SC,Keister BA,Singh R,Sterner CJ,Bakewell SJ,Moody SE,Belka GK,Chodosh LA

17-1402-82 was used in Flow Cytometry to identify residual tumour cell-specific features using transgenic mouse models.

Fri Jun 04 00:00:00 EDT 2021

Distinct Fibroblast Lineages Give Rise to NG2+ Pericyte Populations in Mouse Skin Development and Repair.

Frontiers in cell and developmental biology

Goss G,Rognoni E,Salameti V,Watt FM

17-1402-82 was used in Flow Cytometry to investigate if Ng2+ perivascular cells represent a heterogeneous lineage restricted population and if it is primarily recruited from the papillary or reticular fibroblast lineages during tissue regeneration.

Wed Mar 02 00:00:00 EST 2022

Distinct Fibroblast Lineages Give Rise to NG2+ Pericyte Populations in Mouse Skin Development and Repair.

Frontiers in cell and developmental biology

Goss G,Rognoni E,Salameti V,Watt FM

Wed Mar 02 00:00:00 EST 2022

Direct contribution of skeletal muscle mesenchymal progenitors to bone repair.

Nature communications

Julien A,Kanagalingam A,Martínez-Sarrà E,Megret J,Luka M,Ménager M,Relaix F,Colnot C

17-1402 was used in Flow cytometry/Cell sorting to elucidate the central role of skeletal muscle in bone regeneration and provide evidence that skeletal muscle can be targeted to prevent persistent callus fibrosis and improve bone healing after musculoskeletal trauma.

Mon May 17 00:00:00 EDT 2021

Direct contribution of skeletal muscle mesenchymal progenitors to bone repair.

Nature communications

Julien A,Kanagalingam A,Martínez-Sarrà E,Megret J,Luka M,Ménager M,Relaix F,Colnot C

Mon May 17 00:00:00 EDT 2021

Cardiac pericytes function as key vasoactive cells to regulate homeostasis and disease.

FEBS open bio

Lee LL,Khakoo AY,Chintalgattu V

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 17-1402-82) in Flow Cytometry

Fri Jan 01 00:00:00 EST 2021

Heterogeneity of murine periosteum progenitors involved in fracture healing.

eLife

Matthews BG,Novak S,Sbrana FV,Funnell JL,Cao Y,Buckels EJ,Grcevic D,Kalajzic I

17-1402-82 was used in Flow Cytometry to demonstrate that the periosteum is highly enriched with skeletal progenitor cells, and there is heterogeneity in the populations of cells that contribute to mature lineages during periosteal fracture healing.

Tue Feb 09 00:00:00 EST 2021

Metastasis-initiating cells induce and exploit a fibroblast niche to fuel malignant colonization of the lungs.

Nature communications

Pein M,Insua-Rodríguez J,Hongu T,Riedel A,Meier J,Wiedmann L,Decker K,Essers MAG,Sinn HP,Spaich S,Sütterlin M,Schneeweiss A,Trumpp A,Oskarsson T

17-1402-82 was used in Flow Cytometry to show that disseminated breast cancer cells evoke phenotypic changes in lung fibroblasts, forming a supportive metastatic niche.

Fri Mar 20 00:00:00 EDT 2020

Dual lineage tracing identifies intermediate mesenchymal stage for endocardial contribution to fibroblasts, coronary mural cells, and adipocytes.

The Journal of biological chemistry

Huang X,Feng T,Jiang Z,Meng J,Kou S,Lu Z,Chen W,Lin CP,Zhou B,Zhang H

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 17-1402-82) in Flow Cytometry

Fri May 31 00:00:00 EDT 2019

Growth Factor Screening in Dystrophic Muscles Reveals PDGFB/PDGFRB-Mediated Migration of Interstitial Stem Cells.

International journal of molecular sciences

Camps J,Grosemans H,Gijsbers R,Maes C,Sampaolesi M

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 17-1402-82) in Flow Cytometry

Tue Mar 05 00:00:00 EST 2019

Bone marrow drives central nervous system regeneration after radiation injury.

The Journal of clinical investigation

Dietrich J,Baryawno N,Nayyar N,Valtis YK,Yang B,Ly I,Besnard A,Severe N,Gustafsson KU,Andronesi OC,Batchelor TT,Sahay A,Scadden DT

17-1402 was used in Flow cytometry/Cell sorting to demonstrate that the hematopoietic system provides critical regenerative drive to the brain that can be modulated by clinically available agents.

Tue Jan 02 00:00:00 EST 2018

MicroRNA-Mediated Therapy Modulating Blood-Brain Barrier Disruption Improves Vascular Cognitive Impairment.

Arteriosclerosis, thrombosis, and vascular biology

Toyama K,Spin JM,Deng AC,Huang TT,Wei K,Wagenhäuser MU,Yoshino T,Nguyen H,Mulorz J,Kundu S,Raaz U,Adam M,Schellinger IN,Jagger A,Tsao PS

17-1402-82 was used in Flow Cytometry to provide the first evidence that the TNFα-miR-501-3p-ZO-1 axis plays an important role in the pathogenesis of cerebral hypoperfusion-induced working memory deficits and white matter lesions, as a result of blood-brain barrier breakdown via tight junction disruption.

Fri Jun 01 00:00:00 EDT 2018

Pulmonary pericytes regulate lung morphogenesis.

Nature communications

Kato K,Diéguez-Hurtado R,Park DY,Hong SP,Kato-Azuma S,Adams S,Stehling M,Trappmann B,Wrana JL,Koh GY,Adams RH

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 17-1402-82) in Flow Cytometry

Fri Jun 22 00:00:00 EDT 2018

Pulmonary pericytes regulate lung morphogenesis.

Nature communications

Kato K,Diéguez-Hurtado R,Park DY,Hong SP,Kato-Azuma S,Adams S,Stehling M,Trappmann B,Wrana JL,Koh GY,Adams RH

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 17-1402-82) in Flow Cytometry

Fri Jun 22 00:00:00 EDT 2018

Gpr124 is essential for blood-brain barrier integrity in central nervous system disease.

Nature medicine

Chang J,Mancuso MR,Maier C,Liang X,Yuki K,Yang L,Kwong JW,Wang J,Rao V,Vallon M,Kosinski C,Zhang JJ,Mah AT,Xu L,Li L,Gholamin S,Reyes TF,Li R,Kuhnert F,Han X,Yuan J,Chiou SH,Brettman AD,Daly L,Corney DC,Cheshier SH,Shortliffe LD,Wu X,Snyder M,Chan P,Giffard RG,Chang HY,Andreasson K,Kuo CJ

17-1402 was used in Flow cytometry/Cell sorting to examine the function of the endothelial G-protein-coupled receptor Gpr124 in homeostasis and pathology in adult mice.

Sat Apr 01 00:00:00 EDT 2017

Differential cytokine contributions of perivascular haematopoietic stem cell niches.

Nature cell biology

Asada N,Kunisaki Y,Pierce H,Wang Z,Fernandez NF,Birbrair A,Ma'ayan A,Frenette PS

17-1402 was used in Flow cytometry/Cell sorting to demonstrate that the effects of cytokines regulating haematopoietic stem cell functions are dependent on the producing cell sources.

Wed Mar 01 00:00:00 EST 2017

Cardiac Fibroblasts Adopt Osteogenic Fates and Can Be Targeted to Attenuate Pathological Heart Calcification.

Cell stem cell

Pillai ICL,Li S,Romay M,Lam L,Lu Y,Huang J,Dillard N,Zemanova M,Rubbi L,Wang Y,Lee J,Xia M,Liang O,Xie YH,Pellegrini M,Lusis AJ,Deb A

17-1402 was used in Flow cytometry/Cell sorting to study the cell identify and mechanisms contributing to pathological heart muscle calcification.

Thu Feb 02 00:00:00 EST 2017

Laminin differentially regulates the stemness of type I and type II pericytes.

Stem cell research & therapy

Gautam J,Nirwane A,Yao Y

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 17-1402-82) in Flow Cytometry

Tue Feb 07 00:00:00 EST 2017

Neonatal pancreatic pericytes support β-cell proliferation.

Molecular metabolism

Epshtein A,Rachi E,Sakhneny L,Mizrachi S,Baer D,Landsman L

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 17-1402-82) in Flow Cytometry

Sun Oct 01 00:00:00 EDT 2017

Pericytes regulate VEGF-induced endothelial sprouting through VEGFR1.

Nature communications

Eilken HM,Diéguez-Hurtado R,Schmidt I,Nakayama M,Jeong HW,Arf H,Adams S,Ferrara N,Adams RH

17-1402 was used in Flow cytometry/Cell sorting to show that pericytes promote endothelial sprouting in the postnatal retinal vasculature.

Fri Nov 17 00:00:00 EST 2017

Age-dependent modulation of vascular niches for haematopoietic stem cells.

Nature

Kusumbe AP,Ramasamy SK,Itkin T,Mäe MA,Langen UH,Betsholtz C,Lapidot T,Adams RH

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 17-1402-82) in Flow Cytometry

Thu Apr 21 00:00:00 EDT 2016

Paracrine Wnt1 Drives Interstitial Fibrosis without Inflammation by Tubulointerstitial Cross-Talk.

Journal of the American Society of Nephrology : JASN

Maarouf OH,Aravamudhan A,Rangarajan D,Kusaba T,Zhang V,Welborn J,Gauvin D,Hou X,Kramann R,Humphreys BD

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 17-1402-82) in Flow Cytometry

Tue Mar 01 00:00:00 EST 2016

Analysis of the brain mural cell transcriptome.

Scientific reports

He L,Vanlandewijck M,Raschperger E,Andaloussi Mäe M,Jung B,Lebouvier T,Ando K,Hofmann J,Keller A,Betsholtz C

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 17-1402-82) in Flow Cytometry

Tue Oct 11 00:00:00 EDT 2016

Endothelial cells are progenitors of cardiac pericytes and vascular smooth muscle cells.

Nature communications

Chen Q,Zhang H,Liu Y,Adams S,Eilken H,Stehling M,Corada M,Dejana E,Zhou B,Adams RH

17-1402 was used in Flow cytometry/Cell sorting to determine that Endothelial cells are progenitors of cardiac pericytes and vascular smooth muscle cells.

Fri Aug 12 00:00:00 EDT 2016

Mesodermal iPSC-derived progenitor cells functionally regenerate cardiac and skeletal muscle.

The Journal of clinical investigation

Quattrocelli M,Swinnen M,Giacomazzi G,Camps J,Barthélemy I,Ceccarelli G,Caluwé E,Grosemans H,Thorrez L,Pelizzo G,Muijtjens M,Verfaillie CM,Blot S,Janssens S,Sampaolesi M

17-1402 was used in Flow cytometry/Cell sorting to study the functional regeneration of cardiac and skeletal muscle via mesodermal iPSC-derived progenitor cells.

Tue Dec 01 00:00:00 EST 2015

Regional and stage-specific effects of prospectively purified vascular cells on the adult V-SVZ neural stem cell lineage.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Crouch EE,Liu C,Silva-Vargas V,Doetsch F

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 17-1402-82) in Flow Cytometry

Wed Mar 18 00:00:00 EDT 2015

Pericytes derived from adipose-derived stem cells protect against retinal vasculopathy.

PloS one

Mendel TA,Clabough EB,Kao DS,Demidova-Rice TN,Durham JT,Zotter BC,Seaman SA,Cronk SM,Rakoczy EP,Katz AJ,Herman IM,Peirce SM,Yates PA

17-1402 was used in Flow cytometry/Cell sorting to examine the ability of ASCs to differentiate into pericytes that can stabilize retinal vessels in retinal vasculopathy models.

Fri Jan 10 00:00:00 EST 2014

Differential contribution to neuroendocrine tumorigenesis of parallel egfr signaling in cancer cells and pericytes.

Genes & cancer

Nolan-Stevaux O,Truitt MC,Pahler JC,Olson P,Guinto C,Lee DC,Hanahan D

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 17-1402-82) in Flow Cytometry

Mon Feb 01 00:00:00 EST 2010

Bioinformatics

Protein Aliases: Beta platelet-derived growth factor receptor; Beta-type platelet-derived growth factor receptor; CD140 antigen-like family member B; CD140b; CD140b antigen; PDGF beta chain; PDGF Receptor beta; PDGF-R-beta; PDGFR-1; PDGFR-beta; Platelet-derived growth factor receptor 1; Platelet-derived growth factor receptor beta; platelet-derived growth factor receptor beta variant 1

View more View less

Gene Aliases: AI528809; CD140b; Pdgfr; PDGFR-1; Pdgfr1; Pdgfrb

View more View less

UniProt ID: (Mouse) P05622

View more View less

Entrez Gene ID: (Mouse) 18596

View more View less

Function(s)

nucleotide binding protein kinase activity protein tyrosine kinase activity transmembrane receptor protein tyrosine kinase activity signal transducer activity platelet-derived growth factor-activated receptor activity platelet-derived growth factor beta-receptor activity receptor binding platelet-derived growth factor receptor binding protein binding ATP binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups protein kinase binding vascular endothelial growth factor binding phosphatidylinositol 3-kinase binding platelet-derived growth factor binding transmembrane signal receptor

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

CD140b (PDGFRB) Monoclonal Antibody (APB5), APC, eBioscience™

CD140b (PDGFRB) Antibody (17-1402-82) in Flow

Product Details

17-1402-82

Immunohistochemistry (IHC)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!