Invitrogen

CD140b (PDGFRB) Monoclonal Antibody (APB5), Super Bright™ 436, eBioscience™

CD140b (PDGFRB) Antibody in Flow Cytometry (Flow)

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CD140b (PDGFRB) Antibody (62-1402-82) in Flow

Staining of the NIH/3T3 cell line with 0.5 µg of Rat IgG2a kappa Isotype Control, Super Bright 436 (Product # 62-4321-82) (blue histogram) or 0.5 µg of CD140b (PDGF Receptor b) Monoclonal Antibody, Super Bright 436 (purple histogram). Total viable cells, as determined by Fixable Viability Dye eFluor 780 (Product # 65-0865-18), were used for analysis.

CD140b (PDGFRB) Antibody in Immunohistochemistry (IHC)

CD140b (PDGFRB) Antibody in Immunocytochemistry (ICC/IF)

CD140b (PDGFRB) Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)

CD140b (PDGFRB) Antibody in Immunocytochemistry, Immunohistochemistry (Frozen) (ICC/IF, IHC (F))

CD140b (PDGFRB) Antibody in Immunohistochemistry, Flow Cytometry (IHC, Flow)

CD140b (PDGFRB) Antibody in Neutralization (Neu)

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Product Details

62-1402-82

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 6 publications 6 publications

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 7 publications 7 publications

Flow Cytometry (Flow)

1.0 µg/test

View 7 publications 7 publications

Neutralization (Neu)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Not Applicable

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), Super Bright™ 436, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

APB5

Conjugate

Super Bright™ 436 Super Bright™ 436 Super Bright™ 436

Excitation/Emission Max

413/431 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2744790

Product Specific Information

Description: The APB5 monoclonal antibody reacts with the mouse CD140b molecule, the beta chain of the platelet derived growth factor receptor (PDGF receptor). PDGFRb is a receptor tyrosine kinase that forms dimers on the surface upon ligand binding and phosphorylates substrates. Dimers of PDGFR consist of either homodimers of alpha/alpha, beta/beta, or heterodimers of alpha/beta and serve as a substrate for its kinase activity. CD140b is expressed by embryonic tissues and mesenchymal-derived cells of the adult mouse tissues. The PDGFR beta chain is reported to play a significant role in formation of fibrous atherosclerotic lesions.

Applications Reported: The APB5 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The APB5 antibody has been tested by flow cytometric analysis of NIH/3T3 cells. This can be used at less than or equal to 1.0 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm. We recommend using a 450/50 bandpass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome.

When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.

Excitation: 405 nm; Emission: 436 nm; Laser: Violet Laser

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

PDGFRB is a receptor tyrosine kinase and has been implicated in atherogenesis and in cell transformation by the SIS oncogene. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. The gene is flanked on chromosome 5 by the genes for granulocyte-macrophage colony-stimulating factor and macrophage-colony stimulating factor receptor; all three genes may be implicated in the 5-q syndrome. A translocation between chromosomes 5 and 12, that fuses PDGFRB (CD140b) to that of the translocation, ETV6, leukemia gene, results in chronic myeloproliferative disorder with eosinophilia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Type H vessel/platelet-derived growth factor receptor β⁺ perivascular cell disintegration is involved in vascular injury and bone loss in radiation-induced bone damage.

Cell proliferation

Li J,Chen X,Ren L,Chen X,Wu T,Wang Y,Ren X,Cheng B,Xia J

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 62-1402-82) in Flow Cytometry

Sat Jul 01 00:00:00 EDT 2023

Cellular dormancy in minimal residual disease following targeted therapy.

Breast cancer research : BCR

Ruth JR,Pant DK,Pan TC,Seidel HE,Baksh SC,Keister BA,Singh R,Sterner CJ,Bakewell SJ,Moody SE,Belka GK,Chodosh LA

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 62-1402-82) in Flow Cytometry

Fri Jun 04 00:00:00 EDT 2021

Distinct Fibroblast Lineages Give Rise to NG2+ Pericyte Populations in Mouse Skin Development and Repair.

Frontiers in cell and developmental biology

Goss G,Rognoni E,Salameti V,Watt FM

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 62-1402-82) in Flow Cytometry

Wed Mar 02 00:00:00 EST 2022

Generation and characterization of a Myh6-driven Cre knockin mouse line.

Transgenic research

Huang X,Yan L,Kou S,Meng J,Lu Z,Lin CP,Liu C,Zhang H

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 62-1402-82) in Immunocytochemistry

Wed Dec 01 00:00:00 EST 2021

Direct contribution of skeletal muscle mesenchymal progenitors to bone repair.

Nature communications

Julien A,Kanagalingam A,Martínez-Sarrà E,Megret J,Luka M,Ménager M,Relaix F,Colnot C

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 62-1402-82) in Immunohistochemistry

Mon May 17 00:00:00 EDT 2021

Perivascular cell-derived extracellular vesicles stimulate colorectal cancer revascularization after withdrawal of antiangiogenic drugs.

Journal of extracellular vesicles

Huang M,Chen M,Qi M,Ye G,Pan J,Shi C,Yang Y,Zhao L,Mo X,Zhang Y,Li Y,Zhong J,Lu W,Li X,Zhang J,Lin J,Luo L,Liu T,Tang PM,Hong A,Cao Y,Ye W,Zhang D

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 62-1402-82) in Immunocytochemistry

Sat May 01 00:00:00 EDT 2021

Cardiac pericytes function as key vasoactive cells to regulate homeostasis and disease.

FEBS open bio

Lee LL,Khakoo AY,Chintalgattu V

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 62-1402-82) in Flow Cytometry

Fri Jan 01 00:00:00 EST 2021

Single-cell profiling reveals an endothelium-mediated immunomodulatory pathway in the eye choroid.

The Journal of experimental medicine

Lehmann GL,Hanke-Gogokhia C,Hu Y,Bareja R,Salfati Z,Ginsberg M,Nolan DJ,Mendez-Huergo SP,Dalotto-Moreno T,Wojcinski A,Ochoa F,Zeng S,Cerliani JP,Panagis L,Zager PJ,Mullins RF,Ogura S,Lutty GA,Bang J,Zippin JH,Romano C,Rabinovich GA,Elemento O,Joyner AL,Rafii S,Rodriguez-Boulan E,Benedicto I

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 62-1402-82) in Immunocytochemistry

Mon Jun 01 00:00:00 EDT 2020

TGFβ1 orchestrates renal fibrosis following Escherichia coli pyelonephritis.

Physiological reports

Hreha TN,Collins CA,Daugherty AL,Twentyman J,Paluri N,Hunstad DA

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 62-1402-82) in Immunohistochemistry

Sun Mar 01 00:00:00 EST 2020

Angiomodulin (IGFBP7) is a cerebral specific angiocrine factor, but is probably not a blood-brain barrier inducer.

Fluids and barriers of the CNS

Bar O,Gelb S,Atamny K,Anzi S,Ben-Zvi A

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 62-1402-82) in Immunocytochemistry

Wed Apr 01 00:00:00 EDT 2020

Angiomodulin (IGFBP7) is a cerebral specific angiocrine factor, but is probably not a blood-brain barrier inducer.

Fluids and barriers of the CNS

Bar O,Gelb S,Atamny K,Anzi S,Ben-Zvi A

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 62-1402-82) in Immunocytochemistry

Wed Apr 01 00:00:00 EDT 2020

Dual lineage tracing identifies intermediate mesenchymal stage for endocardial contribution to fibroblasts, coronary mural cells, and adipocytes.

The Journal of biological chemistry

Huang X,Feng T,Jiang Z,Meng J,Kou S,Lu Z,Chen W,Lin CP,Zhou B,Zhang H

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 62-1402-82) in Flow Cytometry

Fri May 31 00:00:00 EDT 2019

Dual lineage tracing identifies intermediate mesenchymal stage for endocardial contribution to fibroblasts, coronary mural cells, and adipocytes.

The Journal of biological chemistry

Huang X,Feng T,Jiang Z,Meng J,Kou S,Lu Z,Chen W,Lin CP,Zhou B,Zhang H

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 62-1402-82) in Flow Cytometry

Fri May 31 00:00:00 EDT 2019

Growth Factor Screening in Dystrophic Muscles Reveals PDGFB/PDGFRB-Mediated Migration of Interstitial Stem Cells.

International journal of molecular sciences

Camps J,Grosemans H,Gijsbers R,Maes C,Sampaolesi M

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 62-1402-82) in Flow Cytometry

Tue Mar 05 00:00:00 EST 2019

Pulmonary pericytes regulate lung morphogenesis.

Nature communications

Kato K,Diéguez-Hurtado R,Park DY,Hong SP,Kato-Azuma S,Adams S,Stehling M,Trappmann B,Wrana JL,Koh GY,Adams RH

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 62-1402-82) in Flow Cytometry

Fri Jun 22 00:00:00 EDT 2018

Repression of Interstitial Identity in Nephron Progenitor Cells by Pax2 Establishes the Nephron-Interstitium Boundary during Kidney Development.

Developmental cell

Naiman N,Fujioka K,Fujino M,Valerius MT,Potter SS,McMahon AP,Kobayashi A

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 62-1402-82) in Immunohistochemistry (Frozen)

Mon May 22 00:00:00 EDT 2017

Repression of Interstitial Identity in Nephron Progenitor Cells by Pax2 Establishes the Nephron-Interstitium Boundary during Kidney Development.

Developmental cell

Naiman N,Fujioka K,Fujino M,Valerius MT,Potter SS,McMahon AP,Kobayashi A

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 62-1402-82) in Immunohistochemistry (Frozen)

Mon May 22 00:00:00 EDT 2017

Pericytes regulate VEGF-induced endothelial sprouting through VEGFR1.

Nature communications

Eilken HM,Diéguez-Hurtado R,Schmidt I,Nakayama M,Jeong HW,Arf H,Adams S,Ferrara N,Adams RH

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 62-1402-82) in Immunohistochemistry

Fri Nov 17 00:00:00 EST 2017

Age-dependent modulation of vascular niches for haematopoietic stem cells.

Nature

Kusumbe AP,Ramasamy SK,Itkin T,Mäe MA,Langen UH,Betsholtz C,Lapidot T,Adams RH

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 62-1402-82) in Immunocytochemistry

Thu Apr 21 00:00:00 EDT 2016

The role of pericytic laminin in blood brain barrier integrity maintenance.

Scientific reports

Gautam J,Zhang X,Yao Y

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 62-1402-82) in Immunohistochemistry

Thu Nov 03 00:00:00 EDT 2016

Fibroblast Growth Factor 9 Imparts Hierarchy and Vasoreactivity to the Microcirculation of Renal Tumors and Suppresses Metastases.

The Journal of biological chemistry

Yin H,Frontini MJ,Arpino JM,Nong Z,O'Neil C,Xu Y,Balint B,Ward AD,Chakrabarti S,Ellis CG,Gros R,Pickering JG

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 62-1402-82) in Neutralization

Fri Sep 04 00:00:00 EDT 2015

Multiple stromal populations contribute to pulmonary fibrosis without evidence for epithelial to mesenchymal transition.

Proceedings of the National Academy of Sciences of the United States of America

Rock JR,Barkauskas CE,Cronce MJ,Xue Y,Harris JR,Liang J,Noble PW,Hogan BL

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 62-1402-82) in Immunocytochemistry

Tue Dec 27 00:00:00 EST 2011

Bioinformatics

Protein Aliases: Beta platelet-derived growth factor receptor; Beta-type platelet-derived growth factor receptor; CD140 antigen-like family member B; CD140b; CD140b antigen; PDGF beta chain; PDGF Receptor beta; PDGF-R-beta; PDGFR-1; PDGFR-beta; Platelet-derived growth factor receptor 1; Platelet-derived growth factor receptor beta; platelet-derived growth factor receptor beta variant 1

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Gene Aliases: AI528809; CD140b; Pdgfr; PDGFR-1; Pdgfr1; Pdgfrb

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UniProt ID: (Mouse) P05622

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Entrez Gene ID: (Mouse) 18596

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Function(s)

nucleotide binding protein kinase activity protein tyrosine kinase activity transmembrane receptor protein tyrosine kinase activity signal transducer activity platelet-derived growth factor-activated receptor activity platelet-derived growth factor beta-receptor activity receptor binding platelet-derived growth factor receptor binding protein binding ATP binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups protein kinase binding vascular endothelial growth factor binding phosphatidylinositol 3-kinase binding platelet-derived growth factor binding transmembrane signal receptor

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD140b (PDGFRB) Monoclonal Antibody (APB5), Super Bright™ 436, eBioscience™

CD140b (PDGFRB) Antibody (62-1402-82) in Flow

Product Details

62-1402-82

Immunohistochemistry (IHC)

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Neutralization (Neu)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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