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Invitrogen
CD140b (PDGFRB) Monoclonal Antibody (APB5), PE-eFluor™ 610, eBioscience™
FIGURE: 1 / 13
CD140b (PDGFRB) Antibody (61-1402-82) in Flow
Staining of the NIH/3T3 cell line with 0.25 µg of Rat IgG2a kappa Isotype Control, PE-eFluor 610 (Product # 61-4321-82) (blue histogram) or 0.25 µg of CD140b Monoclonal Antibody, PE-eFluor 610 (purple histogram). Total viable cells were used for analysis, as determined by Fixable Viability Dye eFluor 780 (Product # 65-0865-18).
Product Details
61-1402-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Not Applicable
Host/Isotype
Rat
/ IgG2a, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
APB5
Conjugate
Excitation/Emission Max
565/606 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2815310
Product Specific Information
Description: The APB5 monoclonal antibody reacts with the mouse CD140b molecule, the beta chain of the platelet derived growth factor receptor (PDGF receptor). PDGFRb is a receptor tyrosine kinase that forms dimers on the surface upon ligand binding and phosphorylates substrates. Dimers of PDGFR consist of either homodimers of alpha/alpha, beta/beta, or heterodimers of alpha/beta and serve as a substrate for its kinase activity. CD140b is expressed by embryonic tissues and mesenchymal-derived cells of the adult mouse tissues. The PDGFR beta chain is reported to play a significant role in formation of fibrous atherosclerotic lesions.
Applications Reported: This APB5 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This APB5 antibody has been tested by flow cytometric analysis of NIH/3T3 cells. This may be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
PE-eFluor 610 can be excited with laser lines from 488-561 nm and emits at 607 nm. We recommend using a 610/20 band pass filter (equivalent to PE-Texas Red). Please make sure that your instrument is capable of detecting this fluorochrome.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 488-561 nm; Emission: 607 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Target Information
PDGFRB is a receptor tyrosine kinase and has been implicated in atherogenesis and in cell transformation by the SIS oncogene. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. The gene is flanked on chromosome 5 by the genes for granulocyte-macrophage colony-stimulating factor and macrophage-colony stimulating factor receptor; all three genes may be implicated in the 5-q syndrome. A translocation between chromosomes 5 and 12, that fuses PDGFRB (CD140b) to that of the translocation, ETV6, leukemia gene, results in chronic myeloproliferative disorder with eosinophilia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Beta platelet-derived growth factor receptor; Beta-type platelet-derived growth factor receptor; CD140 antigen-like family member B; CD140b; CD140b antigen; PDGF beta chain; PDGF Receptor beta; PDGF-R-beta; PDGFR-1; PDGFR-beta; Platelet-derived growth factor receptor 1; Platelet-derived growth factor receptor beta; platelet-derived growth factor receptor beta variant 1
Gene Aliases: AI528809; CD140b; Pdgfr; PDGFR-1; Pdgfr1; Pdgfrb
UniProt ID: (Mouse) P05622
Entrez Gene ID: (Mouse) 18596
nucleotide binding
protein kinase activity
protein tyrosine kinase activity
transmembrane receptor protein tyrosine kinase activity
signal transducer activity
platelet-derived growth factor-activated receptor activity
platelet-derived growth factor beta-receptor activity
receptor binding
platelet-derived growth factor receptor binding
protein binding
ATP binding
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
protein kinase binding
vascular endothelial growth factor binding
phosphatidylinositol 3-kinase binding
platelet-derived growth factor binding
transmembrane signal receptor
in utero embryonic development
kidney development
tissue homeostasis
glycosaminoglycan biosynthetic process
protein phosphorylation
nitrogen compound metabolic process
chemotaxis
signal transduction
transmembrane receptor protein tyrosine kinase signaling pathway
multicellular organism development
positive regulation of cell proliferation
positive regulation of phospholipase C activity
positive regulation of phosphatidylinositol 3-kinase signaling
positive regulation of smooth muscle cell migration
phosphorylation
cell migration
peptidyl-tyrosine phosphorylation
hemopoiesis
adrenal gland development
positive regulation of cell migration
positive regulation of phosphoprotein phosphatase activity
regulation of actin cytoskeleton organization
positive regulation of collagen biosynthetic process
cell migration involved in vasculogenesis
intracellular signal transduction
metanephric mesenchymal cell migration
platelet-derived growth factor receptor-beta signaling pathway
positive regulation of metanephric mesenchymal cell migration by platelet-derived growth factor receptor-beta signaling pathway
aorta morphogenesis
cellular response to platelet-derived growth factor stimulus
positive regulation of cell proliferation by VEGF-activated platelet derived growth factor receptor signaling pathway
negative regulation of apoptotic process
positive regulation of MAP kinase activity
positive regulation of phosphatidylinositol 3-kinase activity
positive regulation of mitotic nuclear division
phosphatidylinositol metabolic process
protein autophosphorylation
platelet-derived growth factor receptor signaling pathway
phosphatidylinositol-mediated signaling
positive regulation of smooth muscle cell proliferation
skeletal system morphogenesis
smooth muscle tissue development
regulation of peptidyl-tyrosine phosphorylation
positive regulation of chemotaxis
cardiac myofibril assembly
cell chemotaxis
cardiac vascular smooth muscle cell differentiation
cell migration involved in coronary angiogenesis
retina vasculature development in camera-type eye
positive regulation of ERK1 and ERK2 cascade
smooth muscle cell chemotaxis
metanephric glomerular mesangial cell proliferation involved in metanephros development
metanephric glomerular capillary formation
positive regulation of calcium ion import
positive regulation of reactive oxygen species metabolic process
positive regulation of DNA biosynthetic process
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