Invitrogen

CD49b (Integrin alpha 2) Monoclonal Antibody (DX5), PE-Cyanine7, eBioscience™

4 Published Figures

25 References

View all (70) CD49b (Integrin alpha 2) antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (4)

CD49b (Integrin alpha 2) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 5

CD49b (Integrin alpha 2) Antibody (25-5971-82) in Flow

Staining of C57BL/6 splenocytes with Anti-Mouse NK1-1 FITC (Product # 11-5941-82) and staining buffer (autofluorescence) (left) or 0.25 µg of Anti-Mouse CD49b (Integrin alpha 2) PE-Cyanine7 (right). Total viable cells were used for analysis.

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Product Details

25-5971-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.5 µg/test

View 23 publications 23 publications

Functional Assay (Functional)

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgM, kappa

Class

Monoclonal

Type

Antibody

Clone

DX5

Conjugate

PE-Cyanine7 PE-Cyanine7 PE-Cyanine7

Excitation/Emission Max

569/780 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_469667

Product Specific Information

Description: The DX5 monoclonal antibody reacts with CD49b, an antigen expressed on a majority of mouse natural killer cells and a subset of T cells. DX5 reacts with all strains of mouse tested including the most commonly used strains, BALB/c, C57BL/6, C3H, CBA, DBA, AKR, SJL and 129. Simultaneous staining of C57BL/6 spleen cells with anti-NK1.1 mAb (PK136) and DX5 reveals coexpression of both markers by a majority of cells as well as presence of small populations of DX5+PK136- and DX5-PK136+ cells.

Applications Reported: This DX5 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This DX5 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD49b (Integrin alpha 2, Integrin alpha 2 chain, VLA-2 alpha chain, HM alpha 2) is a member of the integrin family. It is a glycoprotein with molecular weight of 150 kD, and it complexes with CD29 (Integrin beta 1) to form the heterodimeric integrin VLA-2 (integrin alpha 2 beta 1, or GPIa/IIa) complex. VLA-2 is an extracellular receptor for laminin, collagen, and fibronectin, and interaction with its ligands results in the activation of intracellular signaling pathways. It has reported roles in VEGF-induced angiogenesis in vivo, as well as adhesion and lymphocyte activation. CD49b is expressed by NK cells, NK-T cells, monocytes, platelets, and epithelial cells. It is also expressed on adaptive immune cells such as T and B cells, specifically on a subset of CD4+ T cells in the spleen, on intraepithelial and lamina propria lymphocytes in the intestine, as well as on a population of peripheral CD4+ type 1 T regulatory (Tr1) cells that co-express LAG-3.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Perinatal murine cytomegalovirus infection reshapes the transcriptional profile and functionality of NK cells.

Nature communications

Rožmanić C,Lisnić B,Pribanić Matešić M,Mihalić A,Hiršl L,Park E,Lesac Brizić A,Indenbirken D,Viduka I,Šantić M,Adler B,Yokoyama WM,Krmpotić A,Juranić Lisnić V,Jonjić S,Brizić I

Published figure using CD49b (Integrin alpha 2) monoclonal antibody (Product # 25-5971-82) in Flow Cytometry

Thu Oct 12 00:00:00 EDT 2023

Ultrasound combined with nanobubbles promotes systemic anticancer immunity and augments anti-PD1 efficacy.

Journal for immunotherapy of cancer

Hu J,He J,Wang Y,Zhao Y,Fang K,Dong Y,Chen Y,Zhang Y,Zhang C,Wang H,Tan J,Wang J,Zi R,Liu C,Liang H,Guo Y,Ou J

25-5971-82 was used in Flow Cytometry to provide a promising strategy for sensitizing poorly immunogenic solid tumors to immunotherapy in the clinic.

Tue Mar 01 00:00:00 EST 2022

Stage-specific action of Runx1 and GATA3 controls silencing of PU.1 expression in mouse pro-T cells.

The Journal of experimental medicine

Hosokawa H,Koizumi M,Masuhara K,Romero-Wolf M,Tanaka T,Nakayama T,Rothenberg EV

Published figure using CD49b (Integrin alpha 2) monoclonal antibody (Product # 25-5971-82) in Flow Cytometry

Mon Aug 02 00:00:00 EDT 2021

Francisella tularensis induces Th1 like MAIT cells conferring protection against systemic and local infection.

Nature communications

Zhao Z,Wang H,Shi M,Zhu T,Pediongco T,Lim XY,Meehan BS,Nelson AG,Fairlie DP,Mak JYW,Eckle SBG,de Lima Moreira M,Tumpach C,Bramhall M,Williams CG,Lee HJ,Haque A,Evrard M,Rossjohn J,McCluskey J,Corbett AJ,Chen Z

25-5971-82 was used in Flow Cytometry to highlight the potential utility of targeting MAIT cells to combat a range of bacterial pathogens.

Fri Jul 16 00:00:00 EDT 2021

Immunogenicity and protective efficacy of an intranasal live-attenuated vaccine against SARS-CoV-2.

iScience

Park JG,Oladunni FS,Rohaim MA,Whittingham-Dowd J,Tollitt J,Hodges MDJ,Fathallah N,Assas MB,Alhazmi W,Almilaibary A,Iqbal M,Chang P,Escalona R,Shivanna V,Torrelles JB,Worthington JJ,Jackson-Jones LH,Martinez-Sobrido L,Munir M

25-5971-82 was used in Flow Cytometry to evaluate a Newcastle disease virus (NDV)-based vectored-vaccine in mice and hamsters for its immunogenicity, safety and protective efficacy against SARS-CoV-2.

Fri Sep 24 00:00:00 EDT 2021

Potently Cytotoxic Natural Killer Cells Initially Emerge from Erythro-Myeloid Progenitors during Mammalian Development.

Developmental cell

Dege C,Fegan KH,Creamer JP,Berrien-Elliott MM,Luff SA,Kim D,Wagner JA,Kingsley PD,McGrath KE,Fehniger TA,Palis J,Sturgeon CM

25-5971 was used in Flow cytometry/Cell sorting to identify an extra-embryonic origin of potently cytotoxic NK cells, suggesting that ontogenic origin is a relevant factor in designing hPSC-derived adoptive immunotherapies.

Mon Apr 20 00:00:00 EDT 2020

IFN-Stimulated Gene 15 Is an Alarmin that Boosts the CTL Response via an Innate, NK Cell-Dependent Route.

Journal of immunology (Baltimore, Md. : 1950)

Iglesias-Guimarais V,Ahrends T,de Vries E,Knobeloch KP,Volkov A,Borst J

25-5971 was used in Flow cytometry/Cell sorting to conclude that free ISG15 is part of a newly recognized innate route to promote the CTL response.

Wed Apr 15 00:00:00 EDT 2020

Reduction of Global H3K27me³ Enhances HER2/ErbB2 Targeted Therapy.

Cell reports

Hirukawa A,Singh S,Wang J,Rennhack JP,Swiatnicki M,Sanguin-Gendreau V,Zuo D,Daldoul K,Lavoie C,Park M,Andrechek ER,Westbrook TF,Harris LN,Varadan V,Smith HW,Muller WJ

25-5971-82 was used in Flow cytometry/Cell sorting to provide evidence that EZH2 inhibitors promote interferon-driven immune responses that enhance the efficacy of anti-ErbB2 mAbs, suggesting the potential clinical benefit of epigenomic reprogramming by H3K27me3 depletion in Trastuzumab-resistant disease.

Tue Oct 08 00:00:00 EDT 2019

A Viral Immunoevasin Controls Innate Immunity by Targeting the Prototypical Natural Killer Cell Receptor Family.

Cell

Aguilar OA,Berry R,Rahim MMA,Reichel JJ,Popović B,Tanaka M,Fu Z,Balaji GR,Lau TNH,Tu MM,Kirkham CL,Mahmoud AB,Mesci A,Krmpotić A,Allan DSJ,Makrigiannis AP,Jonjić S,Rossjohn J,Carlyle JR

25-5971 was used in Flow cytometry/Cell sorting to identify a viral ligand for the inhibitory and activating NKR-P1 receptors.

Thu Mar 23 00:00:00 EDT 2017

The breast tumor microenvironment alters the phenotype and function of natural killer cells.

Cellular & molecular immunology

Krneta T,Gillgrass A,Chew M,Ashkar AA

Published figure using CD49b (Integrin alpha 2) monoclonal antibody (Product # 25-5971-82) in Flow Cytometry

Thu Sep 01 00:00:00 EDT 2016

NK-, NKT- and CD8-Derived IFNγ Drives Myeloid Cell Activation and Erythrophagocytosis, Resulting in Trypanosomosis-Associated Acute Anemia.

PLoS pathogens

Cnops J,De Trez C,Stijlemans B,Keirsse J,Kauffmann F,Barkhuizen M,Keeton R,Boon L,Brombacher F,Magez S

25-5971 was used in Flow cytometry/Cell sorting to show that NK, NKT and CD8+ T cell-derived IFNγ is a critical mediator in trypanosomosis-associated pathology.

Mon Jun 01 00:00:00 EDT 2015

VprBP Is Required for Efficient Editing and Selection of Igκ+ B Cells, but Is Dispensable for Igλ+ and Marginal Zone B Cell Maturation and Selection.

Journal of immunology (Baltimore, Md. : 1950)

Palmer VL,Aziz-Seible R,Kassmeier MD,Rothermund M,Perry GA,Swanson PC

25-5971 was used in Flow cytometry/Cell sorting to determine the contribution of the protein, VprBP, to B cell development and selection.

Sat Aug 15 00:00:00 EDT 2015

Genome Wide Identification of SARS-CoV Susceptibility Loci Using the Collaborative Cross.

PLoS genetics

Gralinski LE,Ferris MT,Aylor DL,Whitmore AC,Green R,Frieman MB,Deming D,Menachery VD,Miller DR,Buus RJ,Bell TA,Churchill GA,Threadgill DW,Katze MG,McMillan L,Valdar W,Heise MT,Pardo-Manuel de Villena F,Baric RS

25-5971 was used in Flow cytometry/Cell sorting to validate the Collaborative Cross platform as a resource for uncovering the genetic contributions of complex traits in microbial diseases, inflammation and viral replication.

Thu Oct 01 00:00:00 EDT 2015

Elimination of IL-10-inducing T-helper epitopes from an IGFBP-2 vaccine ensures potent antitumor activity.

Cancer research

Cecil DL,Holt GE,Park KH,Gad E,Rastetter L,Childs J,Higgins D,Disis ML

25-5971 was used in Flow cytometry/Cell sorting to investigate the deletion of immunosuppressive epitopes from an IGFBP-2 cancer vaccine as a novel strategy to increase antitumor activity.

Thu May 15 00:00:00 EDT 2014

Bcl11a controls Flt3 expression in early hematopoietic progenitors and is required for pDC development in vivo.

PloS one

Wu X,Satpathy AT,Kc W,Liu P,Murphy TL,Murphy KM

25-5971 was used in Flow cytometry/Cell sorting to investigate the role of Bcl11a in the development of dendritic cells, showing that it controls Flt3 expression in early hematopoietic progenitors and is required for pDC development.

Fri Jan 10 00:00:00 EST 2014

Galactose 6-O-sulfotransferases are not required for the generation of Siglec-F ligands in leukocytes or lung tissue.

The Journal of biological chemistry

Patnode ML,Cheng CW,Chou CC,Singer MS,Elin MS,Uchimura K,Crocker PR,Khoo KH,Rosen SD

25-5971 was used in Flow cytometry/Cell sorting to investigate whether keratan sulfate galactose 6-O-sulfotransferase and chondroitin 6-O-sulfotransferase 1 are required for the generation of endogenous Siglec-F ligands.

Fri Sep 13 00:00:00 EDT 2013

Phenotypic differences in virulence and immune response in closely related clinical isolates of influenza A 2009 H1N1 pandemic viruses in mice.

PloS one

Camp JV,Chu YK,Chung DH,McAllister RC,Adcock RS,Gerlach RL,Wiemken TL,Peyrani P,Ramirez JA,Summersgill JT,Jonsson CB

25-5971 was used in Flow cytometry/Cell sorting to capture the possible genotypic and phenotypic differences of the 2009 influenza A virus H1N1 pandemic strains circulating in adult hospitalized patients.

Tue Aug 13 00:00:00 EDT 2013

STAT1-independent control of a neurotropic measles virus challenge in primary neurons and infected mice.

Journal of immunology (Baltimore, Md. : 1950)

O'Donnell LA,Conway S,Rose RW,Nicolas E,Slifker M,Balachandran S,Rall GF

25-5971 was used in Flow cytometry/Cell sorting to determine whether STAT1 is required for IFN-γ-mediated protection from infection in neurons, showing that absence of STAT1 in mice did not restrict the ability of IFN-γ to control viral infection, through a pathway independent of the usual pathway.

Wed Feb 15 00:00:00 EST 2012

Type 2 innate immunity in helminth infection is induced redundantly and acts autonomously following CD11c(+) cell depletion.

Infection and immunity

Smith KA,Harcus Y,Garbi N,Hämmerling GJ,MacDonald AS,Maizels RM

25-5971 was used in Flow cytometry/Cell sorting to investigate the type 2 response to intestinal nematode parasites, showing that it is induced redundantly and acts autonomously following CD11c(+) cell depletion.

Mon Oct 01 00:00:00 EDT 2012

IL-10 regulates viral lung immunopathology during acute respiratory syncytial virus infection in mice.

PloS one

Loebbermann J,Schnoeller C,Thornton H,Durant L,Sweeney NP,Schuijs M,O'Garra A,Johansson C,Openshaw PJ

25-5971 was used in Flow cytometry/Cell sorting to study experimental respiratory syncytial virus infection in IL-10 deficient mice and mice treated with anti-IL-10 receptor antibody.

Mon Jul 23 00:00:00 EDT 2012

Divergent expression patterns of IL-4 and IL-13 define unique functions in allergic immunity.

Nature immunology

Liang HE,Reinhardt RL,Bando JK,Sullivan BM,Ho IC,Locksley RM

25-5971 was used in Flow cytometry/Cell sorting to assess the temporal and spatial production of IL-4 and IL-13 in vivo.

Sun Dec 04 00:00:00 EST 2011

CNS recruitment of CD8+ T lymphocytes specific for a peripheral virus infection triggers neuropathogenesis during polymicrobial challenge.

PLoS pathogens

Matullo CM,O'Regan KJ,Curtis M,Rall GF

25-5971 was used in Flow cytometry/Cell sorting to indicate that CD8+ T-cell recruitment to the brain can trigger CNS disease in the apparent absence of cognate antigen.

Thu Dec 01 00:00:00 EST 2011

Chronic helminth infection promotes immune regulation in vivo through dominance of CD11cloCD103- dendritic cells.

Journal of immunology (Baltimore, Md. : 1950)

Smith KA,Hochweller K,Hämmerling GJ,Boon L,MacDonald AS,Maizels RM

25-5971 was used in Magnetic cell separation to identify that the preferential expansion of a novel population of CD11c(lo) non-plasmacytoid DCs is associated with the pro-regulatory environment of chronic intestinal helminth infection.

Wed Jun 15 00:00:00 EDT 2011

Phenotypic differences between Th1 and Th17 cells and negative regulation of Th1 cell differentiation by IL-17.

Journal of leukocyte biology

Nakae S,Iwakura Y,Suto H,Galli SJ

25-5971 was used in Functional assays to compare the cell surface marker expression profiles of Th1 and Th17 cells.

Tue May 01 00:00:00 EDT 2007

A new approach for analyzing cellular infiltration during allergic airway inflammation.

Journal of immunological methods

Hoffmann PR,Gurary A,Hoffmann FW,Jourdan-Le Saux C,Teeters K,Hashimoto AC,Tam EK,Berry MJ

25-5971 was used in Flow cytometry/Cell sorting to investigate a new approach for analyzing cellular infiltration during allergic airway inflammation.

Sat Dec 01 00:00:00 EST 2007

Bioinformatics

Protein Aliases: BDPLT9; CD49 antigen-like family member B; CD49b; Collagen receptor; GPIa; Integrin alpha-2; Platelet membrane glycoprotein Ia; VLA-2 receptor, alpha 2 subunit; VLA-2 subunit alpha; VLA2 receptor

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Gene Aliases: CD49B; DX5; Itga2

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UniProt ID: (Mouse) Q62469

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Entrez Gene ID: (Mouse) 16398

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Function(s)

integrin binding collagen binding protein complex binding collagen receptor activity laminin binding metal ion binding protein heterodimerization activity collagen binding involved in cell-matrix adhesion integrin

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD49b (Integrin alpha 2) Monoclonal Antibody (DX5), PE-Cyanine7, eBioscience™

CD49b (Integrin alpha 2) Antibody (25-5971-82) in Flow

Product Details

25-5971-82

Flow Cytometry (Flow)

Functional Assay (Functional)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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