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Invitrogen
CD45 Monoclonal Antibody (30-F11), Brilliant Ultra Violet™ 563, eBioscience™
FIGURE: 1 / 1
CD45 Antibody (365-0451-82) in Flow
BALB/c mouse bone marrow cells were stained with TER-119 Monoclonal Antibody, APC (Product # 17-5921-82) and 0.25 µg of Rat IgG2b kappa Isotype Control, Brilliant Ultra Violet 563 (Product # 365-4031-81) (left) or 0.25 µg of CD45 Monoclonal Antibody, Brilliant Ultra Violet 563 (right). Total cells were used for analysis.
Product Details
365-0451-82
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rat
/ IgG2b, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
30-F11
Conjugate
Excitation/Emission Max
349/563 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with BSA
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2925377
Product Specific Information
Description: The 30-F11 monoclonal antibody reacts with all isoforms of mouse CD45, also known as Leukocyte Common Antigen (LCA). CD45 is expressed by all hematopoietic cells excluding mature erythrocytes and platelets. The cytoplasmic portion of CD45 has tyrosine phosphatase enzymatic activity and plays an important role in activation of lymphocytes.
Applications Reported: This 30-F11 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This 30-F11 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This may be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Brilliant Ultra Violet™ 563 (BUV563) is a tandem dye that emits at 564 nm and is intended for use on cytometers equipped with an ultraviolet (355 nm) laser. Please make sure that your instrument is capable of detecting this fluorochrome.
When using two or more Super Bright, Brilliant Violet™, Brilliant Ultra Violet™, or other polymer dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer or Brilliant Stain Buffer for more information.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone-specific performance should be determined empirically.
Excitation: 350 nm; Emission: 564 nm; Laser: Ultraviolet Laser.
BRILLIANT ULTRA VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, and is used under license. Powered by Sirigen™.
Target Information
CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase (PTP) ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 is absent on non-hematopoietic cell lines, normal and malignant, non-hematopoietic tissues. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing, differ in their extracellular domains but share identical transmembrane and cytoplasmic domains. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45 has been shown to be an essential regulator of T- and B-cell antigen receptor signaling and suppresses JAK kinases to regulate cytokine receptor signaling. CD45 is also important in promoting cell survival by modulating integrin-mediated signal transduction pathway, DNA fragmentation during apoptosis and inhibition or upregulation of various immunological functions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD45; L-CA; Leukocyte common antigen; Ly-5; Lymphocyte antigen 5; lymphocyte common antigen; Receptor-type tyrosine-protein phosphatase C; T200; T220 and B220
Gene Aliases: B220; Cd45; CD45R; L-CA; loc; Ly-5; Lyt-4; Ptprc; T200
UniProt ID: (Mouse) P06800
Entrez Gene ID: (Mouse) 19264
activation of MAPK activity
negative regulation of T cell mediated cytotoxicity
positive regulation of T cell mediated cytotoxicity
negative regulation of cytokine-mediated signaling pathway
hematopoietic progenitor cell differentiation
immunoglobulin biosynthetic process
positive regulation of T cell mediated immunity
regulation of humoral immune response mediated by circulating immunoglobulin
positive regulation of humoral immune response mediated by circulating immunoglobulin
negative regulation of protein kinase activity
protein dephosphorylation
negative regulation of cell adhesion involved in substrate-bound cell migration
leukocyte cell-cell adhesion
response to gamma radiation
dephosphorylation
B cell differentiation
T cell differentiation
positive regulation of B cell proliferation
negative regulation of protein autophosphorylation
heterotypic cell-cell adhesion
peptidyl-tyrosine dephosphorylation
T cell proliferation
B cell proliferation
positive regulation of T cell proliferation
positive regulation of MAPK cascade
cell cycle phase transition
positive thymic T cell selection
negative thymic T cell selection
regulation of B cell differentiation
positive regulation of T cell differentiation
positive regulation of gamma-delta T cell differentiation
positive regulation of alpha-beta T cell proliferation
positive regulation of isotype switching to IgG isotypes
bone marrow development
stem cell development
negative regulation of peptidyl-tyrosine phosphorylation
T cell receptor signaling pathway
B cell receptor signaling pathway
regulation of B cell receptor signaling pathway
positive regulation of antigen receptor-mediated signaling pathway
release of sequestered calcium ion into cytosol
defense response to virus
regulation of cell cycle
positive regulation of hematopoietic stem cell migration
positive regulation of stem cell proliferation
regulation of extrinsic apoptotic signaling pathway
positive regulation of extrinsic apoptotic signaling pathway
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