Invitrogen

IgM Monoclonal Antibody (II/41), PerCP-eFluor™ 710, eBioscience™

FIGURE: 1 / 32

IgM Antibody (46-5790-82) in Flow

Staining of C57BL/6 bone marrow cells with Anti-Human/Mouse CD45R (B220) APC (Product # 17-0452-82) and 0.25 µg of Rat IgG2a K Isotype Control PerCP-eFluor® 710 (Product # 46-4321-82) (left) or 0.25 µg of Anti-Mouse IgM PerCP-eFluor® 710 (right). Cells in the lymphocyte gate were used for analysis.

Product Image Gallery

Product Details

46-5790-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.5 µg/test

View 27 publications 27 publications

Product Specifications

Species Reactivity

Mouse

Published species

Fish, Human, Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), PerCP-eFluor™ 710, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

II/41

Conjugate

PerCP-eFluor™ 710 PerCP-eFluor™ 710 PerCP-eFluor™ 710

Excitation/Emission Max

482/708 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1834435

Product Specific Information

Description: The II/41 monoclonal antibody reacts with the µ heavy chain of mouse IgM. It does not react with other classes of mouse immunoglobulin including IgD, IgG or IgA. IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells. Fluorochrome conjugated II/41 can be used as a detection secondary for mouse IgM.

Applications Reported: This II/41 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This II/41 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

PerCP-eFluor® 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm). Please make sure that your instrument is capable of detecting this fluorochrome. For a filter configuration, we recommend using the 685 LP dichroic mirror and 710/40 band pass filter, however the 695/40 band pass filter is an acceptable alternative.

Our testing indicates that PerCP-eFluor® 710 conjugated antibodies are stable when stained samples are exposed to freshly prepared 2% formaldehyde overnight at 4°C, but please evaluate for alternative fixation protocols.

Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Mouse IgM is an immunoglobulin M antibody that is produced by B cells in response to an antigen. It is the first antibody produced during an immune response and is the largest immunoglobulin in the serum. Mouse IgM is composed of five heavy chains and five light chains, and it is involved in complement activation and agglutination of antigens. It plays a crucial role in the innate immune response and is important for neutralizing pathogens before the adaptive immune response is fully activated. Mouse IgM is widely used in research and diagnostic applications, including ELISA, Western blotting, and immunohistochemistry.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

B cell class switch recombination is regulated by DYRK1A through MSH6 phosphorylation.

Nature communications

Stoler-Barak L,Harris E,Peres A,Hezroni H,Kuka M,Di Lucia P,Grenov A,Gurwicz N,Kupervaser M,Yip BH,Iannacone M,Yaari G,Crispino JD,Shulman Z

46-5790-82 was used in Flow cytometry/Cell sorting to demonstrate DYRK1A-mediated biological mechanisms of B cell immune responses that may be used for therapeutic manipulation in antibody-mediated autoimmunity.

Thu Mar 16 00:00:00 EDT 2023

Nanobodies from camelid mice and llamas neutralize SARS-CoV-2 variants.

Nature

Xu J,Xu K,Jung S,Conte A,Lieberman J,Muecksch F,Lorenzi JCC,Park S,Schmidt F,Wang Z,Huang Y,Luo Y,Nair MS,Wang P,Schulz JE,Tessarollo L,Bylund T,Chuang GY,Olia AS,Stephens T,Teng IT,Tsybovsky Y,Zhou T,Munster V,Ho DD,Hatziioannou T,Bieniasz PD,Nussenzweig MC,Kwong PD,Casellas R

Published figure using IgM monoclonal antibody (Product # 46-5790-82) in Flow Cytometry

Thu Jul 01 00:00:00 EDT 2021

Smc3 dosage regulates B cell transit through germinal centers and restricts their malignant transformation.

Nature immunology

Rivas MA,Meydan C,Chin CR,Challman MF,Kim D,Bhinder B,Kloetgen A,Viny AD,Teater MR,McNally DR,Doane AS,Béguelin W,Fernández MTC,Shen H,Wang X,Levine RL,Chen Z,Tsirigos A,Elemento O,Mason CE,Melnick AM

Published figure using IgM monoclonal antibody (Product # 46-5790-82) in Flow Cytometry

Mon Feb 01 00:00:00 EST 2021

A platform-agnostic, function first-based antibody discovery strategy using plasmid-free mammalian expression of antibodies.

mAbs

Zhang R,Prabakaran P,Yu X,Mackness BC,Boudanova E,Hopke J,Sancho J,Saleh J,Cho H,Zhang N,Simonds-Mannes H,Stimple SD,Hoffmann D,Park A,Chowdhury PS,Rao SP

Published figure using IgM monoclonal antibody (Product # 46-5790-82) in Flow Cytometry

Wed Jan 19 00:00:00 EST 2022

Construction, characterization, and immunization of nanoparticles that display a diverse array of influenza HA trimers.

PloS one

Cohen AA,Yang Z,Gnanapragasam PNP,Ou S,Dam KA,Wang H,Bjorkman PJ

46-5790-82 was used in Flow Cytometry to demonstrate homotypic and heterotypic conjugation of 8 different hemagglutinin trimers.

Fri Oct 15 00:00:00 EDT 2021

Construction, characterization, and immunization of nanoparticles that display a diverse array of influenza HA trimers.

PloS one

Cohen AA,Yang Z,Gnanapragasam PNP,Ou S,Dam KA,Wang H,Bjorkman PJ

46-5790-82 was used in Flow Cytometry to demonstrate homotypic and heterotypic conjugation of 8 different hemagglutinin trimers.

Fri Oct 15 00:00:00 EDT 2021

B Cell Diversification Is Uncoupled from SAP-Mediated Selection Forces in Chronic Germinal Centers within Peyer's Patches.

Cell reports

Biram A,Winter E,Denton AE,Zaretsky I,Dassa B,Bemark M,Linterman MA,Yaari G,Shulman Z

46-5790-82 was used in Flow cytometry/Cell sorting to demonstrate that T cell-derived help to B cells in PPs includes SAP-dependent and SAP-independent functions.

Tue Feb 11 00:00:00 EST 2020

B Cell Diversification Is Uncoupled from SAP-Mediated Selection Forces in Chronic Germinal Centers within Peyer's Patches.

Cell reports

Biram A,Winter E,Denton AE,Zaretsky I,Dassa B,Bemark M,Linterman MA,Yaari G,Shulman Z

46-5790-82 was used in Flow cytometry/Cell sorting to demonstrate that T cell-derived help to B cells in PPs includes SAP-dependent and SAP-independent functions.

Tue Feb 11 00:00:00 EST 2020

Chronic Viral Infection Promotes Efficient Germinal Center B Cell Responses.

Cell reports

Fallet B,Hao Y,Florova M,Cornille K,de Los Aires AV,Girelli Zubani G,Ertuna YI,Greiff V,Menzel U,Hammad K,Merkler D,Reddy ST,Weill JC,Reynaud CA,Pinschewer DD

Published figure using IgM monoclonal antibody (Product # 46-5790-82) in Flow Cytometry

Tue Jan 28 00:00:00 EST 2020

TET enzymes control antibody production and shape the mutational landscape in germinal centre B cells.

The FEBS journal

Schoeler K,Aufschnaiter A,Messner S,Derudder E,Herzog S,Villunger A,Rajewsky K,Labi V

Published figure using IgM monoclonal antibody (Product # 46-5790-82) in Flow Cytometry

Sun Sep 01 00:00:00 EDT 2019

A protocol for generating induced T cells by reprogramming B cells in vivo.

Cell regeneration (London, England)

Weng Q,Hu F,Zhang M,Dong Y,Lv C,Wang Y,Liu X,Wang J

Published figure using IgM monoclonal antibody (Product # 46-5790-82) in Flow Cytometry

Sat Sep 01 00:00:00 EDT 2018

mTOR has distinct functions in generating versus sustaining humoral immunity.

The Journal of clinical investigation

Jones DD,Gaudette BT,Wilmore JR,Chernova I,Bortnick A,Weiss BM,Allman D

46-5790 was used in Flow cytometry/Cell sorting to conclude that mTOR signalling plays critical but diverse roles in early and late phases of antibody responses and plasma cell differentiation.

Tue Nov 01 00:00:00 EDT 2016

Conversion of T cells to B cells by inactivation of polycomb-mediated epigenetic suppression of the B-lineage program.

Genes & development

Ikawa T,Masuda K,Endo TA,Endo M,Isono K,Koseki Y,Nakagawa R,Kometani K,Takano J,Agata Y,Katsura Y,Kurosaki T,Vidal M,Koseki H,Kawamoto H

Published figure using IgM monoclonal antibody (Product # 46-5790-82) in Flow Cytometry

Tue Nov 15 00:00:00 EST 2016

Ligase I and ligase III mediate the DNA double-strand break ligation in alternative end-joining.

Proceedings of the National Academy of Sciences of the United States of America

Lu G,Duan J,Shu S,Wang X,Gao L,Guo J,Zhang Y

Published figure using IgM monoclonal antibody (Product # 46-5790-82) in Flow Cytometry

Tue Feb 02 00:00:00 EST 2016

Editing of mouse and human immunoglobulin genes by CRISPR-Cas9 system.

Nature communications

Cheong TC,Compagno M,Chiarle R

Published figure using IgM monoclonal antibody (Product # 46-5790-82) in Flow Cytometry

Wed Mar 09 00:00:00 EST 2016

Sequencing and cloning of antigen-specific antibodies from mouse memory B cells.

Nature protocols

von Boehmer L,Liu C,Ackerman S,Gitlin AD,Wang Q,Gazumyan A,Nussenzweig MC

46-5790-82 was used in Flow cytometry/Cell sorting to sequence and clone the variable antibody region of single antigen-specific mouse memory B cells for antibody production.

Sat Oct 01 00:00:00 EDT 2016

B cell autophagy mediates TLR7-dependent autoimmunity and inflammation.

Autophagy

Weindel CG,Richey LJ,Bolland S,Mehta AJ,Kearney JF,Huber BT

Published figure using IgM monoclonal antibody (Product # 46-5790-82) in Flow Cytometry

Mon Apr 18 00:00:00 EDT 2016

Mitochondrial ATP transporter Ant2 depletion impairs erythropoiesis and B lymphopoiesis.

Cell death and differentiation

Cho J,Seo J,Lim CH,Yang L,Shiratsuchi T,Lee MH,Chowdhury RR,Kasahara H,Kim JS,Oh SP,Lee YJ,Terada N

Published figure using IgM monoclonal antibody (Product # 46-5790-82) in Flow Cytometry

Tue Sep 01 00:00:00 EDT 2015

Cellular Dynamics of Memory B Cell Populations: IgM+ and IgG+ Memory B Cells Persist Indefinitely as Quiescent Cells.

Journal of immunology (Baltimore, Md. : 1950)

Jones DD,Wilmore JR,Allman D

46-5790 was used in Flow cytometry/Cell sorting to demonstrate that once established, both IgG-positive and less frequent IgM-positive memory populations are exceptionally stable, with little evidence of attrition or cellular turnover.

Sun Nov 15 00:00:00 EST 2015

Elucidation of the enigmatic IgD class-switch recombination via germline deletion of the IgH 3' regulatory region.

The Journal of experimental medicine

Rouaud P,Saintamand A,Saad F,Carrion C,Lecardeur S,Cogné M,Denizot Y

Published figure using IgM monoclonal antibody (Product # 46-5790-82) in Flow Cytometry

Mon May 05 00:00:00 EDT 2014

Type I interferon signaling enhances CD8+ T cell effector function and differentiation during murine gammaherpesvirus 68 infection.

Journal of virology

Jennings RN,Grayson JM,Barton ES

46-5790 was used in Flow cytometry/Cell sorting to assess T cell responses in IFNAR1(-/-) mice after MHV68 infection.

Mon Dec 01 00:00:00 EST 2014

A component of the mir-17-92 polycistronic oncomir promotes oncogene-dependent apoptosis.

eLife

Olive V,Sabio E,Bennett MJ,De Jong CS,Biton A,McGann JC,Greaney SK,Sodir NM,Zhou AY,Balakrishnan A,Foth M,Luftig MA,Goga A,Speed TP,Xuan Z,Evan GI,Wan Y,Minella AC,He L

Published figure using IgM monoclonal antibody (Product # 46-5790-82) in Flow Cytometry

Tue Oct 15 00:00:00 EDT 2013

A mouse model for inducible overexpression of Prdm14 results in rapid-onset and highly penetrant T-cell acute lymphoblastic leukemia (T-ALL).

Disease models & mechanisms

Carofino BL,Ayanga B,Justice MJ

Published figure using IgM monoclonal antibody (Product # 46-5790-82) in Flow Cytometry

Fri Nov 01 00:00:00 EDT 2013

Nucleoside salvage pathway kinases regulate hematopoiesis by linking nucleotide metabolism with replication stress.

The Journal of experimental medicine

Austin WR,Armijo AL,Campbell DO,Singh AS,Hsieh T,Nathanson D,Herschman HR,Phelps ME,Witte ON,Czernin J,Radu CG

46-5790 was used in Flow cytometry/Cell sorting to investigate a functional link between nucleotide deficiency, replication stress, and two enzymes of the nucleoside salvage pathway: deoxycytidine kinase and thymidine kinase.

Mon Nov 19 00:00:00 EST 2012

Critical role of B cell lymphoma 10 in BAFF-regulated NF-κB activation and survival of anergic B cells.

Journal of immunology (Baltimore, Md. : 1950)

Yu M,Chen Y,He Y,Podd A,Fu G,Wright JA,Kleiman E,Khan WN,Wen R,Wang D

Published figure using IgM monoclonal antibody (Product # 46-5790-82) in Flow Cytometry

Sat Dec 01 00:00:00 EST 2012

Nuclear export of the NF-κB inhibitor IκBα is required for proper B cell and secondary lymphoid tissue formation.

Immunity

Wuerzberger-Davis SM,Chen Y,Yang DT,Kearns JD,Bates PW,Lynch C,Ladell NC,Yu M,Podd A,Zeng H,Huang TT,Wen R,Hoffmann A,Wang D,Miyamoto S

Published figure using IgM monoclonal antibody (Product # 46-5790-82) in Flow Cytometry

Fri Feb 25 00:00:00 EST 2011

A critical role of TAK1 in B-cell receptor-mediated nuclear factor kappaB activation.

Blood

Schuman J,Chen Y,Podd A,Yu M,Liu HH,Wen R,Chen ZJ,Wang D

Published figure using IgM monoclonal antibody (Product # 46-5790-82) in Flow Cytometry

Thu May 07 00:00:00 EDT 2009

miR-19 is a key oncogenic component of mir-17-92.

Genes & development

Olive V,Bennett MJ,Walker JC,Ma C,Jiang I,Cordon-Cardo C,Li QJ,Lowe SW,Hannon GJ,He L

Published figure using IgM monoclonal antibody (Product # 46-5790-82) in Flow Cytometry

Tue Dec 15 00:00:00 EST 2009

Phospholipase Cgamma2 contributes to light-chain gene activation and receptor editing.

Molecular and cellular biology

Bai L,Chen Y,He Y,Dai X,Lin X,Wen R,Wang D

Published figure using IgM monoclonal antibody (Product # 46-5790-82) in Flow Cytometry

Sat Sep 01 00:00:00 EDT 2007

Bioinformatics

Gene Aliases: AI326478; Igh-6; Igh-M; Igh6; Igm; muH

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Entrez Gene ID: (Mouse) 16019

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Function(s)

immunoglobulin

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

IgM Monoclonal Antibody (II/41), PerCP-eFluor™ 710, eBioscience™

IgM Antibody (46-5790-82) in Flow

Product Details

46-5790-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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