Invitrogen

IgM Monoclonal Antibody (II/41), eFluor™ 660, eBioscience™

38 Published Figures

33 References

View all (30) Mouse IgM antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (2)
Published Figures (38)

IgM Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 40

IgM Antibody (50-5790-82) in ICC/IF

Immunocytochemistry of fixed, differentiated OLN93 cells using 10 µg/mL of Anti-Oligodendrocyte Marker O1 Purified (Product # 14-6506-82) followed by 10 µg/mL of Anti-Mouse IgM eFluor® 660. Nuclei are stained with DAPI.

IgM Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)

IgM Antibody in Immunohistochemistry, Flow Cytometry (IHC, Flow)

IgM Antibody in Immunohistochemistry (IHC)

IgM Antibody in Flow Cytometry, ELISA (Flow, ELISA)

Product Image Gallery

Product Details

50-5790-82

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

10 µg/mL

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

10 µg/mL

View 2 publications 2 publications

Flow Cytometry (Flow)

0.25 µg/test

View 22 publications 22 publications

ELISA (ELISA)

View 5 publications 5 publications

T-Cell Activation (TCA)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Fish, Human, Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), eFluor™ 660, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

II/41

Conjugate

eFluor™ 660 eFluor™ 660 eFluor™ 660

Excitation/Emission Max

651/668 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2574246

Product Specific Information

Description: The II/41 monoclonal antibody reacts with the mu heavy chain of mouse IgM. It does not react with other classes of mouse immunoglobulin including IgD, IgG or IgA. IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells. Fluorochrome conjugated II/41 can be used as a detection secondary for mouse IgM.

Applications Reported: This II/41 antibody has been reported for use in flow cytometric analysis, microscopy, immunohistochemical staining, and immunocytochemistry.

Applications Tested: This II/41 antibody has been tested by immunocytochemistry on fixed cells and by immunohistochemistry on formalin-fixed paraffin embedded sections that were stained first with a primary antibody of the mouse IgM isotype. For immunocytochemistry and immunohistochemistry, this can be used at less than or equal to 10 µg/mL. This II/41 antibody has also been tested by flow cytometric analysis of mouse bone marrow cells and can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody by carefully titrated for optimal performance in the assay of interest.

eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Mouse IgM is an immunoglobulin M antibody that is produced by B cells in response to an antigen. It is the first antibody produced during an immune response and is the largest immunoglobulin in the serum. Mouse IgM is composed of five heavy chains and five light chains, and it is involved in complement activation and agglutination of antigens. It plays a crucial role in the innate immune response and is important for neutralizing pathogens before the adaptive immune response is fully activated. Mouse IgM is widely used in research and diagnostic applications, including ELISA, Western blotting, and immunohistochemistry.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Rad52 mediates class-switch DNA recombination to IgD.

Nature communications

Xu Y,Zhou H,Post G,Zan H,Casali P

Published figure using IgM monoclonal antibody (Product # 50-5790-82) in Immunohistochemistry

Mon Feb 21 00:00:00 EST 2022

Nanobodies from camelid mice and llamas neutralize SARS-CoV-2 variants.

Nature

Xu J,Xu K,Jung S,Conte A,Lieberman J,Muecksch F,Lorenzi JCC,Park S,Schmidt F,Wang Z,Huang Y,Luo Y,Nair MS,Wang P,Schulz JE,Tessarollo L,Bylund T,Chuang GY,Olia AS,Stephens T,Teng IT,Tsybovsky Y,Zhou T,Munster V,Ho DD,Hatziioannou T,Bieniasz PD,Nussenzweig MC,Kwong PD,Casellas R

Published figure using IgM monoclonal antibody (Product # 50-5790-82) in Flow Cytometry

Thu Jul 01 00:00:00 EDT 2021

Smc3 dosage regulates B cell transit through germinal centers and restricts their malignant transformation.

Nature immunology

Rivas MA,Meydan C,Chin CR,Challman MF,Kim D,Bhinder B,Kloetgen A,Viny AD,Teater MR,McNally DR,Doane AS,Béguelin W,Fernández MTC,Shen H,Wang X,Levine RL,Chen Z,Tsirigos A,Elemento O,Mason CE,Melnick AM

Published figure using IgM monoclonal antibody (Product # 50-5790-82) in Flow Cytometry

Mon Feb 01 00:00:00 EST 2021

A platform-agnostic, function first-based antibody discovery strategy using plasmid-free mammalian expression of antibodies.

mAbs

Zhang R,Prabakaran P,Yu X,Mackness BC,Boudanova E,Hopke J,Sancho J,Saleh J,Cho H,Zhang N,Simonds-Mannes H,Stimple SD,Hoffmann D,Park A,Chowdhury PS,Rao SP

Published figure using IgM monoclonal antibody (Product # 50-5790-82) in Flow Cytometry

Wed Jan 19 00:00:00 EST 2022

Stearoyl-CoA Desaturase-Mediated Monounsaturated Fatty Acid Availability Supports Humoral Immunity.

Cell reports

Zhou X,Zhu X,Li C,Li Y,Ye Z,Shapiro VS,Copland JA,Hitosugi T,Bernlohr DA,Sun J,Zeng H

Published figure using IgM monoclonal antibody (Product # 50-5790-82) in ELISA

Tue Jan 05 00:00:00 EST 2021

Construction, characterization, and immunization of nanoparticles that display a diverse array of influenza HA trimers.

PloS one

Cohen AA,Yang Z,Gnanapragasam PNP,Ou S,Dam KA,Wang H,Bjorkman PJ

Published figure using IgM monoclonal antibody (Product # 50-5790-82) in Flow Cytometry

Fri Oct 15 00:00:00 EDT 2021

B Cell Diversification Is Uncoupled from SAP-Mediated Selection Forces in Chronic Germinal Centers within Peyer's Patches.

Cell reports

Biram A,Winter E,Denton AE,Zaretsky I,Dassa B,Bemark M,Linterman MA,Yaari G,Shulman Z

Published figure using IgM monoclonal antibody (Product # 50-5790-82) in Flow Cytometry

Tue Feb 11 00:00:00 EST 2020

Chronic Viral Infection Promotes Efficient Germinal Center B Cell Responses.

Cell reports

Fallet B,Hao Y,Florova M,Cornille K,de Los Aires AV,Girelli Zubani G,Ertuna YI,Greiff V,Menzel U,Hammad K,Merkler D,Reddy ST,Weill JC,Reynaud CA,Pinschewer DD

Published figure using IgM monoclonal antibody (Product # 50-5790-82) in Flow Cytometry

Tue Jan 28 00:00:00 EST 2020

TET enzymes control antibody production and shape the mutational landscape in germinal centre B cells.

The FEBS journal

Schoeler K,Aufschnaiter A,Messner S,Derudder E,Herzog S,Villunger A,Rajewsky K,Labi V

Published figure using IgM monoclonal antibody (Product # 50-5790-82) in Flow Cytometry

Sun Sep 01 00:00:00 EDT 2019

Distinct Requirements of CHD4 during B Cell Development and Antibody Response.

Cell reports

Yen WF,Sharma R,Cols M,Lau CM,Chaudhry A,Chowdhury P,Yewdell WT,Vaidyanathan B,Sun A,Coffre M,Pucella JN,Chen CC,Jasin M,Sun JC,Rudensky AY,Koralov SB,Chaudhuri J

Published figure using IgM monoclonal antibody (Product # 50-5790-82) in ELISA

Tue Apr 30 00:00:00 EDT 2019

Anti‑IL‑39 (IL‑23p19/Ebi3) polyclonal antibodies ameliorate autoimmune symptoms in lupus‑like mice.

Molecular medicine reports

Wang X,Zhang Y,Wang Z,Liu X,Zhu G,Han G,Chen G,Hou C,Wang T,Shen B,Li Y,Xiao H,Ma N,Wang R

Published figure using IgM monoclonal antibody (Product # 50-5790-82) in ELISA

Mon Jan 01 00:00:00 EST 2018

A protocol for generating induced T cells by reprogramming B cells in vivo.

Cell regeneration (London, England)

Weng Q,Hu F,Zhang M,Dong Y,Lv C,Wang Y,Liu X,Wang J

Published figure using IgM monoclonal antibody (Product # 50-5790-82) in Flow Cytometry

Sat Sep 01 00:00:00 EDT 2018

PI3Kδ hyper-activation promotes development of B cells that exacerbate Streptococcus pneumoniae infection in an antibody-independent manner.

Nature communications

Stark AK,Chandra A,Chakraborty K,Alam R,Carbonaro V,Clark J,Sriskantharajah S,Bradley G,Richter AG,Banham-Hall E,Clatworthy MR,Nejentsev S,Hamblin JN,Hessel EM,Condliffe AM,Okkenhaug K

Published figure using IgM monoclonal antibody (Product # 50-5790-82) in T-Cell Activation

Thu Aug 09 00:00:00 EDT 2018

Molecular requirements of the B-cell antigen receptor for sensing monovalent antigens.

The EMBO journal

Volkmann C,Brings N,Becker M,Hobeika E,Yang J,Reth M

50-5790 was used in Flow cytometry/Cell sorting to study the effects of differing monovalent stimuli on B-cell antigen receptor signalling and organisation.

Wed Nov 02 00:00:00 EDT 2016

Conversion of T cells to B cells by inactivation of polycomb-mediated epigenetic suppression of the B-lineage program.

Genes & development

Ikawa T,Masuda K,Endo TA,Endo M,Isono K,Koseki Y,Nakagawa R,Kometani K,Takano J,Agata Y,Katsura Y,Kurosaki T,Vidal M,Koseki H,Kawamoto H

Published figure using IgM monoclonal antibody (Product # 50-5790-82) in ELISA

Tue Nov 15 00:00:00 EST 2016

Conversion of T cells to B cells by inactivation of polycomb-mediated epigenetic suppression of the B-lineage program.

Genes & development

Ikawa T,Masuda K,Endo TA,Endo M,Isono K,Koseki Y,Nakagawa R,Kometani K,Takano J,Agata Y,Katsura Y,Kurosaki T,Vidal M,Koseki H,Kawamoto H

Published figure using IgM monoclonal antibody (Product # 50-5790-82) in ELISA

Tue Nov 15 00:00:00 EST 2016

Ligase I and ligase III mediate the DNA double-strand break ligation in alternative end-joining.

Proceedings of the National Academy of Sciences of the United States of America

Lu G,Duan J,Shu S,Wang X,Gao L,Guo J,Zhang Y

Published figure using IgM monoclonal antibody (Product # 50-5790-82) in Flow Cytometry

Tue Feb 02 00:00:00 EST 2016

Editing of mouse and human immunoglobulin genes by CRISPR-Cas9 system.

Nature communications

Cheong TC,Compagno M,Chiarle R

Published figure using IgM monoclonal antibody (Product # 50-5790-82) in Flow Cytometry

Wed Mar 09 00:00:00 EST 2016

B cell autophagy mediates TLR7-dependent autoimmunity and inflammation.

Autophagy

Weindel CG,Richey LJ,Bolland S,Mehta AJ,Kearney JF,Huber BT

Published figure using IgM monoclonal antibody (Product # 50-5790-82) in ELISA

Mon Apr 18 00:00:00 EDT 2016

B cell autophagy mediates TLR7-dependent autoimmunity and inflammation.

Autophagy

Weindel CG,Richey LJ,Bolland S,Mehta AJ,Kearney JF,Huber BT

Published figure using IgM monoclonal antibody (Product # 50-5790-82) in ELISA

Mon Apr 18 00:00:00 EDT 2016

B cell autophagy mediates TLR7-dependent autoimmunity and inflammation.

Autophagy

Weindel CG,Richey LJ,Bolland S,Mehta AJ,Kearney JF,Huber BT

Published figure using IgM monoclonal antibody (Product # 50-5790-82) in ELISA

Mon Apr 18 00:00:00 EDT 2016

Mitochondrial ATP transporter Ant2 depletion impairs erythropoiesis and B lymphopoiesis.

Cell death and differentiation

Cho J,Seo J,Lim CH,Yang L,Shiratsuchi T,Lee MH,Chowdhury RR,Kasahara H,Kim JS,Oh SP,Lee YJ,Terada N

Published figure using IgM monoclonal antibody (Product # 50-5790-82) in Flow Cytometry

Tue Sep 01 00:00:00 EDT 2015

Elucidation of the enigmatic IgD class-switch recombination via germline deletion of the IgH 3' regulatory region.

The Journal of experimental medicine

Rouaud P,Saintamand A,Saad F,Carrion C,Lecardeur S,Cogné M,Denizot Y

Published figure using IgM monoclonal antibody (Product # 50-5790-82) in Flow Cytometry

Mon May 05 00:00:00 EDT 2014

Elucidation of the enigmatic IgD class-switch recombination via germline deletion of the IgH 3' regulatory region.

The Journal of experimental medicine

Rouaud P,Saintamand A,Saad F,Carrion C,Lecardeur S,Cogné M,Denizot Y

Published figure using IgM monoclonal antibody (Product # 50-5790-82) in Flow Cytometry

Mon May 05 00:00:00 EDT 2014

A component of the mir-17-92 polycistronic oncomir promotes oncogene-dependent apoptosis.

eLife

Olive V,Sabio E,Bennett MJ,De Jong CS,Biton A,McGann JC,Greaney SK,Sodir NM,Zhou AY,Balakrishnan A,Foth M,Luftig MA,Goga A,Speed TP,Xuan Z,Evan GI,Wan Y,Minella AC,He L

Published figure using IgM monoclonal antibody (Product # 50-5790-82) in Flow Cytometry

Tue Oct 15 00:00:00 EDT 2013

A mouse model for inducible overexpression of Prdm14 results in rapid-onset and highly penetrant T-cell acute lymphoblastic leukemia (T-ALL).

Disease models & mechanisms

Carofino BL,Ayanga B,Justice MJ

Published figure using IgM monoclonal antibody (Product # 50-5790-82) in Flow Cytometry

Fri Nov 01 00:00:00 EDT 2013

Critical role of B cell lymphoma 10 in BAFF-regulated NF-κB activation and survival of anergic B cells.

Journal of immunology (Baltimore, Md. : 1950)

Yu M,Chen Y,He Y,Podd A,Fu G,Wright JA,Kleiman E,Khan WN,Wen R,Wang D

Published figure using IgM monoclonal antibody (Product # 50-5790-82) in Flow Cytometry

Sat Dec 01 00:00:00 EST 2012

Nuclear export of the NF-κB inhibitor IκBα is required for proper B cell and secondary lymphoid tissue formation.

Immunity

Wuerzberger-Davis SM,Chen Y,Yang DT,Kearns JD,Bates PW,Lynch C,Ladell NC,Yu M,Podd A,Zeng H,Huang TT,Wen R,Hoffmann A,Wang D,Miyamoto S

Published figure using IgM monoclonal antibody (Product # 50-5790-82) in Flow Cytometry

Fri Feb 25 00:00:00 EST 2011

Nuclear export of the NF-κB inhibitor IκBα is required for proper B cell and secondary lymphoid tissue formation.

Immunity

Wuerzberger-Davis SM,Chen Y,Yang DT,Kearns JD,Bates PW,Lynch C,Ladell NC,Yu M,Podd A,Zeng H,Huang TT,Wen R,Hoffmann A,Wang D,Miyamoto S

Published figure using IgM monoclonal antibody (Product # 50-5790-82) in Flow Cytometry

Fri Feb 25 00:00:00 EST 2011

A critical role of TAK1 in B-cell receptor-mediated nuclear factor kappaB activation.

Blood

Schuman J,Chen Y,Podd A,Yu M,Liu HH,Wen R,Chen ZJ,Wang D

Published figure using IgM monoclonal antibody (Product # 50-5790-82) in Immunofluorescence

Thu May 07 00:00:00 EDT 2009

A critical role of TAK1 in B-cell receptor-mediated nuclear factor kappaB activation.

Blood

Schuman J,Chen Y,Podd A,Yu M,Liu HH,Wen R,Chen ZJ,Wang D

Published figure using IgM monoclonal antibody (Product # 50-5790-82) in Immunofluorescence

Thu May 07 00:00:00 EDT 2009

miR-19 is a key oncogenic component of mir-17-92.

Genes & development

Olive V,Bennett MJ,Walker JC,Ma C,Jiang I,Cordon-Cardo C,Li QJ,Lowe SW,Hannon GJ,He L

Published figure using IgM monoclonal antibody (Product # 50-5790-82) in Flow Cytometry

Tue Dec 15 00:00:00 EST 2009

Phospholipase Cgamma2 contributes to light-chain gene activation and receptor editing.

Molecular and cellular biology

Bai L,Chen Y,He Y,Dai X,Lin X,Wen R,Wang D

Published figure using IgM monoclonal antibody (Product # 50-5790-82) in Flow Cytometry

Sat Sep 01 00:00:00 EDT 2007

Bioinformatics

Gene Aliases: AI326478; Igh-6; Igh-M; Igh6; Igm; muH

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Entrez Gene ID: (Mouse) 16019

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Function(s)

immunoglobulin

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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IgM Monoclonal Antibody (II/41), eFluor™ 660, eBioscience™

IgM Antibody (50-5790-82) in ICC/IF

Product Details

50-5790-82

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

T-Cell Activation (TCA)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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