Invitrogen

CD62L (L-Selectin) Monoclonal Antibody (MEL-14), APC-eFluor™ 780, eBioscience™

CD62L (L-Selectin) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 64

CD62L (L-Selectin) Antibody (47-0621-82) in Flow

Staining of BALB/c splenocytes with Anti-Mouse CD3e FITC (Product # 11-0031-82) and 0.06 µg of Rat IgG2a K Isotype Control APC-eFluor® 780 (Product # 47-4321-82) (left) or 0.06 µg of Anti-Mouse CD62L (L-Selectin) APC-eFluor® 780 (right). Total viable cells were used for analysis.

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Product Details

47-0621-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.125 µg/test

View 72 publications 72 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), APC-eFluor™ 780, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

MEL-14

Conjugate

APC-eFluor™ 780 APC-eFluor™ 780 APC-eFluor™ 780

Excitation/Emission Max

756/785 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1603256

Product Specific Information

Description: The MEL-14 monoclonal antibody reacts with mouse CD62L, a 76 kDa member of the selectin family. CD62L is expressed by neutrophils, monocytes, and subsets of T, B, and NK cells and binds a number of glycosylated, fucosylated, sulfated sialylated glycoproteins including CD34, glycam-1 and MAdCam-1. These interactions mediate rolling of lymphocytes on activated endothelium at the sites of inflammation and homing of cells to the high endothelial venules (HEV) of peripheral lymphoid tissues.

Applications Reported: This MEL-14 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This MEL-14 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

APC-eFluor 780 emits at 780 nm and is excited with the Red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Light sensitivity: This tandem is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The human CD62L is a 74-95 kDa glycoprotein member of the selectin family of adhesion receptors. L-Selectin is comprised of an aminoterminal C-type lectin binding domain, an epidermal growth factor-like domain, two short consensus repeat (SCR) sequences homologous to those found in complement binding proteins, a short spacer region, a transmembrane region and a short cytoplasmic region. Human CD62L (L-Selectin) is constitutively expressed on all classes of leukocytes including lymphocytes (except a substantial population of memory T cells), monocytes and polymorphonuclear cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

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Zhang Q,Liang Z,Zhang J,Lei T,Dong X,Su H,Chen Y,Zhang Z,Tan L,Zhao Y

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Pulsatile MEK Inhibition Improves Anti-tumor Immunity and T Cell Function in Murine Kras Mutant Lung Cancer.

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Choi H,Deng J,Li S,Silk T,Dong L,Brea EJ,Houghton S,Redmond D,Zhong H,Boiarsky J,Akbay EA,Smith PD,Merghoub T,Wong KK,Wolchok JD

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Nature communications

Komban RJ,Strömberg A,Biram A,Cervin J,Lebrero-Fernández C,Mabbott N,Yrlid U,Shulman Z,Bemark M,Lycke N

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Liang Z,Zhang L,Su H,Luan R,Na N,Sun L,Zhao Y,Zhang X,Zhang Q,Li J,Zhang L,Zhao Y

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Cytokine- and TCR-Mediated Regulation of T Cell Expression of Ly6C and Sca-1.

Journal of immunology (Baltimore, Md. : 1950)

DeLong JH,Hall AO,Konradt C,Coppock GM,Park J,Harms Pritchard G,Hunter CA

47-0621-82 was used in Flow Cytometry to highlight the TCR-dependent and cytokine-mediated signals that modulate T cell expression of Ly6C and Sca-1 in vitro and in vivo during infection.

Thu Mar 01 00:00:00 EST 2018

C5aR1 regulates T follicular helper differentiation and chronic graft-versus-host disease bronchiolitis obliterans.

JCI insight

Verghese DA,Chun N,Paz K,Fribourg M,Woodruff TM,Flynn R,Hu Y,Xiong H,Zhang W,Yi Z,Du J,Blazar BR,Heeger PS

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Thu Dec 20 00:00:00 EST 2018

Helminth-induced IL-4 expands bystander memory CD8⁺ T cells for early control of viral infection.

Nature communications

Rolot M,Dougall AM,Chetty A,Javaux J,Chen T,Xiao X,Machiels B,Selkirk ME,Maizels RM,Hokke C,Denis O,Brombacher F,Vanderplasschen A,Gillet L,Horsnell WGC,Dewals BG

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Tue Oct 30 00:00:00 EDT 2018

SUMOylation of ROR-γt inhibits IL-17 expression and inflammation via HDAC2.

Nature communications

Singh AK,Khare P,Obaid A,Conlon KP,Basrur V,DePinho RA,Venuprasad K

47-0621 was used in Flow cytometry/Cell sorting to define the molecular mechanisms that govern the transcription factor activity of ROR-γt in the regulation of IL-17.

Tue Oct 30 00:00:00 EDT 2018

Stability and function of regulatory T cells expressing the transcription factor T-bet.

Nature

Levine AG,Mendoza A,Hemmers S,Moltedo B,Niec RE,Schizas M,Hoyos BE,Putintseva EV,Chaudhry A,Dikiy S,Fujisawa S,Chudakov DM,Treuting PM,Rudensky AY

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Thu Jun 15 00:00:00 EDT 2017

Antibody Tumor Targeting Is Enhanced by CD27 Agonists through Myeloid Recruitment.

Cancer cell

Turaj AH,Hussain K,Cox KL,Rose-Zerilli MJJ,Testa J,Dahal LN,Chan HTC,James S,Field VL,Carter MJ,Kim HJ,West JJ,Thomas LJ,He LZ,Keler T,Johnson PWM,Al-Shamkhani A,Thirdborough SM,Beers SA,Cragg MS,Glennie MJ,Lim SH

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Mon Dec 11 00:00:00 EST 2017

Excessive expression of miR-27 impairs Treg-mediated immunological tolerance.

The Journal of clinical investigation

Cruz LO,Hashemifar SS,Wu CJ,Cho S,Nguyen DT,Lin LL,Khan AA,Lu LF

Published figure using CD62L (L-Selectin) monoclonal antibody (Product # 47-0621-82) in Flow Cytometry

Wed Feb 01 00:00:00 EST 2017

Egr2 and 3 control adaptive immune responses by temporally uncoupling expansion from T cell differentiation.

The Journal of experimental medicine

Miao T,Symonds ALJ,Singh R,Symonds JD,Ogbe A,Omodho B,Zhu B,Li S,Wang P

Published figure using CD62L (L-Selectin) monoclonal antibody (Product # 47-0621-82) in Flow Cytometry

Mon Jun 05 00:00:00 EDT 2017

Peripheral administration of the soluble TNF inhibitor XPro1595 modifies brain immune cell profiles, decreases beta-amyloid plaque load, and rescues impaired long-term potentiation in 5xFAD mice.

Neurobiology of disease

MacPherson KP,Sompol P,Kannarkat GT,Chang J,Sniffen L,Wildner ME,Norris CM,Tansey MG

Published figure using CD62L (L-Selectin) monoclonal antibody (Product # 47-0621-82) in Flow Cytometry

Thu Jun 01 00:00:00 EDT 2017

Intestinal helminth infection induces highly functional resident memory CD4⁺ T cells in mice.

European journal of immunology

Steinfelder S,Rausch S,Michael D,Kühl AA,Hartmann S

Wed Feb 01 00:00:00 EST 2017

The Ox40/Ox40 Ligand Pathway Promotes Pathogenic Th Cell Responses, Plasmablast Accumulation, and Lupus Nephritis in NZB/W F1 Mice.

Journal of immunology (Baltimore, Md. : 1950)

Sitrin J,Suto E,Wuster A,Eastham-Anderson J,Kim JM,Austin CD,Lee WP,Behrens TW

Published figure using CD62L (L-Selectin) monoclonal antibody (Product # 47-0621-82) in Flow Cytometry

Tue Aug 15 00:00:00 EDT 2017

Batf3-dependent CD8α⁺ Dendritic Cells Aggravates Atherosclerosis via Th1 Cell Induction and Enhanced CCL5 Expression in Plaque Macrophages.

EBioMedicine

Li Y,Liu X,Duan W,Tian H,Zhu G,He H,Yao S,Yi S,Song W,Tang H

Published figure using CD62L (L-Selectin) monoclonal antibody (Product # 47-0621-82) in Flow Cytometry

Sat Apr 01 00:00:00 EDT 2017

Extrafollicular CD4⁺ T-B interactions are sufficient for inducing autoimmune-like chronic graft-versus-host disease.

Nature communications

Deng R,Hurtz C,Song Q,Yue C,Xiao G,Yu H,Wu X,Muschen M,Forman S,Martin PJ,Zeng D

Published figure using CD62L (L-Selectin) monoclonal antibody (Product # 47-0621-82) in Flow Cytometry

Tue Oct 17 00:00:00 EDT 2017

The immunosuppressive enzyme IL4I1 promotes FoxP3(+) regulatory T lymphocyte differentiation.

European journal of immunology

Cousin C,Aubatin A,Le Gouvello S,Apetoh L,Castellano F,Molinier-Frenkel V

Mon Jun 01 00:00:00 EDT 2015

Unexpected Role for Adaptive αβTh17 Cells in Acute Respiratory Distress Syndrome.

Journal of immunology (Baltimore, Md. : 1950)

Li JT,Melton AC,Su G,Hamm DE,LaFemina M,Howard J,Fang X,Bhat S,Huynh KM,O'Kane CM,Ingram RJ,Muir RR,McAuley DF,Matthay MA,Sheppard D

Published figure using CD62L (L-Selectin) monoclonal antibody (Product # 47-0621-82) in Flow Cytometry

Wed Jul 01 00:00:00 EDT 2015

Parasite Manipulation of the Invariant Chain and the Peptide Editor H2-DM Affects Major Histocompatibility Complex Class II Antigen Presentation during Toxoplasma gondii Infection.

Infection and immunity

Leroux LP,Nishi M,El-Hage S,Fox BA,Bzik DJ,Dzierszinski FS

47-0621 was used in Flow cytometry/Cell sorting to show that Toxoplasma gondii modulates the expression of key components of the MHC-II antigen presentation pathway in professional antigen-presenting cells.

Thu Oct 01 00:00:00 EDT 2015

Targeted Mutation of Nuclear Bone Morphogenetic Protein 2 Impairs Secondary Immune Response in a Mouse Model.

BioMed research international

Olsen DS,Goar WA,Nichols BA,Bailey KT,Christensen SL,Merriam KR,Reynolds PR,Wilson E,Weber KS,Bridgewater LC

Published figure using CD62L (L-Selectin) monoclonal antibody (Product # 47-0621-82) in Flow Cytometry

Mon Aug 01 00:00:00 EDT 2016

Successive site translocating inoculation potentiates DNA/recombinant vaccinia vaccination.

Scientific reports

Ren Y,Wang N,Hu W,Zhang X,Xu J,Wan Y

47-0621 was used in Flow cytometry/Cell sorting to identify further optimisations needed to get the immunogenicity of DNA vaccines closer to the level required for human use.

Tue Dec 15 00:00:00 EST 2015

Validation of a multicolor staining to monitor phosphoSTAT5 levels in regulatory T-cell subsets.

Oncotarget

Ehx G,Hannon M,Beguin Y,Humblet-Baron S,Baron F

47-0621 was used in Flow cytometry/Cell sorting to evaluate a novel staining method with the potential to monitor the responses of various Treg subsets to IL-2 therapy.

Tue Dec 22 00:00:00 EST 2015

Intravascular staining for discrimination of vascular and tissue leukocytes.

Nature protocols

Anderson KG,Mayer-Barber K,Sung H,Beura L,James BR,Taylor JJ,Qunaj L,Griffith TS,Vezys V,Barber DL,Masopust D

47-0621 was used in Flow cytometry/Cell sorting to outline a protocol for the validation and use of intravascular staining to define innate and adaptive immune cells in mice.

Wed Jan 01 00:00:00 EST 2014

T helper cell IL-4 drives intestinal Th2 priming to oral peanut antigen, under the control of OX40L and independent of innate-like lymphocytes.

Mucosal immunology

Chu DK,Mohammed-Ali Z,Jiménez-Saiz R,Walker TD,Goncharova S,Llop-Guevara A,Kong J,Gordon ME,Barra NG,Gillgrass AE,Van Seggelen H,Khan WI,Ashkar AA,Bramson JL,Humbles AA,Kolbeck R,Waserman S,Jordana M

47-0621 was used in Flow cytometry/Cell sorting to reveal intestinal Th2 priming is initiated by an autocrine/paracrine acting CD4(+) Th cell-intrinsic IL-4 program that is controlled by DC OX40L.

Sat Nov 01 00:00:00 EDT 2014

Myelin-specific T helper 17 cells promote adult hippocampal neurogenesis through indirect mechanisms.

F1000Research

Niebling J,E Rünker A,Schallenberg S,Kretschmer K,Kempermann G

Published figure using CD62L (L-Selectin) monoclonal antibody (Product # 47-0621-82) in Flow Cytometry

Fri Mar 06 00:00:00 EST 2020

STAT4 is required for IL-23 responsiveness in Th17 memory cells and NKT cells.

JAK-STAT

Glosson-Byers NL,Sehra S,Kaplan MH

Published figure using CD62L (L-Selectin) monoclonal antibody (Product # 47-0621-82) in Flow Cytometry

Mon Sep 28 00:00:00 EDT 2015

Establishment of a murine graft-versus-myeloma model using allogeneic stem cell transplantation.

PloS one

Binsfeld M,Beguin Y,Belle L,Otjacques E,Hannon M,Briquet A,Heusschen R,Drion P,Zilberberg J,Bogen B,Baron F,Caers J

47-0621 was used in Flow cytometry/Cell sorting to investigate the anti-myeloma effects of allo-SCT from B10.D2 mice into MHC-matched myeloma-bearing Balb/cJ mice.

Tue Dec 15 00:00:00 EST 2015

A disease-associated PTPN22 variant promotes systemic autoimmunity in murine models.

The Journal of clinical investigation

Dai X,James RG,Habib T,Singh S,Jackson S,Khim S,Moon RT,Liggitt D,Wolf-Yadlin A,Buckner JH,Rawlings DJ

Published figure using CD62L (L-Selectin) monoclonal antibody (Product # 47-0621-82) in Flow Cytometry

Wed May 01 00:00:00 EDT 2013

A role for LFA-1 in delaying T-lymphocyte egress from lymph nodes.

The EMBO journal

Reichardt P,Patzak I,Jones K,Etemire E,Gunzer M,Hogg N

47-0621 was used in Flow cytometry/Cell sorting to study the role of the leukocyte function-associated antigen-1 (LFA-1) during T cell migration within and away from the lymph nodes.

Wed Mar 20 00:00:00 EDT 2013

Phenotypic CD8+ T cell diversification occurs before, during, and after the first T cell division.

Journal of immunology (Baltimore, Md. : 1950)

Lemaître F,Moreau HD,Vedele L,Bousso P

47-0621 was used in Flow cytometry/Cell sorting to study the development of heterogeneous populations from single CD8(+) T cell progenies.

Thu Aug 15 00:00:00 EDT 2013

The cytokines interleukin 27 and interferon-γ promote distinct Treg cell populations required to limit infection-induced pathology.

Immunity

Hall AO,Beiting DP,Tato C,John B,Oldenhove G,Lombana CG,Pritchard GH,Silver JS,Bouladoux N,Stumhofer JS,Harris TH,Grainger J,Wojno ED,Wagage S,Roos DS,Scott P,Turka LA,Cherry S,Reiner SL,Cua D,Belkaid Y,Elloso MM,Hunter CA

47-0621 was used in Flow cytometry/Cell sorting to study the promotion of distinct Treg cell populations by the cytokines IL-27 and IFN-y, and the role they play in limiting infection induced pathology.

Fri Sep 21 00:00:00 EDT 2012

Hepatocyte-specific S100a8 and S100a9 transgene expression in mice causes Cxcl1 induction and systemic neutrophil enrichment.

Cell communication and signaling : CCS

Wiechert L,Németh J,Pusterla T,Bauer C,De Ponti A,Manthey S,Marhenke S,Vogel A,Klingmüller U,Hess J,Angel P

Published figure using CD62L (L-Selectin) monoclonal antibody (Product # 47-0621-82) in Flow Cytometry

Sat Dec 15 00:00:00 EST 2012

A role for IL-27 in limiting T regulatory cell populations.

Journal of immunology (Baltimore, Md. : 1950)

Wojno ED,Hosken N,Stumhofer JS,O'Hara AC,Mauldin E,Fang Q,Turka LA,Levin SD,Hunter CA

47-0621 was used in Flow cytometry/Cell sorting to show that mice that overexpress IL-27 had decreased Treg frequencies and developed spontaneous inflammation.

Fri Jul 01 00:00:00 EDT 2011

Modeling Sjögren's syndrome with Id3 conditional knockout mice.

Immunology letters

Guo Z,Li H,Han M,Xu T,Wu X,Zhuang Y

Published figure using CD62L (L-Selectin) monoclonal antibody (Product # 47-0621-82) in Flow Cytometry

Wed Mar 30 00:00:00 EDT 2011

IL-7 promotes T cell viability, trafficking, and functionality and improves survival in sepsis.

Journal of immunology (Baltimore, Md. : 1950)

Unsinger J,McGlynn M,Kasten KR,Hoekzema AS,Watanabe E,Muenzer JT,McDonough JS,Tschoep J,Ferguson TA,McDunn JE,Morre M,Hildeman DA,Caldwell CC,Hotchkiss RS

Published figure using CD62L (L-Selectin) monoclonal antibody (Product # 47-0621-82) in Flow Cytometry

Thu Apr 01 00:00:00 EDT 2010

Modulation of naive CD4+ T-cell responses to an airway antigen during pulmonary mycobacterial infection.

Infection and immunity

Anis MM,Fulton SA,Reba SM,Harding CV,Boom WH

Published figure using CD62L (L-Selectin) monoclonal antibody (Product # 47-0621-82) in Flow Cytometry

Tue May 01 00:00:00 EDT 2007

Bioinformatics

Protein Aliases: CD62 antigen-like family member L; CD62L; hLHRc; L-selectin; LAM-1; LECAM1; Leu-8; Leukocyte adhesion molecule 1; Leukocyte-endothelial cell adhesion molecule 1; Ly-22; Lymph node homing receptor; Lymphocyte antigen 22; Lymphocyte surface MEL-14 antigen

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Gene Aliases: AI528707; CD62L; L-selectin; LECAM-1; Lnhr; Ly-22; Ly-m22; Ly22; Lyam-1; Lyam1; Sell

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UniProt ID: (Mouse) P18337

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Entrez Gene ID: (Mouse) 20343

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Function(s)

protease binding protein binding carbohydrate binding cell adhesion molecule binding glycolipid binding

Process(es)

cell adhesion response to ATP regulation of apoptotic process positive regulation of neutrophil chemotaxis

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CD62L (L-Selectin) Monoclonal Antibody (MEL-14), APC-eFluor™ 780, eBioscience™

CD62L (L-Selectin) Antibody (47-0621-82) in Flow

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47-0621-82

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