Invitrogen

CD62L (L-Selectin) Monoclonal Antibody (MEL-14), Alexa Fluor™ 700, eBioscience™

CD62L (L-Selectin) Antibody in Flow Cytometry (Flow)

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CD62L (L-Selectin) Antibody (56-0621-82) in Flow

Staining of BALB/c splenocytes with 0.03 µg of Rat IgG2a K Isotype Control Alexa Fluor® 700 (Product # 56-4321-80) (open histogram) or 0.03 µg of Anti-Mouse CD62L (L-selectin) Alexa Fluor® 700 (filled histogram). Cells in the lymphocyte gate were used for analysis.

CD62L (L-Selectin) Antibody in Immunocytochemistry (ICC/IF)

Product Image Gallery

Product Details

56-0621-82

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

0.06 µg/test

View 58 publications 58 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), Alexa Fluor™ 700, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

MEL-14

Conjugate

Alexa Fluor™ 700 Alexa Fluor™ 700 Alexa Fluor™ 700

Excitation/Emission Max

696/719 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_494003

Product Specific Information

Description: The MEL-14 monoclonal antibody reacts with mouse CD62L, a 76 kDa member of the selectin family. CD62L is expressed by neutrophils, monocytes, and subsets of T, B, and NK cells and binds a number of glycosylated, fucosylated, sulfated sialylated glycoproteins including CD34, glycam-1 and MAdCam-1. These interactions mediate rolling of lymphocytes on activated endothelium at the sites of inflammation and homing of cells to the high endothelial venules (HEV) of peripheral lymphoid tissues.

Applications Reported: This MEL-14 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This MEL-14 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Alexa Fluor® 700 emits at 723 nm and can be excited with the red laser (633 nm). Most instruments will require a 685 LP mirror and 710/20 filter. Please make sure that your instrument is capable of detecting this fluorochrome.

Excitation: 633-647 nm; Emission: 723 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The human CD62L is a 74-95 kDa glycoprotein member of the selectin family of adhesion receptors. L-Selectin is comprised of an aminoterminal C-type lectin binding domain, an epidermal growth factor-like domain, two short consensus repeat (SCR) sequences homologous to those found in complement binding proteins, a short spacer region, a transmembrane region and a short cytoplasmic region. Human CD62L (L-Selectin) is constitutively expressed on all classes of leukocytes including lymphocytes (except a substantial population of memory T cells), monocytes and polymorphonuclear cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

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Journal of neurovirology

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Synchronous effects of targeted mitochondrial complex I inhibitors on tumor and immune cells abrogate melanoma progression.

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Theranostic near-infrared-IIb emitting nanoprobes for promoting immunogenic radiotherapy and abscopal effects against cancer metastasis.

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Targeting Phosphatidylserine Enhances the Anti-tumor Response to Tumor-Directed Radiation Therapy in a Preclinical Model of Melanoma.

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Type 1 Cytotoxic T Cells Increase in Placenta after Intrauterine Inflammation.

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Near-infrared photoimmunotherapy targeting human-EGFR in a mouse tumor model simulating current and future clinical trials.

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Inhibition of TLR4 signaling protects mice from sensory and motor dysfunction in an animal model of autoimmune peripheral neuropathy.

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STING Activated Tumor-Intrinsic Type I Interferon Signaling Promotes CXCR3 Dependent Antitumor Immunity in Pancreatic Cancer.

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A nanounit strategy reverses immune suppression of exosomal PD-L1 and is associated with enhanced ferroptosis.

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Attenuation of TCR-induced transcription by Bach2 controls regulatory T cell differentiation and homeostasis.

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Fri Mar 22 00:00:00 EDT 2019

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JCI insight

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Thu Dec 20 00:00:00 EST 2018

Helminth-induced IL-4 expands bystander memory CD8⁺ T cells for early control of viral infection.

Nature communications

Rolot M,Dougall AM,Chetty A,Javaux J,Chen T,Xiao X,Machiels B,Selkirk ME,Maizels RM,Hokke C,Denis O,Brombacher F,Vanderplasschen A,Gillet L,Horsnell WGC,Dewals BG

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Tue Oct 30 00:00:00 EDT 2018

Stability and function of regulatory T cells expressing the transcription factor T-bet.

Nature

Levine AG,Mendoza A,Hemmers S,Moltedo B,Niec RE,Schizas M,Hoyos BE,Putintseva EV,Chaudhry A,Dikiy S,Fujisawa S,Chudakov DM,Treuting PM,Rudensky AY

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Thu Jun 15 00:00:00 EDT 2017

Antibody Tumor Targeting Is Enhanced by CD27 Agonists through Myeloid Recruitment.

Cancer cell

Turaj AH,Hussain K,Cox KL,Rose-Zerilli MJJ,Testa J,Dahal LN,Chan HTC,James S,Field VL,Carter MJ,Kim HJ,West JJ,Thomas LJ,He LZ,Keler T,Johnson PWM,Al-Shamkhani A,Thirdborough SM,Beers SA,Cragg MS,Glennie MJ,Lim SH

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Mon Dec 11 00:00:00 EST 2017

Excessive expression of miR-27 impairs Treg-mediated immunological tolerance.

The Journal of clinical investigation

Cruz LO,Hashemifar SS,Wu CJ,Cho S,Nguyen DT,Lin LL,Khan AA,Lu LF

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Wed Feb 01 00:00:00 EST 2017

Egr2 and 3 control adaptive immune responses by temporally uncoupling expansion from T cell differentiation.

The Journal of experimental medicine

Miao T,Symonds ALJ,Singh R,Symonds JD,Ogbe A,Omodho B,Zhu B,Li S,Wang P

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Mon Jun 05 00:00:00 EDT 2017

Peripheral administration of the soluble TNF inhibitor XPro1595 modifies brain immune cell profiles, decreases beta-amyloid plaque load, and rescues impaired long-term potentiation in 5xFAD mice.

Neurobiology of disease

MacPherson KP,Sompol P,Kannarkat GT,Chang J,Sniffen L,Wildner ME,Norris CM,Tansey MG

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Thu Jun 01 00:00:00 EDT 2017

The Ox40/Ox40 Ligand Pathway Promotes Pathogenic Th Cell Responses, Plasmablast Accumulation, and Lupus Nephritis in NZB/W F1 Mice.

Journal of immunology (Baltimore, Md. : 1950)

Sitrin J,Suto E,Wuster A,Eastham-Anderson J,Kim JM,Austin CD,Lee WP,Behrens TW

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Tue Aug 15 00:00:00 EDT 2017

Batf3-dependent CD8α⁺ Dendritic Cells Aggravates Atherosclerosis via Th1 Cell Induction and Enhanced CCL5 Expression in Plaque Macrophages.

EBioMedicine

Li Y,Liu X,Duan W,Tian H,Zhu G,He H,Yao S,Yi S,Song W,Tang H

Published figure using CD62L (L-Selectin) monoclonal antibody (Product # 56-0621-82) in Flow Cytometry

Sat Apr 01 00:00:00 EDT 2017

Extrafollicular CD4⁺ T-B interactions are sufficient for inducing autoimmune-like chronic graft-versus-host disease.

Nature communications

Deng R,Hurtz C,Song Q,Yue C,Xiao G,Yu H,Wu X,Muschen M,Forman S,Martin PJ,Zeng D

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Tue Oct 17 00:00:00 EDT 2017

A Mouse Model of West Nile Virus Infection.

Current protocols in mouse biology

Graham JB,Swarts JL,Lund JM

56-0621 was used in Flow cytometry/Cell sorting to develop a mouse model to study the breadth of symptoms and disease severity seen in human West Nile virus infection.

Wed Dec 20 00:00:00 EST 2017

BTK inhibition results in impaired CXCR4 chemokine receptor surface expression, signaling and function in chronic lymphocytic leukemia.

Leukemia

Chen SS,Chang BY,Chang S,Tong T,Ham S,Sherry B,Burger JA,Rai KR,Chiorazzi N

56-0621 was used in Flow cytometry/Cell sorting to identify CXCR4 as a key regulator in BTK-mediated CLL-cell retention.

Fri Apr 01 00:00:00 EDT 2016

CD8+ T Cells Can Mediate Short-Term Protection against Heterotypic Dengue Virus Reinfection in Mice.

Journal of virology

Zellweger RM,Tang WW,Eddy WE,King K,Sanchez MC,Shresta S

56-0621 was used in Flow cytometry/Cell sorting to investigate short-term protection against heterotypic Dengue virus reinfection in mice mediated by CD8+ T Cells.

Mon Jun 01 00:00:00 EDT 2015

Unexpected Role for Adaptive αβTh17 Cells in Acute Respiratory Distress Syndrome.

Journal of immunology (Baltimore, Md. : 1950)

Li JT,Melton AC,Su G,Hamm DE,LaFemina M,Howard J,Fang X,Bhat S,Huynh KM,O'Kane CM,Ingram RJ,Muir RR,McAuley DF,Matthay MA,Sheppard D

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Wed Jul 01 00:00:00 EDT 2015

Targeted Mutation of Nuclear Bone Morphogenetic Protein 2 Impairs Secondary Immune Response in a Mouse Model.

BioMed research international

Olsen DS,Goar WA,Nichols BA,Bailey KT,Christensen SL,Merriam KR,Reynolds PR,Wilson E,Weber KS,Bridgewater LC

Published figure using CD62L (L-Selectin) monoclonal antibody (Product # 56-0621-82) in Flow Cytometry

Mon Aug 01 00:00:00 EDT 2016

Large T Antigen-Specific Cytotoxic T Cells Protect Against Dendritic Cell Tumors through Perforin-Mediated Mechanisms Independent of CD4 T Cell Help.

Frontiers in immunology

Duval A,Fuertes Marraco SA,Schwitter D,Leuenberger L,Acha-Orbea H

56-0621 was used in Flow cytometry/Cell sorting to observe that after transfer into syngeneic C57BL/6 mice, MuTuDC lines were quickly rejected.

Thu Aug 07 00:00:00 EDT 2014

CD8+ T cells prevent antigen-induced antibody-dependent enhancement of dengue disease in mice.

Journal of immunology (Baltimore, Md. : 1950)

Zellweger RM,Eddy WE,Tang WW,Miller R,Shresta S

56-0621 was used in Flow cytometry/Cell sorting to investigate how CD8(+) T cell responses influence the outcome of antibody-mediated severe dengue disease.

Wed Oct 15 00:00:00 EDT 2014

CCR1 inhibition ameliorates the progression of lupus nephritis in NZB/W mice.

Journal of immunology (Baltimore, Md. : 1950)

Bignon A,Gaudin F,Hémon P,Tharinger H,Mayol K,Walzer T,Loetscher P,Peuchmaur M,Berrebi D,Balabanian K

56-0621 was used in Flow cytometry/Cell sorting to define a pivotal role for CCR4 in the recruitment of T and mononuclear phagocyte cells to inflamed kidneys of lupus-prone mice.

Sat Feb 01 00:00:00 EST 2014

Myelin-specific T helper 17 cells promote adult hippocampal neurogenesis through indirect mechanisms.

F1000Research

Niebling J,E Rünker A,Schallenberg S,Kretschmer K,Kempermann G

Published figure using CD62L (L-Selectin) monoclonal antibody (Product # 56-0621-82) in Flow Cytometry

Fri Mar 06 00:00:00 EST 2020

STAT4 is required for IL-23 responsiveness in Th17 memory cells and NKT cells.

JAK-STAT

Glosson-Byers NL,Sehra S,Kaplan MH

Published figure using CD62L (L-Selectin) monoclonal antibody (Product # 56-0621-82) in Flow Cytometry

Mon Sep 28 00:00:00 EDT 2015

A disease-associated PTPN22 variant promotes systemic autoimmunity in murine models.

The Journal of clinical investigation

Dai X,James RG,Habib T,Singh S,Jackson S,Khim S,Moon RT,Liggitt D,Wolf-Yadlin A,Buckner JH,Rawlings DJ

Published figure using CD62L (L-Selectin) monoclonal antibody (Product # 56-0621-82) in Flow Cytometry

Wed May 01 00:00:00 EDT 2013

Hepatocyte-specific S100a8 and S100a9 transgene expression in mice causes Cxcl1 induction and systemic neutrophil enrichment.

Cell communication and signaling : CCS

Wiechert L,Németh J,Pusterla T,Bauer C,De Ponti A,Manthey S,Marhenke S,Vogel A,Klingmüller U,Hess J,Angel P

Published figure using CD62L (L-Selectin) monoclonal antibody (Product # 56-0621-82) in Flow Cytometry

Sat Dec 15 00:00:00 EST 2012

Modeling Sjögren's syndrome with Id3 conditional knockout mice.

Immunology letters

Guo Z,Li H,Han M,Xu T,Wu X,Zhuang Y

Published figure using CD62L (L-Selectin) monoclonal antibody (Product # 56-0621-82) in Flow Cytometry

Wed Mar 30 00:00:00 EDT 2011

IL-7 promotes T cell viability, trafficking, and functionality and improves survival in sepsis.

Journal of immunology (Baltimore, Md. : 1950)

Unsinger J,McGlynn M,Kasten KR,Hoekzema AS,Watanabe E,Muenzer JT,McDonough JS,Tschoep J,Ferguson TA,McDunn JE,Morre M,Hildeman DA,Caldwell CC,Hotchkiss RS

Published figure using CD62L (L-Selectin) monoclonal antibody (Product # 56-0621-82) in Flow Cytometry

Thu Apr 01 00:00:00 EDT 2010

Modulation of naive CD4+ T-cell responses to an airway antigen during pulmonary mycobacterial infection.

Infection and immunity

Anis MM,Fulton SA,Reba SM,Harding CV,Boom WH

Published figure using CD62L (L-Selectin) monoclonal antibody (Product # 56-0621-82) in Flow Cytometry

Tue May 01 00:00:00 EDT 2007

Bioinformatics

Protein Aliases: CD62 antigen-like family member L; CD62L; hLHRc; L-selectin; LAM-1; LECAM1; Leu-8; Leukocyte adhesion molecule 1; Leukocyte-endothelial cell adhesion molecule 1; Ly-22; Lymph node homing receptor; Lymphocyte antigen 22; Lymphocyte surface MEL-14 antigen

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Gene Aliases: AI528707; CD62L; L-selectin; LECAM-1; Lnhr; Ly-22; Ly-m22; Ly22; Lyam-1; Lyam1; Sell

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UniProt ID: (Mouse) P18337

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Entrez Gene ID: (Mouse) 20343

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Function(s)

protease binding protein binding carbohydrate binding cell adhesion molecule binding glycolipid binding

Process(es)

cell adhesion response to ATP regulation of apoptotic process positive regulation of neutrophil chemotaxis

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CD62L (L-Selectin) Monoclonal Antibody (MEL-14), Alexa Fluor™ 700, eBioscience™

CD62L (L-Selectin) Antibody (56-0621-82) in Flow

Product Details

56-0621-82

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

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Published species

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Type

Clone

Conjugate

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Contains

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